LOCUS       sequence-208208-       13130 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13130)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 13130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU70.1-2.2
FEATURES             Location/Qualifiers
     source          1..13130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             504..527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(7565..7585)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7565..7583)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(7922..8510)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8002..8021)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8681..9472,9473..9541))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9304..9323
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9542..9646)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9673..11015
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10636..10683)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     11056..11074
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11346..11750
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             11763..12857
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(13105..13121)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13105..13121)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggattata aggatgatga tgataagact agtatggatg
      541 attcagtcca gccaaacccc tccatgcctc ctatactagt agggtccacg caagcttcgt
      601 gccaactatt cctctttggg gatctaacca catctttcga gcaagacctt cgtcgacttc
      661 ttcatgtcaa agataatgct accttacatt ccttcttcga gaaagtcagt gctgcctttc
      721 gtacggaatt tgggaaactc cctgctcgtc agcaagcctt gcttcctcgc tttaccacca
      781 ttaccgatct tcttgcaaat ttgggtgaga cagaaggaaa gccagccttg agtttcgctc
      841 tccactgtct ctgtgagatt ggtcaattca ttagatattt tggagaagga tccagacctt
      901 atcctagcga tgccaacacc tatcttgtgg cactgtgcac tggctcgttt gctgccgctg
      961 caatcagtac ctctcgaaca ctttcagagt tgattcccgc aggaatcgaa gccgttcttg
     1021 cagccttcag gaccggtctg tgctcgctgg agcttcgaaa tgatatagag cggccgagca
     1081 gtcgaatacc tcgatcttgg tccgcgattg tgagtgcttc agaagcacag gccgctgaac
     1141 ttctgcagag tttcaactca aaaaacactc tatctggaag ctccaggctt tttctaagcg
     1201 ccgtttcgcc aaacagtata acaatcagtg ggcctccaag cattctacac aactttctgg
     1261 cttcagtttc tttgaactcg tattgcctac cgaacgagtc gccgttccat gctccttccc
     1321 tgtttgggtc ttatgaggtt aatgagataa ttagctactt ccctgatgaa ttgcttggtt
     1381 catacaagcc gcgaattcct ctgttatctg ctgcttccgg caaacctatc gtcgctgagg
     1441 acttcaagac cctgctgcat acggttgtgt cagacactct gtgcgagcag gttcgatggg
     1501 acaacatcct ccggtcgctc gtggaagatg tcttccagca gaagtccttc aaagaatgca
     1561 caatatcacc cgtattgtct aatgctgctc acctcctctc ttcagcaata acgcgcgcca
     1621 caaaaatcaa tgtcacaacg accaatttgt tgaatctcgg cgtctcggac tcccagcctg
     1681 ccaggcctac tggtaaattc gagcagtcaa agatagccat cactgggttt tcaggtcgtt
     1741 tcccggaagc tgcatcgaat gaggaatttt gggagctgct aagtgcagga cgagacgtcc
     1801 atcgcacaat tcctgaagat cgcttcaact ggaaaactca cttcgatgca gcaggtaagg
     1861 taaagaacac aagcagggtt cagtatggat gcttcatcaa tgagccagga ttatttgatg
     1921 ctcgattttt caacatgtcc cccagagaat ctcaaaacac agatccagct cagaggttag
     1981 ccatcacgac aacatacgag gctatagaaa tggcaggatt ggtccctaac aggacacctt
     2041 ccacacaaca agaccgcatt ggtgtctttt ttggggtgac tagtgatgat tggcgagagg
     2101 tcaatagcgg ccaaaacatc gacacatatt ttatccctgg cggaaaccgt gcatttgtcc
     2161 ccggacgcat aagctacttc tttagatttt ctggtccaag cctgagtatc gatacagcat
     2221 gttcctcaag cttcgctgca atccaaacgg cgtgtaacta tttatggaga ggagaatgtg
     2281 atactgcagt tgccggagga accaatgtac tcaccaatcc tgacaacttt gctggccttg
     2341 atcgtggaca cttcctatcg accacaggaa actgtaatgc ctttgatgac ggtgctagcg
     2401 gctactgcag agcagacgcc gtggggtccg tcattctcaa gaggctcgaa gatgcgcaag
     2461 cggacaacga tccaatcttt ggggtcatcg atggagcttt tacaaatcat tgtggtcaaa
     2521 cagaatccat cacaagacct cacgagggcg accaagcttc cgtctttaac cggatcatga
     2581 ggaattccaa cgtcaatcca ctggatgtta gctacataga aatgcatgga actggaaccc
     2641 aagcaggaga tgctactgag atgaattcag ttctatcagt tttcgtgcct ggtcgcgagc
     2701 gaatgccaca ccacccgcta tatcttgggt ccgcgaaagc caacgttggt cacgcagagg
     2761 cagccagtgg tgtttctgct ttgatcaagg tcctgatgat gatgaagaac aaccagatac
     2821 caccgcattg cggaatcaaa actaagatca accgtaacta tcctcttgat ctcaaagagc
     2881 gcaatgtcaa cattgcgttg aaaactgtcc cttggcatcg aactgattgc gtcggtggga
     2941 agcgcacatg ttttctgaac aatttcagcg ccgctggagg taacacagcc gtgctcctag
     3001 aagacgctcc gttacctcaa tcagaaaaca cagcaataga gactcgctca gtacatcccg
     3061 tcatagtgac tgcaaagagt gtacagtctc tgaaacaaaa tatcgagtcc ttgatcacct
     3121 ttcttgagaa gaacccgagc acctcgcttc catcattatc atacactact acggcacgac
     3181 gaattcacca caattaccgc accatctgct cagggtcaga catcgaatcc attcgggacg
     3241 ctctcaggca gaaagccagc agttccgaca tcaagcctat tccaaatgta accaagcttc
     3301 cgaaggtggc cttcgtcttc acaggtcaag gctgtctgta tgctggtgtt ggcaaacagc
     3361 tattcgagac tgtctcgcat ttcagagctg atattcttcg gttcgacaac atagctcagc
     3421 ggcaaggttt cccaagcttt ttgcctgtgg tcgatggtag cgtcgccaat gttggtgagc
     3481 tagagccagt ggtttcccat ttggcagtga catgcgtgca aatggcgctt tctcgcctct
     3541 ggatctcttg gggtgtggtt ccgagtttga caactggcca cagcctggga gaatacgccg
     3601 ccctctatgc ggctggggtc gtgacagcca gcgacgccat tcaccttgtt ggtaccagag
     3661 cacagttgct atcgaagcat tgcaataaag gaacacatac tatgctggcc atcaaagcat
     3721 ctctcgactc catcaaaccc cgtctaagcg cctccgtctg cgaagtcgct tgtttcaacc
     3781 agcctgccag caatgtgatc agcggaccca gcgaagaaat cagccgctta atgagggaat
     3841 atcaatcaga cggtttcgaa tgtatcgagc tagacatacc gttcgctttt cattcagcac
     3901 aagttgagcc tatcttggag ctattcgaga gggcagccaa cggcgtccag ttccatgccc
     3961 cttcaattcc ctacatgtcg cctttgctgg gccgcattat ctcagatggt gatgtgttag
     4021 gaccatcata ccttgctcga gcatgccgca atgcagtaaa cttccaaggt gctcttcagg
     4081 ctgcgaaagc ttcatctgtt gtcaacgaga agtcagtctg gttggaaatt ggatcgcatc
     4141 cagcttgttc tggcatgatc aagggaacgc tgggcacaca tgccttgaca ataccgtctc
     4201 ttaaaaaagc tacagacacg tggaaagtgc tagccagcgg gctcgaatca ctttatctca
     4261 gcggcattga gatcgattgg aatgaatatc accgagactt tgagagctcg cataaggttc
     4321 tagagcttcc aaggtactgt tgggatttga agaattattg gatccagtac aggaatgact
     4381 tctgcctcac caaaggggac gatccaccac cacaaaaaga aattggtgct gctcctgctg
     4441 ttgagcagaa gcctgtgcct ctctatattt caccctctgt tcaacgtatt cttgaggagc
     4501 acaatgcagc tgatgtgtca actcttctcg ccgaatcaga catacacgat ccgcgacttg
     4561 ctcccatctt ccaaggccac aacgtgaatg gtacagctct atgtccttca tctctgtatg
     4621 ccgacatcgc cctgacggtc gttgattaca tgctgaagac aaacgatatg caaaatgata
     4681 ctactggcct tgacgtgggc gacgtgaagg tcgacaaatc gcttatagct gattctaacg
     4741 ctgtgacaca actacttcgt atttcggcgt ccgcaaattg gtcaaccaaa gtcgtttcac
     4801 ttgccctcta cagcattaac gcacagggca gaaagttagc cgatcacgcg aaatgccagg
     4861 tccggatcac tgaaaagcag tcatggtttc aagagtggaa gcgcagctcg tatctcatca
     4921 agagcagagt cacatcactt tacaaaggag tcgatggagg cgattcgcac cagatcaagc
     4981 gtggcatggt ctacaagctg ttctcttcca ttgtggacta tgatgcaaga tatcaagcca
     5041 tgcaagaggt cgtactcgat agcaacgagc tcgaagctac cgcacaggtg tcattccagg
     5101 ttgatgatga aggattcaac ttcaaccctt gttggattga cggccttggg catatcgctg
     5161 ggttcatcat gaatggaaat gacaatgttc actcgaagga ccaggtgttt atcaatcatg
     5221 gctgggatac catgcgatgc gcgacgaagt tcaggaaggg caagacttac cgaagctaca
     5281 acaaaatgca gcttgtgagt ggatcattat acgcgggcga tacgtatatt cttgaggacg
     5341 acaacgttgt tgctgtcatt gaaggtgtca agttcgctgg aataccgcgc caagtcctga
     5401 accaggtatt gcctccaaag tctggagcag caggacccgc agtgactaaa tcgcgttctt
     5461 caacgcaatc aaaaccggtg ccgatggcca cagccaatcc gcttccaaaa tcaactcgca
     5521 cagccacggc tactccgctc cccaaacaag caatatcacc accaaactcc aatacaaagc
     5581 ataccaaagc gtcaccgacg aatagcattg tgcccctagt attggcaatc attgcagagg
     5641 aggctggcct cgacgcggcc gagcttggac caagcagcga atttgccgat tatggcattg
     5701 actcattgtt gtccctcacc ataactggga gaatacaaga agagctcgga ctcgatctac
     5761 cctcttcact ctttgtggac cagcctactg tgaaggaatt tacagtgttc cttgatagta
     5821 gcaacaacag gcccttgttg tcaagtcctt ccggcaactc gacgccagtc tcaaatccag
     5881 agcaagaaac cctttcggaa gctacgccgg aagactacga aacagacgca acatcagtta
     5941 cagacggcca aagcgtgatg gaaatcatcc gcgccacgat cgctgcagag actggtgtct
     6001 ctcttgagga cttgacacct tcgacgagtt tctcagagct tggtgtagat tctctgctag
     6061 cgctcacgat catgggaaag cttggtgaag tcttagaaac ggatcttccg caaacacttc
     6121 ttgccgataa cgacaatctg tccgagatcg aaaaggcatt gagcctgaag ctgaagggac
     6181 cagacctcaa ccgaaatcct gtggctactt ctccaagagc ggacccgttc gaccccgaga
     6241 tgcctcctca cgccacatca gtcaagctcc aagggaatcc caagaatgcc acaaagacaa
     6301 tattcctctt tccagatggt tctggatcag ctacctcgta cgcttcaatt cctaaactct
     6361 cgcccgagat cgtggtgtat ggcttgaact gtccctggat gaagacacca caggatatga
     6421 aatgctcctt ggaacacctg acaagcaaat atattatcga gatccgccgt cggcagccaa
     6481 aaggccctta ttactttggt ggttggtcgg cgggaggaat atgcgcattt gaagctgcgc
     6541 aacagctggc cagccagggt gaagagacta gacgactgat attgattgat tcaccaaatc
     6601 cagtcggctt ggagaatccg cctcagcgca tgtacgactt ctttgaaagc ttggactttt
     6661 tcggcatgaa tggcaaggcg gctccaagct ggctacggcc ccatttcaat gccttcatca
     6721 gcacgctgga tcattacaag gtgaaacctt tttcaggtcc acggcttgag actcacctaa
     6781 tctacgctcg tgatggaatt tgcaaacatc ccagtgatcc tcgaccggag attcgccccg
     6841 atgatcctcg ggagatgctc tggcttctca acaatcgcac tgatttcagt ggcaggggct
     6901 gggatgactt ggttgggaag gagaacctga agatccaagt gttggacgag gtcaatcatt
     6961 tttcgatggt ggcgcctggt ccgaaaattc aagagctcgg tgctttcatc gcacgtgcaa
     7021 tggagtgagg agattgataa gacttttcta gttgcatatc ttttatattt aaatcttatc
     7081 tattagttaa ttttttgtaa tttatcctta tatatagtct ggttattcta aaatatcatt
     7141 tcagtatcta aaaattcccc tcttttttca gttatatctt aacaggcgac agtccaaatg
     7201 ttgatttatc ccagtccgat tcatcagggt tgtgaagcat tttgtcaatg gtcgaaatca
     7261 catcagtaat agtgcctctt acttgcctca tagaatttct ttctcttaac gtcaccgttt
     7321 ggtcttttat agtttcgaaa tctatggtga taccaaatgg tgttcccaat tcatcgttac
     7381 gggcgtattt tttaccaatt gaagtattgg aatcgtcaat tttaaagtat atctctcttt
     7441 tacgtaaagc ctgcgagatc ctcttaagta tagcggggaa gccatcgtta ttcgatattg
     7501 tcgtaacaaa tactttgatc ggcgctatgc ggccgccacc gcggtggagc tccagctttt
     7561 gttcccttta gtgagggtta attgcgcgct tggcgagact caaacaagat atattttgcc
     7621 cgccttacag acgaaactag ttggaggtta tggagcatac tatcacgtgg gcggccactg
     7681 gtgagttact acaccccagg ggcaacgttg atgctcctaa aaaactctgg ctggacgcaa
     7741 gccgtaacac ccgtgtcact tcataatcgt ttgcaattca gggcttgatc tacacattac
     7801 cctgttatcc ctattaatta atacggttat ccacagaatc aggggataac gcaggaaaga
     7861 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7921 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7981 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8041 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8101 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8161 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8221 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8281 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8341 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     8401 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8461 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8521 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8581 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     8641 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     8701 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     8761 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     8821 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     8881 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     8941 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9001 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9061 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9121 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9181 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9241 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9301 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9361 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9421 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9481 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9541 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     9601 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     9661 aagtgccacc tgaacgaagc atctgtgctt cattttgtag aacaaaaatg caacgcgaga
     9721 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg
     9781 aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg
     9841 cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca
     9901 acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc tacaaaaatg
     9961 catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt ctcctttgtg
    10021 cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa ggttagaaga
    10081 aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt
    10141 tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc cccgattata
    10201 ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg ttgatgattc
    10261 ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata ctacgtatag
    10321 gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct tactacaatt
    10381 tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc gagtttagat
    10441 gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc acagagatat
    10501 atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat attttagtag
    10561 ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt
    10621 tttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggaac
    10681 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag
    10741 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag
    10801 aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat
    10861 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt
    10921 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg attagtctca
    10981 tccttcaatg ctatcatttc ctttgatatt ggatcatact aagaaaccat tattatcatg
    11041 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
    11101 gacggtgaaa acctcggcgc gccattaccc tgttatccct aatatacaac gcaaagaagc
    11161 tggaaaattg gcgagggaat cctgtttctg tctatccaag aatgggcatg aggtggcaac
    11221 cgtcgtgcta gcgtacaggg tgcactttgt aaccatttgg gacaccggac actcgctgtt
    11281 ttcgaaatta ccctttaagc gcgggtattg aaccaggctt atgcccaaga tcgtagcaag
    11341 catttaactg tgggaatact caggtatcgt aagatgcaag agttcgaatc tcttagcaac
    11401 cattattttt ttcctcaaca taacgagaac acacaggggc gctatcgcac agaatcaaat
    11461 tcgatgactg gaaatttttt gttaatttca gaggtcgcct gacgcatata cctttttcaa
    11521 ctgaaaaatt gggagaaaaa ggaaaggtga gaggccggaa ccggcttttc atatagaata
    11581 gagaagcgtt catgactaaa tgcttgcatc acaatacttg aagttgacaa tattatttaa
    11641 ggacctattg ttttttccaa taggtggtta gcaatcgtct tactttctaa cttttcttac
    11701 cttttacatt tcagcaatat atatatatat ttcaaggata taccattcta atgtctgccc
    11761 ctatgtctgc ccctaagaag atcgtcgttt tgccaggtga ccacgttggt caagaaatca
    11821 cagccgaagc cattaaggtt cttaaagcta tttctgatgt tcgttccaat gtcaagttcg
    11881 atttcgaaaa tcatttaatt ggtggtgctg ctatcgatgc tacaggtgtc ccacttccag
    11941 atgaggcgct ggaagcctcc aagaaggttg atgccgtttt gttaggtgct gtggctggtc
    12001 ctaaatgggg taccggtagt gttagacctg aacaaggttt actaaaaatc cgtaaagaac
    12061 ttcaattgta cgccaactta agaccatgta actttgcatc cgactctctt ttagacttat
    12121 ctccaatcaa gccacaattt gctaaaggta ctgacttcgt tgttgtcaga gaattagtgg
    12181 gaggtattta ctttggtaag agaaaggaag acgatggtga tggtgtcgct tgggatagtg
    12241 aacaatacac cgttccagaa gtgcaaagaa tcacaagaat ggccgctttc atggccctac
    12301 aacatgagcc accattgcct atttggtcct tggataaagc taatcttttg gcctcttcaa
    12361 gattatggag aaaaactgtg gaggaaacca tcaagaacga attccctaca ttgaaggttc
    12421 aacatcaatt gattgattct gccgccatga tcctagttaa gaacccaacc cacctaaatg
    12481 gtattataat caccagcaac atgtttggtg atatcatctc cgatgaagcc tccgttatcc
    12541 caggttcctt gggtttgttg ccatctgcgt ccttggcctc tttgccagac aagaacaccg
    12601 catttggttt gtacgaacca tgccacggtt ctgctccaga tttgccaaag aataaggttg
    12661 accctatcgc cactatcttg tctgctgcaa tgatgttgaa attgtcattg aacttgcctg
    12721 aagaaggtaa ggccattgaa gatgcagtta aaaaggtttt ggatgcaggt atcagaactg
    12781 gtgatttagg tggttccaac agtaccaccg aagtcggtga tgctgtcgcc gaagaagtta
    12841 agaaaatcct tgcttaaaaa gattctcttt ttttatgata tttgtacata aactttataa
    12901 atgaaattca taatagaaac gacacgaaat tacaaaatgg aatatgttca tagggtagac
    12961 gaaactatat acgcaatcta catacattta tcaagaagga gaaaaaggag gatagtaaag
    13021 gaatacaggt aagcaaattg atactaatgg ctcaacgaaa tgacggtatc gataagcttg
    13081 atatcgaatt cctgcagccc gggggatcca ctagttctag agttaattaa
//
