LOCUS       sequence-208209-       13377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13377)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 13377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU70.2-2.2
FEATURES             Location/Qualifiers
     source          1..13377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2064..2363
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        4558..4778
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4779..5582
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(9524..9544)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9524..9542)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(9881..10469)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9961..9980)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10640..11431,11432..11500))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11263..11282
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11501..11605)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11632..12974
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(12595..12642)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     13015..13033
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(13352..13368)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13352..13368)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggattctg ctgcagatct tcgagggcga ggtagctgga
      541 gctatagcgc agccgaatat cctgtggtcg ctgtgacggc ctgctctgtt ctcctaattc
      601 tatgggcgat cgctcatatc ctttataaca ttttccttca tccgctcaga aatcaccctg
      661 gaccaaagct ctgggccgca actcgcgtac catggtgttg gtatcagtac caaggcatcc
      721 tacacgagcg tttattggag cttcatgtca agtacggtca cactgtccgt atcgctcctt
      781 acgaactctc cttcacctca gacacggcat ggaagacgat atatggacac agatccgtgg
      841 agatggacaa ggatcctgtt tttcgccttc acactcctac tggagcacag aatatcctgg
      901 tggccgaccg ccagacgcat attcgccaaa gacgactgct ttcccatgca ttttctgaaa
      961 aagcacttag ggagcaagaa ggtattttac aaacatatgt gagcaagctg ttggaacagc
     1021 tatctttgag atatactgca ggaccgctga acatggtcgc atggtacaat ttcacaacct
     1081 tcgacctgat aggagatctg tcgtctggcg aaaagttcgg gtgccttgac aagggtgatt
     1141 acgtcccctt cgtccgcgca acggagagga tagcaaaaga gctcaccttc acccaaatgc
     1201 taatctactt cggcgtggcg ggcgtccggc aattcttctt gcccaaagct gtggccggcc
     1261 agcgcgctca gaacatgaaa cgcgcaatcg acaccgtgga gcgacgaatc caacgggaca
     1321 ctgaccgaaa agacttcctc tactatatcc tcgccgccaa cgacgagaaa ggcatgagca
     1381 gggcagagat ccatgtaaat tcattttcct tcaacatcgc cggctcagaa tcaagtgcga
     1441 ctgctctttc cggaataaca taccacatcc tcacccgctc atccgcctac tcagaactca
     1501 tccacgaaat tcgcacctct ttcacgtccg aagaagagat cacaatcgcc agcaccaacg
     1561 acctcaaata cctcgaagcc gtcatttccg aatctctccg tctctacccg cccgtcgccg
     1621 gcaccctccc ccgtgtcgtg cccgccccag gcgagacgat tgatggcatc catgtaccca
     1681 ccggcaccac tgtcggtgtc caccacttct cttgcttccg ccacccggct aatttccacc
     1741 gcccagacga gtttctcccc gagcgctggc ttccggaatg caaagatgtt ccgccgtttg
     1801 acaaggacaa cagggcggcg gtgcagccgt tctcattcgg tccacggaat tgtttgggaa
     1861 agaatctagc acgagcggag atgaggctga taatggcgaa gctgctgtgg aggtttgatt
     1921 tagagttggt tccgggtggg ggagggagcg ggaagggaat gggaatggga aatggcaaga
     1981 gttggaggga tggacagaag gtctggggtt tttgggtcaa gccaccattg atgtgttggc
     2041 tgaggcctgt gagtagagat tgagcggatc tcttatgtct ttacgattta tagttttcat
     2101 tatcaagtat gcctatatta gtatatagca tctttagatg acagtgttcg aagtttcacg
     2161 aataaaagat aatattctac tttttgctcc caccgcgttt gctagcacga gtgaacacca
     2221 tccctcgcct gtgagttgta cccattcctc taaactgtag acatggtagc ttcagcagtg
     2281 ttcgttatgt acggcatcct ccaacaaaca gtcggttata gtttgtcctg ctcctctgaa
     2341 tcgtctccct cgatatttct cattttcctt cgcatgccag cattgaaatg atcgaagttc
     2401 aatgatgaaa cggtaattct tctgtcattt actcatctca tctcatcaag ttatataatt
     2461 ctatacggat gtaatttttc acttttcgtc ttgacgtcca ccctataatt tcaattattg
     2521 aaccctcacg atccagttct ccagtgacac agcctttatc tggtcaaacc tttctttcta
     2581 atcacctatg ctgatgctta attaagggat ttttgtctcc atcaacggca tgcgcccaaa
     2641 aatgacgttt tttttaaccc atagacacga aactacccat tttccaccgg cctgacctac
     2701 caccggaaca acggccatct ccaacttgca agttggggaa attaagagca tcgcaggttt
     2761 aatggaagaa aaaaaaaagg tacagcacag cgcaaatgga gttagttccc ttatgtcaca
     2821 cactcacaca cagtcggtca gatcaagcat actgggtgcg tataaataga gtggccattg
     2881 ccaccctgtt tatctcaaaa tctgtcttgt tagtggtctt ctcccttttt caggttacaa
     2941 ttctcttgtt tctacttagt atataagtat atcaagctat attaagcata ctatcaactg
     3001 tcaactctat cctcaaaata caatacaaaa tggccattca ctggtccctc gaccatctcg
     3061 agaagctcct ccccaagcga ctcttcgcta agctctcaga gatttcttgc aatcccaacg
     3121 ttcccataga agctggtggt aattacccaa tcatcaatgc cgagaccggg gatctgcttg
     3181 ccggcgtgcc gtacgccagg ggattccgcg ttcccaggag caaaatgcgc gctttgtgtg
     3241 ctgaaggaat tgatgtccag tacggcaaga accttacgga tcttgcgttc agcgagaagg
     3301 gtgaccttgt gttcgcctcg tttagcgacg gcacgatcgc ccaaggctct gtacttcttg
     3361 gcgccgatgg cccacgctcg aaagttcgag aattcgccat gtctggagca gaggcagcgc
     3421 aagtcagcaa gtttccgatc tgtcatcaca atatgactgt ttgctatcga gatgtggata
     3481 aggcaaagta tctgagacaa cggtttccaa cgagctattt ggctctgagc cagaagtcgc
     3541 accacgcttt tcagtcgatc tctagcatgc ctgatggtca agaccatcca gaaacatggt
     3601 tgtttcatct cgccatggcc tggctgggtg acccggagca ctcactcagc tacgaagagc
     3661 gtcttgccat cattaaggaa cgtgcacaag gtatgggcga gcctgctcga tcagcattca
     3721 cgtggatccc cgaggacacc ctggtccaca aggcagacat cagctattgg atcacgcaac
     3781 cgtgggacaa ccgtggaggt agagtcaccc ttgtaggaga tgctgcgcat cccatgccac
     3841 cctatcgtgg tcaaggcctc aaccactgca tctgtgacag ttctcacctc ctcgatggtc
     3901 ttgagctcgt acgtgccaac aaggcaacca tcaaggatgc cattactgcc tacgatgaag
     3961 aactcgtccc tcgcggacgt gacgaggtca aatgctcgtt ggagaacggc ctcatgctgc
     4021 atgactggca gaaggtgaag gagagccccg tattcaggaa cggattcagg cctatgactg
     4081 gtcacgacgg caaggaggct cttagtgagc atgcgcaggc gcagatgaag agggatgagg
     4141 aggagcagag ggatatccct gtcgctgcga cttgaacaaa tcgctcttaa atatatacct
     4201 aaagaacatt aaagctatat tataagcaaa gatacgtaaa ttttgcttat attattatac
     4261 acatatcata tttctatatt tttaagattt ggttatataa tgtacgtaat gcaaaggaaa
     4321 taaattttat acattattga acagcgtcca agtaactaca ttatgtgcac taatagttta
     4381 gcgtcgtgaa gactttattg tgtcgcgaaa agtaaaaatt ttaaaaatta gagcaccttg
     4441 aacttgcgaa aaaggttctc atcaactgtt taaaaggagg atatcaggtc ctatttctga
     4501 caaacaatat acaaatttag tttcaaagat gaatcagtgc gcgaaggaca taactcattc
     4561 aattcaattc atcatttttt ttttattctt ttttttgatt tcggtttctt tgaaattttt
     4621 ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga cttagattgg
     4681 tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc ttaacccaac
     4741 tgcacagaac aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct acatataagg
     4801 aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc atgcacgaaa
     4861 agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag
     4921 ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt
     4981 tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac
     5041 tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg
     5101 gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag
     5161 gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct agaggccttt
     5221 tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat actaagggta
     5281 ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca
     5341 tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg ggtttagatg
     5401 acaagggaga cgcattgggt caacagtata gaaccgtgga tgatgtggtc tctacaggat
     5461 ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct aaggtagagg
     5521 gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc cagcaaaact
     5581 aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt
     5641 aattatatca gttattaccc tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     5701 taccgcatca ggataggaaa aaaccgagct tcctttcatc cggcgcggct gtgttctaca
     5761 tatcactgaa gctccgggta ttttaagtta tacaagggaa agatgccggc tagactagca
     5821 agttttaggc tgcttaacat tatggatagg cggataaagg gcccaaacag gattgtaaag
     5881 cttagacgct tctggttgga caatggtacg tttgtgtatt aagtaaggct tggctgggga
     5941 tagcaacatt gggcagagta tagaagacca caaaaaaaag gtatataagg gcagagaagt
     6001 ctttgtaatg tgtgtaactt ctcttccatg tgtaatcagt atttctactt acttcttaaa
     6061 tatacagaag taagacagat aaccaacagc ctttcccaga tatacatata tatctttatt
     6121 tcagcttaaa caataattat atttgtttaa ctcaaaaata aaaaaaaaaa accaaactca
     6181 cgcaactaat tattccataa taaaataaca acatggctcc aagagtcgtc ttgatcacgg
     6241 gtgccaacac tggcattggt tttcagattg tgaggtcact ctgcagctct gatcaagctt
     6301 acgacatcat cgttggtggg cgatcgctgt ccaaggctga ggatgccatc agctccgctg
     6361 ttaaggagtt cccctcctct cgcagtaagc tttttccaat tcaggtcgac attgagcagg
     6421 atgactctat caaacacgcg ttcaacgagg tgcagtctaa gttcggaaag cttgatgctt
     6481 tggtaaacaa tgctggcgcg cagctcgaag cgcagtttgc cgctggtaag ctgacggagc
     6541 gcgacatttg gaaccagtca tggaatatca acaccgtggg ggcgcaagta atgacctcaa
     6601 gcttcatacc tctcctgctc cagactcctg acccgcgcct gctgttcatc acgtccaacg
     6661 cgtccactct cgctggaacc gagaaccttg ctctacccat tgaccgctct cctcctaaag
     6721 ggtggccgaa acgcggtttc agtattgcgg cataccgcag tgccaaaagc ggcatgaaca
     6781 tgatgatgcg tgaatggcac cgcacgctga aggaagacgg tgtgaaagta tggtgtcttg
     6841 ctccaggttt ctcggctacg ggccttggtg ggaatccgga ggcctcaaag aaaatgggag
     6901 ctggtgatcc ggcggtggcg ggccctttca tcaggagtgt tttggagggt gaaagggatg
     6961 gtgacgttgg taaggtcatt acgaaggatg gtgtgcaggc ttggtgaagt gcttttaact
     7021 aagaattatt agtcttttct gcttattttt tcatcatagt ttagaacact ttatattaac
     7081 gaatagttta tgaatctatt taggtttaaa aattgataca gttttataag ttactttttc
     7141 aaagactcgt gctgtctatt gcataatgca ctggaagggg aaaaaaaagg tgcacacgcg
     7201 tggctttttc ttgaatttgc agtttgaaaa ataactacat ggatgataag aaaacatgga
     7261 gtacagtcac tttgagaacc ttcaatcagc tggtaacgtc ttcgttaatt ggatactcaa
     7321 aaaagatgga tagcatgaat cacaagatgg aaggaaatgc gggccacgac cacagtgata
     7381 tgcatatggg agatggagat gatacctcca ttgggccgat gaagttagtc gacggataga
     7441 agcggttgtc ccctttcccg gcgagccggc agtcgggccg aggttcggat aaattttgta
     7501 ttgtgttttg attctgtcat gagtattact tatgttctct ttaggtaacc ccaggttaat
     7561 caatcacagt ttcataccgg ctagtattca aattatgact tttcttctgc agtgtcagcc
     7621 ttacgacgat tatctatgag ctttgaatat agtttgccgt gattcgtatc tttaattgga
     7681 taataaaatg cgaaggatcg atgaccctta ttattatttt tctacactgg ctaccgattt
     7741 aactcatctt cttgaaagta tataagtaac agtaaaatat accgtacttc tgctaatgtt
     7801 atttgtccct tatttttctt ttcttgtctt atgctatagt acctaagaat aacgactatt
     7861 gttttgaact aaacaaagta gtaaaagcac ataaaagaat taagaaaatg actacatttg
     7921 tagaatcatc catctcgccg cttttcgggc tgtggcagcc tcttccccac ttgaaacatc
     7981 tctattatgg ttcgtcttgc gttcaaaagc atctattcgc gactctccct tcacacgatt
     8041 cgaaagtttt catcgtcaca ggaaattcta tcgcgaccaa gactcctctt cttcaacaag
     8101 tcgagacttt actcggagat caccatgcgg gaacattcac caaaatcaga caacatgcgc
     8161 caatggccga tattgaccaa gccacggaga tcatcgccaa aaatccgttg gttgatacta
     8221 tcttatcgtt gggaggtgga tctcccatcg attccgcaaa aacaatatca ttccgcatgc
     8281 atgagagaaa tggcaaattc ctgacacatc tcacgatccc tacgacgctg agtgctgctg
     8341 agtgcacgat aggcggcggc tacacgaaag cagacggtgt caagacttca gtaatgacgc
     8401 ccgaaattgg tgtgactgca gtattttacg acccgggcta tgccaaatat actccgttga
     8461 agctatggct atcgactggt atgcgtgcta tggaccacgc cgtggaatca caatatcagc
     8521 catacgcgtc agagatgcca tggagggcaa tgtcgtcttg ggcagtgtcc accttctttg
     8581 aatgtctacc aatagccaag gcatctcatc ccagcgacga agacgttata acgagattac
     8641 tacttgctgc gtttgccagc ttgggcttcc gcgatggaga gactagctgc ctcactctcg
     8701 gtcccgtggt ccagcttaag gcgcgggcca agcaagagga cgcaaaacaa attgcaagac
     8761 tcctgccttg gattggcggg acgtcgagcg gtgatgatct caaagatgct ttggaggtcg
     8821 gatgcaagat cagtgcgtta gtagagagcc tgggattaca tcaaagcctt acagagaggg
     8881 gtattggcag agaccagatt ccaatcgtgg tggaacgggc tactggtggt ttgaaagaag
     8941 gctcactata tgacatcgtc acgcttttgg tcgagggttt atactaagga gattgataag
     9001 acttttctag ttgcatatct tttatattta aatcttatct attagttaat tttttgtaat
     9061 ttatccttat atatagtctg gttattctaa aatatcattt cagtatctaa aaattcccct
     9121 cttttttcag ttatatctta acaggcgaca gtccaaatgt tgatttatcc cagtccgatt
     9181 catcagggtt gtgaagcatt ttgtcaatgg tcgaaatcac atcagtaata gtgcctctta
     9241 cttgcctcat agaatttctt tctcttaacg tcaccgtttg gtcttttata gtttcgaaat
     9301 ctatggtgat accaaatggt gttcccaatt catcgttacg ggcgtatttt ttaccaattg
     9361 aagtattgga atcgtcaatt ttaaagtata tctctctttt acgtaaagcc tgcgagatcc
     9421 tcttaagtat agcggggaag ccatcgttat tcgatattgt cgtaacaaat actttgatcg
     9481 gcgctatgcg gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa
     9541 ttgcgcgctt ggcgagactc aaacaagata tattttgccc gccttacaga cgaaactagt
     9601 tggaggttat ggagcatact atcacgtggg cggccactgg tgagttacta caccccaggg
     9661 gcaacgttga tgctcctaaa aaactctggc tggacgcaag ccgtaacacc cgtgtcactt
     9721 cataatcgtt tgcaattcag ggcttgatct acacattacc ctgttatccc tattaattaa
     9781 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     9841 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     9901 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     9961 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    10021 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    10081 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    10141 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    10201 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    10261 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    10321 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    10381 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    10441 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    10501 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    10561 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    10621 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    10681 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    10741 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    10801 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    10861 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    10921 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    10981 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    11041 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    11101 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    11161 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    11221 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    11281 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    11341 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    11401 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    11461 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    11521 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    11581 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gaacgaagca
    11641 tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag
    11701 aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt ttaccaacga
    11761 agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa
    11821 caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg ctattttacc
    11881 aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag cgctattttt
    11941 ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat gcagtctctt
    12001 gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg gtgtctattt
    12061 tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact agcgaagctg
    12121 cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga tgtggattgc
    12181 gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca gaaaattatg
    12241 aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac attttcgtat
    12301 tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa agagtaatac
    12361 tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag gagcgaaagg
    12421 tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag atacttttga
    12481 gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt ccggtgcgtt
    12541 tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt
    12601 cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg
    12661 agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca cgtcgcacct
    12721 atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt gcgtgtttat
    12781 gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc tagtacctcc
    12841 tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact accctttagc
    12901 tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc tatcatttcc
    12961 tttgatattg gatcatacta agaaaccatt attatcatga cattaaccta taaaaatagg
    13021 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctcggcgcg
    13081 ccattaccct gttatcccta atatacaacg caaagaagct ggaaaattgg cgagggaatc
    13141 ctgtttctgt ctatccaaga atgggcatga ggtggcaacc gtcgtgctag cgtacagggt
    13201 gcactttgta accatttggg acaccggaca ctcgctgttt tcgaaattac cctttaagcg
    13261 cgggtattga accaggctta tgcccaagat cgtagcaagc atttaaatga cggtatcgat
    13321 aagcttgata tcgaattcct gcagcccggg ggatccacta gttctagagt taattaa
//
