LOCUS       sequence-208210-       13613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13613)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 13613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU71.1-2.2
FEATURES             Location/Qualifiers
     source          1..13613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             504..527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(6817..6828)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8048..8068)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8048..8066)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(8405..8993)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8485..8504)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9164..9955,9956..10024))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9787..9806
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10025..10129)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10156..11498
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(11119..11166)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     11539..11557
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11829..12233
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             12246..13340
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(13588..13604)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13588..13604)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggattata aggatgatga tgataagact agtatgatgc
      541 agcgacatcc taccgccgag gccacaaccg cgggtggctg cgtgcatcac ctctttgaac
      601 atgctgttaa gggctaccca gacaacattg ctctgatcgg tggcacgcat gagctatcct
      661 tccagcagct taacacgctc gctaaccatt ttgccagtgc tctggtcagg cgaggagtag
      721 ggcgcggcga tctggtcgct atagcgctgg atcgatctgt ggatctggtc gtcgccttgt
      781 taggagtcct taagtcgggg gcggcatacg tgcccgtaga tatgggtttc cctattcggc
      841 ggatcaggca gatgatggat gatgcgggcc cgaagtttgt tatatgtcga gatagtacac
      901 tggatgcact gtcatcctgg agagatatat gcctgagtgt tgatgacata cgaaacaaca
      961 acggcgagaa ccactccttg tccggccctg atggaggcaa cttggacctg gacgtgtgcg
     1021 ccgaggatct cgcatatatc atctatacat caggttcgac tggaaagccc aagggcgtag
     1081 aaatcagcca tggagcggta tcaaatcttc tctcgtcgat gcgacgtgaa ccgggatgcg
     1141 accaaacgga tcgactacta gccataacaa ccatttcttt cgatatagcc gttctagagc
     1201 tttttctgcc actcgtctgc ggcgcaaaga cggtcattgc ccaggcgcat caagtccgcg
     1261 acgcgggagc tctaatcaaa ttattgaaac accacgcaat caccatgatg caaggaaccc
     1321 cagccacctg gcaattgttg ctggattcgg gctggcaagg caacccccgg ttaggaaaag
     1381 ttttatgtgg tggcgaggcg ctttctcgtc gactggccga caggctgctt tcttgcgccg
     1441 actcggtatg gaacatgtac gggccaaccg aagccactgt gtgggcgagt gtttggcggg
     1501 tgggccttgg tggtgatgtg ctcattggga ggccaataga taacatgcaa ttctacatac
     1561 tcggcgaagg cctatcgcca gtgccgcaag ggagtcctgg tgagctgtac attggaggtg
     1621 ctggcgtcgc ccgcggctat catcgcaacc ctgagcttac tcgatctcgc ttcatcgaga
     1681 gccctcgtca tggcggcatt ctgtaccaaa caggggactt agcccgttgt ctggcgcccg
     1741 acgagctcgt ggtgctgggc cgggtggatg accaggtaaa gatccggggc catcggattg
     1801 agctaggcga tatcgaagcg gcacttgctg cccacgagga tgtctccaag gccgtggtcg
     1861 tcagtcgtgc cgaccaactg gtcgcctact gtgtccggaa actgcctcag gtcactgatg
     1921 aggaccaggc ccgcagcctg gcagagtgga ctgccacgtg ggaccatgcg tacgaaacca
     1981 cagatcaaga cgccgtgagc caggatgcca tcctcaacct ggccgggtgg aggaatagct
     2041 acgacggcct cccgttcagc accaatgaaa tgcgtgactg gcagtcaagt tccgtcagac
     2101 gcatcttgtc ctacggcccg gaacgagtca tggagattgg ctcgggcacc gggatgatgc
     2161 ttttcagtat cgcaccgcac tctagtatct actacgctat cgattcttcg agaaaagcag
     2221 tcgagtttgt gcgccgtcac ctggggttgt tgccccatgt cacgtgcgag catcgtgaag
     2281 ctcacgcact gcccgtgata gagcaggagt tcgacacggt catcgtcaat tctgtcatcc
     2341 agtatttccc cagtatcgac tatctagtct tgttcctcga atgggcggtt caggcagtcg
     2401 gcaaaggccg tatatatctt ggcgacgtgc gcaacctggc gctgctaaac gtcttccatg
     2461 gcgatatatt gcattcccgc ataaagggcc aaatccccgc cgttgactta gctagccggg
     2521 ccgcagaggc ggcaaaagcc gatcgtgaat tggtactttc ccctgatttc ttcgcctctt
     2581 tggccaatgt cttcccgcgg atatctcatg ttgatatcac gttgcgcgat ggtgattacg
     2641 ataatgaaat gaccctctat cgctttgatg tcaccttgca tattggagag cgtgacgaga
     2701 ttggtacgag gccgagagag gtggattggc ggactaaggt cgatttgtcc gctctaaagg
     2761 atgagctaat ggatgctgcg aaaggcaaca atatgctgcg tttaaacaat gtccctaatg
     2821 ggaggctgcg taatgtccac agtcgactcg ggactattct cggctacccg tcatacgata
     2881 gctcgtcctg ggtccatccg cgtattctcc atgctgtcgc tcgagatgtt ggtatgcagt
     2941 cggcactttt gcccagccgg tcaaacgatg tttggagttt cgacgcggtg ttttggcggg
     3001 ctggccagac cccagatctc acactgcaac cctccaaagc aatggacaag agcgcatggg
     3061 ctgaatacgc gaatgtacct aacatcggaa aagcgatcag tccggctacc ctcgacagag
     3121 tgctccgacc atggctagca gaccggttac cgacatatat gatgccatcg tttttcgtgg
     3181 tactcgacgc cttgccactg acacccaatg gcaagatcga ccgcaagagg ctaccagatc
     3241 cattagtaag agacagggca attcaaaagc cagccacgca actagaggcc gatatactgg
     3301 ccgtttggtc gcgggtgctg ggccagaatg gtatcgatgt caaagataac ttcttccagg
     3361 tcggagggga ctcgttacgt gtgactaggg tgcaaaccga gttggagcaa ttgctcaggc
     3421 ggccgatctt gctcgccaaa ctattcgaac attacaccgt caagacactc gcggcatact
     3481 tagctgaaga tatgagcaat gatgtgaaaa cagacccgaa agaccatgtt tctgttagcc
     3541 acaatgacga agacattgct gtagtatcta tggcatgccg gctccctggt ggagtcacca
     3601 cccctgaaga gttttggaag ctgcttgaaa gcggaggtga tgcaatcagc gaagtaccca
     3661 aggctcggtg ggatgcagag gctatctata atgcggatcc agacgcaaag ggaacatcgt
     3721 actgtcgtca aggtgggttt ctcgacaccc cgattgactg ctatgataca gggttttttg
     3781 gaatttcgcc ccgggaagca cggtcgttgg atccaatgca atacatgatg ttagagacgt
     3841 gctgggaatc gtttgagcga gctgggtata caagcgaaca gctccgtggc agccagacag
     3901 gagtatacgt aggggtcagt aatattccgg catactcaaa ccctaacggc agcggaaaag
     3961 ctctagaaga cctagacgga tatgcaggga cggggtcggc tgccgcgacc atgtctgggc
     4021 gtgtttcgta cacgtttggg ctcgaaggtc cgagcatgac ggtggacacg gcgtgctcat
     4081 cttcgttagt cacgacccat ctagcatgta ctgccttgcg ccaaggtgag tgtgacatgg
     4141 cagtgtctgc tggggttagt ctgatgctct cgccgggggt gcatgttgaa tttagtcgac
     4201 tcaggggaat ggcgagagac ggacgatgtc gagctttcgc ggccgacact gaaggcacag
     4261 gatggagcga ggggtctgtg gtaatggttc tcaagcgcct atctgacgcc cagcgagatg
     4321 gtgatcgcat cctggcggtg gtgcgtggct cagcagttaa tcatggcgga cgcagcagcg
     4381 cctccctcac gacgccgagc ggaccggctc agaaacggct cattcggact gcgctggccg
     4441 cgtctaggtt gcaggcgagc gacatcgatt acatagaagc tcatgggaca ggcactaagc
     4501 ttggtgaccc catcgaaggg gtggcactgg cagaggtttt caaggacggt acccgtgagg
     4561 agccgttgtg ggttgggtca gccaagtcca acatcggtca tacccaagca gccgcgggtt
     4621 tggctgggat gttgaaggtg gtgctggcca tgcagcacgc cattctaccg cagacgttgc
     4681 atgtatcgaa caacccgacg ccagcagtgg agtgggagag agcaaacatg gcactggtgc
     4741 aggagaagcg gtcgtggcgg cgacgaaccg cgcggcgaaa ggcaggtatc agcgcctttg
     4801 gtatcggtgg caccaatgcc catgtaatcc ttgaggagtg gccgtcgctt gcaccagcgg
     4861 tggccaatct tgatcctcgt tgtgatcctc acggtaaaga tggtggtatt gctacacctc
     4921 ctttgaaacc gctgctgctc ctgtcgggcc acagcaatgc tgcgctgcga gcacaggcca
     4981 aaaaactttt atcccatttg actaataaca gtacggcgca ttattctctg tacgatgtgg
     5041 cctactcact ggcaactaca cgcaatcatt tccgaagacg aaaggtgctg attgcgcagg
     5101 ataagaggga tctggcaagc cagctggcaa ccattgctgc cgcagagtcc actaacgcgc
     5161 cagcagagga tatggtgcag ccatccagag gccaggtggc catgatattc tccggtcagg
     5221 gtagccaatt tctggatatg ggcagagatc ttgcacaaca cttccctgtt tttcgcgacg
     5281 ccttggagga gattactgcg cactttcatg gatatttaga gcggccactg ttggatgtca
     5341 tgtggagtgg tgatgctgac agcaggaatg atgccctgct gcaaaggact gactacgccc
     5401 aacccgccct gttttcttta gaggtggcgc tgtggcgtct gtggaccagc tggggcgtga
     5461 ggccagacgt gctgctaggc cacagcattg gtgaactggc agtagctcac gtcgccggcg
     5521 tgctaaacct gagtgatgct gcccgtttgg ttgctgctcg tggtcggttg atgcaggcag
     5581 tagatactcc tggggagatg gtatcgctag aagccagtgc ttccgaagtg tctgcagcca
     5641 ttgacggttt agggcttggt atgaaggtcg atattgcctg tcacaatgcc ccctcacaaa
     5701 ccgtcgcctc tggtgatata gatgccgtca aagggctacg cacccacttt gccactcggg
     5761 gccgtaaatt ccattctcta agcgtctccc atgccttcca ttctcaccat atggaggcaa
     5821 tactggcgga ttaccgcgcc gtagcagaga cagttgattt cagccctcct aatataccca
     5881 tcgtcagcag tctaacgggt cagagagtgg ctcctggcca gctagagcag cctgcatact
     5941 gggtacagca ggcgcgcaac gctgtccgtt tcagtgatgg aatacaaacc cttctgatgg
     6001 atcacgatat caatatcttc ttggaggtag ggccgcaaac ggttttgtgc ggcatgggtg
     6061 ccgcctgtgt cgatgatatt cgccattcca ccattgcgcc tacctggttg ccgtcaatta
     6121 ttcgggaccg ggacgctagc tccgttattc aacagagtct tgccgaaata cacattcaac
     6181 atgtgtctat cgattggtcg gcttacttta aagctgccag ctgtcaacgt gtcgacctgc
     6241 cgacctacgc ttttcaacgg gagcctgtgt acactataaa cagggacctc aaattcaagc
     6301 gtagccccac ttctaaccag gtgactagcg tcgcaacagc tgctgctctt catggaacag
     6361 atcctctcga tgggctgcgc agctccttga tcgtggctgc tccggcagag caactgagaa
     6421 tcgtattatc catgattcga gaaactgtgg ccaagatctt ggaccatttg tcgcccgacg
     6481 atgtcgacct cgacctcccc ttgcaagacc tgggaattga ttcactcact gcattgctga
     6541 tgcggaacca gcttacagcg cagactggtt tgaatttccc ggctatggtg acactgcatc
     6601 atcccaactt aagaagtctg ggtcagttcc tcttacacca ggcattacag gaaacgtcgt
     6661 cagcttcatc acctagcagc ccaaaatctt ctatagcaac gcctgcttcc tcaggttcac
     6721 cacagttgtc accgacagcg gcacaagcga tcaatggtca ttctgaaccg ttgcagcaca
     6781 ccaaaatgcc ttctatcgcc ccccttcatc gcgtcttctc ccctctattg ggcttgaatc
     6841 ctccaaatac gcctccacac ctgttctcgt ctgccatgag ctacttcagc aattttgatt
     6901 ggtgctcccg ccttatttac gattcctcgc ctacctcatc atctagtgtg ctaccaggtt
     6961 acggacaggc tattcccttt atcccacaat gcttcaatcc cgctagtccg cggcatgatc
     7021 aatttatcgg caactggctt tcaaaccaca agaaaacgat cggtagcgac aatgatacaa
     7081 aacaaagtcc tcctcttcgc cacatgctct ccctcttccg accccgcgac ccttatcaca
     7141 ttgaagatcc cttgcgccat atccctcgtg ttgcttccct gtttgctcta ggggagggaa
     7201 cgtcgggata cgagggcgtt atacatgggg gctttacagc tacccttctc gatgagagca
     7261 tcagcatagt tcatgagatc aataccgcgc taggcaagac gggcaaagtc ttcgccgcca
     7321 tcaatgtcac ggcttctctc agcgtcaagt tctttgctcc ggtagccgcg accgaagaag
     7381 tagtatgtgt gacagcgtgg atagaaggca tacaagggaa caatacaaca atgaaggcgg
     7441 agctcacaga tctcgcagga aataagctag ctgaagcaga gagcgtgttt gttgccgtga
     7501 atcaaaagtg aggagattga taagactttt ctagttgcat atcttttata tttaaatctt
     7561 atctattagt taattttttg taatttatcc ttatatatag tctggttatt ctaaaatatc
     7621 atttcagtat ctaaaaattc ccctcttttt tcagttatat cttaacaggc gacagtccaa
     7681 atgttgattt atcccagtcc gattcatcag ggttgtgaag cattttgtca atggtcgaaa
     7741 tcacatcagt aatagtgcct cttacttgcc tcatagaatt tctttctctt aacgtcaccg
     7801 tttggtcttt tatagtttcg aaatctatgg tgataccaaa tggtgttccc aattcatcgt
     7861 tacgggcgta ttttttacca attgaagtat tggaatcgtc aattttaaag tatatctctc
     7921 ttttacgtaa agcctgcgag atcctcttaa gtatagcggg gaagccatcg ttattcgata
     7981 ttgtcgtaac aaatactttg atcggcgcta tgcggccgcc accgcggtgg agctccagct
     8041 tttgttccct ttagtgaggg ttaattgcgc gcttggcgag actcaaacaa gatatatttt
     8101 gcccgcctta cagacgaaac tagttggagg ttatggagca tactatcacg tgggcggcca
     8161 ctggtgagtt actacacccc aggggcaacg ttgatgctcc taaaaaactc tggctggacg
     8221 caagccgtaa cacccgtgtc acttcataat cgtttgcaat tcagggcttg atctacacat
     8281 taccctgtta tccctattaa ttaatacggt tatccacaga atcaggggat aacgcaggaa
     8341 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8401 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8461 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8521 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8581 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8641 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8701 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8761 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8821 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     8881 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8941 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     9001 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     9061 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     9121 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     9181 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     9241 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9301 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9361 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9421 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9481 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9541 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9601 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9661 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9721 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9781 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9841 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9901 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9961 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    10021 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    10081 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    10141 gaaaagtgcc acctgaacga agcatctgtg cttcattttg tagaacaaaa atgcaacgcg
    10201 agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac
    10261 gcgaaagcgc tattttacca acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca
    10321 acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat
    10381 gcaacgcgag agcgctattt taccaacaaa gaatctatac ttcttttttg ttctacaaaa
    10441 atgcatcccg agagcgctat ttttctaaca aagcatctta gattactttt tttctccttt
    10501 gtgcgctcta taatgcagtc tcttgataac tttttgcact gtaggtccgt taaggttaga
    10561 agaaggctac tttggtgtct attttctctt ccataaaaaa agcctgactc cacttcccgc
    10621 gtttactgat tactagcgaa gctgcgggtg cattttttca agataaaggc atccccgatt
    10681 atattctata ccgatgtgga ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga
    10741 ttcttcattg gtcagaaaat tatgaacggt ttcttctatt ttgtctctat atactacgta
    10801 taggaaatgt ttacattttc gtattgtttt cgattcactc tatgaatagt tcttactaca
    10861 atttttttgt ctaaagagta atactagaga taaacataaa aaatgtagag gtcgagttta
    10921 gatgcaagtt caaggagcga aaggtggatg ggtaggttat atagggatat agcacagaga
    10981 tatatagcaa agagatactt ttgagcaatg tttgtggaag cggtattcgc aatattttag
    11041 tagctcgtta cagtccggtg cgtttttggt tttttgaaag tgcgtcttca gagcgctttt
    11101 ggttttcaaa agcgctctga agttcctata ctttctagag aataggaact tcggaatagg
    11161 aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata
    11221 cagctcactg ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag
    11281 aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct atttatgtag
    11341 gatgaaaggt agtctagtac ctcctgtgat attatcccat tccatgcggg gtatcgtatg
    11401 cttccttcag cactaccctt tagctgttct atatgctgcc actcctcaat tggattagtc
    11461 tcatccttca atgctatcat ttcctttgat attggatcat actaagaaac cattattatc
    11521 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
    11581 gatgacggtg aaaacctcgg cgcgccatta ccctgttatc cctaatatac aacgcaaaga
    11641 agctggaaaa ttggcgaggg aatcctgttt ctgtctatcc aagaatgggc atgaggtggc
    11701 aaccgtcgtg ctagcgtaca gggtgcactt tgtaaccatt tgggacaccg gacactcgct
    11761 gttttcgaaa ttacccttta agcgcgggta ttgaaccagg cttatgccca agatcgtagc
    11821 aagcatttaa ctgtgggaat actcaggtat cgtaagatgc aagagttcga atctcttagc
    11881 aaccattatt tttttcctca acataacgag aacacacagg ggcgctatcg cacagaatca
    11941 aattcgatga ctggaaattt tttgttaatt tcagaggtcg cctgacgcat ataccttttt
    12001 caactgaaaa attgggagaa aaaggaaagg tgagaggccg gaaccggctt ttcatataga
    12061 atagagaagc gttcatgact aaatgcttgc atcacaatac ttgaagttga caatattatt
    12121 taaggaccta ttgttttttc caataggtgg ttagcaatcg tcttactttc taacttttct
    12181 taccttttac atttcagcaa tatatatata tatttcaagg atataccatt ctaatgtctg
    12241 cccctatgtc tgcccctaag aagatcgtcg ttttgccagg tgaccacgtt ggtcaagaaa
    12301 tcacagccga agccattaag gttcttaaag ctatttctga tgttcgttcc aatgtcaagt
    12361 tcgatttcga aaatcattta attggtggtg ctgctatcga tgctacaggt gtcccacttc
    12421 cagatgaggc gctggaagcc tccaagaagg ttgatgccgt tttgttaggt gctgtggctg
    12481 gtcctaaatg gggtaccggt agtgttagac ctgaacaagg tttactaaaa atccgtaaag
    12541 aacttcaatt gtacgccaac ttaagaccat gtaactttgc atccgactct cttttagact
    12601 tatctccaat caagccacaa tttgctaaag gtactgactt cgttgttgtc agagaattag
    12661 tgggaggtat ttactttggt aagagaaagg aagacgatgg tgatggtgtc gcttgggata
    12721 gtgaacaata caccgttcca gaagtgcaaa gaatcacaag aatggccgct ttcatggccc
    12781 tacaacatga gccaccattg cctatttggt ccttggataa agctaatctt ttggcctctt
    12841 caagattatg gagaaaaact gtggaggaaa ccatcaagaa cgaattccct acattgaagg
    12901 ttcaacatca attgattgat tctgccgcca tgatcctagt taagaaccca acccacctaa
    12961 atggtattat aatcaccagc aacatgtttg gtgatatcat ctccgatgaa gcctccgtta
    13021 tcccaggttc cttgggtttg ttgccatctg cgtccttggc ctctttgcca gacaagaaca
    13081 ccgcatttgg tttgtacgaa ccatgccacg gttctgctcc agatttgcca aagaataagg
    13141 ttgaccctat cgccactatc ttgtctgctg caatgatgtt gaaattgtca ttgaacttgc
    13201 ctgaagaagg taaggccatt gaagatgcag ttaaaaaggt tttggatgca ggtatcagaa
    13261 ctggtgattt aggtggttcc aacagtacca ccgaagtcgg tgatgctgtc gccgaagaag
    13321 ttaagaaaat ccttgcttaa aaagattctc tttttttatg atatttgtac ataaacttta
    13381 taaatgaaat tcataataga aacgacacga aattacaaaa tggaatatgt tcatagggta
    13441 gacgaaacta tatacgcaat ctacatacat ttatcaagaa ggagaaaaag gaggatagta
    13501 aaggaataca ggtaagcaaa ttgatactaa tggctcaacg aaatgacggt atcgataagc
    13561 ttgatatcga attcctgcag cccgggggat ccactagttc tagagttaat taa
//
