LOCUS       sequence-208213-       12910 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12910)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 12910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU72.2-2.2
FEATURES             Location/Qualifiers
     source          1..12910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2148..2447
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        5275..5495
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             5496..6299
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(9057..9077)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9057..9075)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(9414..10002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9494..9513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10173..10964,10965..11033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10796..10815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11034..11138)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11165..12507
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(12128..12175)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     12548..12566
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(12885..12901)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12885..12901)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atgcgtcgtt cttcactagc ctaccgtcgt gtcctccccg
      541 gtgcacccac ctctcgatct gctgcctcct acgctatcgg ctttgaaaac gctcaagagc
      601 agcgtgtccc cgtcgacctt accatccatg gcacgattcc gtcctggctt agtggtgtct
      661 tatatcggac aggaccagga acctaccgga ttccctcaac caataaccca tcaggatccg
      721 ttgacattca acactggttt gacggactca gcatgaatca tcgcttcgag atccatcccg
      781 gtggaagtcg agtatcttat cactctagga aggcctctga ggactacgag aaacagatca
      841 tagaacacgg caaagtcccc ggcattgcct ttggtcaacg accagacatt tgccagagca
      901 tctttcgcaa gttcttcacg gtctttcagc agatccgctc tccaccacaa cccagtactt
      961 ctccgtccgg actcaatgtg caagtcacct tgacgccaga gatgcctgga tggaagagct
     1021 tgctccaact tgatcaccgc cacacttctg gtccacagta cattgtcgcc aagaccgatg
     1081 cagatggcct tcaggtcatt gatcctgtct ccctcgagcc actcgctaca gggacctata
     1141 agacattgga tccacgactc gatggtcagc tcagcgccgc gcattcatgc cgcgacaagg
     1201 agactaacga gttcttcaat tactcctgca aggttggcgg tcgttttgct acgtacaagg
     1261 tgtttagaat caatggtgat gatatagtgg acgtccttgc tgagatcaaa gacgcgcctg
     1321 cttcatacct tcactccttt gctatgacct cgaagtacgt cgtccttgcg gtctggcaag
     1381 ctcatatcac tgggtatggg ctgtcgatac tctacaaccg gaatatcgca gagtccattg
     1441 acaagaaatg gaatcccaat ttaaagagcg tcttctacgt cattgataag aggaatggag
     1501 gcattgtagc gaaatatgag actcccccgt tcttctgttt ccatcaactc aatgcctacg
     1561 acgatcctgc gaaggacgat atcgtagtgg atatgtcagt ctacgatgac aacacggtaa
     1621 tagaccgcct ctacctcgat aagcttcgca atctcacagc cgagaacccc atgctcatgg
     1681 gacgtgctcg ccgttttcgc cttaatgccg tttcctcttc atccaagtcc acaagaccag
     1741 ccatcgtcga cttcaccttt tcgcaatctg aatcaatcga acttcccact attaacccat
     1801 tcagctacca caagccttac cgttacgcgt atggaatcaa caagctggat cctcgggccc
     1861 atcatacgtt cgcagatagg atcatcaagc tcgacatgca gaacatcaat cacggacata
     1921 aggtctgggg tatcccaggc tataccccga gcgaaccgat attcgttcca cgtcccgggt
     1981 cagaagctga ggacgacggt gtccttctta gcgtggttct cgatggagag cgcggaagga
     2041 gcatgttgat tgtactgaat gcagaggata tgactgaatg tgctagggcc gagatggaga
     2101 caacttttcc cattggattc catggcattt ggacttcgac ggagtgagcg gatctcttat
     2161 gtctttacga tttatagttt tcattatcaa gtatgcctat attagtatat agcatcttta
     2221 gatgacagtg ttcgaagttt cacgaataaa agataatatt ctactttttg ctcccaccgc
     2281 gtttgctagc acgagtgaac accatccctc gcctgtgagt tgtacccatt cctctaaact
     2341 gtagacatgg tagcttcagc agtgttcgtt atgtacggca tcctccaaca aacagtcggt
     2401 tatagtttgt cctgctcctc tgaatcgtct ccctcgatat ttctcatttt ccttcgcatg
     2461 ccagcattga aatgatcgaa gttcaatgat gaaacggtaa ttcttctgtc atttactcat
     2521 ctcatctcat caagttatat aattctatac ggatgtaatt tttcactttt cgtcttgacg
     2581 tccaccctat aatttcaatt attgaaccct cacgatccag ttctccagtg acacagcctt
     2641 tatctggtca aacctttctt tctaatcacc tatgctgatg cttaattaag ggatttttgt
     2701 ctccatcaac ggcatgcgcc caaaaatgac gtttttttta acccatagac acgaaactac
     2761 ccattttcca ccggcctgac ctaccaccgg aacaacggcc atctccaact tgcaagttgg
     2821 ggaaattaag agcatcgcag gtttaatgga agaaaaaaaa aaggtacagc acagcgcaaa
     2881 tggagttagt tcccttatgt cacacactca cacacagtcg gtcagatcaa gcatactggg
     2941 tgcgtataaa tagagtggcc attgccaccc tgtttatctc aaaatctgtc ttgttagtgg
     3001 tcttctccct ttttcaggtt acaattctct tgtttctact tagtatataa gtatatcaag
     3061 ctatattaag catactatca actgtcaact ctatcctcaa aatacaatac aaaatggcat
     3121 ccaactggaa accaaaacgc tcgtttgaag aagtggagaa actgttatgt gcaccaggaa
     3181 cactgttgga aatggaacag aggttgattg ctggccaagt tcagcgcgtc tacaaaaact
     3241 tatggccatc tgtccgacta ttctttctct ccgtcaccgc cctacatgcg gacaagatca
     3301 atgtggtgtt tgagaacgaa cgtctgactt ttcaggagat gaatgagcgt gccactcgag
     3361 cggcagcgat tcttcgcgat gtctatggtg tattgaaggg tgaccgtgta gccatttgct
     3421 ctcgcaactt tcctgaatac ctggtcgtct tttgggctat ccatctcctt ggagctgtac
     3481 cagtattagt gaacgcttgg ttaccaagcg aacctctaga gcattgtctg acgcgggcgc
     3541 ggcccaaact catattcttg gatccagaaa gagcaaagat cgtgcaacct atggtgccaa
     3601 aattgtccaa aataggcgtg aaagcatata tcgttttgca aagtcacgaa gggaaaggac
     3661 agtggccagg catgcagact tgggaggccg ttacgaagag ctatagggga gacgcttcaa
     3721 ccgttctgac ccaagacccc ggtatcctcc ccgaagataa ctgcattatt atcttcacct
     3781 cagggtcgac gggacttcca aagggcgtcc ttttgagtca gcgggcgttt gttactgcta
     3841 tcggcaatgc cctcgctgga cgaggtcgtg ccttactccg taagggagaa tcattccctc
     3901 cgcccccggc agaaggtcct cagccgggat tgttactacc aacaccgttc ttccatgtta
     3961 ccggtacaac cttgacttta tttgctgctg tcggcggagc caaactcgtt ctaatgcgca
     4021 agtgggatgt gaaagaagcc gtcagactat tcaaagcgga gaacatcaca gctgctggcg
     4081 gtgtaccctc aacagtagtt gacttgctcg acaacggagg atctggacat ccactagatg
     4141 gcgtgatgct tggaggtgca gccgtctcgt cgacgatagc tgcgcgagcc aaggcagcgt
     4201 tccctggttc cacattatca caggcttatg gtcagacaga gtgcaatgcg actgctgttg
     4261 gattctctgg agaggactat gagacccggc tcagttcatg cggacgcgct atgcctgtca
     4321 acgatctgtt aatcatgaaa gacgatgtag agtgcaagct gggtgaacca ggtgaagtct
     4381 ggattcgggg accgaacgtt atgaaggaat attggggcga tccagtcgcc acagcaaagg
     4441 tcctcacgaa agatggctgg gtgaaaactg gtgacatcgg atacatggat gaagaagggt
     4501 tcctttacat caaagaccgg ttgaaagata tcatcattcg tggaggtgaa aatgtcgact
     4561 ctgtgatggt tgagaacgct ttatataggg atcctcggat ctttgaagca gctgctgtcg
     4621 gggtccctga cagacgacta ggcgaattgg tcgctgccct cgtcacgttg aaatctgcgt
     4681 atagaggcaa aggacaggtt acagaagccg agctgctggc tttagctcgg aaacatttac
     4741 ctaggttcgc agtacccgtg atgattttga ttcgggaagg agattttgag cacaccccgt
     4801 ctgagaagat catcaagatg caactacgaa agattgccgc tgaagagtgg gagaaacgca
     4861 aggcttctga aaacggacag gccaaattgt agacaaatcg ctcttaaata tatacctaaa
     4921 gaacattaaa gctatattat aagcaaagat acgtaaattt tgcttatatt attatacaca
     4981 tatcatattt ctatattttt aagatttggt tatataatgt acgtaatgca aaggaaataa
     5041 attttataca ttattgaaca gcgtccaagt aactacatta tgtgcactaa tagtttagcg
     5101 tcgtgaagac tttattgtgt cgcgaaaagt aaaaatttta aaaattagag caccttgaac
     5161 ttgcgaaaaa ggttctcatc aactgtttaa aaggaggata tcaggtccta tttctgacaa
     5221 acaatataca aatttagttt caaagatgaa tcagtgcgcg aaggacataa ctcattcaat
     5281 tcaattcatc attttttttt tattcttttt tttgatttcg gtttctttga aatttttttg
     5341 attcggtaat ctccgaacag aaggaagaac gaaggaagga gcacagactt agattggtat
     5401 atatacgcat atgtagtgtt gaagaaacat gaaattgccc agtattctta acccaactgc
     5461 acagaacaaa aacctgcagg aaacgaagat aaatcatgtc gaaagctaca tataaggaac
     5521 gtgctgctac tcatcctagt cctgttgctg ccaagctatt taatatcatg cacgaaaagc
     5581 aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa ggaattactg gagttagttg
     5641 aagcattagg tcccaaaatt tgtttactaa aaacacatgt ggatatcttg actgattttt
     5701 ccatggaggg cacagttaag ccgctaaagg cattatccgc caagtacaat tttttactct
     5761 tcgaagacag aaaatttgct gacattggta atacagtcaa attgcagtac tctgcgggtg
     5821 tatacagaat agcagaatgg gcagacatta cgaatgcaca cggtgtggtg ggcccaggta
     5881 ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa ggaacctaga ggccttttga
     5941 tgttagcaga attgtcatgc aagggctccc tatctactgg agaatatact aagggtactg
     6001 ttgacattgc gaagagcgac aaagattttg ttatcggctt tattgctcaa agagacatgg
     6061 gtggaagaga tgaaggttac gattggttga ttatgacacc cggtgtgggt ttagatgaca
     6121 agggagacgc attgggtcaa cagtatagaa ccgtggatga tgtggtctct acaggatctg
     6181 acattattat tgttggaaga ggactatttg caaagggaag ggatgctaag gtagagggtg
     6241 aacgttacag aaaagcaggc tgggaagcat atttgagaag atgcggccag caaaactaaa
     6301 aaactgtatt ataagtaaat gcatgtatac taaactcaca aattagagct tcaatttaat
     6361 tatatcagtt attaccctat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     6421 cgcatcagga taggaaaaaa ccgagcttcc tttcatccgg cgcggctgtg ttctacatat
     6481 cactgaagct ccgggtattt taagttatac aagggaaaga tgccggctag actagcaagt
     6541 tttaggctgc ttaacattat ggataggcgg ataaagggcc caaacaggat tgtaaagctt
     6601 agacgcttct ggttggacaa tggtacgttt gtgtattaag taaggcttgg ctggggatag
     6661 caacattggg cagagtatag aagaccacaa aaaaaaggta tataagggca gagaagtctt
     6721 tgtaatgtgt gtaacttctc ttccatgtgt aatcagtatt tctacttact tcttaaatat
     6781 acagaagtaa gacagataac caacagcctt tcccagatat acatatatat ctttatttca
     6841 gcttaaacaa taattatatt tgtttaactc aaaaataaaa aaaaaaaacc aaactcacgc
     6901 aactaattat tccataataa aataacaaca tgtcgaccag ggaaaaacaa aacagcgcgg
     6961 acacagttct cgagaatgac gaggctgaat tggcgaggat gggttacaag caggagttga
     7021 agagggactt ggggttatta cagaattttg gaatgtcctt ctcaatcata tcggtcatga
     7081 ctggaatacc atcccttttc gcatatggac tcaataccgg aggaccggca gtgatggtat
     7141 ggggatggat catagtttcc gtgtttacaa tgcttgtggg cctggccatg gcagaggttt
     7201 gcagtgccca cccgacttcc ggaggaccct acttttgggc tgctatgtta tcgagtcctg
     7261 aagacgcacc actgctatca tggataacgg ggtggttcaa cttgttggga caagtcgctg
     7321 tcaccactgg aatcagcttt ggttgcgcta gcttcatatc aacaatctgc acattcaaca
     7381 cagatttcga gcctacacca aacactcaaa ttggtatata cgccgccgta ctcgtcagcc
     7441 aaggcctcat aaataccttt ggagtccacc tcctacatca cctcaacaac atctcagtct
     7501 ggtggcatgc tgctgggaca ttctcagtag tagtcgccat actagccaaa gcacccaccc
     7561 atcaatcggc gcatttcgtc tttcagacat tcctggatgg taccggtgga tgggcagaaa
     7621 gagctagccc agcttatgta gccgtcatcg ggattcttat ggcacagtat acgcttaccg
     7681 gattcgatgc tagcgctcat atgaccgaag agacacgcaa tgccgccatg tcgggaagtc
     7741 ttggcattat tatggccatt ggcgtttctg cgctgctagg atggtatctc atcctgggat
     7801 tgctgttttc gattcaaaat ttggaccgaa cactcgattc tccaagtgga caaccggtcg
     7861 ctcagatatt catcgatacc gttggcgtga atggcgcaat tgcacttatg gtagtggtta
     7921 ttggatgcat gtacttttgc gggacattct caataacgtc aaactctcgc atgatgtacg
     7981 cctttgcgag agatggtggc attcctggct cccaattctt tgcctatgtt aacacgaaat
     8041 ggaagtcgcc gatacgaacg gtctggcttg cctgcactct gagtttcatc ctcgggttgc
     8101 ccagcttagg tagcactgtt gcattctcag cggcaacatc gatcgccact attggattgt
     8161 acacctcata cggcatacct atcgcattac gaatcgtcta ccaccagaaa tttgtcaaag
     8221 gacccttcca tctacacaag ttctccttcc ccgtcgctat cacagctgtt ctgtggatcg
     8281 cgtttatctc gatcgtcttt attctaccac aagtcaaccc tgtcgattcg cagacgctca
     8341 actattcgat tgtcgctgtt ggcatcgtca tcatatatag cgtagggttt tggttgttga
     8401 gtgctagaaa gtggtttact ggacctgtca agcagattgc tgctgaggag atgggtatta
     8461 atgttatgga gccaggaaca gcagagaaag ttgaagagat ggagagggag aaggctcgta
     8521 ggagattgat aagacttttc tagttgcata tcttttatat ttaaatctta tctattagtt
     8581 aattttttgt aatttatcct tatatatagt ctggttattc taaaatatca tttcagtatc
     8641 taaaaattcc cctctttttt cagttatatc ttaacaggcg acagtccaaa tgttgattta
     8701 tcccagtccg attcatcagg gttgtgaagc attttgtcaa tggtcgaaat cacatcagta
     8761 atagtgcctc ttacttgcct catagaattt ctttctctta acgtcaccgt ttggtctttt
     8821 atagtttcga aatctatggt gataccaaat ggtgttccca attcatcgtt acgggcgtat
     8881 tttttaccaa ttgaagtatt ggaatcgtca attttaaagt atatctctct tttacgtaaa
     8941 gcctgcgaga tcctcttaag tatagcgggg aagccatcgt tattcgatat tgtcgtaaca
     9001 aatactttga tcggcgctat gcggccgcca ccgcggtgga gctccagctt ttgttccctt
     9061 tagtgagggt taattgcgcg cttggcgaga ctcaaacaag atatattttg cccgccttac
     9121 agacgaaact agttggaggt tatggagcat actatcacgt gggcggccac tggtgagtta
     9181 ctacacccca ggggcaacgt tgatgctcct aaaaaactct ggctggacgc aagccgtaac
     9241 acccgtgtca cttcataatc gtttgcaatt cagggcttga tctacacatt accctgttat
     9301 ccctattaat taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     9601 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     9661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9841 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    10021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    10081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    10141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    10201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    10261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    10321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    10381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    10441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    10501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    10561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    10621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    10681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    10741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    10801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    10861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    10921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    11041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    11101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    11161 cctgaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat
    11221 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct
    11281 attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg
    11341 ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga
    11401 gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga
    11461 gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat
    11521 aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact
    11581 ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt
    11641 actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac
    11701 cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg
    11761 tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt
    11821 tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc
    11881 taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc
    11941 aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa
    12001 gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac
    12061 agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa
    12121 gcgctctgaa gttcctatac tttctagaga ataggaactt cggaatagga acttcaaagc
    12181 gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac agctcactgt
    12241 tcacgtcgca cctatatctg cgtgttgcct gtatatatat atacatgaga agaacggcat
    12301 agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg atgaaaggta
    12361 gtctagtacc tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc ttccttcagc
    12421 actacccttt agctgttcta tatgctgcca ctcctcaatt ggattagtct catccttcaa
    12481 tgctatcatt tcctttgata ttggatcata ctaagaaacc attattatca tgacattaac
    12541 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
    12601 aaacctcggc gcgccattac cctgttatcc ctaatataca acgcaaagaa gctggaaaat
    12661 tggcgaggga atcctgtttc tgtctatcca agaatgggca tgaggtggca accgtcgtgc
    12721 tagcgtacag ggtgcacttt gtaaccattt gggacaccgg acactcgctg ttttcgaaat
    12781 taccctttaa gcgcgggtat tgaaccaggc ttatgcccaa gatcgtagca agcatttaaa
    12841 tgacggtatc gataagcttg atatcgaatt cctgcagccc gggggatcca ctagttctag
    12901 agttaattaa
//
