LOCUS       sequence-208215-       14360 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14360)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 14360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU74.1-2.2
FEATURES             Location/Qualifiers
     source          1..14360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             504..527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(8795..8815)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8795..8813)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(9152..9740)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9232..9251)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9911..10702,10703..10771))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10534..10553
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10772..10876)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10903..12245
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(11866..11913)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     12286..12304
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12576..12980
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             12993..14087
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(14335..14351)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14335..14351)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggattata aggatgatga tgataagact agtatggcat
      541 caggtgttgc tattgttggc atctccgtag agctgcccag tgggaactat tccgaggaga
      601 atttagacca taattctttc ttcgaattcc tcctgaatta cggcgaatcc tacgaacggc
      661 ttcctcgaga tcggttcaat gtcgaagctt ggagaggtaa cggtatcggg caaatccacg
      721 tagacggggg cacgttcctc aaggaaatcg actcgtttga caacatcgag ttcggagttt
      781 cgtcgcgaga tgcacgcgca atggcgccgg tcaccaagaa actgttggag aattgcttcc
      841 tcgcgcttct cgactcagga atcgactatc gtgcacggaa cgtgggctgc tacaccgccg
      901 gaaattcgtt cgagctgtca aatatcactg aaccagatga gtacgaaccg caaggatcgt
      961 tcgcgggata cgcggcaatg gtggcaaacc gcgtctcgaa ccatctcgac ttgcttggac
     1021 cgtccctacc tacggacact gcatgtagct ccacgctcgt agcgctgcat ctagctgtac
     1081 aggctgtgct gaatggcgat tgtgaggctg ccgttgtagg tggctgccag ctcaaccatc
     1141 gcctcatgga ttggatcacc tatagtcaag gctccctgct cgcgaaggat ggcaagtgca
     1201 agcccttcga cgcgtccgcg gatgggttcg caagagccga aggttgtgtc gctgtcgtgc
     1261 tcaaaacact cgaagatgct ctgcgggaca atgattatat ttatgctaca attcttggga
     1321 cagcgatcaa taatggcggc gctggagcac cacctggggc accagttgcg gacgcccaac
     1381 gtgatgctat gttgcaagcg tttcgtcgcg ccggaagatg tcctaaggac gtcgattata
     1441 tcgagctaca tgcaaccggg accgcgaaag gtgatcccac agaggccaat tgggttggca
     1501 aagagttcca gagggaagac gaggtgttga tcggcagtgt caaggggaat atagggcaca
     1561 cggagataac agccttcctg gcgtccttat ccaaggtgct ctcaattttg caacgtggca
     1621 ccatccctcc aaacgtgaac ctcaccgacc ctaatcccga gatcaagtgg gcagagtaca
     1681 ggctgaaggt accgacgagc ccaatgctgc ttccccgcca ttcgcccaac acgcacctcg
     1741 tctcgatggc tggctcgggc atcggaggct cgaacggcca cgtcgtccta gagggcccgc
     1801 ctctgcgagc acccgtgggt gcgcagcaac acctaaaggc gcgccgtccg gtgctgctcc
     1861 ttgctggcgg gctcaccccg cgaagtgcag ctgcagtagc ggagtcgatc attgcgtgca
     1921 tcgaaggcgg cgaggtcgac gtgtctgcga tgtcgacgat cctcgggcgc cgtgccagac
     1981 agatgacgtg gaggtcgtac gtagtctccg gcggggacac atccaattcg atcagctttg
     2041 gcgagcccca gcattgccca cgcaatgcaa atccgctcgt gttcgtcttc tcgggtcaag
     2101 gtccccagca catcaacatg gggcgcgaac tcttcaagac atttcctgtg ttccggaaca
     2161 gcgtgctgaa catggacaac acctacaaga ccgccactgg aaagtctatc atcgacgact
     2221 ggggcttgtt cggcgggaac tgctcaacag cactgccaga tgtgtggccg atctccctca
     2281 ccctgccagc catagcaatc ttccagatcg cattattcga cctccttgtc catctcggca
     2341 tcaaccccga cattgtcatt gggcactcag ccggcgagac ggccgtgctc tacgcgtcgg
     2401 gcgctgcgcc gaaggcaatg acggtcgaat tggccgttct gcggggcgtt atcttcaccc
     2461 aattggaaca ccagggaggt accatggccg cgctatcctg cactgcgcac gaagcgatgc
     2521 agatactgga ccaggcctgt gcgcaggagc cggggtgcgc ggttgagatt gcctgctata
     2581 atgcgccggc ggcggtcgcc atcgccgggc acatgcgcgc ggttgatcgc gttgtagagc
     2641 ttgcgcaggc ccgtgggata tacatacgca agcttcctac tcgggtgccg atccattcgt
     2701 cactgatgga tgcgtgcagg gaagagtatt gcggtggctt gtatgagctg ttcgcacgtt
     2761 accctggggc gcatgtccca aagattctga cctactcgac gcttacggga gggccgtttg
     2821 ggggcccatt cgatgccgac tacttttggc agaacacgcg cggtgaagtg cgcttcaccg
     2881 aaacagtgga agcgattgct tcgtacggaa gtcatacttt cgtagagatc tctccacgac
     2941 ctgctttggc atcctacgtc cggtccatgg cgcaagagcc gaacattgtc ctcaccaccg
     3001 tgcgccggca gaggtcggga caggacccat cggaatatcg cgacgtcctc gaactctgcg
     3061 ggcaactcac tattactggg cataattgcg ttgactttac ggtactcaac ggtcgagcgt
     3121 gctatgaagt acaggtccct ttaccttctt atccattctc gaagaagcga ttcccgttgt
     3181 atcctgacac tgccggctac agaaaacagc ttgagcctcg tcgaggacct ttgaaccata
     3241 gacatctccg cgtcaacaag gagtcacatc cctcactggg tgaacatgtc attagaggcg
     3301 agccgataat gcccgctgcc gggttcattg agatggccct agaatttgga gcacgcacac
     3361 tcatcaatgt caacctgcaa tctatcctgt cgctttctgc ggaaaggcca gtatcgttac
     3421 aagttcgact ccaaggctca ttttggactg tatcatccac ggttccaacg tccaagatct
     3481 cctcaaatat agactcagat catatcgaac gactacatgc cgatggattc ctttcctttg
     3541 aagcagtacc aacatatccc gatctcgatg tatctggact ccggaaacat cttcagaaca
     3601 atgtcggctc agatttctat ccctcacttt cctactattc ttcatatggt ccgaggatgc
     3661 gacgcgtgac gaacgtatac tttggccgaa acgaggcact ggcatcaatc tgcggaatgg
     3721 atgacatact ctccgaggat gccgcctatt tcttacaccc tgcaattctg gatgcttgca
     3781 tacaagtctc cgcatacaag cctttccacg gcaatttcga ccccaacgtt tactatctac
     3841 cagctcatgt tgacgcagtc ttcgtccatg agccacttaa gaccagctat tttcctgccc
     3901 atctatacgc tcatgttgaa ttgagggcgt ggcatcccga ccatatgcga tacgacgtct
     3961 ccctcgtgaa cgattttggg aagaggctat gtacggtcaa aggcttgaga atgacaaaac
     4021 accacatcaa tccgttggca agtgtctccg gccctctgga gcttgttctt cagcctattt
     4081 ccatcctatc acttccgacg acatctggaa ggatctccac tgcacatcaa aacacccagc
     4141 tcttcgagat atctgacgaa atagagctgc accatcaata ccgaaccaat ggggtacacg
     4201 actgctccga tgtccagtat tggtgccata gccgtgacat tcaagctctt aatctagaga
     4261 gtcaaagtgg actctcctca cacgaggaca cgaccggctc cgtaattgaa ggggtgttgc
     4321 acaccatttt gagccacata tccgatcaat cacatatacg tgtgatccgg gtcttgatct
     4381 ctcgcttgca tcccataatg ctcgaatctt tcgctcaggt cttgaagggg ttcccggctg
     4441 tcttcttcga gatatacatc agctcggacg acacaagtgc gacgcttcta gtagaagact
     4501 tctcagggat tgttcgcact gctgcgactg acatccagcg ctcatttttc gaaatgatct
     4561 tcacatcgcc aaatgacttc ccagaggcag tgatacagtc atttggtagc atgttagtac
     4621 ctggaggcgt gattgtattt gccgaaggcc ttcctaagcc tctgggccac agatacctca
     4681 gcctcgacac cgtccgacac gtgtcatcaa cacccacact cccactcgaa acgctggaga
     4741 agttgggttt ttctgtggta cagccccatg gccccgactg tgctattgcg cagaattgta
     4801 ccgccgatgc tctccaagcc accgaaccct tatatgatcc actggaagca ttcgtattcc
     4861 aatacgaact cagaaacgag gtcgaattgc agtgggaatt tagcgggctc aacattaggc
     4921 aagagctgga tgtctggatc ctcgcttcgg aaggaaaaga tggtggatct gcggtgggaa
     4981 tggtacgggc cctgagaagg gagtattcat cgtggacaat ccgccttgtc gtcttcccac
     5041 tctcgtaccc ggaagagatg cggctccaat gtcttcgccg acttcccacc tacatgaaag
     5101 aggaactaga cattctcgta tcccgtacag gagacttcac agtctttcga ttggcccctg
     5161 tcggcaggcc gccccatgat cacaatcctg cccgctggaa cgacacgacc agtgatatcc
     5221 cccacggaca tgttctcgta catgttctgt attcgtccag ccactacgat atttcagcat
     5281 tggtggcgtc cgtagacaat gccaacaata ccggtatccg agagggatcc atactattgg
     5341 ccatgattac aggtaccccc ggaacgtcgc tcgtcttgga tgttactatc acttgctcag
     5401 taccgccatc tctccttgag caagtatcga tggttgtgca aatgatggcc ccgctcgtga
     5461 cggctgtcct tgctcccggt ctagccgcct tcaggtcgcc agctcgtctt cgaacattac
     5521 gaatcttatt gactcatgct gatatcccat ttgggcgaat cataaagagt atctatgcgg
     5581 aaaaggcgac actcactgaa gtagtcgaga atatcactct ctttgatcta gcgtcactcg
     5641 agagtcagcc atttgacttg atcatatccg gatacgacga tcacgcccac agtcaagtcc
     5701 tccgttccat actcagaccc ggccgcggaa gactgttttt gtggggcaat cgtcaaggtc
     5761 tccgcgaact catagataga gagctataca cagttgtgga tgccatgaac tgcgttcttc
     5821 agtctcttga cgagcaaccc cgcatcatat gcgaaaacac atcaattacc ccttcattgg
     5881 cgccatccga tgtcatcgtg acacaagtca gcccacaggc catatccact ggagtcacct
     5941 tcagtgccga caaaacatat ctgcttgtag gaggcattgg gagcattgga gcgcgactgg
     6001 cactctactt gtacgagaga ggggctcgac atattgttgt ctcatcgcgt accggggcaa
     6061 ggggccttcg cacaagtaac aatcttatgg tgtatcgaat attcgaatat ctcaagggga
     6121 tcagcgacct ggacatccgc ctcgttgccg tagacgcatc ttcacgacac gaaatgaaga
     6181 cgttgcgtga caacatcaat ccgaaaatcg ccggatgtat catcctcact gctgtactct
     6241 ccgaccgtgt gttccaatac ctcgaggaag agcagtttac tgccgttttc gctgctaaga
     6301 ttggagttct cgatgctctt attgacacga tagacgtatc gagtctcgag tttctcgttg
     6361 ccttttcttc cgtctcaggc ttgttcggtg tcggtggtca gtcgaattac ggcgcagcga
     6421 attcaactct agaggagacg atgtcagtaa tctctaacgg gttctgcttc gtgtgccctg
     6481 ggattctcga ctcgtccttg atgctggcta atgggatgga taccgagaag catcgccttg
     6541 ctcacttcat agagtggtct atcacaacag atgaaatggt tctgtggttc gatgacgcga
     6601 tgacgagata ccagggtggc caaaagttca gtcgttacat ccccaatctg aattgggagg
     6661 tgatggaccg cacgcacgga atgaacaaac tgggagcaca tctagttccg acgcgcacgg
     6721 ctaaggcacc tactacggag gacgacacag gtcgagtggc agagattgtc agaagcacct
     6781 tgaatatatc ctcagacgac ttctccgcag aagtaccttt caccgcatac ggtctagact
     6841 cgctctccgc ttcaagattg tccttcatgc tgcgtcctat gatgagcgtt acccagatac
     6901 aactcctagc ggatatgacc ctcagagatc tccagactag actgtccgcc gaaccagtgt
     6961 cggaggattt tcgggcccta gagccgacca cgggcaagaa tgaaatacaa gcgatgcgcg
     7021 acatgctcag caaatatacg caggatttac gggaacaaag agctacaacc gacatccgag
     7081 cgacgcgcaa cgatgttgtc ctcctcacag gaagcactgg cgctctcggt gccaacattc
     7141 ttgctcagct cctcgacaac agcgatgtca cgaaggtttt cgccctgaat aggaaaggca
     7201 ctgatcgcca ttgcctgatt gagcgacaag tggctgccct cgtgacacag ggcctgccgc
     7261 ctacgttggc cgaatcgcag aaactggtgc tgatcgaagg agatttactg ctggatggac
     7321 ttggtattcc actagaggtg tttagtgaga tgctatcctc agtgacacac gtaatccaca
     7381 atgcctggac catcgacttc ttgtcctctc tggaggcaat ggaagatctt gtaaggggca
     7441 cccgtcggct attggacatt gctgctctct cgactcgtgc gactcctcca tcattttcct
     7501 ttatcagttc tattggcatt taccagcatt attcgggttc ggctccagca ccagaggagc
     7561 ccgtgctcga tgcgaagatg gccgtacaga ccggttacat ggagtccaaa tgggtggccg
     7621 aacgaattgt tcagatagct ggcgctagac tcgggctcaa agcgcacgtg attcgagtcg
     7681 gactcctgac aggaggcgtc aatggctcat gggatcctac acactggctc ccagcgctcg
     7741 tgcagtctgc aacgtacgta gggtgcctcc cagaaagcga tagcctcgtc tcatggatcc
     7801 cggtggacgt cgctgctgct gcggtagttg atctacgcca cgcctctgat gatacactcc
     7861 atgtgattca ccctcgcccg atgctttggt cagacatcat ggttcccata gcatcgctgc
     7921 tcgatgttcc acttgttccc tacatcgaat ggttctgtcg actcgagaac atggccaacg
     7981 caatctccca tgcacctagt cagcgagaca gccggcggct gaggcacgcg ctcagactta
     8041 ttgattttta tcggctggga atgcgaggca gtaagggagt ttctgggtcc acggagagca
     8101 tgggtctatt gccgcaagtg aaatgtcaga aggcaatgcg aagttctgcg acactgcaga
     8161 atcccgaact atgtcaattg gggatcagcg acatcgaacg atgggtgtct tattggcgac
     8221 aagccggtca tctgccaaat aatgactcgc caaactgagg agattgataa gacttttcta
     8281 gttgcatatc ttttatattt aaatcttatc tattagttaa ttttttgtaa tttatcctta
     8341 tatatagtct ggttattcta aaatatcatt tcagtatcta aaaattcccc tcttttttca
     8401 gttatatctt aacaggcgac agtccaaatg ttgatttatc ccagtccgat tcatcagggt
     8461 tgtgaagcat tttgtcaatg gtcgaaatca catcagtaat agtgcctctt acttgcctca
     8521 tagaatttct ttctcttaac gtcaccgttt ggtcttttat agtttcgaaa tctatggtga
     8581 taccaaatgg tgttcccaat tcatcgttac gggcgtattt tttaccaatt gaagtattgg
     8641 aatcgtcaat tttaaagtat atctctcttt tacgtaaagc ctgcgagatc ctcttaagta
     8701 tagcggggaa gccatcgtta ttcgatattg tcgtaacaaa tactttgatc ggcgctatgc
     8761 ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct
     8821 tggcgagact caaacaagat atattttgcc cgccttacag acgaaactag ttggaggtta
     8881 tggagcatac tatcacgtgg gcggccactg gtgagttact acaccccagg ggcaacgttg
     8941 atgctcctaa aaaactctgg ctggacgcaa gccgtaacac ccgtgtcact tcataatcgt
     9001 ttgcaattca gggcttgatc tacacattac cctgttatcc ctattaatta atacggttat
     9061 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     9121 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     9181 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     9241 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     9301 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     9361 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     9421 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     9481 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     9541 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     9601 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9661 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9721 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9781 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9841 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9901 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9961 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    10021 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    10081 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    10141 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    10201 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    10261 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    10321 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    10381 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    10441 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    10501 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    10561 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    10621 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    10681 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    10741 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10801 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10861 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaacgaagc atctgtgctt
    10921 cattttgtag aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc
    10981 tgcattttta cagaacagaa atgcaacgcg aaagcgctat tttaccaacg aagaatctgt
    11041 gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc
    11101 tgagctgcat ttttacagaa cagaaatgca acgcgagagc gctattttac caacaaagaa
    11161 tctatacttc ttttttgttc tacaaaaatg catcccgaga gcgctatttt tctaacaaag
    11221 catcttagat tacttttttt ctcctttgtg cgctctataa tgcagtctct tgataacttt
    11281 ttgcactgta ggtccgttaa ggttagaaga aggctacttt ggtgtctatt ttctcttcca
    11341 taaaaaaagc ctgactccac ttcccgcgtt tactgattac tagcgaagct gcgggtgcat
    11401 tttttcaaga taaaggcatc cccgattata ttctataccg atgtggattg cgcatacttt
    11461 gtgaacagaa agtgatagcg ttgatgattc ttcattggtc agaaaattat gaacggtttc
    11521 ttctattttg tctctatata ctacgtatag gaaatgttta cattttcgta ttgttttcga
    11581 ttcactctat gaatagttct tactacaatt tttttgtcta aagagtaata ctagagataa
    11641 acataaaaaa tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag gtggatgggt
    11701 aggttatata gggatatagc acagagatat atagcaaaga gatacttttg agcaatgttt
    11761 gtggaagcgg tattcgcaat attttagtag ctcgttacag tccggtgcgt ttttggtttt
    11821 ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc gctctgaagt tcctatactt
    11881 tctagagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc
    11941 gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg
    12001 tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg
    12061 cgtacttata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt
    12121 atcccattcc atgcggggta tcgtatgctt ccttcagcac taccctttag ctgttctata
    12181 tgctgccact cctcaattgg attagtctca tccttcaatg ctatcatttc ctttgatatt
    12241 ggatcatact aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
    12301 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctcggcgc gccattaccc
    12361 tgttatccct aatatacaac gcaaagaagc tggaaaattg gcgagggaat cctgtttctg
    12421 tctatccaag aatgggcatg aggtggcaac cgtcgtgcta gcgtacaggg tgcactttgt
    12481 aaccatttgg gacaccggac actcgctgtt ttcgaaatta ccctttaagc gcgggtattg
    12541 aaccaggctt atgcccaaga tcgtagcaag catttaactg tgggaatact caggtatcgt
    12601 aagatgcaag agttcgaatc tcttagcaac cattattttt ttcctcaaca taacgagaac
    12661 acacaggggc gctatcgcac agaatcaaat tcgatgactg gaaatttttt gttaatttca
    12721 gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga
    12781 gaggccggaa ccggcttttc atatagaata gagaagcgtt catgactaaa tgcttgcatc
    12841 acaatacttg aagttgacaa tattatttaa ggacctattg ttttttccaa taggtggtta
    12901 gcaatcgtct tactttctaa cttttcttac cttttacatt tcagcaatat atatatatat
    12961 ttcaaggata taccattcta atgtctgccc ctatgtctgc ccctaagaag atcgtcgttt
    13021 tgccaggtga ccacgttggt caagaaatca cagccgaagc cattaaggtt cttaaagcta
    13081 tttctgatgt tcgttccaat gtcaagttcg atttcgaaaa tcatttaatt ggtggtgctg
    13141 ctatcgatgc tacaggtgtc ccacttccag atgaggcgct ggaagcctcc aagaaggttg
    13201 atgccgtttt gttaggtgct gtggctggtc ctaaatgggg taccggtagt gttagacctg
    13261 aacaaggttt actaaaaatc cgtaaagaac ttcaattgta cgccaactta agaccatgta
    13321 actttgcatc cgactctctt ttagacttat ctccaatcaa gccacaattt gctaaaggta
    13381 ctgacttcgt tgttgtcaga gaattagtgg gaggtattta ctttggtaag agaaaggaag
    13441 acgatggtga tggtgtcgct tgggatagtg aacaatacac cgttccagaa gtgcaaagaa
    13501 tcacaagaat ggccgctttc atggccctac aacatgagcc accattgcct atttggtcct
    13561 tggataaagc taatcttttg gcctcttcaa gattatggag aaaaactgtg gaggaaacca
    13621 tcaagaacga attccctaca ttgaaggttc aacatcaatt gattgattct gccgccatga
    13681 tcctagttaa gaacccaacc cacctaaatg gtattataat caccagcaac atgtttggtg
    13741 atatcatctc cgatgaagcc tccgttatcc caggttcctt gggtttgttg ccatctgcgt
    13801 ccttggcctc tttgccagac aagaacaccg catttggttt gtacgaacca tgccacggtt
    13861 ctgctccaga tttgccaaag aataaggttg accctatcgc cactatcttg tctgctgcaa
    13921 tgatgttgaa attgtcattg aacttgcctg aagaaggtaa ggccattgaa gatgcagtta
    13981 aaaaggtttt ggatgcaggt atcagaactg gtgatttagg tggttccaac agtaccaccg
    14041 aagtcggtga tgctgtcgcc gaagaagtta agaaaatcct tgcttaaaaa gattctcttt
    14101 ttttatgata tttgtacata aactttataa atgaaattca taatagaaac gacacgaaat
    14161 tacaaaatgg aatatgttca tagggtagac gaaactatat acgcaatcta catacattta
    14221 tcaagaagga gaaaaaggag gatagtaaag gaatacaggt aagcaaattg atactaatgg
    14281 ctcaacgaaa tgacggtatc gataagcttg atatcgaatt cctgcagccc gggggatcca
    14341 ctagttctag agttaattaa
//
