LOCUS       sequence-208216-       14894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14894)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 14894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU75.1-2.2
FEATURES             Location/Qualifiers
     source          1..14894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             504..527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(1984..1995)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9329..9349)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9329..9347)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(9686..10274)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9766..9785)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10445..11236,11237..11305))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11068..11087
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11306..11410)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11437..12779
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(12400..12447)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     12820..12838
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13110..13514
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             13527..14621
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(14869..14885)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14869..14885)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggattata aggatgatga tgataagact agtatggttt
      541 caactccatc ctctgccatt cgccccagct atgccaccac tgactctcat gccatggcca
      601 tcgtcggtac ctccatcgct gccccaggcg gcgtcgacga tggactagac actgaggagt
      661 tctatgagtt cctcaaatct cgtggctccg gtatcatcac tgtacccaaa gatcgctgga
      721 atgctgaagc ttatcatggc gctcaacctg gcaaaattgt tactaccaaa ggtggttaca
      781 tcccaaacgc tacatacgga gacacgcaag aattcggtat cactcctgca gaagctgccc
      841 agatggctgt ttctcaaata gctttgcttc accacgcctt caacgctttg caacgcagtg
      901 gagtcgacta ccgaggcacg aacactggcg tgtatgtcgg ttgcacgact ggtgcagcac
      961 cccacaacgt agacatcacc gaagccggag catactacat gaccggtagc tcgatatcta
     1021 tcaatgcgaa tcgtatcagc tacgttttcg acctgactgg tccttccttg ccagtcgata
     1081 cggcctgctc ttctagtttg accgctatgc atctcgctgt gcaagctgtt aggaatggag
     1141 aatgtgacca agctgttgtt gctggagtca acatccttct cagtccactt gagactgcat
     1201 ccttcagtca gctcggagtc ctcggcccag atggtatctc gaaagctttc gatgaagatg
     1261 caaacggtta cgcgcgtggt gatgttgtca gtgccgtggt tatcaagcgt cacgatcttg
     1321 ctgtcaggga ctatgaccgt attctggcca ctctcgtcgg cactgctctt acgtcctgcg
     1381 gatccttgat gggatctctc actacaccta gccccgatgc tcaaaccaag gctattagga
     1441 atgcctacgc cgatgctggt ctaacaccca accaagccga cttcgttgag ttacacggta
     1501 ctggaactat cgtcggagat cagtacgagg ccaatacggc aggcgcatgc ttctctgacg
     1561 gacgtgacgg tcgagaaatc gttatcggct cagtcaagag caacgtcggt cacggtgaaa
     1621 tgggtgctta tatgagctct ttggtcaaag ttgtcatgat gttggaacgc aagcagattc
     1681 ttccgaacgg atacttcaag aagccttctt cgaggatcca atttgacgag tataagctgc
     1741 gggtacctat cgaagtcgag gactttgttc cctatgacgt cgagcagggt ctaatcgcgt
     1801 cgatttcttc cttcggattc ggaggatctt gtggtcacac tgtcctgcga gaacacgatg
     1861 ttaggcccgt cctccctgac tacgaaacgc taaactcggg accatatctc ttcgccgttg
     1921 gaggtcttac tcctcgttca gtcaacactc tcatcgagag ctacaaagcc gagtacgcgg
     1981 atgtcgaccc tctatcccta tctgaacacc tcggctcgcg tgctcgccaa ttgagctggc
     2041 gttcattcgc agttgcagat tccttgggcc aggcaaagtt cactgaacct gtcatggtat
     2101 caaaacgtgc tgttcctttg atcttttgct tctctggtca aggccctcag cactggcaac
     2161 agggtcgcga actcttctcc cgatactctg tcttccgtga aagtatcttg gcttccgaca
     2221 aaatttacac cgagtacaca ggaaagtcct tccttgaaac ctccggcctc tttgtcccca
     2281 accctcctgc gaactctatc ctcgccaagt ccctttcatg gcccgcagac gccatctctg
     2341 tttccatcac tttcttccag atcgctcttt tcgacctttc gatctacctt ggactcaagc
     2401 ccgacgccat cgttggacac agtatcggcg aaactgctgt tctttatgcc agtggtgcta
     2461 tgcctcgcga gatggtcgtc aagatcgccg ttgctcgagg tgctgctctt cgcctcgttg
     2521 acaacattgg tggagccatg gtcgctgtct ctggttgcga cctccgaaca gtccaagatt
     2581 atgccgaagc agtcgttgac ctttccaaca acccgacggc gggtgttacc gactctctcc
     2641 acgtggcagc tcggaatagc ccgaccgact ttggtctgtc gggtgctgag tatctcgtcg
     2701 acgaactggc caagtatatc gaccaatggg tttctggagt ctctgctcgc aagcttcgtg
     2761 tcagcactgc tgttcactct ccttatgtcg atgcttgcga ggcccaatac cgtcaagagc
     2821 tcgctgcaat ctttgaagca caccctggta cccacaagcc ttcgcttcca gtgatgtcaa
     2881 cagtgacagc cgagttcgtt gagaaggagt acacaatcga ctacctctgg aacaaccttc
     2941 gtcaacctgt tcgcttctct gatgctattc ccaagctcat cgagaagcac ggtgaaaaca
     3001 cgaccttcgt tgagattgct cctcatcccg tcttgtctca atacatcaag caaatgggtg
     3061 ccttcgagtc ggtcgcgggt agcaaacgtc ctccttcggc tcgtcacatc aaggctggtc
     3121 aacgccctcc taccgagctc gatgcactgt acgaagggat tggccagctg ttggcttcag
     3181 gtgtcaactc ggtcaacttt gcactcctta acggttgtcc tccagagaac ttccttggac
     3241 ctaagtaccc attccaaatg aagtacttcc cggtcgcacc tcgtgttccc agctatgtgc
     3301 gcaagctgct ccctgccgag agaccgctca acagcgaacg gctccgtatc agcccccatc
     3361 tgcctgaaga ttggatggct gatcatgtta tcgatcactc gaacctcatt cctgctgctg
     3421 cgtatcttga aatggctctc gagttccctg acgtcacttc agtctgggac tgccgtttcg
     3481 agtctgcttt cattctcgat ggttcggtgc ctgccgcaac cttgaaagta tctcgggacg
     3541 gaaacaggtg gagtgtcaaa tcttcaagcg ctctccaaag catgcaaggc gacctaacat
     3601 ggacccgttc tgagccgtcc ttcgactcta tccatgccta cggcaagctt ggatacggca
     3661 agccggagtt aagacctgga gctatcaccc atgttgatgt tgatgctgtg attgctcgct
     3721 cgaaagtaca agcttcgcat gaagcacttt acgatgaatt gagtcaagtt gcccaatttg
     3781 gaacagagtt ccgacggctc cagaaggcct acgccaatga tgtggacggt attgtttaca
     3841 ttaaaggtca cggtgacagc ttgactcgta ctgggtacac attccatcct gctctcatgg
     3901 atgcagtatt ccagtccggt gtctgtttta acatggtgtt cgatcatgtc aacgtcggaa
     3961 accctgagcg tacattcatg ctgccacact ccttgcgtcg cggatttagg aatgatggaa
     4021 gcacagaacc gcttatcttc aacggtgact tccgtatcta cgctatcctg gctgattggt
     4081 cgccgtactc ggcgacatat gactactatg tgctgaacga cgataatgat gtcgtcttca
     4141 cttttgaagg cctgcgtttc gaaacggtgc agcaagacca acaagcaatc aaagaacgtt
     4201 tcagcatgat ttggcaaccc cgtggcttgc ccaagtcgaa taacagtggt catgttacac
     4261 ttgaggatga ttcggttttg gaacaccaag agttgcttga tgttctagac aagctcgcct
     4321 tgggttacac caagaaggct ctagggcgcc tttctcagaa cttcgaaacg gctctgcctg
     4381 accgtaaacg ttacatggaa tgggcactcg agcgaactgc agaacgggct actgaaacct
     4441 tcttggagtt gagcgatgta tctccggtga tgaggcagaa gtatgcttct ttgttcgaac
     4501 tcacgcagcg agtcggagaa gggcagacag acattttcgt agattcaaag gctgccgtag
     4561 acatcctctt ccgagatgac ctcatgagcc gaatttacga gcatccacct ttcatcggca
     4621 gtatattcga cgaaactgtc aagcaattcg tcaagctcgt caaggatgcc atagaagccg
     4681 gcaaacgcgt tgttcgtgtg ctcgaggtcg gtgctggaac cggtaggttc acggctttgc
     4741 tcggtcaagc tcttctagat gcccagttgg aactgtgcta cgtcgactac gtttgcacgg
     4801 acatttccat ttctcttgct caggaggcca cagccaagtg tccctggatg acaattgtcc
     4861 ctgttgcatt cgacctcaac aagccacttg atcaacaaag tatcgatccc gcgagctttg
     4921 atattattgt cgcattcgat gtgctccacg cgacaccaaa cattaacgat accgttcgta
     4981 ctctccgtga gctcttactt cctggagggc atcttgcaat catcgaactg gatggaaact
     5041 cattcgccac tggtgctgtc ggaagcatct ggatggatta cgtctttggc tctttctcag
     5101 agtggtttgg tgttttggaa cagcgtccgg cctctcactg ctctctgagc aaatcagagt
     5161 ggaacagcgt cctgcattcc gaaggattcg ctgaggggct cttcctcaca tcaaccgggg
     5221 ctactgtttc tcatatggca ttcgtgtccc agaccactga gacggctcac gaacaacaaa
     5281 gctctccaac tccttctact ccctcgctgc aaagcgacca cagtcctcat cttaggtcat
     5341 tctctttctc ctcaacgccc ctccccgtta cacccaatac tcctggcttc agtggagatc
     5401 caacccgcag tgtcttgtca atcgacaacg agcatcaact gtcgaagaag ttggagggtg
     5461 gggccacttc cgctgacaag caatcgcaga tcattgctcc aggcccaatc acacttgaag
     5521 accaacgctc gtcgtctcaa tctccagagc tgtcactacc tcccaataca atcattcgtc
     5581 gattcacggc tggggatgag gtcgagttag tcagcttcgt cgctagtctc gactcctccc
     5641 ttccgttgaa gatttggctc tacactgaca cacagagcga caacgctacg ctgatcggac
     5701 tgaccagaac gctcaggcat gaattcggtt tctggaaggt gtatcttatc ctcttccatc
     5761 cttcttggag ctttgccaac caagaagagt tcattcgtaa ccagcttatc cctcttaagt
     5821 ggttggacgc ggaaatcatg gttgatgagg atggcaccat gcgagtgcca cgtattgtga
     5881 caggcgctgc cccgtcaacc acggagatgg ctgattccaa gccactgcaa ttcgatgact
     5941 cccagttttg gcgagcgctc cctgccccac tgaatgcgga tgatgtccaa gtcgctgtgt
     6001 cctttatcac actttccagt gtaattccag gtcattccga gttctctgga aaagtcacca
     6061 gtgtagggtc agaggtggaa acctctttga ttgggaagcg cgtgatgggt gtcaccaagt
     6121 cacctaaagg caactttgtg gtttgtccta aaggttgcat aacgacgatc ccggatagca
     6181 tgagccttcc cctcgctgct gctcttgccg gccgcctcgc attcacatct tcagttgtgg
     6241 ccgatacctt ggcgaatgcc aaaggtggcg ttcgtgtaat tctccacgcc gctgactgtt
     6301 ctccagctgc cgtcgcaaca tacatctatc tccaggagcg caatttcgat attttcgtga
     6361 cagcagcgaa tccgtctaca catcagattc ctcataagga agttttcgac tcaggcaatg
     6421 ttgagatttg gtcgcatgct gcacgacaat gggctgctga aggtgccgac ttcatcttca
     6481 acttcgaaga tggaactgta gtccttgagg aatcgattga gaggctagct cgtcgtggca
     6541 ccctttgcca agtcggaact ctctatcctc accgcctccg tccaggtcag cgattcgtat
     6601 ctgttgaatt tgactgtcta ttctctggcg aacataacgt tctggaagaa gccattcagt
     6661 caacttccag tcagactatt tctcgtattt ccccctcaac tctcatcttc caggcggatg
     6721 ctctccaatt ggcacactca aaagccagcc tatacaagga tctctcgatt atgttggact
     6781 tcgagacgtt accgaacaac atgactgtct ccaagcctgg tagactgcgt ggtacgaata
     6841 catttgaccc tcgtgcatcc tatgtcgtta tcggaggcat cggtggtctt ggaattgaga
     6901 ttgcaagatt gctgatcgag aagggcgccc ggcacgttat tctgacatca cgatcgggta
     6961 gcaaggcatt cgacgatggt cggcttgttc gggagaggag ggtcctcaac ttcttgcgcg
     7021 agaagcccgg ggttactatc gacctggttg ccgtggactg ccttgatgcg gcaaagacga
     7081 aaactctttt ctccaactta ccgaatgtcg ctggtgtctt ctacgtcgcc gtgagattaa
     7141 acgactcgct attcctcaat ctcactaccc aggaagactg gtcaaaagtg tacgatgtca
     7201 aggtcaaggg tcttcgcatt ctcttggatg ctgttgatcc cagaaaactc gatttccttg
     7261 tcctgacgag ctctatggca accgtcagcg gttctcccgg tcaagccaac tatgctgcag
     7321 cccagaccga aatggaggcg atgggtgcaa acattcccaa ttgcatttca gtcaccgttc
     7381 ctcctctaac cgacggtggt gtctttgtac gaagtatgcc tactggcaat gctcgtaacg
     7441 cggctctcga caaatataag gatctcggca tgactggaat gaagttggcc cgtcattgcg
     7501 tcgatgctat ctggactatt ggaacgccag cctaccaacc agtctacatt cccgcgacca
     7561 actggaagaa ggtcatggag gtcgctgctc ctgaatacct tcaatctagt atgagacact
     7621 tgttggtcaa agagaccact gacagtgtag cagccgacgg cggcaaagaa caaaccattc
     7681 ttggagcctg tgcggctgtg ctatcgcttg acattgaccg cgtcgaagat aatattcctc
     7741 tcgctagcta cggcttggat tcattgacgt cggttcgcct cagtggtgtt ctcaaggctc
     7801 gtttcggggt tgaagtcaca cagctgcagt tgttgagtca gactatgaca gttgcgcgct
     7861 tatgtcagct ccaggaggaa caacaagcat ctgtcaccac aaccgccacc gccaacgatg
     7921 accgcaccca agtcgatccg gccaaggtgc atgaagcgga tatggatcaa actattgtgc
     7981 ggctcaacac tccaaccgaa ggcactcctc tcttcctcat tcacggtgct ggtggaggta
     8041 tcgtcgtact tgtcaaagca gcccagaggg ttcactatcc tgtctatggt gtgcaggata
     8101 ctccagaggc acccatcacc ggtaatctcc gccgattgtc agaattctac ttgatgaaga
     8161 tccgggagaa acagccacac ggtccttaca gacttggagg attctcattc ggaacgtgct
     8221 tggctttgga catttccctt ttactgcaac aacagggcga ggttgtcgaa tcattggtgc
     8281 tcttggatgg ttcgcctact ctcttcaagc gacctgcatt ccagtcatat ctcatgggac
     8341 gcatccaaga cggcagtttc caggaagaca tcctcgatgt cgtcaacgac atggcgtcta
     8401 gtggaacgct cgatgacgcg gaggatttag gccgtcagtt tgctgaccac ttccagcgtg
     8461 ccaaggaagg tggaagtgga ccaaaatggg tcgctcgctt ctgcactgcc tatgtcgctc
     8521 acctcattat gggtcttcgt tgtggtaaag agttcaacgc tttagaggcg aaatacggaa
     8581 aggatgcgat gggtgcttgg ccagcaaaga ggaccgtgct cgtgcgtgct ctcagcggtg
     8641 tatctgctga accttatgca gccggtgctt cggagtactt tgacttggac gtctatggct
     8701 caggcgtgga gaagcatgac ctgcctggca cgcacttcgg catccttgca cccaaatccg
     8761 gactcgacgc cattctcgac aaactgcact aaggagattg ataagacttt tctagttgca
     8821 tatcttttat atttaaatct tatctattag ttaatttttt gtaatttatc cttatatata
     8881 gtctggttat tctaaaatat catttcagta tctaaaaatt cccctctttt ttcagttata
     8941 tcttaacagg cgacagtcca aatgttgatt tatcccagtc cgattcatca gggttgtgaa
     9001 gcattttgtc aatggtcgaa atcacatcag taatagtgcc tcttacttgc ctcatagaat
     9061 ttctttctct taacgtcacc gtttggtctt ttatagtttc gaaatctatg gtgataccaa
     9121 atggtgttcc caattcatcg ttacgggcgt attttttacc aattgaagta ttggaatcgt
     9181 caattttaaa gtatatctct cttttacgta aagcctgcga gatcctctta agtatagcgg
     9241 ggaagccatc gttattcgat attgtcgtaa caaatacttt gatcggcgct atgcggccgc
     9301 caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcga
     9361 gactcaaaca agatatattt tgcccgcctt acagacgaaa ctagttggag gttatggagc
     9421 atactatcac gtgggcggcc actggtgagt tactacaccc caggggcaac gttgatgctc
     9481 ctaaaaaact ctggctggac gcaagccgta acacccgtgt cacttcataa tcgtttgcaa
     9541 ttcagggctt gatctacaca ttaccctgtt atccctatta attaatacgg ttatccacag
     9601 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9661 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9721 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9781 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9841 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9901 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9961 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    10021 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    10081 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
    10141 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    10201 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10261 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10321 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    10381 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    10441 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    10501 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    10561 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    10621 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    10681 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    10741 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10801 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    10861 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    10921 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    10981 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    11041 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    11101 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    11161 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    11221 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    11281 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    11341 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    11401 gggttccgcg cacatttccc cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt
    11461 gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
    11521 tttacagaac agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca
    11581 tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct
    11641 gcatttttac agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata
    11701 cttctttttt gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt
    11761 agattacttt ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac
    11821 tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa
    11881 aagcctgact ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc
    11941 aagataaagg catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac
    12001 agaaagtgat agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat
    12061 tttgtctcta tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact
    12121 ctatgaatag ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa
    12181 aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta
    12241 tatagggata tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa
    12301 gcggtattcg caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa
    12361 gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctaga
    12421 gaataggaac ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat
    12481 gcaacgcgag ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc
    12541 ctgtatatat atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact
    12601 tatatgcgtc tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca
    12661 ttccatgcgg ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc
    12721 cactcctcaa ttggattagt ctcatccttc aatgctatca tttcctttga tattggatca
    12781 tactaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
    12841 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctcg gcgcgccatt accctgttat
    12901 ccctaatata caacgcaaag aagctggaaa attggcgagg gaatcctgtt tctgtctatc
    12961 caagaatggg catgaggtgg caaccgtcgt gctagcgtac agggtgcact ttgtaaccat
    13021 ttgggacacc ggacactcgc tgttttcgaa attacccttt aagcgcgggt attgaaccag
    13081 gcttatgccc aagatcgtag caagcattta actgtgggaa tactcaggta tcgtaagatg
    13141 caagagttcg aatctcttag caaccattat ttttttcctc aacataacga gaacacacag
    13201 gggcgctatc gcacagaatc aaattcgatg actggaaatt ttttgttaat ttcagaggtc
    13261 gcctgacgca tatacctttt tcaactgaaa aattgggaga aaaaggaaag gtgagaggcc
    13321 ggaaccggct tttcatatag aatagagaag cgttcatgac taaatgcttg catcacaata
    13381 cttgaagttg acaatattat ttaaggacct attgtttttt ccaataggtg gttagcaatc
    13441 gtcttacttt ctaacttttc ttacctttta catttcagca atatatatat atatttcaag
    13501 gatataccat tctaatgtct gcccctatgt ctgcccctaa gaagatcgtc gttttgccag
    13561 gtgaccacgt tggtcaagaa atcacagccg aagccattaa ggttcttaaa gctatttctg
    13621 atgttcgttc caatgtcaag ttcgatttcg aaaatcattt aattggtggt gctgctatcg
    13681 atgctacagg tgtcccactt ccagatgagg cgctggaagc ctccaagaag gttgatgccg
    13741 ttttgttagg tgctgtggct ggtcctaaat ggggtaccgg tagtgttaga cctgaacaag
    13801 gtttactaaa aatccgtaaa gaacttcaat tgtacgccaa cttaagacca tgtaactttg
    13861 catccgactc tcttttagac ttatctccaa tcaagccaca atttgctaaa ggtactgact
    13921 tcgttgttgt cagagaatta gtgggaggta tttactttgg taagagaaag gaagacgatg
    13981 gtgatggtgt cgcttgggat agtgaacaat acaccgttcc agaagtgcaa agaatcacaa
    14041 gaatggccgc tttcatggcc ctacaacatg agccaccatt gcctatttgg tccttggata
    14101 aagctaatct tttggcctct tcaagattat ggagaaaaac tgtggaggaa accatcaaga
    14161 acgaattccc tacattgaag gttcaacatc aattgattga ttctgccgcc atgatcctag
    14221 ttaagaaccc aacccaccta aatggtatta taatcaccag caacatgttt ggtgatatca
    14281 tctccgatga agcctccgtt atcccaggtt ccttgggttt gttgccatct gcgtccttgg
    14341 cctctttgcc agacaagaac accgcatttg gtttgtacga accatgccac ggttctgctc
    14401 cagatttgcc aaagaataag gttgacccta tcgccactat cttgtctgct gcaatgatgt
    14461 tgaaattgtc attgaacttg cctgaagaag gtaaggccat tgaagatgca gttaaaaagg
    14521 ttttggatgc aggtatcaga actggtgatt taggtggttc caacagtacc accgaagtcg
    14581 gtgatgctgt cgccgaagaa gttaagaaaa tccttgctta aaaagattct ctttttttat
    14641 gatatttgta cataaacttt ataaatgaaa ttcataatag aaacgacacg aaattacaaa
    14701 atggaatatg ttcatagggt agacgaaact atatacgcaa tctacataca tttatcaaga
    14761 aggagaaaaa ggaggatagt aaaggaatac aggtaagcaa attgatacta atggctcaac
    14821 gaaatgacgg tatcgataag cttgatatcg aattcctgca gcccggggga tccactagtt
    14881 ctagagttaa ttaa
//
