LOCUS       sequence-208217-       18186 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18186)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 18186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU75.2-2.2
FEATURES             Location/Qualifiers
     source          1..18186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2448..2747
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        5365..5585
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             5586..6389
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(14333..14353)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(14333..14351)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(14690..15278)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14770..14789)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15449..16240,16241..16309))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16072..16091
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16310..16414)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      16441..17783
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(17404..17451)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     17824..17842
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(18161..18177)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18161..18177)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atgcttggtg tagcacgcgc cggcgctcgc tctcggcaga
      541 atttatttct ccatcgttcg tcacgaaatc cctggacaat tccttcttca actcggcatg
      601 cccggttcaa ctcaacttcc acggaaattc caccacctaa acctcgatct accattattc
      661 gccgggcatt aaccgttgca aaagtcactg cttatgtcgg tctatcttca gtctttggcc
      721 ttgtcgtcgt aggtgctggg atattcattc acgacgcgtt tacctacaac gaacgacacg
      781 tggaacgggt accagtacat ccattggcgt tgcatccaga gcgaggaggg cccaagaatc
      841 ttccgatagt caaagtgcag atggatgacc aggaggatga ggagacgagg aaattggctg
      901 agaaaccaaa gttggttatc gtcggtggtg gttggggggc tattggaacg ctacgaacgc
      961 ttcatccagg agactaccat gtaaccgtcg tatccgcaga aactttcacc accttcacgc
     1021 cattgctacc ctccgctgct gttggtaccg ttcaagttcg ttcacttatc gagcctattc
     1081 gaaagatcat tgcccgtctg cgcggacatt ttgttgctgg aaaagctgtg gacctttgca
     1141 tcaaggataa actacttgaa gttgaaactt ggagctctaa tggcactaaa gagcgtatat
     1201 atgtccctta cgaccacctc gttatcgcag tcggatcgac ctccagtact cacggtgtcc
     1261 ccggcctcga gaactgtttc cagctcaaga ccatctcaga tgctcaagct atgcggagac
     1321 gcatcatgga caactttgaa atcgctagtc tgccgactac tactcctgaa gagagaaaga
     1381 ggctgttgag ctttgtcgtt tgtggtggtg gtcctactgg cgttgaaaca gccgcggaaa
     1441 tatatgattt ctgtcaagag gatatcatga attacttccc caagatctgc cgagaagagg
     1501 tttccattca tgttatccag tctagagaac atatcctgaa cacttactcg gaagccatat
     1561 caaaatatgc cgaggagaaa tttgaacgcg atggtgtcaa tctcattacg aactcacgcg
     1621 tgagcgccgt aacgcctaca caagtcattt tcacagcgcg agacaatgaa actggtcaaa
     1681 ttactcaaca cacggtccct accaatttcg ttctatggtc gacgggaata gcgatgaatc
     1741 cctttacaaa gagagtcaca gagttgctac ccaatcaagt ccataaaaag gccattgaga
     1801 cggatgcgca tctacgggtg aagggtgcac cggtcggaga gatctatgct gtcggggatt
     1861 gtgctacgat tgaaacttcg ctcgtgagcc acttcatgga attggtggac gaggcagata
     1921 aagacaagaa cggcaagata gatttcggag agtgggaagt aatggtcaag aggatcaaga
     1981 aaaagatacc catggctgag aaccatgtca gcaaagttag agaactcttc gagatgtatg
     2041 acacagattc ggataacagt ttaaccctca acgaactcgt ccggttgttg gaagacctcg
     2101 ggaacaagat caccgcttta cctgctactg cgcaggtagc ttctcaacaa ggcaaataca
     2161 tcgggaagaa gctccacagg ctcgctcggc aaagagagtt ccaacagaca agggcaatgg
     2221 atgttggagg gacgccattc aaatatcacc atctgggcat gttggcatat attggcaacg
     2281 ctgctgtgtt tgatttggga ccttggtcgt tcacgggtgg attggctgcc atgtacgcgt
     2341 ggagaagtgt atattggaat gagcaagtgt cggctaggac tagagcgttg ttgatgattg
     2401 attggattat tcgtggtgtg tggggaagag atttatctag actctaggcg gatctcttat
     2461 gtctttacga tttatagttt tcattatcaa gtatgcctat attagtatat agcatcttta
     2521 gatgacagtg ttcgaagttt cacgaataaa agataatatt ctactttttg ctcccaccgc
     2581 gtttgctagc acgagtgaac accatccctc gcctgtgagt tgtacccatt cctctaaact
     2641 gtagacatgg tagcttcagc agtgttcgtt atgtacggca tcctccaaca aacagtcggt
     2701 tatagtttgt cctgctcctc tgaatcgtct ccctcgatat ttctcatttt ccttcgcatg
     2761 ccagcattga aatgatcgaa gttcaatgat gaaacggtaa ttcttctgtc atttactcat
     2821 ctcatctcat caagttatat aattctatac ggatgtaatt tttcactttt cgtcttgacg
     2881 tccaccctat aatttcaatt attgaaccct cacgatccag ttctccagtg acacagcctt
     2941 tatctggtca aacctttctt tctaatcacc tatgctgatg cttaattaag ggatttttgt
     3001 ctccatcaac ggcatgcgcc caaaaatgac gtttttttta acccatagac acgaaactac
     3061 ccattttcca ccggcctgac ctaccaccgg aacaacggcc atctccaact tgcaagttgg
     3121 ggaaattaag agcatcgcag gtttaatgga agaaaaaaaa aaggtacagc acagcgcaaa
     3181 tggagttagt tcccttatgt cacacactca cacacagtcg gtcagatcaa gcatactggg
     3241 tgcgtataaa tagagtggcc attgccaccc tgtttatctc aaaatctgtc ttgttagtgg
     3301 tcttctccct ttttcaggtt acaattctct tgtttctact tagtatataa gtatatcaag
     3361 ctatattaag catactatca actgtcaact ctatcctcaa aatacaatac aaaatgaacc
     3421 ggacaacaaa aaccttactt accacagctt gcatacttgt atgccttact agaggtgctg
     3481 tatctcaagc tacatggaac gagtcgtcct ggttgtctat cacacctact cagaacttga
     3541 cctgggtgcc atgctacgac ggaggccttg aatgtggtcg cttccaggtc ccattgaatt
     3601 atgccgaccc agaaggacaa tctgctgcca tagcactcgt gcgcatgaaa gccaacgtct
     3661 ctgccgatag tacggattat cttggtccta tcctcttcaa tcccggagga cctggcggta
     3721 gtggtgtcga ctatatagtt tcgttgggca caaaattaag agaaatcttg gggcctcagt
     3781 tcgatttagt gggattcgac cccagaggtg tatcgcgctc cactccacgc gtgacaatct
     3841 atgaaactct tgatgagcgt ctgtcatggc aaaggtctgg aatttgggag ctcaactcct
     3901 ctttgcaaga caatgttgcg agttattggg ctcgtgcaaa gataatcgcc caacttgctg
     3961 aagaacgtca tggggatttc ctgccttata tcaacactga ccacacagca cgagacatgc
     4021 ttcgcattac ggaggcttac ggtagagaga agctccagta ttggggcatc tcatatggca
     4081 cggctcttgg ttcgaccttt gcagctatgt ttccagacaa ggtccaacgc ttggtaatcg
     4141 acggagtgct tgacgttcaa gatgactact acaccatggg atggaaggat gtcgtttccg
     4201 ataccgatgc caccctgaag tggttcttca aagactgcca tgatgcggga gcagaaaaat
     4261 gctccttcta tgagtcgtcg gccgaagcca tcgaagaaag actcaacaga ttataccagt
     4321 ccattatccg agctccggta ccggtacgcg gcgctggcct cattgattat gcatcactgc
     4381 ggttcgtaat attcaatgcg ctgtactatc cattgacccg gtggtccact ttggcaactg
     4441 ccttggccga cttggaaaag ggaaatggta ccacgttggc tcagatgagc gggctagtgc
     4501 catcatcttc ccccgattca gacgacgctc cggtgtcttt tgcaaacaac gatgagtctc
     4561 agactgtgat agtctgcaat gacggtgacg ctgtgccacc tagcctggaa catgcacaac
     4621 agcactatga ggagtccctg aaagtatcgg aatggggtag tatgtcatca gccatgagaa
     4681 ttaaatgcag tggttggccc aacattccaa aggcgccctt cagaggtcct atttcgggaa
     4741 acacgagtca tccgatcctc ctcataggaa atactgctga tcccatcaca cccctctctg
     4801 ctgcacacaa ggtttcaaaa ggtttccccg gatcagtcgt gcttcagcag gattgcgctg
     4861 gccatacttc aatcctggca gggccctcgg tctgcaccta taacataatt cgcgagtatt
     4921 ttgtcaacgg gactctccca gagccaggca ctgtgtgccc tattgaagga tctccgtttt
     4981 aaacaaatcg ctcttaaata tatacctaaa gaacattaaa gctatattat aagcaaagat
     5041 acgtaaattt tgcttatatt attatacaca tatcatattt ctatattttt aagatttggt
     5101 tatataatgt acgtaatgca aaggaaataa attttataca ttattgaaca gcgtccaagt
     5161 aactacatta tgtgcactaa tagtttagcg tcgtgaagac tttattgtgt cgcgaaaagt
     5221 aaaaatttta aaaattagag caccttgaac ttgcgaaaaa ggttctcatc aactgtttaa
     5281 aaggaggata tcaggtccta tttctgacaa acaatataca aatttagttt caaagatgaa
     5341 tcagtgcgcg aaggacataa ctcattcaat tcaattcatc attttttttt tattcttttt
     5401 tttgatttcg gtttctttga aatttttttg attcggtaat ctccgaacag aaggaagaac
     5461 gaaggaagga gcacagactt agattggtat atatacgcat atgtagtgtt gaagaaacat
     5521 gaaattgccc agtattctta acccaactgc acagaacaaa aacctgcagg aaacgaagat
     5581 aaatcatgtc gaaagctaca tataaggaac gtgctgctac tcatcctagt cctgttgctg
     5641 ccaagctatt taatatcatg cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc
     5701 gtaccaccaa ggaattactg gagttagttg aagcattagg tcccaaaatt tgtttactaa
     5761 aaacacatgt ggatatcttg actgattttt ccatggaggg cacagttaag ccgctaaagg
     5821 cattatccgc caagtacaat tttttactct tcgaagacag aaaatttgct gacattggta
     5881 atacagtcaa attgcagtac tctgcgggtg tatacagaat agcagaatgg gcagacatta
     5941 cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag gcggcagaag
     6001 aagtaacaaa ggaacctaga ggccttttga tgttagcaga attgtcatgc aagggctccc
     6061 tatctactgg agaatatact aagggtactg ttgacattgc gaagagcgac aaagattttg
     6121 ttatcggctt tattgctcaa agagacatgg gtggaagaga tgaaggttac gattggttga
     6181 ttatgacacc cggtgtgggt ttagatgaca agggagacgc attgggtcaa cagtatagaa
     6241 ccgtggatga tgtggtctct acaggatctg acattattat tgttggaaga ggactatttg
     6301 caaagggaag ggatgctaag gtagagggtg aacgttacag aaaagcaggc tgggaagcat
     6361 atttgagaag atgcggccag caaaactaaa aaactgtatt ataagtaaat gcatgtatac
     6421 taaactcaca aattagagct tcaatttaat tatatcagtt attaccctat gcggtgtgaa
     6481 ataccgcaca gatgcgtaag gagaaaatac cgcatcagga taggaaaaaa ccgagcttcc
     6541 tttcatccgg cgcggctgtg ttctacatat cactgaagct ccgggtattt taagttatac
     6601 aagggaaaga tgccggctag actagcaagt tttaggctgc ttaacattat ggataggcgg
     6661 ataaagggcc caaacaggat tgtaaagctt agacgcttct ggttggacaa tggtacgttt
     6721 gtgtattaag taaggcttgg ctggggatag caacattggg cagagtatag aagaccacaa
     6781 aaaaaaggta tataagggca gagaagtctt tgtaatgtgt gtaacttctc ttccatgtgt
     6841 aatcagtatt tctacttact tcttaaatat acagaagtaa gacagataac caacagcctt
     6901 tcccagatat acatatatat ctttatttca gcttaaacaa taattatatt tgtttaactc
     6961 aaaaataaaa aaaaaaaacc aaactcacgc aactaattat tccataataa aataacaaca
     7021 tggaatcagt cagtcacctc ccgtatttct tccccgacgc acagcaacag cccgagccat
     7081 cgcagccgaa caccagtcgt cgtggctctg ttctctcttc cattctctcc ggaagcggtt
     7141 gcgtatccac ttcgtctgcc tccattcctc catcggcatc tgggcccaaa tgtggtgtga
     7201 agcgatcccg tgatgaaacc aaggacggac agcctccatc tcgagacagt tatctggtca
     7261 ttccagagcc caagcgccgt agaatccaga cagatacaga tttcccgctt ccattttcta
     7321 ccacatcggc acacacagag ggaaagccca caagcacaac tacctctccc ccagccacac
     7381 ctccagagct tccttgccgt gactgggcaa gcaaggttgc actgggcctc tggattggtg
     7441 aatatctgtg tgacagattt tctacgcctg agttcaggtt tgtcgaaaat catattatca
     7501 tgaaggaaat gagcgggaaa ctcgttagcg tcttggacta cctcgacccg cacaactcga
     7561 tcgctcttgc cgctcttatc tacctggaga ggctcattcg tggcatcaaa atcggcgaaa
     7621 catttagtga tcgcgatcgc gctaccatca tgtataggtc atacctactc gctctccact
     7681 ttgcctttat ctggctagaa gatattcctt ggtccctctc aaagatcgct gaatgcatgg
     7741 aagtctctct ggaagacgcg agattgtggg aacaccaggg cctctcattg ctagactaca
     7801 atctcttcat tgcagaaggc atttggcccg catggctgga catgcttcat aaacatgccg
     7861 cattcacgga atgtcatgac cacattggcc ttacgttaca agatatcttc cttacagcca
     7921 aggatgaata cagggcatct gtccagcaca tccaagcacc ttctccggca tcgtcaccat
     7981 cacttccatc accacctcac accccgccgc cgtctgctct tgctccaaag gtagcaacga
     8041 ctactgttcg tgagttacca accccgcacc ctggccgttc gtcatcgctc gtcaagtcga
     8101 aggaagattg ggatagtggt tttgagtttt ccactgccag ccttgagcat gcttctgctc
     8161 cagaccgaca gccgtctctg ttcgacgcca ctcttcctat tctgcagccc aaacctgttc
     8221 ttcctgccca ccaacacgtt gatagcttgc tcatggcggt tgcctagagt gcttttaact
     8281 aagaattatt agtcttttct gcttattttt tcatcatagt ttagaacact ttatattaac
     8341 gaatagttta tgaatctatt taggtttaaa aattgataca gttttataag ttactttttc
     8401 aaagactcgt gctgtctatt gcataatgca ctggaagggg aaaaaaaagg tgcacacgcg
     8461 tggctttttc ttgaatttgc agtttgaaaa ataactacat ggatgataag aaaacatgga
     8521 gtacagtcac tttgagaacc ttcaatcagc tggtaacgtc ttcgttaatt ggatactcaa
     8581 aaaagatgga tagcatgaat cacaagatgg aaggaaatgc gggccacgac cacagtgata
     8641 tgcatatggg agatggagat gatacctcca ttgggccgat gaagttagtc gacggataga
     8701 agcggttgtc ccctttcccg gcgagccggc agtcgggccg aggttcggat aaattttgta
     8761 ttgtgttttg attctgtcat gagtattact tatgttctct ttaggtaacc ccaggttaat
     8821 caatcacagt ttcataccgg ctagtattca aattatgact tttcttctgc agtgtcagcc
     8881 ttacgacgat tatctatgag ctttgaatat agtttgccgt gattcgtatc tttaattgga
     8941 taataaaatg cgaaggatcg atgaccctta ttattatttt tctacactgg ctaccgattt
     9001 aactcatctt cttgaaagta tataagtaac agtaaaatat accgtacttc tgctaatgtt
     9061 atttgtccct tatttttctt ttcttgtctt atgctatagt acctaagaat aacgactatt
     9121 gttttgaact aaacaaagta gtaaaagcac ataaaagaat taagaaaatg gaacaacctg
     9181 caaaacgtcg tcgcattcag cgctccccct ctccgacata taggctcgac gacgatagta
     9241 attacgaacc gtatatcccg atagcccaaa gacgacaagc aaagctcgca aaactctcat
     9301 ctctgggtgc ttcgacggag agagaccgga atagcaagca gagagaggag gaggaacgcg
     9361 aagaagagaa ccaggaagaa gaacgacaaa gagaaaagtc gaggaaggag agaaccttac
     9421 tactagaagc tcaagaggtg cattccaaga aggccgctga agacgcgaag aagactcagg
     9481 aggaaaaggc acgggaagtg gatgaagaga tcctggaggc tataaagatt cgcaggaagc
     9541 tagcttcaga cagagagctg gccaaaggca tacaatatac cgaatcttta aaaactagct
     9601 ggcgaccccc aagatatatt cgcgaaaaga caaaggaaca gcacgaacag cttcgcgaga
     9661 aataccatat cattgtagaa ggagaagatg ttccgcctcc aatcgaacac ttcagcgaca
     9721 tgaagatacc cgactccatt ttgcagcatc tcaagtcgaa gcgcattaca aaaccgacac
     9781 caattcagct tcagggtatt ccgacggcct tcgctgggag agatctcatc ggaatagcat
     9841 tcacaggttc agggaaaacg ctagctttct gcatgcccct gatcatgctc gcaatcgagg
     9901 aagaagccaa acttcctctc gttcgaggcg aagggcccgt cggtgtcatc ctatgtccat
     9961 cccgagaact agcaaaccaa acctacgaaa acgtgctcac atggaccgac gctctcgctc
    10021 aggacggcaa atatccacgg ctgaataccc tgttatgtat aggtggcatt tccatgaatg
    10081 aacaaagcca tacattgagc aaaggtctgc acattgtggt ggctactcct ggaaggctta
    10141 tcgacatgct ggaaaagaag cgttttacat ttaacaactg caaatatctt tgcatggacg
    10201 aagccgaccg gatgattgat ctgggttttg aagatgatgt gcggaatatt atgagctttt
    10261 tcaagcgtca acgacaaaca ttactcttct ccgcaacgat gcctcggaag atccaggatt
    10321 ttgcgcaaca atccctcatc aaaccggttc tcgtcaatgt aggccgtgct ggtgctgcca
    10381 acttggatgt cctgcaagtc gtcgaatacg tcaagcaaga agccaagatg gtgtacttgt
    10441 tggagtgcct acaaaaaaca cctccaccgg taatcatctt cagtgagaac aagaatgagg
    10501 tcgacgatat tcaagagtat ctattgctca aaggtgttga ggccgtagcg atccatggtt
    10561 ccaagagcca agaagagcgt caatacgcta tcaagtcctt caagtcgggt gcaaaagacg
    10621 tcatggtagc atccggcgtc gcctcaaaag gactcgactt caacgatatt cagcatgtca
    10681 tcatcttttc gatgcctaaa gaaattgaag actacgtgca tcaaatcgga cgtacaggtc
    10741 gtagtggaaa gacgggtatt gcaacgacat ttgtgaatat gaatacaccg gagcagacgt
    10801 tgttggatct caagtatttg cttatggagg cggggcaaaa agttcctccg ttccttgcta
    10861 ctattgagga tcctcgtgct gctcaaggcg gatcgttgaa gggatgcccc gtttgtggtg
    10921 gcctgggcca tgggattagc aattgtccca agcttgagga gacacagagg aggcagatgg
    10981 cgtcgcatcg tacgactgat gatgggggct actgaattta actccttaag ttactttaat
    11041 gatttagttt ttattattaa taattcatgc tcatgacatc tcatatacac gtttataaaa
    11101 cttaaataga ttgaaaatgt attaaagatt cctcagggat tcgatttttt tggaagtttt
    11161 tgtttttttt tccttgagat gctgtagtat ttgggaacaa ttatacaatc gaaagatata
    11221 tgcttacatt cgaccgtttt agccgtgatc attatcctat agtaacataa cctgaagcat
    11281 aactgacact actatcatca atacttgtca catgagaact ctgtgaataa ttaggccact
    11341 gaaatttgat gcctgaagga ccggcatcac ggattttcga taaagcactt agtatcacac
    11401 taattggctt ttcgctagtc ttatctaaaa attgccttta tagtccgtct ctccagtcac
    11461 ggcctgtgta actgattaat cctgcctttc taatcaccat tctactgttt aattaaggga
    11521 ttttgtcttc atcaacggct tccgcccaaa aaaaagtatg acgttttgcc cgcaggcgtg
    11581 aagctgccca tcttcacggg cctgacctcc tctgccggaa caccggccat ctccaactca
    11641 taaattggag aaataagaga atttcagatt ttcagaggat gaaaaaaaaa aggtagagag
    11701 cataaaaatg gggttcactt tttggcaaag ttacagtatg cttattacat ataaatagag
    11761 tgccgataat ggcttttttt catcttcgaa atacgcttgc tactgctctt ccagcgtttt
    11821 tattacttct ttcttgtttc tccttagtat ataaaatatc aagctacaac aagcatacaa
    11881 tcaactgtca actgtcaatt atattataat acactatgtc tgttgcaatg ccggatatac
    11941 ccgctgaggc ctattatatt tacggtcaca agagatatct cgttgcgctt ggactcgcgc
    12001 tgaccctctt tatctctgcg ttggactcaa ctatcgtcgc ggtcgcgtta cccacgattg
    12061 gcgcggactt taatgagtat cagcaaacca gttggctcgt tacctcctac ctgttgacct
    12121 acacggcgtt tcttccagta gtctccaagc tcacagatat cgtggggaga aagccagttc
    12181 tgattgtatc cactctgttc tttatgttat ggtccggagc gtgtggaggg gccaagtcaa
    12241 tgacacagct cattgtgttt agggcgttgc agggaatcgg aggttcagca atctactctg
    12301 gcgttgtggt gacgatcagt acgattgttc ctagggagaa agttggtgca tacacaccca
    12361 tcattgggac agttttcgcc gcaagcagtg tgtcaggtcc actcattggt ggtgcaatcg
    12421 ttcagcacgt ccattgggga tggattttct tcgtcaactt acccatcgga gctgttgcat
    12481 catctttgtt gttctacgct ctgagtgatc cagatctcgg ccctatgagc atccaaaaga
    12541 tcagtcaaag gatagattgg ataggcagtt tcctgttact ggctgccagt gttctcctcg
    12601 cgttctcgct ccaagtcgga ggtacagacg gatatccatg ggtatccgtc caagtactcg
    12661 ccccgctcat catctccttc tgcattctac cagtcttcgc gttcgtcgaa actcgtcacc
    12721 cagaacccgt tattccttta cgtctattcc gagtccgcaa catctgcttt attttgattt
    12781 tcacgctggc attaggagcc ggtctgttct cgcatgccat cttccttccg cagcgcatgc
    12841 aggtcgtaga tggtgtttca cctgtcaccg ccggtgttcg catgctgccc cttctccttc
    12901 ccgttggatg cctcagtccc ttcgcaggcg ccatggtcat gctgaccaaa agttaccgac
    12961 ctctcatgtg gttcgcctcg tccatcggtg ctatcggtgc tggcctattg tccactgtca
    13021 ccgtcccaac caagttttcc catatttacg gattcgaagc catggttggc ttttctatcg
    13081 gtgtcaccat caccgcgtcg accttgatca tccagttctg cgccgagaga cgagatttgg
    13141 cggctgctac gggcctccag acgttcattc ggcaattggg tggattggtt gcaattgcaa
    13201 tttcgacggc gattctaaat gattcggtgg aaaagtctct cggcagtaat gcgcgattgg
    13261 ccagttctcc aggattgctt gaaggcattt tgaagagccc gactagtgta ttaccttcgc
    13321 tggatccggc aacgagcctc atcatacgac aagcgtatag caaaggtttc tctgtaacgt
    13381 ttatccaggc cagtgcttgg ttggctgtcg gagctcttgc cacttttggg cttgtacatt
    13441 atctgcctga taggtttttg gacgacgagc agaccaaggg agaggttctt gatcctgaga
    13501 aggctggtgc aggctcgatt gacaatacca tcaagactgg agaaacatct ggagttgcga
    13561 acgaaccgga tgagccgcta ggtaaagagc cggcaaaggt tgaagtggtt gatgctgaga
    13621 aaactgtagt aaactcgggt ggagttgcgc acaacgaacc agatgaaccg ctaggtaaag
    13681 agctggcaaa ggttcaagtg gctgatgctg agaaagctgt agtagactcg ggtgaaaagg
    13741 ccgctgagac aggggaaagg aacgagacgg aggagaccac atcccaaaag ttgtaaggag
    13801 attgataaga cttttctagt tgcatatctt ttatatttaa atcttatcta ttagttaatt
    13861 ttttgtaatt tatccttata tatagtctgg ttattctaaa atatcatttc agtatctaaa
    13921 aattcccctc ttttttcagt tatatcttaa caggcgacag tccaaatgtt gatttatccc
    13981 agtccgattc atcagggttg tgaagcattt tgtcaatggt cgaaatcaca tcagtaatag
    14041 tgcctcttac ttgcctcata gaatttcttt ctcttaacgt caccgtttgg tcttttatag
    14101 tttcgaaatc tatggtgata ccaaatggtg ttcccaattc atcgttacgg gcgtattttt
    14161 taccaattga agtattggaa tcgtcaattt taaagtatat ctctctttta cgtaaagcct
    14221 gcgagatcct cttaagtata gcggggaagc catcgttatt cgatattgtc gtaacaaata
    14281 ctttgatcgg cgctatgcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
    14341 gagggttaat tgcgcgcttg gcgagactca aacaagatat attttgcccg ccttacagac
    14401 gaaactagtt ggaggttatg gagcatacta tcacgtgggc ggccactggt gagttactac
    14461 accccagggg caacgttgat gctcctaaaa aactctggct ggacgcaagc cgtaacaccc
    14521 gtgtcacttc ataatcgttt gcaattcagg gcttgatcta cacattaccc tgttatccct
    14581 attaattaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    14641 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    14701 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    14761 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    14821 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    14881 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    14941 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    15001 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    15061 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    15121 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    15181 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    15241 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    15301 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    15361 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    15421 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    15481 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    15541 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    15601 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    15661 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    15721 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    15781 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    15841 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    15901 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    15961 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    16021 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    16081 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    16141 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    16201 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    16261 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    16321 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    16381 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    16441 aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt
    16501 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt
    16561 taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa
    16621 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc
    16681 tattttacca acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc
    16741 gctatttttc taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg
    16801 cagtctcttg ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg
    16861 tgtctatttt ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta
    16921 gcgaagctgc gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat
    16981 gtggattgcg catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag
    17041 aaaattatga acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca
    17101 ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa
    17161 gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg
    17221 agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga
    17281 tacttttgag caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc
    17341 cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc
    17401 tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaactt caaagcgttt
    17461 ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc acatacagct cactgttcac
    17521 gtcgcaccta tatctgcgtg ttgcctgtat atatatatac atgagaagaa cggcatagtg
    17581 cgtgtttatg cttaaatgcg tacttatatg cgtctattta tgtaggatga aaggtagtct
    17641 agtacctcct gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta
    17701 ccctttagct gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct
    17761 atcatttcct ttgatattgg atcatactaa gaaaccatta ttatcatgac attaacctat
    17821 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
    17881 ctcggcgcgc cattaccctg ttatccctaa tatacaacgc aaagaagctg gaaaattggc
    17941 gagggaatcc tgtttctgtc tatccaagaa tgggcatgag gtggcaaccg tcgtgctagc
    18001 gtacagggtg cactttgtaa ccatttggga caccggacac tcgctgtttt cgaaattacc
    18061 ctttaagcgc gggtattgaa ccaggcttat gcccaagatc gtagcaagca tttaaatgac
    18121 ggtatcgata agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagtt
    18181 aattaa
//
