LOCUS       sequence-208220-       10437 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ag43 under the control of a blue-light transcriptional 
            regulator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Jin X, Riedel-Kruse IH
  TITLE     Biofilm Lithography enables high-resolution cell patterning via 
            optogenetic adhesin expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Apr 3;115(14):3698-3703. doi: 
            10.1073/pnas.1720676115. Epub 2018 Mar 19.
  PUBMED    29555779
REFERENCE   2  (bases 1 to 10437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1720676115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-04-3- 3"; volume: "115"; issue: "14"; pages: "3698-3703"
COMMENT     Sequence Label: pDawn-Ag43
FEATURES             Location/Qualifiers
     source          1..10437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(26..73)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     69..87
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(140..157)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(3312..3344)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(3348..3365)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(3375..3392)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             3411..3433
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      complement(3509..3536)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(3552..3623)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3671..3703)
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     CDS             complement(3704..4414)
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=lambda repressor
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     RBS             4421..4432
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(4755..4774)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        4980..5057
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             7615..7806
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(7800..7822)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7908..8047
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7963..7982
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8062..8079)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8233..8821)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8313..8332)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             9056..9847
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     terminator      9859..9953
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      complement(9971..10426)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10108..10129)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10320..10339
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtcagaag gtcacattca
      181 gtgtggcctg accgttatac ccttcagcgc tgctgccgct gacgctgtgg gcataaccgg
      241 cctgaacgcc cagggtgata ttttcccgga cacgggcttc cagtccggct tgcaggtcca
      301 gtgatgtgcc attctgtgag ggagagaacg tcatcccgct gcttgcagtg gaagtcccca
      361 cacgcatatc tccccgggag ctgaaggtgc ggataacaga aggctgtacc caccagttca
      421 ccggtaattc actcacactg tgttttgcac tgtcacgcag gggggcacgg gatgaggtgc
      481 cttcgccaaa ggtcatatcg ttgtggctgc ccagacggaa accggcacgc acatgttgtg
      541 cactgccatg cccgaacttc acataaccgg cgttgtcctt accgtcatcc agggaaagtc
      601 cctgccaggt atattgcagt tgtggctcca gcatcaggtt gtcagtgata ctgaagggca
      661 gaccggtttc cagtgagccc agccagcccc agccccgggc gcggaagtcg ttattgtccg
      721 atgacgcttt catgctgtgg cgggttccct gtgccacaat gtcagcccac aggccggagg
      781 acgtgtgtac cagattcagg tatccgccca ggctgccggc atcatcccgg accgtgccgg
      841 cacgggagcc gtcatcatcc ttaacatcaa cggaagaatg gccagcagca ccatataccc
      901 ccgcggtcac agacataccg gcaacctctg ttctcatcag gtcaccctcc agacggacga
      961 atccatagct gccgctgctt tccggcgtgg ccccacgggc aataccgcca ttgttatcgt
     1021 gaccgagatg accgccctga atgctgagac ggacgctgtt gttttcacca tttacaccgg
     1081 tctgatggct gcgggagcct gccacaatcc ggtcatagtc cattgcctgt gtcagcatgg
     1141 aggcatacag ggggacttct gcacgataag cattttcact gcgcagatac cagctctcat
     1201 cactgtcccg gttgagggag tagttaaagg caccggcttg cagcctgttc ccctggacaa
     1261 aggccccttc ctccgtggtg gcaccgttaa tggcttccac cacctgaata cccttaccgc
     1321 tggtcgccag ccccgacgca ctgttgccgg cgttcaccag gttcaggatg gtttttccgg
     1381 ttgccctgcc gccgtcaatg accagtctgt cagcattgtt ctgtgccata tccgggcgta
     1441 cacgcaggct gatggtgcca ttctgtccgt tcaggttttt cactttcagg gttgccggta
     1501 cgaacttccc tgtgcgggtg gaggtgaaat gaatctgtcc ggcatggctg aggtcatcca
     1561 ccaccgactg caccgtggcg ttatcgggga tattccaggt ggcaccggag gcgagagtga
     1621 cattcgtggg gtcaatggca ccgttcagca cagtgctgcc ggtcagcttc agggctgtac
     1681 cgcgctgagc aatgacatcc gtggtgacgg tactgtcgtt cagcgtcagc gtgccttcat
     1741 tcaggttgac ggctttctgg gtgagtgtgg tgttgctgac agtgagtgtg ccacttcctg
     1801 atttttccac gctgccgtta ccggtgagag agcctccttg caggagtgca tcgccttcac
     1861 ggatggtcag ggtatcgttg ttcaccgtct tcccggaaag ggtaagtatg gcgccgttat
     1921 tcagcgtggt ggtgcccgcc agtgtgccgc ccctggcggt gaacagtccg ccatttaccg
     1981 tggtatccgt ggccgtatca ccggcgttca gcgtgaatga actgcctttt tccagcatca
     2041 gggcatccgc ctgaccgcct ccgatactga atgcctttcc gtcgctccgg gtaccactga
     2101 cagcggcacc ggaatcggcc agcagtacac cgccatttcc catggatacg gtggtggcgg
     2161 tgccaccgtt gcggacatcc agcgttccgc catcatccac gcgggtatta gtggctgtgt
     2221 gtccggtcag cacatccagg cgtccgccat tttccagtac gacattatca gctttaccct
     2281 ccacaacaga gaatgctccc aggcggttta tgccggtaac ggttgcagcc gtactggtaa
     2341 ccagtgcgcc gccctgcttc agggtgacat tcgtggctgt accaccggcg tccacttgca
     2401 gtctgccctt ctgattaacg gtggtattcc cggccacacc cccgtttttg acaatctgcc
     2461 agccgtcact gttcacaaca gtgtcagacg ctgtaccgcc gttgtgcaca tactggtatc
     2521 ccccattcag cgtggtatcc agtgcgtgac catgtacagt ctggtcgccg ccggcataaa
     2581 ccacagtggt atttgccgtt ccttcagctc tcacaatctg gcgaccgttt ttattgatgg
     2641 ttgtgcgtac ggcatccccg cgaacaaact gcccggtatc accgttttct gcatccggtc
     2701 ccccttccgc cccggtatta acaacggtat ccgttgccac tgtaccgggc ttgacgacct
     2761 gccagccctt atcattaatg acggttcctg tggctatcgc cccctcatgc atccactgtt
     2821 cgccaccatt cagcgtggtg ttcacagccc gtccctgaag gctctgtccg cctccggcac
     2881 tgataaccgt gtctgagaca cttccaccgg ggttcactct ctgaagacca ccactggtga
     2941 cagtcgtttt gttggctgtt ccgccatcct gtacccattg cccgccggta ttggcctcgt
     3001 tatccggccc atactccagc ccggtactga tggtcattcc gttggtcgta ccgaagacaa
     3061 tctggttgtc atgatttgcc agtgttccgc cgttcacggt ttctcccggg tgcacaacga
     3121 tgtcagcagc cagcaccggg agtgacgtga ctgcggcaag agacagtgca accgccacac
     3181 cgccacgttt accccgtgcg cgggccagtt cggaggcaac cacgaaagcg cccgtcatgt
     3241 gattccatac cagcctgtag caggtattca gatgtcgttt cattctagta ggtttcctgt
     3301 gtgaggatcc gacccatttg ctgtccacca gtcatgctag ccatatggct gccgcgcggc
     3361 accaggccgc tgctgtgatg atgatgatga tggctgctgc ccatggtata tctccttctt
     3421 aaagttaaac aaaattattt ctagagcaac cattatcacc gccagaggta aaatagtcaa
     3481 cacgcacggt gttactctag tatataaacg cagaaaggcc cacccgaagg tgagccagtg
     3541 tgactctagt agagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc
     3601 gactgagcct ttcgttttat ttgatgcctg gctctagtag cgatctacac tagcactatc
     3661 agcgttatta agctactaaa gcgtagtttt cgtcgtttgc agcgccaaac gtctcttcag
     3721 gccactgact agcgataact ttccccacaa cggaacaact ctcattgcat gggatcattg
     3781 ggtactgtgg gtttagtggt tgtaaaaaca cctgaccgct atccctgatc agtttcttga
     3841 aggtaaactc atcaccccca agtctggcta tgcagaaatc acctggctca acagcctgct
     3901 cagggtcaac gagaattaac attccgtcag gaaagctcgg cttggagcct gttggtgcgg
     3961 tcatggaatt accttcaacc tcaagccaga atgcagaatc actggctttt ttggttgtgc
     4021 ttacccatct ctccgcatca cctttggtaa aggttctaag ctcaggtgag aacatccctg
     4081 cctgaacatg agaaaaaaca gggtactcat actcacttct aagtgacggc tgcatactaa
     4141 ccgcttcata catctcgtag atttctctgg cgattgaagg gctaaattct tcaacgctaa
     4201 ctttgagaat ttttgcaagc aatgcggcgt tataagcatt taatgcattg atgccattaa
     4261 ataaagcacc aacgcctgac tgccccatcc ccatcttgtc tgcgacagat tcctgggata
     4321 agccaagttc atttttcttt ttttcataaa ttgctttaag gcgacgtgcg tcctcaagct
     4381 gctcttgtgt taatggtttc ttttttgtgc tcatctagta tttctcctct tttctagact
     4441 ccgttgtgat gacgcattgg tacgcggtat cgggaggttc gaaaatttcg agcgatatct
     4501 taaggggggt gccttacgta gaaccccgta ggtcatgccc gaggccggtc ctggatggcg
     4561 cggcggatac gcttgagcag gttttcgtcg agaagcggct tcaaaaccac gtcttttacg
     4621 ccggcctcgg cggcccgggt cgagatgttt tcgtccggat agccggtgat caggatcacg
     4681 ggcgtagatc tcgatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
     4741 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
     4801 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
     4861 gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
     4921 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gagatcccgg
     4981 acaccatcga atggtgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc
     5041 aattgagggt ggtgaatgtg gctagttttc aatcatttgg gataccagga cagctggaag
     5101 tcatcaaaaa agcacttgat cacgtgcgag tcggtgtggt aattacagat cccgcacttg
     5161 aagataatcc tattgtctac gtaaatcaag gctttgttca aatgaccggc tacgagaccg
     5221 aggaaatttt aggaaagaac tgtcgcttct tacaggggaa acacacagat cctgcagaag
     5281 tggacaacat cagaaccgct ttacaaaata aagaaccggt caccgttcag atccaaaact
     5341 acaaaaaaga cggaacgatg ttctggaatg aattaaatat tgatccaatg gaaatagagg
     5401 ataaaacgta ttttgtcgga attcagaatg atatcaccga gcaccagcag acccaggcgc
     5461 gcctccagga actgcaatcc gagctcgtcc acgtctccag gctgagcgcc atgggcgaaa
     5521 tggcgtccgc gctcgcgcac gagctcaacc agccgctggc ggcgatcagc aactacatga
     5581 agggctcgcg gcggctgctt gccggcagca gtgatccgaa cacaccgaag gtcgaaagcg
     5641 ccctggaccg cgccgccgag caggcgctgc gcgccggcca gatcatccgg cgcctgcgcg
     5701 acttcgttgc ccgcggcgaa tcggagaagc gggtcgagag tctctccaag ctgatcgagg
     5761 aggccggcgc gctcgggctt gccggcgcgc gcgagcagaa cgtgcagctc cgcttcagtc
     5821 tcgatccggg cgccgatctc gttctcgccg accgggtgca gatccagcag gtcctggtca
     5881 acctgttccg caacgcgctg gaagcgatgg ctcagtcgca gcgacgcgag ctcgtcgtca
     5941 ccaacacccc cgccgccgac gacatgatcg aggtcgaagt gtccgacacc ggcagcggtt
     6001 tccaggacga cgtcattccg aacctgtttc agactttctt caccaccaag gacaccggca
     6061 tgggcgtggg actgtccatc agccgctcga tcatcgaagc tcacggcggg cgcatgtggg
     6121 ccgagagcaa cgcatcgggc ggggcgacct tccgcttcac cctcccggca gccgacgaga
     6181 tgataggagg tctagcatga cgaccaaggg acatatctac gtcatcgacg acgacgcggc
     6241 gatgcgggat tcgctgaatt tcctgctgga ttctgccggc ttcggcgtca cgctgtttga
     6301 cgacgcgcaa gcctttctcg acgccctgcc gggtctctcc ttcggctgcg tcgtctccga
     6361 cgtgcgcatg ccgggccttg acggcatcga gctgttgaag cggatgaagg cgcagcaaag
     6421 cccctttccg atcctcatca tgaccggtca cggcgacgtg ccgctcgcgg tcgaggcgat
     6481 gaagttaggg gcggtggact ttctggaaaa gcctttcgag gacgaccgcc tcaccgccat
     6541 gatcgaatcg gcgatccgcc aggccgagcc ggccgccaag agcgaggccg tcgcgcagga
     6601 tatcgccgcc cgcgtcgcct cgttgagccc cagggagcgc caggtcatgg aagggctgat
     6661 cgccggcctt tccaacaagc tgatcgcccg cgagtacgac atcagcccgc gcaccatcga
     6721 ggtgtatcgg gccaacgtca tgaccaagat gcaggccaac agcctttcgg agctggttcg
     6781 cctcgcgatg cgcgccggca tgctcaacga ttgacaattg atgtaagtta gctcactcat
     6841 taggcaccgg gatctcgacc gatgcccttg agagccttca acccagtcag ctccttccgg
     6901 tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc
     6961 gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc
     7021 gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc
     7081 ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg
     7141 gcggccccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
     7201 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
     7261 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
     7321 taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     7381 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     7441 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     7501 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     7561 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     7621 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     7681 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     7741 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     7801 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     7861 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     7921 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     7981 tactgagagt gcaccattgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     8041 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8101 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8161 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8221 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8281 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8341 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8401 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8461 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8521 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8581 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8641 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     8701 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8761 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8821 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8881 agggattttg gtcatgaaca ataaaaccgc aagcgaaccg gaattgccag ctggggcgcc
     8941 ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat
     9001 ctgatggcgc aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgtatgag
     9061 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     9121 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     9181 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     9241 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     9301 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     9361 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     9421 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     9481 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     9541 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     9601 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     9661 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     9721 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     9781 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     9841 caaataagag ctcgcttgga ctcctgttga tagatccagt aatgacctca gaactccatc
     9901 tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccaagca
     9961 gtgccacctg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
    10021 atcagctcat tttttaacca atagaccgaa atcggcaaaa tcccttataa atcaaaagaa
    10081 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
    10141 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
    10201 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
    10261 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
    10321 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
    10381 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgcca
//
