LOCUS       sequence-208234-       14203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  p35S:CAS9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14203)
  TITLE     pJKW0457
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJKW0458
FEATURES             Location/Qualifiers
     source          1..14203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2306..2651
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2594..2614
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             2663..2728
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2735..2755
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2780..6880
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2780..6880
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6881..6928
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      6973..7680
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(7705..7722)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7705..7721)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7714..7736)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    7924..7948
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9249..9878
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10307..11380
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11446..11640
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11876..11898)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11984..12124
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12039..12058
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12139..12156)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12310..12898)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12390..12409)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12985..13779)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
     1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
     1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
     1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
     1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
     1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
     1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt
     1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac
     1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
     1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga
     1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat
     1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat
     2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt
     2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac
     2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga
     2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata
     2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc
     2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg
     2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     2641 tggagaggac acgctcgaga tggactataa ggaccacgac ggagactaca aggatcatga
     2701 tattgattac aaagacgatg acgataagat ggccccaaag aagaagcgga aggtcggtat
     2761 ccacggagtc ccagcagccg acaagaagta cagcatcggc ctggacatcg gcaccaactc
     2821 tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct
     2881 gggcaacacc gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag
     2941 cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg
     3001 gaagaaccgg atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga
     3061 cagcttcttc cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg
     3121 gcaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat
     3181 ctaccacctg agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta
     3241 tctggccctg gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa
     3301 ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct
     3361 gttcgaggaa aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag
     3421 actgagcaag agcagacggc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa
     3481 tggcctgttc ggaaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa
     3541 cttcgacctg gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct
     3601 ggacaacctg ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa
     3661 cctgtccgac gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc
     3721 ccccctgagc gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct
     3781 gaaagctctc gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag
     3841 caagaacggc tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt
     3901 catcaagccc atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaacag
     3961 agaggacctg ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca
     4021 cctgggagag ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga
     4081 caaccgggaa aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct
     4141 ggccagggga aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc
     4201 ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat
     4261 gaccaacttc gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta
     4321 cgagtacttc accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag
     4381 aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac
     4441 caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt
     4501 cgactccgtg gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca
     4561 cgatctgctg aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat
     4621 tctggaagat atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg
     4681 gctgaaaacc tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag
     4741 atacaccggc tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc
     4801 cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca
     4861 gctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg
     4921 ccagggcgat agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa
     4981 gggcatcctg cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa
     5041 gcccgagaac atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa
     5101 gaacagccgc gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat
     5161 cctgaaagaa caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta
     5221 cctgcagaat gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga
     5281 ctacgatgtg gaccatatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa
     5341 ggtgctgacc agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt
     5401 cgtgaagaag atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag
     5461 aaagttcgac aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg
     5521 cttcatcaag agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct
     5581 ggactcccgg atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt
     5641 gatcaccctg aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt
     5701 gcgcgagatc aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac
     5761 cgccctgatc aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt
     5821 gtacgacgtg cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa
     5881 gtacttcttc tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg
     5941 cgagatccgg aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga
     6001 taagggccgg gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt
     6061 gaaaaagacc gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa
     6121 cagcgataag ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga
     6181 cagccccacc gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa
     6241 gaaactgaag agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga
     6301 gaagaatccc atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat
     6361 catcaagctg cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc
     6421 ctctgccggc gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt
     6481 cctgtacctg gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa
     6541 acagctgttt gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga
     6601 gttctccaag agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa
     6661 caagcaccgg gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct
     6721 gaccaatctg ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag
     6781 gtacaccagc accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct
     6841 gtacgagaca cggatcgacc tgtctcagct gggaggcgac aaaaggccgg cggccacgaa
     6901 aaaggccggc caggcaaaaa agaaaaagta atctagagag ttaattaaga cccgggacta
     6961 gtccctagag tcctgcttta atgagatatg cgagacgcct atgatcgcat gatatttgct
     7021 ttcaattctg ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga tccttaccgc
     7081 cggtttcggt tcattctaat gaatatatca cccgttacta tcgtattttt atgaataata
     7141 ttctccgttc aatttactga ttgtacccta ctacttatat gtacaatatt aaaatgaaaa
     7201 caatatattg tgctgaatag gtttatagcg acatctatga tagagcgcca caataacaaa
     7261 caattgcgtt ttattattac aaatccaatt ttaaaaaaag cggcagaacc ggtcaaacct
     7321 aaaagactga ttacataaat cttattcaaa tttcaaaagt gccccagggg ctagtatcta
     7381 cgacacaccg agcggcgaac taataacgct cactgaaggg aactccggtt ccccgccggc
     7441 gcgcatgggt gagattcctt gaagttgagt attggccgtc cgctctaccg aaagttacgg
     7501 gcaccattca acccggtcca gcacggcggc cgggtaaccg acttgctgcc ccgagaatta
     7561 tgcagcattt ttttggtgta tgtgggcccc aaatgaagtg caggtcaaac cttgacagtg
     7621 acgacaaatc gttgggcggg tccagggcga attttgcgac aacatgtcga ggctcagcag
     7681 gacctgcagg catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa
     7741 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     7801 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     7861 ctagagcagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta aactatcagt
     7921 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
     7981 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
     8041 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
     8101 cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt
     8161 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
     8221 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
     8281 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
     8341 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
     8401 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
     8461 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
     8521 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
     8581 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
     8641 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg
     8701 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
     8761 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
     8821 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
     8881 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
     8941 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
     9001 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg
     9061 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
     9121 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
     9181 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
     9241 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
     9301 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
     9361 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
     9421 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
     9481 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
     9541 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
     9601 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
     9661 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
     9721 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
     9781 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
     9841 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
     9901 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
     9961 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
    10021 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
    10081 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
    10141 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
    10201 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct
    10261 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
    10321 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
    10381 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
    10441 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc
    10501 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
    10561 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
    10621 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
    10681 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
    10741 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
    10801 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
    10861 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
    10921 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
    10981 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
    11041 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
    11101 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
    11161 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
    11221 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
    11281 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
    11341 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
    11401 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta
    11461 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
    11521 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
    11581 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
    11641 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg
    11701 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc
    11761 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg
    11821 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
    11881 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
    11941 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
    12001 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
    12061 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    12121 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    12181 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    12241 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    12301 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    12361 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    12421 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    12481 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    12541 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    12601 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    12661 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    12721 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    12781 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    12841 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    12901 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    12961 gattttggtc atgcattcta ggtactaaaa caattcatcc agtaaaatat aatattttat
    13021 tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc
    13081 cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct
    13141 gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct
    13201 gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa
    13261 atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac
    13321 atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt
    13381 cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag
    13441 ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc
    13501 ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg
    13561 aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt
    13621 ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag
    13681 cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat
    13741 cagttttttc aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt
    13801 ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc caattcactg
    13861 ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag
    13921 ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc acaggcagca
    13981 acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac
    14041 ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc
    14101 ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg
    14161 attttgtgcc gagctgccgg tcggggagct gttggctggc tgg
//
