LOCUS       sequence-208238-        9058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes for human EphA2 fluorescently labeled with mTurquoise on the
            C-Terminus via a 15 amino acid (GGS)5 flexible linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Singh DR, Ahmed F, King C, Gupta N, Salotto M, Pasquale EB, Hristova
            K
  TITLE     EphA2 Receptor Unliganded Dimers Suppress EphA2 Pro-tumorigenic 
            Signaling.
  JOURNAL   J Biol Chem. 2015 Nov 6;290(45):27271-9. doi: 
            10.1074/jbc.M115.676866. Epub 2015 Sep 11.
  PUBMED    26363067
REFERENCE   2  (bases 1 to 9058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.676866"; journalName: "J Biol Chem"; date: 
            "2015-11-6- 6"; volume: "290"; issue: "45"; pages: "27271-9"
COMMENT     Sequence Label: EphA2-MT
FEATURES             Location/Qualifiers
     source          1..9058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        22..401
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        402..605
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     556..576
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     650..669
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        650..668
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3691..4404
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(3730..3751)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3991..4010)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4338..4359
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4436..4453)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4442..4666
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4712..5140
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4799..4818)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5009..5030
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5149..5169)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5154..5483
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5334..5469
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5396..5415
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5550..6344
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5604..5623)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6214..6233
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6518..6639
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6555..6574)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6609..6628
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6688..6704)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6688..6704)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6701..6723)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6712..6728
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6736..6742,6743..6760,6761..6766))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6781..6802
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6919..6936)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7090..7678)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7170..7189)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7849..8640,8641..8709))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8472..8491
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8710..8814)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8889..8908)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
       61 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      121 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      181 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      241 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      301 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      361 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      421 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      481 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      541 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      601 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      661 actataggga gacccaagct ggctagcaag cttaccagca acatggagct ccaggcagcc
      721 cgcgcctgct tcgccctgct gtggggctgt gcgctggccg cggccgcggc ggcgcagggc
      781 aaggaagtgg tactgctgga ctttgctgca gctggagggg agctcggctg gctcacacac
      841 ccgtatggca aagggtggga cctgatgcag aacatcatga atgacatgcc gatctacatg
      901 tactccgtgt gcaacgtgat gtctggcgac caggacaact ggctccgcac caactgggtg
      961 taccgaggag aggctgagcg tatcttcatt gagctcaagt ttactgtacg tgactgcaac
     1021 agcttccctg gtggcgccag ctcctgcaag gagactttca acctctacta tgccgagtcg
     1081 gacctggact acggcaccaa cttccagaag cgcctgttca ccaagattga caccattgcg
     1141 cccgatgaga tcaccgtcag cagcgacttc gaggcacgcc acgtgaagct gaacgtggag
     1201 gagcgctccg tggggccgct cacccgcaaa ggcttctacc tggccttcca ggatatcggt
     1261 gcctgtgtgg cgctgctctc cgtccgtgtc tactacaaga agtgccccga gctgctgcag
     1321 ggcctggccc acttccctga gaccatcgcc ggctctgatg caccttccct ggccactgtg
     1381 gccggcacct gtgtggacca tgccgtggtg ccaccggggg gtgaagagcc ccgtatgcac
     1441 tgtgcagtgg atggcgagtg gctggtgccc attgggcagt gcctgtgcca ggcaggctac
     1501 gagaaggtgg aggatgcctg ccaggcctgc tcgcctggat tttttaagtt tgaggcatct
     1561 gagagcccct gcttggagtg ccctgagcac acgctgccat cccctgaggg tgccacctcc
     1621 tgcgagtgtg aggaaggctt cttccgggca cctcaggacc cagcgtcgat gccttgcaca
     1681 cgacccccct ccgccccaca ctacctcaca gccgtgggca tgggtgccaa ggtggagctg
     1741 cgctggacgc cccctcagga cagcgggggc cgcgaggaca ttgtctacag cgtcacctgc
     1801 gaacagtgct ggcccgagtc tggggaatgc gggccgtgtg aggccagtgt gcgctactcg
     1861 gagcctcctc acggactgac ccgcaccagt gtgacagtga gcgacctgga gccccacatg
     1921 aactacacct tcaccgtgga ggcccgcaat ggcgtctcag gcctggtaac cagccgcagc
     1981 ttccgtactg ccagtgtcag catcaaccag acagagcccc ccaaggtgag gctggagggc
     2041 cgcagcacca cctcgcttag cgtctcctgg agcatccccc cgccgcagca gagccgagtg
     2101 tggaagtacg aggtcactta ccgcaagaag ggagactcca acagctacaa tgtgcgccgc
     2161 accgagggtt tctccgtgac cctggacgac ctggccccag acaccaccta cctggtccag
     2221 gtgcaggcac tgacgcagga gggccagggg gccggcagca aggtgcacga attccagacg
     2281 ctgtccccgg agggatctgg caacttggcg gtgattggcg gcgtggctgt cggtgtggtc
     2341 ctgcttctgg tgctggcagg agttggcttc tttatccacc gcaggaggaa gaaccagcgt
     2401 gcccgccagt ccccggagga cgtttacttc tccaagtcag aacaactgaa gcccctgaag
     2461 acatacgtgg acccccacac atatgaggac cccaaccagg ctgtgttgaa gttcactacc
     2521 gagatccatc catcctgtgt cactcggcag aaggtgatcg gagcaggaga gtttggggag
     2581 gtgtacaagg gcatgctgaa gacatcctcg gggaagaagg aggtgccggt ggccatcaag
     2641 acgctgaaag ccggctacac agagaagcag cgagtggact tcctcggcga ggccggcatc
     2701 atgggccagt tcagccacca caacatcatc cgcctagagg gcgtcatctc caaatacaag
     2761 cccatgatga tcatcactga gtacatggag aatggggccc tggacaagtt ccttcgggag
     2821 aaggatggcg agttcagcgt gctgcagctg gtgggcatgc tgcggggcat cgcagctggc
     2881 atgaagtacc tggccaacat gaactatgtg caccgtgacc tggctgcccg caacatcctc
     2941 gtcaacagca acctggtctg caaggtgtct gactttggcc tgtcccgcgt gctggaggac
     3001 gaccccgagg ccacctacac caccagtggc ggcaagatcc ccatccgctg gaccgccccg
     3061 gaggccattt cctaccggaa gttcacctct gccagcgacg tgtggagctt tggcattgtc
     3121 atgtgggagg tgatgaccta tggcgagcgg ccctactggg agttgtccaa ccacgaggtg
     3181 atgaaagcca tcaatgatgg cttccggctc cccacaccca tggactgccc ctccgccatc
     3241 taccagctca tgatgcagtg ctggcagcag gagcgtgccc gccgccccaa gttcgctgac
     3301 atcgtcagca tcctggacaa gctcattcgt gcccctgact ccctcaagac cctggctgac
     3361 tttgaccccc gcgtgtctat ccggctcccc agcacgagcg gctcggaggg ggtgcccttc
     3421 cgcacggtgt ccgagtggct ggagtccatc aagatgcagc agtatacgga gcacttcatg
     3481 gcggccggct acactgccat cgagaaggtg gtgcagatga ccaacgacga catcaagagg
     3541 attggggtgc ggctgcccgg ccaccagaag cgcatcgcct acagcctgct gggactcaag
     3601 gaccaggtga acactgtggg gatccccatc aaaggtaccg gaggaagtgg cggaagtggc
     3661 ggaagtggcg gaagtggagg aagtaccggt agcaagggcg aggagctgtt caccggggtg
     3721 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     3781 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     3841 aagctgcccg tgccctggcc caccctcgtg accaccctgt cctggggcgt gcagtgcttc
     3901 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     3961 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     4021 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     4081 gaggacggca acatcctggg gcacaagctg gagtacaact acatcagcga caacgtctat
     4141 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
     4201 gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     4261 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc
     4321 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     4381 ggcatggacg agctgtacaa gtaatctaga gggcccgttt aaacccgctg atcagcctcg
     4441 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     4501 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     4561 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4621 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4681 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     4741 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4801 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4861 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4921 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4981 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5041 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5101 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     5161 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5221 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5281 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     5341 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     5401 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     5461 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     5521 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     5581 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     5641 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     5701 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     5761 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     5821 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     5881 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     5941 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     6001 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     6061 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     6121 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     6181 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     6241 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     6301 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     6361 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     6421 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     6481 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     6541 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6601 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     6661 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6721 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6781 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6841 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6901 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6961 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7021 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7081 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7141 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7201 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7261 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7321 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7381 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7441 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7501 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7561 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7621 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7681 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7741 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7801 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7861 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7921 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7981 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8041 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8101 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8161 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8221 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8281 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8341 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8401 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8461 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8521 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8581 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8641 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8701 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8761 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8821 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     8881 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     8941 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     9001 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatg
//
