LOCUS       sequence-208239-        9058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes for human EphA2 fluorescently labeled with eYFP on the 
            C-Terminus via a 15 amino acid (GGS)5 flexible linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Singh DR, Ahmed F, King C, Gupta N, Salotto M, Pasquale EB, Hristova
            K
  TITLE     EphA2 Receptor Unliganded Dimers Suppress EphA2 Pro-tumorigenic 
            Signaling.
  JOURNAL   J Biol Chem. 2015 Nov 6;290(45):27271-9. doi: 
            10.1074/jbc.M115.676866. Epub 2015 Sep 11.
  PUBMED    26363067
REFERENCE   2  (bases 1 to 9058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.676866"; journalName: "J Biol Chem"; date: 
            "2015-11-6- 6"; volume: "290"; issue: "45"; pages: "27271-9"
COMMENT     Sequence Label: EphA2-YFP
FEATURES             Location/Qualifiers
     source          1..9058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        41..420
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        421..624
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     575..595
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     669..688
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        669..687
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3710..4423
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(3749..3770)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4010..4029)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4357..4378
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4455..4472)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4461..4685
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4731..5159
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4818..4837)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5028..5049
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5168..5188)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5173..5502
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5353..5488
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5415..5434
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5569..6363
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5623..5642)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6233..6252
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6537..6658
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6574..6593)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6628..6647
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6707..6723)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6707..6723)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6720..6742)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6731..6747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6755..6761,6762..6779,6780..6785))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6800..6821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6938..6955)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7109..7697)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7189..7208)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7868..8659,8660..8728))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8491..8510
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8729..8833)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8908..8927)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt
       61 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      121 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      181 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      241 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      301 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      361 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      421 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
      481 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      541 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      601 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      661 atcgaaatta atacgactca ctatagggag acccaagctg gctagcaagc ttaccagcaa
      721 catggagctc caggcagccc gcgcctgctt cgccctgctg tggggctgtg cgctggccgc
      781 ggccgcggcg gcgcagggca aggaagtggt actgctggac tttgctgcag ctggagggga
      841 gctcggctgg ctcacacacc cgtatggcaa agggtgggac ctgatgcaga acatcatgaa
      901 tgacatgccg atctacatgt actccgtgtg caacgtgatg tctggcgacc aggacaactg
      961 gctccgcacc aactgggtgt accgaggaga ggctgagcgt atcttcattg agctcaagtt
     1021 tactgtacgt gactgcaaca gcttccctgg tggcgccagc tcctgcaagg agactttcaa
     1081 cctctactat gccgagtcgg acctggacta cggcaccaac ttccagaagc gcctgttcac
     1141 caagattgac accattgcgc ccgatgagat caccgtcagc agcgacttcg aggcacgcca
     1201 cgtgaagctg aacgtggagg agcgctccgt ggggccgctc acccgcaaag gcttctacct
     1261 ggccttccag gatatcggtg cctgtgtggc gctgctctcc gtccgtgtct actacaagaa
     1321 gtgccccgag ctgctgcagg gcctggccca cttccctgag accatcgccg gctctgatgc
     1381 accttccctg gccactgtgg ccggcacctg tgtggaccat gccgtggtgc caccgggggg
     1441 tgaagagccc cgtatgcact gtgcagtgga tggcgagtgg ctggtgccca ttgggcagtg
     1501 cctgtgccag gcaggctacg agaaggtgga ggatgcctgc caggcctgct cgcctggatt
     1561 ttttaagttt gaggcatctg agagcccctg cttggagtgc cctgagcaca cgctgccatc
     1621 ccctgagggt gccacctcct gcgagtgtga ggaaggcttc ttccgggcac ctcaggaccc
     1681 agcgtcgatg ccttgcacac gacccccctc cgccccacac tacctcacag ccgtgggcat
     1741 gggtgccaag gtggagctgc gctggacgcc ccctcaggac agcgggggcc gcgaggacat
     1801 tgtctacagc gtcacctgcg aacagtgctg gcccgagtct ggggaatgcg ggccgtgtga
     1861 ggccagtgtg cgctactcgg agcctcctca cggactgacc cgcaccagtg tgacagtgag
     1921 cgacctggag ccccacatga actacacctt caccgtggag gcccgcaatg gcgtctcagg
     1981 cctggtaacc agccgcagct tccgtactgc cagtgtcagc atcaaccaga cagagccccc
     2041 caaggtgagg ctggagggcc gcagcaccac ctcgcttagc gtctcctgga gcatcccccc
     2101 gccgcagcag agccgagtgt ggaagtacga ggtcacttac cgcaagaagg gagactccaa
     2161 cagctacaat gtgcgccgca ccgagggttt ctccgtgacc ctggacgacc tggccccaga
     2221 caccacctac ctggtccagg tgcaggcact gacgcaggag ggccaggggg ccggcagcaa
     2281 ggtgcacgaa ttccagacgc tgtccccgga gggatctggc aacttggcgg tgattggcgg
     2341 cgtggctgtc ggtgtggtcc tgcttctggt gctggcagga gttggcttct ttatccaccg
     2401 caggaggaag aaccagcgtg cccgccagtc cccggaggac gtttacttct ccaagtcaga
     2461 acaactgaag cccctgaaga catacgtgga cccccacaca tatgaggacc ccaaccaggc
     2521 tgtgttgaag ttcactaccg agatccatcc atcctgtgtc actcggcaga aggtgatcgg
     2581 agcaggagag tttggggagg tgtacaaggg catgctgaag acatcctcgg ggaagaagga
     2641 ggtgccggtg gccatcaaga cgctgaaagc cggctacaca gagaagcagc gagtggactt
     2701 cctcggcgag gccggcatca tgggccagtt cagccaccac aacatcatcc gcctagaggg
     2761 cgtcatctcc aaatacaagc ccatgatgat catcactgag tacatggaga atggggccct
     2821 ggacaagttc cttcgggaga aggatggcga gttcagcgtg ctgcagctgg tgggcatgct
     2881 gcggggcatc gcagctggca tgaagtacct ggccaacatg aactatgtgc accgtgacct
     2941 ggctgcccgc aacatcctcg tcaacagcaa cctggtctgc aaggtgtctg actttggcct
     3001 gtcccgcgtg ctggaggacg accccgaggc cacctacacc accagtggcg gcaagatccc
     3061 catccgctgg accgccccgg aggccatttc ctaccggaag ttcacctctg ccagcgacgt
     3121 gtggagcttt ggcattgtca tgtgggaggt gatgacctat ggcgagcggc cctactggga
     3181 gttgtccaac cacgaggtga tgaaagccat caatgatggc ttccggctcc ccacacccat
     3241 ggactgcccc tccgccatct accagctcat gatgcagtgc tggcagcagg agcgtgcccg
     3301 ccgccccaag ttcgctgaca tcgtcagcat cctggacaag ctcattcgtg cccctgactc
     3361 cctcaagacc ctggctgact ttgacccccg cgtgtctatc cggctcccca gcacgagcgg
     3421 ctcggagggg gtgcccttcc gcacggtgtc cgagtggctg gagtccatca agatgcagca
     3481 gtatacggag cacttcatgg cggccggcta cactgccatc gagaaggtgg tgcagatgac
     3541 caacgacgac atcaagagga ttggggtgcg gctgcccggc caccagaagc gcatcgccta
     3601 cagcctgctg ggactcaagg accaggtgaa cactgtgggg atccccatca aaggtaccgg
     3661 aggaagtggc ggaagtggcg gaagtggcgg aagtggagga agtaccggta gcaagggcga
     3721 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     3781 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     3841 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg
     3901 ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     3961 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     4021 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     4081 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     4141 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     4201 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     4261 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
     4321 caaactgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     4381 cgccgccggg atcactctcg gcatggacga gctgtacaag taatctagag ggcccgttta
     4441 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     4501 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     4561 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4621 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     4681 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     4741 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4801 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4861 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4921 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     4981 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     5041 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     5101 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     5161 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     5221 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     5281 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     5341 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5401 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     5461 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     5521 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     5581 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     5641 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     5701 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     5761 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     5821 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     5881 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     5941 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     6001 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     6061 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     6121 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     6181 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     6241 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     6301 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     6361 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     6421 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     6481 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     6541 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6601 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6661 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     6721 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6781 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6841 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6901 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6961 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7021 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7081 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7141 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7201 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7261 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7321 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7381 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7441 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7501 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7561 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7621 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7681 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7741 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7801 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7861 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7921 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7981 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8041 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8101 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8161 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8221 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8281 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8341 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8401 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8461 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8521 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8581 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8641 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8701 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8761 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8821 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     8881 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     8941 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     9001 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgt
//
