LOCUS       sequence-208246-       11880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A bicistronic lentiviral mammalian expression vector for 
            HA-KRasG12V(4B) and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11880)
  AUTHORS   Zhang K, Myllymaki SM, Gao P, Devarajan R, Kytola V, Nykter M, Wei 
            GH, Manninen A
  TITLE     Oncogenic K-Ras upregulates ITGA6 expression via FOSL1 to induce 
            anoikis resistance and synergizes with alphaV-Class integrins to 
            promote EMT.
  JOURNAL   Oncogene. 2017 Oct 12;36(41):5681-5694. doi: 10.1038/onc.2017.177. 
            Epub 2017 Jun 12.
  PUBMED    28604746
REFERENCE   2  (bases 1 to 11880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/onc.2017.177"; journalName: "Oncogene"; date: "2017-10-12- 
            12"; volume: "36"; issue: "41"; pages: "5681-5694"
COMMENT     Sequence Label: pLVET-HA-K-RasG12V-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..11880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2145..3323
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2376..3314
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3271..3291
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3371..3488
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             3524..3550
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(3554..3583,3584..3586,3587..4117)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase K-Ras"
                     /label=K-Ras (G12V)
                     /translation="TEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVI
                     DGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKD
                     SEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIR
                     KHKEKMSKDGKKKKKKSKTKCVIM"
     misc_feature    4144..4710
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4160..4712
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4304..4321)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4531..4550
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4724..4726,4727..4729,4730..5443)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4769..4790)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5030..5049)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5377..5398
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5461..6049
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5514..5534)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5932..5943)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    join(6187..6205,6206..6228,6229..6247,6248..6270,6271..6289,
                     6290..6312,6313..6331,6332..6354,6355..6373,6374..6396,
                     6397..6415,6416..6438,6439..6457)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     LTR             6496..6676
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6701..6719)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6702..6719)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7009..7028)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7242..7261)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7361..7383
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7421..7439)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7507..7611
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7612..7680,7681..8472)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7830..7849)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8643..9231
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9132..9151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9385..9402
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9472..9492)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9477..9806
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9657..9792
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10940..11005
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11135..11155
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11580..11714
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11617..11636)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11671..11690
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2041 accatcgatg tcgacgataa gctttgcaaa gatggataaa gttttaaaca gagaggaatc
     2101 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
     2161 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2221 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     2281 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     2341 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     2401 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     2461 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     2521 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     2581 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     2641 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     2701 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     2761 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     2821 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     2881 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     2941 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     3001 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     3061 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
     3121 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     3181 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     3241 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     3301 ttcttccatt tcaggtgtcg tgaggaattt cgacatttaa atttaattaa tctcgacggt
     3361 atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     3421 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     3481 aaaattttat cgatcacgag actagcctcg aggtttaaac atgtacccat acgatgttcc
     3541 agattacgct gatactgaat ataaacttgt ggtagttgga gctgttggcg taggcaagag
     3601 tgccttgacg atacagctaa ttcagaatca ttttgtggac gaatatgatc caacaataga
     3661 ggattcctac aggaagcaag tagtaattga tggagaaacc tgtctcttgg atattctcga
     3721 cacagcaggt caagaggagt acagtgcaat gagggaccag tacatgagga ctggggaggg
     3781 ctttctttgt gtatttgcca taaataatac taaatcattt gaagatattc accattatag
     3841 agaacaaatt aaaagagtta aggactctga agatgtacct atggtcctag taggaaataa
     3901 atgtgatttg ccttctagaa cagtagacac aaaacaggct caggacttag caagaagtta
     3961 tggaattcct tttattgaaa catcagcaaa gacaagacag ggtgttgatg atgccttcta
     4021 tacattagtt cgagaaattc gaaaacataa agaaaagatg agcaaagacg gtaaaaagaa
     4081 gaaaaagaag tcaaagacaa agtgtgtaat tatgtaagga tccaggccta agcttacgcg
     4141 aattcccccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     4201 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     4261 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     4321 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     4381 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     4441 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     4501 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     4561 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     4621 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
     4681 gtggttttcc tttgaaaaac acgatgataa tatggccaca accatggtga gcaagggtga
     4741 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     4801 caagttcagc gtgtctggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     4861 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     4921 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     4981 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     5041 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     5101 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     5161 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggcgaactt
     5221 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     5281 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     5341 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     5401 cgccgccggg atcactctcg gcatggacga gctgtacaag taagggacta gtcatatgat
     5461 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     5521 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     5581 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     5641 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     5701 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     5761 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     5821 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     5881 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     5941 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     6001 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcggtacgta
     6061 tggccaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     6121 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctcgagtt
     6181 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     6241 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     6301 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     6361 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     6421 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg gatcgatctg
     6481 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6541 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6601 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     6661 tggaaaatct ctagcagcat ctagaattaa ttccgtgtat tctatagtgt cacctaaatc
     6721 gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt
     6781 acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct ctctcgtaaa
     6841 ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac gccgtcccgc
     6901 acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc ctctacgccg
     6961 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
     7021 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
     7081 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
     7141 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
     7201 aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc
     7261 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     7321 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     7381 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     7441 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     7501 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     7561 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     7621 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     7681 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     7741 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     7801 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     7861 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     7921 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     7981 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     8041 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     8101 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     8161 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     8221 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     8281 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     8341 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     8401 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     8461 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     8521 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     8581 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     8641 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     8701 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     8761 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     8821 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     8881 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     8941 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     9001 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     9061 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     9121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     9181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     9241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     9301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     9361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     9421 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt
     9481 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     9541 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     9601 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     9661 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     9721 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     9781 tttttggagg cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt
     9841 ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca
     9901 tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc
     9961 tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac
    10021 atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct
    10081 tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac
    10141 tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg
    10201 acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg
    10261 ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa
    10321 aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg
    10381 ttgttgatat cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg
    10441 tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt
    10501 ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca
    10561 caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg
    10621 ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa
    10681 accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac
    10741 ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac
    10801 gatgatttat acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc
    10861 ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
    10921 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
    10981 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
    11041 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
    11101 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
    11161 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
    11221 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
    11281 aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat
    11341 actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa
    11401 attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag
    11461 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
    11521 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
    11581 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    11641 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    11701 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
    11761 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
    11821 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
//
