LOCUS       sequence-208249-        5912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses an Str-KDEL Hook for the RUSH system and separately 
            Sialyltransferase-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   McCaughey J, Stevenson NL, Cross S, Stephens DJ
  TITLE     ER-to-Golgi trafficking of procollagen in the absence of large 
            carriers.
  JOURNAL   J Cell Biol. 2018 Dec 26. pii: jcb.201806035. doi: 
            10.1083/jcb.201806035.
  PUBMED    30587510
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2018 Dec 26. pii: jcb.201806035. doi: 10.1083/jcb.201806035."
COMMENT     Sequence Label: pStr-KDEL-IRES-ST-mCherry
FEATURES             Location/Qualifiers
     source          1..5912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             169..876
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(314..332)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     582..601
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    1030..1151
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1067..1086)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1253..1269)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1253..1269)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1266..1288)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1277..1293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1301..1307,1308..1325,1326..1331))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1346..1367
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1484..1501)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1655..2243)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1735..1754)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2414..3205,3206..3274))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3037..3056
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3275..3379)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3454..3473)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3645..4024
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4025..4228
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4179..4199
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4273..4292
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4273..4291
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          5056..5285
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5268..5287
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     complement(5510..5527)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5737..5756
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 ataagcttgc cacaacccgg gaggcgcgcc atgattcaca ccaacctgaa gaaaaagttc
       61 agctgctgcg tcctggtctt tcttctgttt gcagtcatct gtgtgtggaa ggaaaagaag
      121 aaagggagtt actatgattc ctttaaattg caaaccaagc ctgcaggtat ggtgagcaag
      181 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
      241 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      301 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
      361 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
      421 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      481 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tacaggacgg cgagttcatc
      541 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
      601 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
      661 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
      721 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
      781 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
      841 cgccactcca ccggcggcat ggacgagctg tacaagggcc ggccttaagg cctcgagggc
      901 caattaatta actctagata actgatcata atcagccata ccacatttgt agaggtttta
      961 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     1021 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     1081 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     1141 aatgtatctt aacgcgtcga gtgcattcta gttgtggttt gtccaaactc atcaatgtat
     1201 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     1261 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     1321 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     1381 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     1441 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     1501 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1561 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1621 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1681 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1741 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1801 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
     1861 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1921 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1981 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2041 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     2101 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2161 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2221 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2281 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     2341 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     2401 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     2461 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     2521 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     2581 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     2641 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     2701 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     2761 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     2821 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     2881 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     2941 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     3001 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     3061 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     3121 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     3181 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     3241 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     3301 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     3361 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     3421 gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg ccgcatagtt
     3481 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     3541 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     3601 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
     3661 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     3721 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     3781 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     3841 atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     3901 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     3961 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     4021 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     4081 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     4141 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     4201 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
     4261 gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc gagctcggat
     4321 cgatatctgc ggcctagcta gcgcttaagg cctgttaacc ggtatggatg tatgcgtccg
     4381 tcttgccctg tggctcctct ggggactcct cctgcaccag ggccagagcc tcagccatag
     4441 tcacagtgag aaggcgacag gaaccagctc gggcgccggt ttaaacgacc ctagcaaaga
     4501 ctcaaaagct caggtgtccg ctgccgaggc tggcattact ggaacatggt acaatcagct
     4561 cgggagcacc tttattgtga ctgctggagc cgatggagcc ctcaccggaa catacgaatc
     4621 tgctgtggga aacgccgaat cacggtacgt cctcactggc cgatacgata gtgcccctgc
     4681 caccgacgga tctgggactg ccctgggatg gactgtcgct tggaaaaaca actaccggaa
     4741 tgctcattct gccacaacat ggagtggaca gtacgtggga ggcgctgagg ctagaatcaa
     4801 tacacagtgg ctgctcacat ctggcacaac cgaggcaaat gcttggaaat ccaccctggt
     4861 gggacatgac acattcacca aagtgaaacc ctccgccgct tcaatcgatg ccgccaaaaa
     4921 agccggagtc aacaacggca atcctctgga tgccgtccag cagctcggac cgaaagatga
     4981 actgtgagcg gccgcataga taactgatcc agtgtgctgg aattaattcg ctgtctgcga
     5041 gggccagctg ttggggtgag tactccctct caaaagcggg catgacttct gcgctaagat
     5101 tgtcagtttc caaaaacgag gaggatttga tattcacctg gcccgcggtg atgcctttga
     5161 gggtggccgc gtccatctgg tcagaaaaga caatcttttt gttgtcaagc ttgaggtgtg
     5221 gcaggcttga gatctggcca tacacttgag tgacaatgac atccactttg cctttctctc
     5281 cacaggtgtc cactcccagg tccaactgca ggtcgagcat gcatctaggg cggccaattc
     5341 cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     5401 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     5461 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     5521 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     5581 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     5641 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     5701 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     5761 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     5821 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     5881 ggggacgtgg ttttcctttg aaaaacacga tg
//
