LOCUS       sequence-208255-        2104 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human USP30 (construct 2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Gersch M, Gladkova C, Schubert AF, Michel MA, Maslen S, Komander D
  TITLE     Mechanism and regulation of the Lys6-selective deubiquitinase USP30.
  JOURNAL   Nat Struct Mol Biol. 2017 Sep 25. doi: 10.1038/nsmb.3475.
  PUBMED    28945249
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2017 Sep 25. doi: 10.1038/nsmb.3475."
COMMENT     Sequence Label: Human USP30 (64-502, construct 2, MG-26-18)
FEATURES             Location/Qualifiers
     source          1..2104
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..45
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             46..699
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     657..679
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             718..741
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2082..2099
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 catcatcatc acagcagcgg tctggaagtt ctgtttcagg gcccgatgtc ccctatacta
       61 ggttattgga aaattaaggg ccttgtgcaa cccactcgac ttcttttgga atatcttgaa
      121 gaaaaatatg aagagcattt gtatgagcgc gatgaaggtg ataaatggcg aaacaaaaag
      181 tttgaattgg gtttggagtt tcccaatctt ccttattata ttgatggtga tgttaaatta
      241 acacagtcta tggccatcat acgttatata gctgacaagc acaacatgtt gggtggttgt
      301 ccaaaagagc gtgcagagat ttcaatgctt gaaggagcgg ttttggatat tagatacggt
      361 gtttcgagaa ttgcatatag taaagacttt gaaactctca aagttgattt tcttagcaag
      421 ctacctgaaa tgctgaaaat gttcgaagat cgtttatgtc ataaaacata tttaaatggt
      481 gatcatgtaa cccatcctga cttcatgttg tatgacgctc ttgatgttgt tttatacatg
      541 gacccaatgt gcctggatgc gttcccaaaa ttagtttgtt ttaaaaaacg tattgaagct
      601 atcccacaaa ttgataagta cttgaaatcc agcaagtata tagcatggcc tttgcagggc
      661 tggcaagcca cgtttggtgg tggcgaccat cctccaaaat cggatctgag cagcggtctg
      721 gaagttctgt ttcagggccc gaaaggtctg gttccgggtc tggttaatct gggtaatacc
      781 tgttttatga atagcctgct gcagggtctg agcgcatgtc cggcatttat tcgttggctg
      841 gaagaattca ccagccagta tagccgtgat cagaaagaac cgcctagcca tcagtatctg
      901 agcctgaccc tgctgcatct gctgaaagca ctgagctgtc aagaggttac cgatgatgaa
      961 gttctggatg caagctgtct gctggatgtt ctgcgtatgt atcgttggca gattagcagc
     1021 tttgaagaac aggatgcaca tgaactgttt catgttatta ccagcagcct ggaagatgaa
     1081 cgtgatcgtc agcctcgtgt tacccacctg tttgatgttc atagcctgga acagcagagc
     1141 gaaattaccc cgaaacaaat tacctgtcgt acccgtggta gtccgcatcc gaccagcaat
     1201 cattggaaaa gccagcatcc gtttcatggt cgtctgacca gtaatatggt ttgtaaacat
     1261 tgcgaacatc agagtccggt tcgttttgat acctttgata gcctgagcct gtcaattccg
     1321 gcagcaacct ggggtcatcc gctgaccctg gatcattgtc tgcatcattt cattagcagc
     1381 gaaagcgttc gtgatgttgt ttgtgataac tgcaccaaaa ttgaagcaaa aggcaccctg
     1441 aatggtgaaa aagttgaaca tcagcgtacc acctttgtga aacagctgaa actgggtaaa
     1501 ctgccgcagt gtctgtgtat tcatctgcag cgtctgtctt ggagcagcca tggtacaccg
     1561 ctgaaacgtc atgaacatgt gcagtttaat gagttcctga tgatggacat ctataaatac
     1621 catctgctgg gccataaacc gagccagcat aatccgaaac tgaacaaaaa tccgggtccg
     1681 accctggaac tgcaggatgg tccgggtgca ccgacaccgg ttctgaatca gcctggcgca
     1741 ccgaaaaccc agatttttat gaacggtgca tgtagcccga gtctgctgcc gaccctgagt
     1801 gcaccgatgc cgtttccgct gccggttgtt ccggattata gcagcagtac ctacctgttt
     1861 cgtctgatgg cagttgttgt tcatcatggt gatatgcata gcggtcattt tgttacctat
     1921 cgtcgtagcc ctccgagcgc acgtaatccg ctgagcacca gtaatcagtg gctgtgggtt
     1981 agtgatgata ccgttcgtaa agcaagcctg caagaagttc tgagcagcag cgcatatctg
     2041 ctgttttacg agcgtgttta aagctttcta gaccatttaa acaccaccac caccaccact
     2101 gaga
//
