LOCUS       sequence-208256-        1917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human USP30 (construct 8).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Gersch M, Gladkova C, Schubert AF, Michel MA, Maslen S, Komander D
  TITLE     Mechanism and regulation of the Lys6-selective deubiquitinase USP30.
  JOURNAL   Nat Struct Mol Biol. 2017 Sep 25. doi: 10.1038/nsmb.3475.
  PUBMED    28945249
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2017 Sep 25. doi: 10.1038/nsmb.3475."
COMMENT     Sequence Label: Human USP30 (64-502, construct 8, MG-26-21)
FEATURES             Location/Qualifiers
     source          1..1917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..63
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             64..717
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     675..697
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             736..759
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             1887..1904
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggtc tggaagttct gtttcagggc
       61 ccgatgtccc ctatactagg ttattggaaa attaagggcc ttgtgcaacc cactcgactt
      121 cttttggaat atcttgaaga aaaatatgaa gagcatttgt atgagcgcga tgaaggtgat
      181 aaatggcgaa acaaaaagtt tgaattgggt ttggagtttc ccaatcttcc ttattatatt
      241 gatggtgatg ttaaattaac acagtctatg gccatcatac gttatatagc tgacaagcac
      301 aacatgttgg gtggttgtcc aaaagagcgt gcagagattt caatgcttga aggagcggtt
      361 ttggatatta gatacggtgt ttcgagaatt gcatatagta aagactttga aactctcaaa
      421 gttgattttc ttagcaagct acctgaaatg ctgaaaatgt tcgaagatcg tttatgtcat
      481 aaaacatatt taaatggtga tcatgtaacc catcctgact tcatgttgta tgacgctctt
      541 gatgttgttt tatacatgga cccaatgtgc ctggatgcgt tcccaaaatt agtttgtttt
      601 aaaaaacgta ttgaagctat cccacaaatt gataagtact tgaaatccag caagtatata
      661 gcatggcctt tgcagggctg gcaagccacg tttggtggtg gcgaccatcc tccaaaatcg
      721 gatctgagca gcggtctgga agttctgttt cagggcccga aaggtctggt tccgggtctg
      781 gttaatctgg gtaatacctg ttttatgaat agcctgctgc agggtctgag cgcatgtccg
      841 gcatttattc gttggctgga agaattcacc agccagtata gccgtgatca gaaagaaccg
      901 cctagccatc agtatctgag cctgaccctg ctgcatctgc tgaaagcact gagctgtcaa
      961 gaggttaccg atgatgaagt tctggatgca agctgtctgc tggatgttct gcgtatgtat
     1021 cgttggcaga ttagcagctt tgaagaacag gatgcacatg aactgtttca tgttattacc
     1081 agcagcctgg aagatgaacg tgatcgtcag cctcgtgtta cccacctgtt tgatgttcat
     1141 agcctggaac agcagagcga aattaccccg aaacaaatta cctgtcgtac ccgtggtagt
     1201 ccgcatccga ccagcaatca ttggaaaagc cagcatccgt ttcatggtcg tctgaccagt
     1261 aatatggttt gtaaacattg cgaacatcag agtccggttc gttttgatac ctttgatagc
     1321 ctgagcctgt caattccggc agcaacctgg ggtcatccgc tgaccctgga tcattgtctg
     1381 catcatttca ttagcagcga aagcgttcgt gatgttgttt gtgataactg caccaaaatt
     1441 gaagcaaaag gcaccctgaa tggtgaaaaa gttgaacatc agcgtaccac ctttgtgaaa
     1501 cagctgaaac tgggtaaact gccgcagtgt ctgtgtattc atctgcagcg tctgtcttgg
     1561 agcagccatg gtacaccgct gaaacgtcat gaacatgtgc agtttaatga ggacctggat
     1621 atggacgagt ataaatacca ctccaatgcg agtacctacc tgtttcgtct gatggcagtt
     1681 gttgttcatc atggtgatat gcatagcggt cattttgtta cctatcgtcg tagccctccg
     1741 agcgcacgta atccgctgag caccagtaat cagtggctgt gggttagtga tgataccgtt
     1801 cgtaaagcaa gcctgcaaga agttctgagc agcagcgcat atctgctgtt ttacgagcgt
     1861 gtttaaagct ttctagacca tttaaacacc accaccacca ccactgagat ccggctg
//
