LOCUS       sequence-208257-        1123 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human USP30 (construct 13).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Gersch M, Gladkova C, Schubert AF, Michel MA, Maslen S, Komander D
  TITLE     Mechanism and regulation of the Lys6-selective deubiquitinase USP30.
  JOURNAL   Nat Struct Mol Biol. 2017 Sep 25. doi: 10.1038/nsmb.3475.
  PUBMED    28945249
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2017 Sep 25. doi: 10.1038/nsmb.3475."
COMMENT     Sequence Label: Human USP30 (64-502, construct 13, MG-26-82)
FEATURES             Location/Qualifiers
     source          1..1123
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..70
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             1096..1113
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtttggtggt ggcgaccatc ctccaaaatc ggatctgagc agcggtctgg aagttctgtt
       61 tcagggcccg aaaggtctgg ttccgggtct ggttaatctg ggtaatacct gttttatgaa
      121 tagcctgctg cagggtctga gcgcatgtcc ggcatttatt cgttggctgg aagaattcac
      181 cagccagtat agccgtgatc agaaagaacc gcctagccat cagtatctga gcctgaccct
      241 gctgcatctg ctgaaagcac tgagctgtca agaggttacc gatgatgaag ttctggatgc
      301 aagctgtctg ctggatgttc tgcgtatgta tcgttggcag attagcagct ttgaagaaca
      361 ggatgcacat gaactgtttc atgttattac cagcagcctg gaagatgaac gtgatggttc
      421 cggctctcat tggaaaagcc agcatccgtt tcatggtcgt ctgaccagta atatggtttg
      481 taaacattgc gaacatcaga gtccggttcg ttttgatacc tttgatagcc tgagcctgtc
      541 aattccggca gcaacctggg gtcatccgct gaccctggat cattgtctgc atcatttcat
      601 tagcagcgaa agcgttcgtg atgttgtttg tgataactgc accaaaattg aagcaaaagg
      661 caccctgaat ggtgaaaaag ttgaacatca gcgtaccacc tttgtgaaac agctgaaact
      721 gggtaaactg ccgcagtgtc tgtgtattca tctgcagcgt ctgtcttgga gcagccatgg
      781 tacaccgctg aaacgtcatg aacatgtgca gtttaatgag gacctgagca tggacgagta
      841 taaataccac tccaatgcga gtacctacct gtttcgtctg atggcagttg ttgttcatca
      901 tggtgatatg catagcggtc attttgttac ctatcgtcgt agccctccga gcgcacgtaa
      961 tccgctgagc accagtaatc agtggctgtg ggttagtgat gataccgttc gtaaagcaag
     1021 cctgcaagaa gttctgagca gcagcgcata tctgctgttt tacgagcgtg tttaaagctt
     1081 tctagaccat ttaaacacca ccaccaccac cactgagatc cgg
//
