LOCUS       sequence-208260-        1687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient transfection of human USP30 C77A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Gersch M, Gladkova C, Schubert AF, Michel MA, Maslen S, Komander D
  TITLE     Mechanism and regulation of the Lys6-selective deubiquitinase USP30.
  JOURNAL   Nat Struct Mol Biol. 2017 Sep 25. doi: 10.1038/nsmb.3475.
  PUBMED    28945249
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2017 Sep 25. doi: 10.1038/nsmb.3475."
COMMENT     Sequence Label: Human USP30 (1-517, C77A, MG-38-16)
FEATURES             Location/Qualifiers
     source          1..1687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1658..1675
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tatattatag ttaaataaga attattatca aatcatttgt atattaatta aaatactata
       61 ctgtaaatta cattttattt acaatcaaag gagatatacc atgctgagca gccgtgccga
      121 agcagcaatg accgcagcag atcgtgcaat tcagcgtttt ctgcgtaccg gtgcagcagt
      181 tcgttataaa gttatgaaaa actggggtgt gattggtggt attgcagcag cactggcagc
      241 aggtatttat gttatttggg gtccgattac cgaacgtaaa aaacgtcgta aaggtctggt
      301 tccgggtctg gttaatctgg gtaataccgc ttttatgaat agcctgctgc agggtctgag
      361 cgcatgtccg gcatttattc gttggctgga agaattcacc agccagtata gccgtgatca
      421 gaaagaaccg cctagccatc agtatctgag cctgaccctg ctgcatctgc tgaaagcact
      481 gagctgtcaa gaggttaccg atgatgaagt tctggatgca agctgtctgc tggatgttct
      541 gcgtatgtat cgttggcaga ttagcagctt tgaagaacag gatgcacatg aactgtttca
      601 tgttattacc agcagcctgg aagatgaacg tgatcgtcag cctcgtgtta cccacctgtt
      661 tgatgttcat agcctggaac agcagagcga aattaccccg aaacaaatta cctgtcgtac
      721 ccgtggtagt ccgcatccga ccagcaatca ttggaaaagc cagcatccgt ttcatggtcg
      781 tctgaccagt aatatggttt gtaaacattg cgaacatcag agtccggttc gttttgatac
      841 ctttgatagc ctgagcctgt caattccggc agcaacctgg ggtcatccgc tgaccctgga
      901 tcattgtctg catcatttca ttagcagcga aagcgttcgt gatgttgttt gtgataactg
      961 caccaaaatt gaagcaaaag gcaccctgaa tggtgaaaaa gttgaacatc agcgtaccac
     1021 ctttgtgaaa cagctgaaac tgggtaaact gccgcagtgt ctgtgtattc atctgcagcg
     1081 tctgtcttgg agcagccatg gtacaccgct gaaacgtcat gaacatgtgc agtttaatga
     1141 gttcctgatg atggacatct ataaatacca tctgctgggc cataaaccga gccagcataa
     1201 tccgaaactg aacaaaaatc cgggtccgac cctggaactg caggatggtc cgggtgcacc
     1261 gacaccggtt ctgaatcagc ctggcgcacc gaaaacccag atttttatga acggtgcatg
     1321 tagcccgagt ctgctgccga ccctgagtgc accgatgccg tttccgctgc cggttgttcc
     1381 ggattatagc agcagtacct acctgtttcg tctgatggca gttgttgttc atcatggtga
     1441 tatgcatagc ggtcattttg ttacctatcg tcgtagccct ccgagcgcac gtaatccgct
     1501 gagcaccagt aatcagtggc tgtgggttag tgatgatacc gttcgtaaag caagcctgca
     1561 agaagttctg agcagcagcg catatctgct gttttacgag cgtgttctga gccgtatgca
     1621 gcatcagagc caagaatgta aaagcgaaga ataaaaacat caccatcacc atcactaagt
     1681 gattaac
//
