LOCUS       sequence-208261-        1152 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Lbpro .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Guarne A, Tormo J, Kirchweger R, Pfistermueller D, Fita I, Skern T
  TITLE     Structure of the foot-and-mouth disease virus leader protease: a 
            papain-like fold adapted for self-processing and eIF4G recognition.
  JOURNAL   EMBO J. 1998 Dec 15;17(24):7469-79. doi: 10.1093/emboj/17.24.7469.
  PUBMED    9857201
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/emboj/17.24"; journalName: "EMBO J"; date: "1998-12-15- 
            15"; volume: "17"; issue: "24"; pages: "7469-79"
COMMENT     Sequence Label: Lbpro (29-195)
FEATURES             Location/Qualifiers
     source          1..1152
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(598..616)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      612..659
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(834..839,840..856,857..862))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(889..907)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        975..1079
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tctagaataa ttttgtttaa ctttaagaag gagatatacc atggagttaa cactgtacaa
       61 cggtgagaag aagacctttt actccaggcc caataaccac gacaactgct ggttgaacgc
      121 catcctccag ttgttcaggt acgttgaaga accattcttc gactgggtct acagttcgcc
      181 tgagaacctc acgcttgaag ccatcaagca gttggaggat ctcacaggac ttgaactgca
      241 tgagggtgga ccacctgctc tcgtgatctg gaacatcaag cacttgctcc acaccggcat
      301 cggcaccgcc tcgcgaccca gcgaggtgtg cgtggtggat ggtacggaca tgtgcttggc
      361 tgatttccat gctggcattt tccttaaggg gcaagaacac gctgtgtttg cgtgtgtcac
      421 ctccaacggg tggtacgcga ttgacgatga ggacttctac ccctggacgc cggacccgtc
      481 cgacgttctg gtgtttgtcc cgtacgatca agaaccactc aacggggaat ggaaagccaa
      541 gtgataagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
      601 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
      661 tgaaaggagg aactatatcc ggatatcccg caagaggccc ggcagtaccg gcataaccaa
      721 gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga
      781 tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag
      841 cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac
      901 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
      961 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     1021 atccgctcat ganacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     1081 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     1141 tttttgctca cc
//
