LOCUS       sequence-208262-        1153 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of ISG15 C-term..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Swatek KN, Aumayr M, Pruneda JN, Visser LJ, Berryman S, Kueck AF, 
            Geurink PP, Ovaa H, van Kuppeveld FJM, Tuthill TJ, Skern T, Komander
            D
  TITLE     Irreversible inactivation of ISG15 by a viral leader protease 
            enables alternative infection detection strategies.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):2371-2376. doi: 
            10.1073/pnas.1710617115. Epub 2018 Feb 20.
  PUBMED    29463763
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1710617115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-03-6- 6"; volume: "115"; issue: "10"; pages: "2371-2376"
COMMENT     Sequence Label: ISG15 C-term. Domain intein/chitin binding dom...
FEATURES             Location/Qualifiers
     source          1..1153
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             270..863
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
     CDS             894..1049
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatggacga acctctgagc atcctggtga
       61 ggaataacaa gggccgcagc agcacctacg aggtgcggct gacgcagacc gtggcccacc
      121 tgaagcagca agtgagcggg ctggagggtg tgcaggacga cctgttctgg ctgaccttcg
      181 aggggaagcc cctggaggac cagctcccgc tgggggagta cggcctcaag cccctgagca
      241 ccgtgttcat gaatctgcgc ctgcgggggt gcatcacggg agatgcacta gttgccctac
      301 ccgagggcga gtcggtacgc atcgccgaca tcgtgccggg tgcgcggccc aacagtgaca
      361 acgccatcga cctgaaagtc cttgaccggc atggcaatcc cgtgctcgcc gaccggctgt
      421 tccactccgg cgagcatccg gtgtacacgg tgcgtacggt cgaaggtctg cgtgtgacgg
      481 gcaccgcgaa ccacccgttg ttgtgtttgg tcgacgtcgc cggggtgccg accctgctgt
      541 ggaagctgat cgacgaaatc aagccgggcg attacgcggt gattcaacgc agcgcattca
      601 gcgtcgactg tgcaggtttt gcccgcggga aacccgaatt tgcgcccaca acctacacag
      661 tcggcgtccc tggactggtg cgtttcttgg aagcacacca ccgagacccg gacgcccaag
      721 ctatcgccga cgagctgacc gacgggcggt tctactacgc gaaagtcgcc agtgtcaccg
      781 acgccggcgt gcagccggtg tatagccttc gtgtcgacac ggcagaccac gcgtttatca
      841 cgaacgggtt cgtcagccac gctactggcc tcaccggtct gaactcaggc ctcacgacaa
      901 atcctggtgt atccgcttgg caggtcaaca cagcttatac tgcgggacaa ttggtcacat
      961 ataacggcaa gacgtataaa tgtttgcagc cccacacctc cttggcagga tgggaaccat
     1021 ccaacgttcc tgccttgtgg cagcttcaat gactgcaaga aggggatccg gctgctaaca
     1081 aagcccgaaa ggaagctgaa ttggctgctg ccaccgctga acaataacta gcataacccc
     1141 ttggggcctc taa
//
