LOCUS       sequence-208265-        1146 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of proISG15 C-term. domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Swatek KN, Aumayr M, Pruneda JN, Visser LJ, Berryman S, Kueck AF, 
            Geurink PP, Ovaa H, van Kuppeveld FJM, Tuthill TJ, Skern T, Komander
            D
  TITLE     Irreversible inactivation of ISG15 by a viral leader protease 
            enables alternative infection detection strategies.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):2371-2376. doi: 
            10.1073/pnas.1710617115. Epub 2018 Feb 20.
  PUBMED    29463763
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1710617115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-03-6- 6"; volume: "115"; issue: "10"; pages: "2371-2376"
COMMENT     Sequence Label: proISG15 C-term. domain (79-165)
FEATURES             Location/Qualifiers
     source          1..1146
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..94
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             379..396
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(449..467)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      463..510
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      547..1002
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(634..653)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     844..865
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggt ctggaagttc tgtttcaggg cccggacgaa cctctgagca tcctggtgag
      121 gaataacaag ggccgcagca gcacctacga ggtgcggctg acgcagaccg tggcccacct
      181 gaagcagcaa gtgagcgggc tggagggtgt gcaggacgac ctgttctggc tgaccttcga
      241 ggggaagccc ctggaggacc agctcccgct gggggagtac ggcctcaagc ccctgagcac
      301 cgtgttcatg aatctgcgcc tgcggggagg cggcacagag cctggcgggc ggagctaaag
      361 ctttctagac catttaaaca ccaccaccac caccactgag atccggctgc taacaaagcc
      421 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
      481 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg
      541 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
      601 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      661 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      721 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      781 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      841 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      901 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      961 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     1021 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1081 catgaattaa ttcttagaaa actcatcgag catcaatgaa actgcattta ttctatcagg
     1141 atatca
//
