LOCUS       sequence-208266-        1173 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of proISG15 C-term. domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Swatek KN, Aumayr M, Pruneda JN, Visser LJ, Berryman S, Kueck AF, 
            Geurink PP, Ovaa H, van Kuppeveld FJM, Tuthill TJ, Skern T, Komander
            D
  TITLE     Irreversible inactivation of ISG15 by a viral leader protease 
            enables alternative infection detection strategies.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):2371-2376. doi: 
            10.1073/pnas.1710617115. Epub 2018 Feb 20.
  PUBMED    29463763
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1710617115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-03-6- 6"; volume: "115"; issue: "10"; pages: "2371-2376"
COMMENT     Sequence Label: mature ISG15 C-term. domain (79-157)
FEATURES             Location/Qualifiers
     source          1..1173
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..90
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             351..368
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(421..439)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      435..482
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      519..974
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(606..625)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     816..837
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc
       61 agcggtctgg aagttctgtt tcagggcccg gacgaacctc tgagcatcct ggtgaggaat
      121 aacaagggcc gcagcagcac ctacgaggtg cggctgacgc agaccgtggc ccacctgaag
      181 cagcaagtga gcgggctgga gggtgtgcag gacgacctgt tctggctgac cttcgagggg
      241 aagcccctgg aggaccagct cccgctgggg gagtacggcc tcaagcccct gagcaccgtg
      301 ttcatgaatc tgcgcctgcg gggaggctaa agctttctag accatttaaa caccaccacc
      361 accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
      421 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
      481 tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt
      541 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
      601 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
      661 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
      721 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
      781 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
      841 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
      901 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
      961 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     1021 tttttctaaa tacattcaaa tatgtatccg ctcatgaatt aattcttaga aaaactcatc
     1081 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
     1141 aagccgtttc tgtaatgaag gaaaaactca ccg
//
