LOCUS       sequence-208267-        1203 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mutant proISG15 C-term. domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Swatek KN, Aumayr M, Pruneda JN, Visser LJ, Berryman S, Kueck AF, 
            Geurink PP, Ovaa H, van Kuppeveld FJM, Tuthill TJ, Skern T, Komander
            D
  TITLE     Irreversible inactivation of ISG15 by a viral leader protease 
            enables alternative infection detection strategies.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):2371-2376. doi: 
            10.1073/pnas.1710617115. Epub 2018 Feb 20.
  PUBMED    29463763
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1710617115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-03-6- 6"; volume: "115"; issue: "10"; pages: "2371-2376"
COMMENT     Sequence Label: mature ISG15 C-term. Domain (79-165, W123A)
FEATURES             Location/Qualifiers
     source          1..1203
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..92
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             377..394
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(447..465)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      461..508
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      545..1000
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(632..651)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     842..863
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca
       61 gcagcggtct ggaagttctg tttcagggcc cggacgaacc tctgagcatc ctggtgagga
      121 ataacaaggg ccgcagcagc acctacgagg tgcggctgac gcagaccgtg gcccacctga
      181 agcagcaagt gagcgggctg gagggtgtgc aggacgacct gttcgcgctg accttcgagg
      241 ggaagcccct ggaggaccag ctcccgctgg gggagtacgg cctcaagccc ctgagcaccg
      301 tgttcatgaa tctgcgcctg cggggaggcg gcacagagcc tggcgggcgg agctaaagct
      361 ttctagacca tttaaacacc accaccacca ccactgagat ccggctgcta acaaagcccg
      421 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
      481 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa
      541 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
      601 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
      661 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
      721 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
      781 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
      841 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
      901 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
      961 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     1021 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1081 tgaattaatt cttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     1141 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaaa aactcaccga
     1201 ggc
//
