LOCUS       sequence-208268-        1172 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Met1 diUb.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Swatek KN, Aumayr M, Pruneda JN, Visser LJ, Berryman S, Kueck AF, 
            Geurink PP, Ovaa H, van Kuppeveld FJM, Tuthill TJ, Skern T, Komander
            D
  TITLE     Irreversible inactivation of ISG15 by a viral leader protease 
            enables alternative infection detection strategies.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):2371-2376. doi: 
            10.1073/pnas.1710617115. Epub 2018 Feb 20.
  PUBMED    29463763
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1710617115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-03-6- 6"; volume: "115"; issue: "10"; pages: "2371-2376"
COMMENT     Sequence Label: Met1 diUb (1-152)
FEATURES             Location/Qualifiers
     source          1..1172
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..91
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             92..319
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             92..319
                     /codon_start=1
                     /gene="Homo sapiens UBC"
                     /product="human ubiquitin C"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             320..547
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             574..591
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(644..662)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      658..705
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      742..1168
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(829..848)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1038..1059
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttgtttaact ttaagaagga gatataccat gggcagcagc catcatcatc atcatcacag
       61 cagcggtctg gaagttctgt ttcagggccc gatgcagatc ttcgtgaaga ccctgactgg
      121 taagaccatc actctcgaag tggagccgag tgacaccatt gagaatgtca aggcaaagat
      181 ccaagacaag gaaggcatcc ctcctgacca gcagaggttg atctttgctg ggaagcagct
      241 ggaagatgga cgcaccctgt ctgactacaa catccagaaa gagtccaccc tgcacctggt
      301 cctccgtctc agaggtggga tgcaaatatt tgtcaaaaca ctaacaggaa aaacaataac
      361 actagaggtc gaaccctctg atacaataga aaacgtgaaa gccaaaatac aggataaaga
      421 gggaataccc cccgatcaac aaaggctaat attcgccgga aaacaactag aggacgggag
      481 gacactaagt gattataata tacaaaagga aagtacacta catctagtgc taaggttaag
      541 aggtgggtga taaagctttc tagaccattt aaacaccacc accaccacca ctgagatccg
      601 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
      661 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
      721 atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
      781 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
      841 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
      901 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaac ttgattaggg
      961 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     1021 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     1081 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     1141 gctgatttaa caaaatttaa cgcgaatttt aa
//
