LOCUS       sequence-208276-        7928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To test the effect of sequence on TDP43 splicing activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7928)
  TITLE     Rajat Rohatgi TDP43 plasmids
REFERENCE   2  (bases 1 to 7928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHBS1108 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 ∆RRM]
FEATURES             Location/Qualifiers
     source          1..7928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(212..231)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     481..499
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2121..2130
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        2283..2586
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2587..2790
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2741..2761
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2852..2861
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2858..3559
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     misc_feature    3560..3625
                     /label=MCS
                     /note="multiple cloning site"
     CDS             3650..3706
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(4498..4522)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          4577..5149
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(5281..5300)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5346..5401
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5400..5419)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5799..5816)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5970..6558)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6050..6069)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6729..7520,7521..7589))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7352..7371
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7590..7694)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7762..7780
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atggtgtagt cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt gtaggcgccg
       61 ggcagctgca cgggcttctt ggccttgtag gtggtcttga cctcagcgtc gtagtggccg
      121 ccgtccttca gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac
      181 atccgctcgg aggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcggag
      241 gggaagttgg tgccgcgcag cttcaccttg tagatgaact cgccgtcctg cagggaggag
      301 tcctgggtca cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag
      361 ccctcgggga aggacagctt caagtagtcg gggatgtcgg cggggtgctt cacgtaggcc
      421 ttggagccgt acatgaactg aggggacagg atgtcccagg cgaagggcag ggggccaccc
      481 ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg ggcggccctg aggaaaaaaa
      541 aggaaagggc atgctcgcca tgtgcaagat acagtgttga atgtggtgca aaatataaag
      601 atgtggaaga aaaagccact gaaaataata tgaggaaata aaaaactaga gtaaattatc
      661 agtggaacaa tttctattat acaagacact acacccatac attctcctaa tgctcatgta
      721 agaatttctc caaaaatacc ttccagcact acaaactaga aaaaaaaaga gacatggaca
      781 ccaaattaag ttcttaatag tgaagaacaa aagaactacc ttgcctgctc cagtggatcc
      841 agcaaccgcc aacaactgtc ctctttctat cttgaaatta atatctttca ggacaggagt
      901 accaagaagt gagaaattac tgaagaagag gctgtcatca ccattagaag tttttctatt
      961 gttattgttt tgttttgctt tctcaaatta ttccccaaat ccctgttaaa aaaaaacaca
     1021 cacacacaca cacacacaca catcaaaaat aaaagatgag tttgtcagat attttcaata
     1081 gatgcttgtt ttatgtacca tactcacctc gccctcgccc tcgatctcga actcgtggcc
     1141 gttcacggag ccctccatgt gcaccttgaa gcgcatgaac tccttgatga tggccatgtt
     1201 atcctcctcg cccttgctgg ttccggtgcc tccactttca tcttcacctt tagtgatacc
     1261 ggctgccgtc acgaactcct tcaggaccat gtgatcgcgc ttctcgttcg ggtctttgct
     1321 cagcgtggtc tgggtgctca gatagtggtt gtccggcagg cgcaccgggc cgtcgccgat
     1381 cggggtgttc tgctgtttgt ggtcggcttc ctgctggctg ccgtcctcaa cgttgtggcg
     1441 gatcttgaag ttagccttga tgccgttctt ctgcttgtcg gcttcgatac ggacgttgtg
     1501 gctgttgtgg ttgtactcca gcttgtgacc caggatgttg ccgtcctcct tgaagtcgtt
     1561 gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct ccgcgcgggt
     1621 cttgtaggtg ccgtcgtcct tgaactcgat ggtgcgctcc tggacgtagc cttccggcat
     1681 ggcggacttg aagaagtcgt gacgcttcat gtggtccggg tagcggctga agcactgcac
     1741 gccgtaggtc agggtggtca cgagggtcgg ccacggcacc ggcagcttgc cggtggtgca
     1801 gatgaacttc agggtcagct tgccctcggt ggcatcgccc tcgccctcgc cacgcacgct
     1861 gaacttgtgg ccgtttacgt cgccgtccag ctcgactttg ataggcacag tacctgtaaa
     1921 tagttcctct cctttactgg taccatcatc agggaatgag cttacatttc tcctcttcag
     1981 catctggtca tccttcttag ctttcctcag ctccacattg acctctattc tgcgacgcct
     2041 catttctgta ctgtcttttc ccttgttctt gaatctgtga agacgggcag ctggtgtatt
     2101 agcattctcg ttggtggatc ccatggtggc accggtgaat tctccaggcg atctgacggt
     2161 tcactaaacg agctctgctt atataggcct cccaccgtac acgccacctc gacatactcg
     2221 agtagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     2281 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     2341 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     2401 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     2461 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     2521 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     2581 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     2641 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     2701 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     2761 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctaggga
     2821 tcctctagtc agctgacgcg ttaccaccgg tgccaccatg ggaagcgagc tgattaagga
     2881 gaacatgcac atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac
     2941 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga
     3001 gggcggccct ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa
     3061 gaccttcatc aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg
     3121 cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga
     3181 caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac
     3241 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct
     3301 gtaccccgct gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg
     3361 gagccatctg atcgcaaacg ccaagaccac atatagatcc aagaaacccg ctaagaacct
     3421 caagatgcct ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa
     3481 cgagacctac gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa
     3541 actggggcac aagcttaatt ccggactcag atctcgagct caagcttcga attctgcagt
     3601 cgacggtacc gcgggcccgg gatccaccgg atctagagga ggaagcggag ctactaactt
     3661 cagcctgctg aagcaggctg gagacgtgga ggagaaccct ggacctatgt ctgaatatat
     3721 tcgggtaacc gaagatgaga acgatgagcc cattgaaata ccatcggaag acgatgggac
     3781 ggtgctgctc tccacggtta cagcccagtt tccaggggcg tgtgggcttc gctacaggaa
     3841 tccagtgtct cagtgtatga gaggtgtccg gctggtagaa ggaattctgc atgccccaga
     3901 tgctggctgg ggaaatctgg tgtatgttgt caactatcca aaagataaca aaagaaaaat
     3961 ggatgagaca gatgcttcat cagcagtgaa agtgaaaaga gcagtccaga aaacatcctc
     4021 caatgccgaa cctaagcaca atagcaatag acagttagaa agaagtggaa gatttggtgg
     4081 taatccaggt ggctttggga atcagggtgg atttggtaat agcagagggg gtggagctgg
     4141 tttgggaaac aatcaaggta gtaatatggg tggtgggatg aactttggtg cgttcagcat
     4201 taatccagcc atgatggctg ccgcccaggc agcactacag agcagttggg gtatgatggg
     4261 catgttagcc agccagcaga accagtcagg cccatcgggt aataaccaaa accaaggcaa
     4321 catgcagagg gagccaaacc aggccttcgg ttctggaaat aactcttata gtggctctaa
     4381 ttctggtgca gcaattggtt ggggatcagc atccaatgca gggtcgggca gtggttttaa
     4441 tggaggcttt ggctcaagca tggattctaa gtcttctggc tggggaatgt agactagacc
     4501 cagctttctt gtacaaagtg gttgatcctc tactagtaag cttgcatgcg atatctctag
     4561 agctgagaac ttcagggtga gtttggggac ccttgattgt tctttctttt tcgctattgt
     4621 aaaattcatg ttatatggag ggggcaaagt tttcagggtg ttgtttagaa tgggaagatg
     4681 tcccttgtat caccatggac cctcatgata attttgtttc tttcactttc tactctgttg
     4741 acaaccattg tctcctctta ttttcttttc attttctgta actttttcgt taaactttag
     4801 cttgcatttg taacgaattt ttaaattcac ttttgtttat ttgtcagatt gtaagtactt
     4861 tctctaatca cttttttttc aaggcaatca gggtatatta tattgtactt cagcacagtt
     4921 ttagagaaca attgttataa ttaaatgata aggtagaata tttctgcata taaattctgg
     4981 ctggcgtgga aatattctta ttggtagaaa caactacacc ctggtcatca tcctgccttt
     5041 ctctttatgg ttacaatgat atacactgtt tgagatgagg ataaaatact ctgagtccaa
     5101 accgggcccc tctgctaacc atgttcatgc cttcttctct ttcctacagc tcctgggcaa
     5161 cgtgctggtt gttgtgctgt ctcatcattt tggcaaagaa ttcactcctc aggtgcaggc
     5221 tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga
     5281 tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc
     5341 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     5401 actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta
     5461 gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg
     5521 tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac
     5581 ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa
     5641 attttcctta catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat
     5701 agctgtccct cttctcttat ggagatccct cgactgcatt aatgaatcgg ccaacgcgcg
     5761 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     5821 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     5881 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     5941 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6001 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6061 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6121 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6181 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6241 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6301 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6361 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     6421 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6481 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6541 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6601 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     6661 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     6721 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     6781 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     6841 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     6901 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     6961 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7021 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7081 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7141 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7201 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7261 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7321 ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta
     7381 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7441 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     7501 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     7561 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     7621 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     7681 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     7741 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct tcactcgacg
     7801 cggccagctt ggtcgagctg gatacttccc gtccgccagg gggacatgcc ggcgatgctg
     7861 aaggtcgcgc gcattcccga tgaagaggcc ggttaccgcc tgttgacctg gtgggacggg
     7921 cagggcgc
//
