LOCUS       sequence-208278-        7961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To test the effect of sequence on TDP43 splicing activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7961)
  TITLE     Rajat Rohatgi TDP43 plasmids
REFERENCE   2  (bases 1 to 7961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHBS1109 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43  ∆...
FEATURES             Location/Qualifiers
     source          1..7961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(242..261)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     511..529
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2151..2160
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        2313..2616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2617..2820
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2771..2791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2882..2891
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2888..3589
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     misc_feature    3590..3655
                     /label=MCS
                     /note="multiple cloning site"
     CDS             3680..3736
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(4531..4555)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          4610..5182
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(5314..5333)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5379..5434
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5433..5452)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5832..5849)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6003..6591)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6083..6102)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6762..7553,7554..7622))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7385..7404
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7623..7727)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7795..7813
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cggccctcgg cgcgttcgta ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg
       61 tccaacttga tgttgacgtt gtaggcgccg ggcagctgca cgggcttctt ggccttgtag
      121 gtggtcttga cctcagcgtc gtagtggccg ccgtccttca gcttcagcct ctgcttgatc
      181 tcgcccttca gggcgccgtc ctcggggtac atccgctcgg aggaggcctc ccagcccatg
      241 gtcttcttct gcattacggg gccgtcggag gggaagttgg tgccgcgcag cttcaccttg
      301 tagatgaact cgccgtcctg cagggaggag tcctgggtca cggtcaccac gccgccgtcc
      361 tcgaagttca tcacgcgctc ccacttgaag ccctcgggga aggacagctt caagtagtcg
      421 gggatgtcgg cggggtgctt cacgtaggcc ttggagccgt acatgaactg aggggacagg
      481 atgtcccagg cgaagggcag ggggccaccc ttggtcacct tcagcttggc ggtctgggtg
      541 ccctcgtagg ggcggccctg aggaaaaaaa aggaaagggc atgctcgcca tgtgcaagat
      601 acagtgttga atgtggtgca aaatataaag atgtggaaga aaaagccact gaaaataata
      661 tgaggaaata aaaaactaga gtaaattatc agtggaacaa tttctattat acaagacact
      721 acacccatac attctcctaa tgctcatgta agaatttctc caaaaatacc ttccagcact
      781 acaaactaga aaaaaaaaga gacatggaca ccaaattaag ttcttaatag tgaagaacaa
      841 aagaactacc ttgcctgctc cagtggatcc agcaaccgcc aacaactgtc ctctttctat
      901 cttgaaatta atatctttca ggacaggagt accaagaagt gagaaattac tgaagaagag
      961 gctgtcatca ccattagaag tttttctatt gttattgttt tgttttgctt tctcaaatta
     1021 ttccccaaat ccctgttaaa aaaaaacaca cacacacaca cacacacaca catcaaaaat
     1081 aaaagatgag tttgtcagat attttcaata gatgcttgtt ttatgtacca tactcacctc
     1141 gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa
     1201 gcgcatgaac tccttgatga tggccatgtt atcctcctcg cccttgctgg ttccggtgcc
     1261 tccactttca tcttcacctt tagtgatacc ggctgccgtc acgaactcct tcaggaccat
     1321 gtgatcgcgc ttctcgttcg ggtctttgct cagcgtggtc tgggtgctca gatagtggtt
     1381 gtccggcagg cgcaccgggc cgtcgccgat cggggtgttc tgctgtttgt ggtcggcttc
     1441 ctgctggctg ccgtcctcaa cgttgtggcg gatcttgaag ttagccttga tgccgttctt
     1501 ctgcttgtcg gcttcgatac ggacgttgtg gctgttgtgg ttgtactcca gcttgtgacc
     1561 caggatgttg ccgtcctcct tgaagtcgtt gcccttcagc tcgatgcggt tcaccagggt
     1621 gtcgccctcg aacttcacct ccgcgcgggt cttgtaggtg ccgtcgtcct tgaactcgat
     1681 ggtgcgctcc tggacgtagc cttccggcat ggcggacttg aagaagtcgt gacgcttcat
     1741 gtggtccggg tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtcgg
     1801 ccacggcacc ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccctcggt
     1861 ggcatcgccc tcgccctcgc cacgcacgct gaacttgtgg ccgtttacgt cgccgtccag
     1921 ctcgactttg ataggcacag tacctgtaaa tagttcctct cctttactgg taccatcatc
     1981 agggaatgag cttacatttc tcctcttcag catctggtca tccttcttag ctttcctcag
     2041 ctccacattg acctctattc tgcgacgcct catttctgta ctgtcttttc ccttgttctt
     2101 gaatctgtga agacgggcag ctggtgtatt agcattctcg ttggtggatc ccatggtggc
     2161 accggtgaat tctccaggcg atctgacggt tcactaaacg agctctgctt atataggcct
     2221 cccaccgtac acgccacctc gacatactcg agtagttatt aatagtaatc aattacgggg
     2281 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     2341 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     2401 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     2461 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     2521 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     2581 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     2641 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     2701 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     2761 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     2821 ggtttagtga accgtcagat ccgctaggga tcctctagtc agctgacgcg ttaccaccgg
     2881 tgccaccatg ggaagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg
     2941 caccgtggac aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg
     3001 cacccagacc atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat
     3061 cctggctact agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc
     3121 cgacttcttc aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga
     3181 agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta
     3241 caacgtcaag atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac
     3301 actcggctgg gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag
     3361 aaacgacatg gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg ccaagaccac
     3421 atatagatcc aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta
     3481 cagactggaa agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc
     3541 agtggccaga tactgcgacc tccctagcaa actggggcac aagcttaatt ccggactcag
     3601 atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg gatccaccgg
     3661 atctagagga ggaagcggag ctactaactt cagcctgctg aagcaggctg gagacgtgga
     3721 ggagaaccct ggacctatgt ctgaatatat tcgggtaacc gaagatgaga acgatgagcc
     3781 cattgaaata ccatcggaag acgatgggac ggtgctgctc tccacggtta cagcccagtt
     3841 tccaggggcg tgtgggcttc gctacaggaa tccagtgtct cagtgtatga gaggtgtccg
     3901 gctggtagaa ggaattctgc atgccccaga tgctggctgg ggaaatctgg tgtatgttgt
     3961 caactatcca aaagataaca aaagaaaaat ggatgagaca gatgcttcat cagcagtgaa
     4021 agtgaaaaga gcagtccaga aaacatccga tttaatagtg ttgggtctcc catggaaaac
     4081 aaccgaacag gacctgaaag agtattttag tacctttgga gaagttctta tggtgcaggt
     4141 caagaaagat cttaagactg gtcattcaaa ggggtttggc tttgttcgtt ttacggaata
     4201 tgaaacacaa gtgaaagtaa tgtcacagcg acatatgata gatggacgat ggtgtgactg
     4261 caaacttcct aattctaagc aaagccaaga tgagcctttg agaagcagaa aagtgtttgt
     4321 ggggcgctgt acagaggaca tgactgagga tgagctgcgg gagttcttct ctcagtacgg
     4381 ggatgtgatg gatgtcttca tccccaagcc attcagggcc tttgcctttg ttacatttgc
     4441 agatgatcag attgcgcagt ctctttgtgg agaggacttg atcattaaag gaatcagcgt
     4501 tcatatatcc aatgccgaac cttagactag acccagcttt cttgtacaaa gtggttgatc
     4561 ctctactagt aagcttgcat gcgatatctc tagagctgag aacttcaggg tgagtttggg
     4621 gacccttgat tgttctttct ttttcgctat tgtaaaattc atgttatatg gagggggcaa
     4681 agttttcagg gtgttgttta gaatgggaag atgtcccttg tatcaccatg gaccctcatg
     4741 ataattttgt ttctttcact ttctactctg ttgacaacca ttgtctcctc ttattttctt
     4801 ttcattttct gtaacttttt cgttaaactt tagcttgcat ttgtaacgaa tttttaaatt
     4861 cacttttgtt tatttgtcag attgtaagta ctttctctaa tcactttttt ttcaaggcaa
     4921 tcagggtata ttatattgta cttcagcaca gttttagaga acaattgtta taattaaatg
     4981 ataaggtaga atatttctgc atataaattc tggctggcgt ggaaatattc ttattggtag
     5041 aaacaactac accctggtca tcatcctgcc tttctcttta tggttacaat gatatacact
     5101 gtttgagatg aggataaaat actctgagtc caaaccgggc ccctctgcta accatgttca
     5161 tgccttcttc tctttcctac agctcctggg caacgtgctg gttgttgtgc tgtctcatca
     5221 ttttggcaaa gaattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt
     5281 ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg
     5341 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
     5401 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     5461 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct
     5521 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc
     5581 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg
     5641 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag
     5701 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc
     5761 cctcgactgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     5821 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     5881 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     5941 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6001 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6061 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6121 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6181 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6241 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6301 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6361 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6421 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     6481 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6541 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6601 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6661 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     6721 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     6781 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6841 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6901 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6961 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7021 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7081 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7141 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7201 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7261 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7321 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca
     7381 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     7441 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     7501 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     7561 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     7621 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     7681 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     7741 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     7801 cgtatcacga ggccctttcg tcttcactcg acgcggccag cttggtcgag ctggatactt
     7861 cccgtccgcc agggggacat gccggcgatg ctgaaggtcg cgcgcattcc cgatgaagag
     7921 gccggttacc gcctgttgac ctggtgggac gggcagggcg c
//
