LOCUS       sequence-208282-        6149 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To test the effect of sequence on TDP43 splicing activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6149)
  TITLE     Rajat Rohatgi TDP43 plasmids
REFERENCE   2  (bases 1 to 6149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHBS1138 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43  ∆...
FEATURES             Location/Qualifiers
     source          1..6149
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      186..195
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        348..651
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        652..855
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     806..826
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      917..926
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             923..1624
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     misc_feature    1625..1690
                     /label=MCS
                     /note="multiple cloning site"
     CDS             1715..1771
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(2719..2743)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          2798..3370
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(3502..3521)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3567..3622
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3621..3640)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4020..4037)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4191..4779)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4271..4290)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4950..5741,5742..5810))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5573..5592
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5811..5915)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5983..6001
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 actggtacca tcatcaggga atgagcttac atttctcctc ttcagcatct ggtcatcctt
       61 cttagctttc ctcagctcca cattgacctc tattctgcga cgcctcattt ctgtactgtc
      121 ttttcccttg ttcttgaatc tgtgaagacg ggcagctggt gtattagcat tctcgttggt
      181 ggatcccatg gtggcaccgg tgaattctcc aggcgatctg acggttcact aaacgagctc
      241 tgcttatata ggcctcccac cgtacacgcc acctcgacat actcgagtag ttattaatag
      301 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      361 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      421 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      481 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      541 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      601 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      661 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      721 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      781 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      841 tatataagca gagctggttt agtgaaccgt cagatccgct agggatcctc tagtcagctg
      901 acgcgttacc accggtgcca ccatgggaag cgagctgatt aaggagaaca tgcacatgaa
      961 gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg agggcgaagg
     1021 caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctccc
     1081 cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct tcatcaacca
     1141 cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca catgggagag
     1201 agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga
     1261 cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca acggccctgt
     1321 gatgcagaag aaaacactcg gctgggaggc cttcaccgag acgctgtacc ccgctgacgg
     1381 cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc atctgatcgc
     1441 aaacgccaag accacatata gatccaagaa acccgctaag aacctcaaga tgcctggcgt
     1501 ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaga cctacgtcga
     1561 gcagcacgag gtggcagtgg ccagatactg cgacctccct agcaaactgg ggcacaagct
     1621 taattccgga ctcagatctc gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg
     1681 cccgggatcc accggatcta gaggaggaag cggagctact aacttcagcc tgctgaagca
     1741 ggctggagac gtggaggaga accctggacc tatgacatcc gatttaatag tgttgggtct
     1801 cccatggaaa acaaccgaac aggacctgaa agagtatttt agtacctttg gagaagttct
     1861 tatggtgcag gtcaagaaag atcttaagac tggtcattca aaggggtttg gctttgttcg
     1921 ttttacggaa tatgaaacac aagtgaaagt aatgtcacag cgacatatga tagatggacg
     1981 atggtgtgac tgcaaacttc ctaattctaa gcaaagccaa gatgagcctt tgagaagcag
     2041 aaaagtgttt gtggggcgct gtacagagga catgactgag gatgagctgc gggagttctt
     2101 ctctcagtac ggggatgtga tggatgtctt catccccaag ccattcaggg cctttgcctt
     2161 tgttacattt gcagatgatc agattgcgca gtctctttgt ggagaggact tgatcattaa
     2221 aggaatcagc gttcatatat ccaatgccga acctaagcac aatagcaata gacagttaga
     2281 aagaagtgga agatttggtg gtaatccagg tggctttggg aatcagggtg gatttggtaa
     2341 tagcagaggg ggtggagctg gtttgggaaa caatcaaggt agtaatatgg gtggtgggat
     2401 gaactttggt gcgttcagca ttaatccagc catgatggct gccgcccagg cagcactaca
     2461 gagcagttgg ggtatgatgg gcatgttagc cagccagcag aaccagtcag gcccatcggg
     2521 taataaccaa aaccaaggca acatgcagag ggagccaaac caggccttcg gttctggaaa
     2581 taactcttat agtggctcta attctggtgc agcaattggt tggggatcag catccaatgc
     2641 agggtcgggc agtggtttta atggaggctt tggctcaagc atggattcta agtcttctgg
     2701 ctggggaatg tagactagac ccagctttct tgtacaaagt ggttgatcct ctactagtaa
     2761 gcttgcatgc gatatctcta gagctgagaa cttcagggtg agtttgggga cccttgattg
     2821 ttctttcttt ttcgctattg taaaattcat gttatatgga gggggcaaag ttttcagggt
     2881 gttgtttaga atgggaagat gtcccttgta tcaccatgga ccctcatgat aattttgttt
     2941 ctttcacttt ctactctgtt gacaaccatt gtctcctctt attttctttt cattttctgt
     3001 aactttttcg ttaaacttta gcttgcattt gtaacgaatt tttaaattca cttttgttta
     3061 tttgtcagat tgtaagtact ttctctaatc actttttttt caaggcaatc agggtatatt
     3121 atattgtact tcagcacagt tttagagaac aattgttata attaaatgat aaggtagaat
     3181 atttctgcat ataaattctg gctggcgtgg aaatattctt attggtagaa acaactacac
     3241 cctggtcatc atcctgcctt tctctttatg gttacaatga tatacactgt ttgagatgag
     3301 gataaaatac tctgagtcca aaccgggccc ctctgctaac catgttcatg ccttcttctc
     3361 tttcctacag ctcctgggca acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga
     3421 attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct
     3481 ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa
     3541 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
     3601 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
     3661 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa
     3721 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
     3781 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt
     3841 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct
     3901 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc tcgactgcat
     3961 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     4021 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4081 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4141 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4201 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4261 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4321 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4381 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4441 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4501 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4561 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4621 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4681 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4741 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4801 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     4861 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4921 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     4981 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     5041 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     5101 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     5161 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     5221 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     5281 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     5341 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     5401 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     5461 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     5521 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc
     5581 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     5641 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     5701 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     5761 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     5821 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     5881 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     5941 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     6001 ccctttcgtc ttcactcgac gcggccagct tggtcgagct ggatacttcc cgtccgccag
     6061 ggggacatgc cggcgatgct gaaggtcgcg cgcattcccg atgaagaggc cggttaccgc
     6121 ctgttgacct ggtgggacgg gcagggcgc
//
