LOCUS       sequence-208283-        8404 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To test the effect of sequence on TDP43 splicing activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8404)
  TITLE     Rajat Rohatgi TDP43 plasmids
REFERENCE   2  (bases 1 to 8404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHBS1185 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 F-S]
FEATURES             Location/Qualifiers
     source          1..8404
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(261..280)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     530..548
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2170..2179
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        2332..2635
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2636..2839
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2790..2810
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2901..2910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2907..3608
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     misc_feature    3609..3674
                     /label=MCS
                     /note="multiple cloning site"
     CDS             3699..3755
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(5001..5025)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          5053..5625
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(5757..5776)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5822..5877
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5876..5895)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6275..6292)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6446..7034)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6526..6545)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7205..7996,7997..8065))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7828..7847
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8066..8170)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8238..8256
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catgccgccg gtggagtggc ggccctcggc gcgttcgtac tgttccacga tggtgtagtc
       61 ctcgttgtgg gaggtgatgt ccaacttgat gttgacgttg taggcgccgg gcagctgcac
      121 gggcttcttg gccttgtagg tggtcttgac ctcagcgtcg tagtggccgc cgtccttcag
      181 cttcagcctc tgcttgatct cgcccttcag ggcgccgtcc tcggggtaca tccgctcgga
      241 ggaggcctcc cagcccatgg tcttcttctg cattacgggg ccgtcggagg ggaagttggt
      301 gccgcgcagc ttcaccttgt agatgaactc gccgtcctgc agggaggagt cctgggtcac
      361 ggtcaccacg ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc cctcggggaa
      421 ggacagcttc aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct tggagccgta
      481 catgaactga ggggacagga tgtcccaggc gaagggcagg gggccaccct tggtcacctt
      541 cagcttggcg gtctgggtgc cctcgtaggg gcggccctga ggaaaaaaaa ggaaagggca
      601 tgctcgccat gtgcaagata cagtgttgaa tgtggtgcaa aatataaaga tgtggaagaa
      661 aaagccactg aaaataatat gaggaaataa aaaactagag taaattatca gtggaacaat
      721 ttctattata caagacacta cacccataca ttctcctaat gctcatgtaa gaatttctcc
      781 aaaaatacct tccagcacta caaactagaa aaaaaaagag acatggacac caaattaagt
      841 tcttaatagt gaagaacaaa agaactacct tgcctgctcc agtggatcca gcaaccgcca
      901 acaactgtcc tctttctatc ttgaaattaa tatctttcag gacaggagta ccaagaagtg
      961 agaaattact gaagaagagg ctgtcatcac cattagaagt ttttctattg ttattgtttt
     1021 gttttgcttt ctcaaattat tccccaaatc cctgttaaaa aaaaacacac acacacacac
     1081 acacacacac atcaaaaata aaagatgagt ttgtcagata ttttcaatag atgcttgttt
     1141 tatgtaccat actcacctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc
     1201 cctccatgtg caccttgaag cgcatgaact ccttgatgat ggccatgtta tcctcctcgc
     1261 ccttgctggt tccggtgcct ccactttcat cttcaccttt agtgataccg gctgccgtca
     1321 cgaactcctt caggaccatg tgatcgcgct tctcgttcgg gtctttgctc agcgtggtct
     1381 gggtgctcag atagtggttg tccggcaggc gcaccgggcc gtcgccgatc ggggtgttct
     1441 gctgtttgtg gtcggcttcc tgctggctgc cgtcctcaac gttgtggcgg atcttgaagt
     1501 tagccttgat gccgttcttc tgcttgtcgg cttcgatacg gacgttgtgg ctgttgtggt
     1561 tgtactccag cttgtgaccc aggatgttgc cgtcctcctt gaagtcgttg cccttcagct
     1621 cgatgcggtt caccagggtg tcgccctcga acttcacctc cgcgcgggtc ttgtaggtgc
     1681 cgtcgtcctt gaactcgatg gtgcgctcct ggacgtagcc ttccggcatg gcggacttga
     1741 agaagtcgtg acgcttcatg tggtccgggt agcggctgaa gcactgcacg ccgtaggtca
     1801 gggtggtcac gagggtcggc cacggcaccg gcagcttgcc ggtggtgcag atgaacttca
     1861 gggtcagctt gccctcggtg gcatcgccct cgccctcgcc acgcacgctg aacttgtggc
     1921 cgtttacgtc gccgtccagc tcgactttga taggcacagt acctgtaaat agttcctctc
     1981 ctttactggt accatcatca gggaatgagc ttacatttct cctcttcagc atctggtcat
     2041 ccttcttagc tttcctcagc tccacattga cctctattct gcgacgcctc atttctgtac
     2101 tgtcttttcc cttgttcttg aatctgtgaa gacgggcagc tggtgtatta gcattctcgt
     2161 tggtggatcc catggtggca ccggtgaatt ctccaggcga tctgacggtt cactaaacga
     2221 gctctgctta tataggcctc ccaccgtaca cgccacctcg acatactcga gtagttatta
     2281 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     2341 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     2401 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     2461 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     2521 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     2581 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     2641 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     2701 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     2761 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     2821 ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagggat cctctagtca
     2881 gctgacgcgt taccaccggt gccaccatgg gaagcgagct gattaaggag aacatgcaca
     2941 tgaagctgta catggagggc accgtggaca accatcactt caagtgcaca tccgagggcg
     3001 aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag ggcggccctc
     3061 tccccttcgc cttcgacatc ctggctacta gcttcctcta cggcagcaag accttcatca
     3121 accacaccca gggcatcccc gacttcttca agcagtcctt ccctgagggc ttcacatggg
     3181 agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc
     3241 aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttcaca tccaacggcc
     3301 ctgtgatgca gaagaaaaca ctcggctggg aggccttcac cgagacgctg taccccgctg
     3361 acggcggcct ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg agccatctga
     3421 tcgcaaacgc caagaccaca tatagatcca agaaacccgc taagaacctc aagatgcctg
     3481 gcgtctacta tgtggactac agactggaaa gaatcaagga ggccaacaac gagacctacg
     3541 tcgagcagca cgaggtggca gtggccagat actgcgacct ccctagcaaa ctggggcaca
     3601 agcttaattc cggactcaga tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg
     3661 cgggcccggg atccaccgga tctagaggag gaagcggagc tactaacttc agcctgctga
     3721 agcaggctgg agacgtggag gagaaccctg gacctatgtc tgaatatatt cgggtaaccg
     3781 aagatgagaa cgatgagccc attgaaatac catcggaaga cgatgggacg gtgctgctct
     3841 ccacggttac agcccagttt ccaggggcgt gtgggcttcg ctacaggaat ccagtgtctc
     3901 agtgtatgag aggtgtccgg ctggtagaag gaattctgca tgccccagat gctggctggg
     3961 gaaatctggt gtatgttgtc aactatccaa aagataacaa aagaaaaatg gatgagacag
     4021 atgcttcatc agcagtgaaa gtgaaaagag cagtccagaa aacatccgat ttaatagtgt
     4081 tgggtctccc atggaaaaca accgaacagg acctgaaaga gtattttagt acctttggag
     4141 aagttcttat ggtgcaggtc aagaaagatc ttaagactgg tcattcaaag gggtttggct
     4201 ttgttcgttt tacggaatat gaaacacaag tgaaagtaat gtcacagcga catatgatag
     4261 atggacgatg gtgtgactgc aaacttccta attctaagca aagccaagat gagcctttga
     4321 gaagcagaaa agtgtttgtg gggcgctgta cagaggacat gactgaggat gagctgcggg
     4381 agttcttctc tcagtacggg gatgtgatgg atgtcttcat ccccaagcca ttcagggcct
     4441 ttgcctttgt tacatttgca gatgatcaga ttgcgcagtc tctttgtgga gaggacttga
     4501 tcattaaagg aatcagcgtt catatatcca atgccgaacc taaacataat tcaaaccggc
     4561 agttggaaag atcggggcgc agtggtggca acccaggcgg ttctggtaat cagggcggct
     4621 cgggaaattc cagaggcggg ggtgcagggc ttggcaacaa tcaggggtcc aatatgggcg
     4681 gtggtatgaa ctcaggtgcc agctcgatta atccggccat gatggcggca gcgcaggcgg
     4741 cgctgcagtc ttcctggggt atgatgggta tgttagcatc ccaacagaac cagagtggcc
     4801 cgtcggggaa taaccaaaat caggggaata tgcagcgcga accgaatcaa gcttcaggta
     4861 gtggtaacaa cagttatagt ggatcgaaca gtggcgccgc aattggatgg ggttccgcat
     4921 ccaatgcagg ctcaggcagc ggctcgaatg gcggctcagg ttctagtatg gattcgaaga
     4981 gctctggttg gggaatgtag acccagcttt cttgtacaaa gtggttgatc ctctagagct
     5041 gagaacttca gggtgagttt ggggaccctt gattgttctt tctttttcgc tattgtaaaa
     5101 ttcatgttat atggaggggg caaagttttc agggtgttgt ttagaatggg aagatgtccc
     5161 ttgtatcacc atggaccctc atgataattt tgtttctttc actttctact ctgttgacaa
     5221 ccattgtctc ctcttatttt cttttcattt tctgtaactt tttcgttaaa ctttagcttg
     5281 catttgtaac gaatttttaa attcactttt gtttatttgt cagattgtaa gtactttctc
     5341 taatcacttt tttttcaagg caatcagggt atattatatt gtacttcagc acagttttag
     5401 agaacaattg ttataattaa atgataaggt agaatatttc tgcatataaa ttctggctgg
     5461 cgtggaaata ttcttattgg tagaaacaac tacaccctgg tcatcatcct gcctttctct
     5521 ttatggttac aatgatatac actgtttgag atgaggataa aatactctga gtccaaaccg
     5581 ggcccctctg ctaaccatgt tcatgccttc ttctctttcc tacagctcct gggcaacgtg
     5641 ctggttgttg tgctgtctca tcattttggc aaagaattca ctcctcaggt gcaggctgcc
     5701 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt
     5761 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
     5821 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
     5881 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
     5941 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
     6001 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     6061 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     6121 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
     6181 gtccctcttc tcttatggag atccctcgac tgcattaatg aatcggccaa cgcgcgggga
     6241 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6301 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6361 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6421 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6481 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6541 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6601 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6661 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6721 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6781 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6841 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     6901 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6961 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7021 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7081 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7141 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7201 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7261 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7321 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7381 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7441 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7501 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7561 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7621 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7681 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7741 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7801 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
     7861 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     7921 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     7981 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8041 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8101 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8161 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     8221 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcac tcgacgcggc
     8281 cagcttggtc gagctggata cttcccgtcc gccaggggga catgccggcg atgctgaagg
     8341 tcgcgcgcat tcccgatgaa gaggccggtt accgcctgtt gacctggtgg gacgggcagg
     8401 gcgc
//
