LOCUS       sequence-208284-        8378 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To test the effect of sequence on TDP43 splicing activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8378)
  TITLE     Rajat Rohatgi TDP43 plasmids
REFERENCE   2  (bases 1 to 8378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHBS1203 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 2x-Phi]
FEATURES             Location/Qualifiers
     source          1..8378
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(236..255)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     505..523
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2145..2154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        2307..2610
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2611..2814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2765..2785
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2876..2885
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2882..3583
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     misc_feature    3584..3649
                     /label=MCS
                     /note="multiple cloning site"
     CDS             3674..3730
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(4976..5000)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          5027..5599
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(5731..5750)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5796..5851
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5850..5869)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6249..6266)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6420..7008)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6500..6519)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7179..7970,7971..8039))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7802..7821
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8040..8144)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8212..8230
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcggcgcgtt cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt gatgtccaac
       61 ttgatgttga cgttgtaggc gccgggcagc tgcacgggct tcttggcctt gtaggtggtc
      121 ttgacctcag cgtcgtagtg gccgccgtcc ttcagcttca gcctctgctt gatctcgccc
      181 ttcagggcgc cgtcctcggg gtacatccgc tcggaggagg cctcccagcc catggtcttc
      241 ttctgcatta cggggccgtc ggaggggaag ttggtgccgc gcagcttcac cttgtagatg
      301 aactcgccgt cctgcaggga ggagtcctgg gtcacggtca ccacgccgcc gtcctcgaag
      361 ttcatcacgc gctcccactt gaagccctcg gggaaggaca gcttcaagta gtcggggatg
      421 tcggcggggt gcttcacgta ggccttggag ccgtacatga actgagggga caggatgtcc
      481 caggcgaagg gcagggggcc acccttggtc accttcagct tggcggtctg ggtgccctcg
      541 taggggcggc cctgaggaaa aaaaaggaaa gggcatgctc gccatgtgca agatacagtg
      601 ttgaatgtgg tgcaaaatat aaagatgtgg aagaaaaagc cactgaaaat aatatgagga
      661 aataaaaaac tagagtaaat tatcagtgga acaatttcta ttatacaaga cactacaccc
      721 atacattctc ctaatgctca tgtaagaatt tctccaaaaa taccttccag cactacaaac
      781 tagaaaaaaa aagagacatg gacaccaaat taagttctta atagtgaaga acaaaagaac
      841 taccttgcct gctccagtgg atccagcaac cgccaacaac tgtcctcttt ctatcttgaa
      901 attaatatct ttcaggacag gagtaccaag aagtgagaaa ttactgaaga agaggctgtc
      961 atcaccatta gaagtttttc tattgttatt gttttgtttt gctttctcaa attattcccc
     1021 aaatccctgt taaaaaaaaa cacacacaca cacacacaca cacacatcaa aaataaaaga
     1081 tgagtttgtc agatattttc aatagatgct tgttttatgt accatactca cctcgccctc
     1141 gccctcgatc tcgaactcgt ggccgttcac ggagccctcc atgtgcacct tgaagcgcat
     1201 gaactccttg atgatggcca tgttatcctc ctcgcccttg ctggttccgg tgcctccact
     1261 ttcatcttca cctttagtga taccggctgc cgtcacgaac tccttcagga ccatgtgatc
     1321 gcgcttctcg ttcgggtctt tgctcagcgt ggtctgggtg ctcagatagt ggttgtccgg
     1381 caggcgcacc gggccgtcgc cgatcggggt gttctgctgt ttgtggtcgg cttcctgctg
     1441 gctgccgtcc tcaacgttgt ggcggatctt gaagttagcc ttgatgccgt tcttctgctt
     1501 gtcggcttcg atacggacgt tgtggctgtt gtggttgtac tccagcttgt gacccaggat
     1561 gttgccgtcc tccttgaagt cgttgccctt cagctcgatg cggttcacca gggtgtcgcc
     1621 ctcgaacttc acctccgcgc gggtcttgta ggtgccgtcg tccttgaact cgatggtgcg
     1681 ctcctggacg tagccttccg gcatggcgga cttgaagaag tcgtgacgct tcatgtggtc
     1741 cgggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tcggccacgg
     1801 caccggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccct cggtggcatc
     1861 gccctcgccc tcgccacgca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
     1921 tttgataggc acagtacctg taaatagttc ctctccttta ctggtaccat catcagggaa
     1981 tgagcttaca tttctcctct tcagcatctg gtcatccttc ttagctttcc tcagctccac
     2041 attgacctct attctgcgac gcctcatttc tgtactgtct tttcccttgt tcttgaatct
     2101 gtgaagacgg gcagctggtg tattagcatt ctcgttggtg gatcccatgg tggcaccggt
     2161 gaattctcca ggcgatctga cggttcacta aacgagctct gcttatatag gcctcccacc
     2221 gtacacgcca cctcgacata ctcgagtagt tattaatagt aatcaattac ggggtcatta
     2281 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     2341 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     2401 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     2461 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     2521 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     2581 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2641 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2701 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2761 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta
     2821 gtgaaccgtc agatccgcta gggatcctct agtcagctga cgcgttacca ccggtgccac
     2881 catgggaagc gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt
     2941 ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca
     3001 gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc
     3061 tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt
     3121 cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg
     3181 gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt
     3241 caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg
     3301 ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga
     3361 catggccctg aagctcgtgg gcgggagcca tctgatcgca aacgccaaga ccacatatag
     3421 atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact
     3481 ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg tggcagtggc
     3541 cagatactgc gacctcccta gcaaactggg gcacaagctt aattccggac tcagatctcg
     3601 agctcaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggatctag
     3661 aggaggaagc ggagctacta acttcagcct gctgaagcag gctggagacg tggaggagaa
     3721 ccctggacct atgtctgaat atattcgggt aaccgaagat gagaacgatg agcccattga
     3781 aataccatcg gaagacgatg ggacggtgct gctctccacg gttacagccc agtttccagg
     3841 ggcgtgtggg cttcgctaca ggaatccagt gtctcagtgt atgagaggtg tccggctggt
     3901 agaaggaatt ctgcatgccc cagatgctgg ctggggaaat ctggtgtatg ttgtcaacta
     3961 tccaaaagat aacaaaagaa aaatggatga gacagatgct tcatcagcag tgaaagtgaa
     4021 aagagcagtc cagaaaacat ccgatttaat agtgttgggt ctcccatgga aaacaaccga
     4081 acaggacctg aaagagtatt ttagtacctt tggagaagtt cttatggtgc aggtcaagaa
     4141 agatcttaag actggtcatt caaaggggtt tggctttgtt cgttttacgg aatatgaaac
     4201 acaagtgaaa gtaatgtcac agcgacatat gatagatgga cgatggtgtg actgcaaact
     4261 tcctaattct aagcaaagcc aagatgagcc tttgagaagc agaaaagtgt ttgtggggcg
     4321 ctgtacagag gacatgactg aggatgagct gcgggagttc ttctctcagt acggggatgt
     4381 gatggatgtc ttcatcccca agccattcag ggcctttgcc tttgttacat ttgcagatga
     4441 tcagattgcg cagtctcttt gtggagagga cttgatcatt aaaggaatca gcgttcatat
     4501 atccaatgcc gaacctaaac ataatagcaa ccgacaactc gagagatctg gtagaggtaa
     4561 tccgaatcag aattcccgag gaggatttgg gggagggttt ggaggcgggt tcggccttgg
     4621 gatgggcttc ggtttttctg gagcgggcaa caatcagggc tcaaacggtg gtatgaacgc
     4681 aataaacccg gctatgatgg ctgcggctca ggccgcacta cagagcagtt ggggaatgat
     4741 gggcatgctg gctagtcagc agaatcagag tggtccgtca gggaacaacc agaaccaggg
     4801 aaatatgcag cgcgagccta atcaggctag cggaaacaat tcatttggtt attcaatcgg
     4861 atgggggggc tttgggggat ttggagggtg gggcatgtca ggcagtaata gcggagccgc
     4921 gagcgcttcc aatgcagggt caggttcgaa ttctatggac agcaaaagtt cgtagaccca
     4981 gctttcttgt acaaagtggt tgatcctcta gactgagaac ttcagggtga gtttggggac
     5041 ccttgattgt tctttctttt tcgctattgt aaaattcatg ttatatggag ggggcaaagt
     5101 tttcagggtg ttgtttagaa tgggaagatg tcccttgtat caccatggac cctcatgata
     5161 attttgtttc tttcactttc tactctgttg acaaccattg tctcctctta ttttcttttc
     5221 attttctgta actttttcgt taaactttag cttgcatttg taacgaattt ttaaattcac
     5281 ttttgtttat ttgtcagatt gtaagtactt tctctaatca cttttttttc aaggcaatca
     5341 gggtatatta tattgtactt cagcacagtt ttagagaaca attgttataa ttaaatgata
     5401 aggtagaata tttctgcata taaattctgg ctggcgtgga aatattctta ttggtagaaa
     5461 caactacacc ctggtcatca tcctgccttt ctctttatgg ttacaatgat atacactgtt
     5521 tgagatgagg ataaaatact ctgagtccaa accgggcccc tctgctaacc atgttcatgc
     5581 cttcttctct ttcctacagc tcctgggcaa cgtgctggtt gttgtgctgt ctcatcattt
     5641 tggcaaagaa ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc
     5701 caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga
     5761 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
     5821 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     5881 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc
     5941 tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
     6001 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt
     6061 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt
     6121 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct
     6181 cgactgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6241 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6301 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6361 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6421 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6481 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6541 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6601 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6661 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6721 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6781 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6841 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6901 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6961 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7021 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7081 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7141 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7201 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7261 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7321 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7381 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7441 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7501 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7561 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7621 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7681 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7741 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg
     7801 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7861 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7921 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7981 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     8041 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     8101 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8161 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     8221 atcacgaggc cctttcgtct tcactcgacg cggccagctt ggtcgagctg gatacttccc
     8281 gtccgccagg gggacatgcc ggcgatgctg aaggtcgcgc gcattcccga tgaagaggcc
     8341 ggttaccgcc tgttgacctg gtgggacggg cagggcgc
//
