LOCUS       sequence-208286-         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yellow fluorescent protein with improved photostability.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Wiens MD, Hoffmann F, Chen Y, Campbell RE
  TITLE     Enhancing fluorescent protein photostability through robot-assisted 
            photobleaching.
  JOURNAL   Integr Biol (Camb). 2018 Jul 16;10(7):419-428. doi: 
            10.1039/c8ib00063h.
  PUBMED    29897363
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Integr Biol (Camb)"; date: "2018-07-16- 16"; volume: "10"; issue: 
            "7"; pages: "419-428"
COMMENT     Sequence Label: pBAD-Citrine2
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..720)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVTGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLTCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNHNSHYVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSQLSKDPNEERDHTVLL
                     EFVTAAGITLGMGXLYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg accggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccttcggcta cggcctgacg tgcttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaaccacaa cagccactac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg cgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagct accagtccca gctgagcaaa gaccccaacg aggagcgcga tcacacggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tgggcganct gtacaagtaa
//
