LOCUS       sequence-208296-        8390 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To test the effect of sequence on TDP43 splicing activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8390)
  TITLE     Rajat Rohatgi TDP43 plasmids
REFERENCE   2  (bases 1 to 8390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHBS1202 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 4x-Phi]
FEATURES             Location/Qualifiers
     source          1..8390
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(247..266)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     516..534
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2156..2165
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        2318..2621
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2622..2825
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2776..2796
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2887..2896
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2893..3594
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     misc_feature    3595..3660
                     /label=MCS
                     /note="multiple cloning site"
     CDS             3685..3741
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(4987..5011)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          5039..5611
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(5743..5762)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5808..5863
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5862..5881)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6261..6278)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6432..7020)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6512..6531)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7191..7982,7983..8051))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7814..7833
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8052..8156)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8224..8242
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg
       61 tgatgtccaa cttgatgttg acgttgtagg cgccgggcag ctgcacgggc ttcttggcct
      121 tgtaggtggt cttgacctca gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct
      181 tgatctcgcc cttcagggcg ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc
      241 ccatggtctt cttctgcatt acggggccgt cggaggggaa gttggtgccg cgcagcttca
      301 ccttgtagat gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc accacgccgc
      361 cgtcctcgaa gttcatcacg cgctcccact tgaagccctc ggggaaggac agcttcaagt
      421 agtcggggat gtcggcgggg tgcttcacgt aggccttgga gccgtacatg aactgagggg
      481 acaggatgtc ccaggcgaag ggcagggggc cacccttggt caccttcagc ttggcggtct
      541 gggtgccctc gtaggggcgg ccctgaggaa aaaaaaggaa agggcatgct cgccatgtgc
      601 aagatacagt gttgaatgtg gtgcaaaata taaagatgtg gaagaaaaag ccactgaaaa
      661 taatatgagg aaataaaaaa ctagagtaaa ttatcagtgg aacaatttct attatacaag
      721 acactacacc catacattct cctaatgctc atgtaagaat ttctccaaaa ataccttcca
      781 gcactacaaa ctagaaaaaa aaagagacat ggacaccaaa ttaagttctt aatagtgaag
      841 aacaaaagaa ctaccttgcc tgctccagtg gatccagcaa ccgccaacaa ctgtcctctt
      901 tctatcttga aattaatatc tttcaggaca ggagtaccaa gaagtgagaa attactgaag
      961 aagaggctgt catcaccatt agaagttttt ctattgttat tgttttgttt tgctttctca
     1021 aattattccc caaatccctg ttaaaaaaaa acacacacac acacacacac acacacatca
     1081 aaaataaaag atgagtttgt cagatatttt caatagatgc ttgttttatg taccatactc
     1141 acctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc
     1201 ttgaagcgca tgaactcctt gatgatggcc atgttatcct cctcgccctt gctggttccg
     1261 gtgcctccac tttcatcttc acctttagtg ataccggctg ccgtcacgaa ctccttcagg
     1321 accatgtgat cgcgcttctc gttcgggtct ttgctcagcg tggtctgggt gctcagatag
     1381 tggttgtccg gcaggcgcac cgggccgtcg ccgatcgggg tgttctgctg tttgtggtcg
     1441 gcttcctgct ggctgccgtc ctcaacgttg tggcggatct tgaagttagc cttgatgccg
     1501 ttcttctgct tgtcggcttc gatacggacg ttgtggctgt tgtggttgta ctccagcttg
     1561 tgacccagga tgttgccgtc ctccttgaag tcgttgccct tcagctcgat gcggttcacc
     1621 agggtgtcgc cctcgaactt cacctccgcg cgggtcttgt aggtgccgtc gtccttgaac
     1681 tcgatggtgc gctcctggac gtagccttcc ggcatggcgg acttgaagaa gtcgtgacgc
     1741 ttcatgtggt ccgggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg
     1801 gtcggccacg gcaccggcag cttgccggtg gtgcagatga acttcagggt cagcttgccc
     1861 tcggtggcat cgccctcgcc ctcgccacgc acgctgaact tgtggccgtt tacgtcgccg
     1921 tccagctcga ctttgatagg cacagtacct gtaaatagtt cctctccttt actggtacca
     1981 tcatcaggga atgagcttac atttctcctc ttcagcatct ggtcatcctt cttagctttc
     2041 ctcagctcca cattgacctc tattctgcga cgcctcattt ctgtactgtc ttttcccttg
     2101 ttcttgaatc tgtgaagacg ggcagctggt gtattagcat tctcgttggt ggatcccatg
     2161 gtggcaccgg tgaattctcc aggcgatctg acggttcact aaacgagctc tgcttatata
     2221 ggcctcccac cgtacacgcc acctcgacat actcgagtag ttattaatag taatcaatta
     2281 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     2341 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     2401 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     2461 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     2521 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     2581 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     2641 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     2701 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     2761 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     2821 gagctggttt agtgaaccgt cagatccgct agggatcctc tagtcagctg acgcgttacc
     2881 accggtgcca ccatgggaag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg
     2941 gagggcaccg tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac
     3001 gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc
     3061 gacatcctgg ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc
     3121 atccccgact tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca
     3181 tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc
     3241 atctacaacg tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag
     3301 aaaacactcg gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa
     3361 ggcagaaacg acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacgccaag
     3421 accacatata gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg
     3481 gactacagac tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag
     3541 gtggcagtgg ccagatactg cgacctccct agcaaactgg ggcacaagct taattccgga
     3601 ctcagatctc gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc
     3661 accggatcta gaggaggaag cggagctact aacttcagcc tgctgaagca ggctggagac
     3721 gtggaggaga accctggacc tatgtctgaa tatattcggg taaccgaaga tgagaacgat
     3781 gagcccattg aaataccatc ggaagacgat gggacggtgc tgctctccac ggttacagcc
     3841 cagtttccag gggcgtgtgg gcttcgctac aggaatccag tgtctcagtg tatgagaggt
     3901 gtccggctgg tagaaggaat tctgcatgcc ccagatgctg gctggggaaa tctggtgtat
     3961 gttgtcaact atccaaaaga taacaaaaga aaaatggatg agacagatgc ttcatcagca
     4021 gtgaaagtga aaagagcagt ccagaaaaca tccgatttaa tagtgttggg tctcccatgg
     4081 aaaacaaccg aacaggacct gaaagagtat tttagtacct ttggagaagt tcttatggtg
     4141 caggtcaaga aagatcttaa gactggtcat tcaaaggggt ttggctttgt tcgttttacg
     4201 gaatatgaaa cacaagtgaa agtaatgtca cagcgacata tgatagatgg acgatggtgt
     4261 gactgcaaac ttcctaattc taagcaaagc caagatgagc ctttgagaag cagaaaagtg
     4321 tttgtggggc gctgtacaga ggacatgact gaggatgagc tgcgggagtt cttctctcag
     4381 tacggggatg tgatggatgt cttcatcccc aagccattca gggcctttgc ctttgttaca
     4441 tttgcagatg atcagattgc gcagtctctt tgtggagagg acttgatcat taaaggaatc
     4501 agcgttcata tatccaatgc cgaacctaaa cataacagta accgccagct ggaacgcagc
     4561 ggcaggggca atcctttcgg cggtggtttc ggcggcggct tcggtaacca gaatagtaga
     4621 ggtggaggcg ccgggaataa ccagggtagt aacttaggaa tgggcttcgg attctcagga
     4681 ggtatgaatg ccattaatcc cgcgatgatg gcggcggcac aggcggcctt gcagagctcc
     4741 tggggaatga tgggcatgct ggcatcccag cagaatcaat caggtccgtc tggaaacaat
     4801 cagaatcagg gaaatatgca gcgggaaccg aaccaagcct caggcaataa ttcctttggt
     4861 tatagcatcg ggtggggcgg tagcaatagc ggggccgcta gcgcctcaaa tgccggttca
     4921 ggctcaaatg ggtttggagg atttggtgga tggggaatgt ctagtatgga ttcgaaatca
     4981 agctagaccc agctttcttg tacaaagtgg ttgatcctct agagctgaga acttcagggt
     5041 gagtttgggg acccttgatt gttctttctt tttcgctatt gtaaaattca tgttatatgg
     5101 agggggcaaa gttttcaggg tgttgtttag aatgggaaga tgtcccttgt atcaccatgg
     5161 accctcatga taattttgtt tctttcactt tctactctgt tgacaaccat tgtctcctct
     5221 tattttcttt tcattttctg taactttttc gttaaacttt agcttgcatt tgtaacgaat
     5281 ttttaaattc acttttgttt atttgtcaga ttgtaagtac tttctctaat cacttttttt
     5341 tcaaggcaat cagggtatat tatattgtac ttcagcacag ttttagagaa caattgttat
     5401 aattaaatga taaggtagaa tatttctgca tataaattct ggctggcgtg gaaatattct
     5461 tattggtaga aacaactaca ccctggtcat catcctgcct ttctctttat ggttacaatg
     5521 atatacactg tttgagatga ggataaaata ctctgagtcc aaaccgggcc cctctgctaa
     5581 ccatgttcat gccttcttct ctttcctaca gctcctgggc aacgtgctgg ttgttgtgct
     5641 gtctcatcat tttggcaaag aattcactcc tcaggtgcag gctgcctatc agaaggtggt
     5701 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa
     5761 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
     5821 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     5881 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
     5941 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
     6001 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
     6061 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
     6121 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
     6181 atggagatcc ctcgactgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     6241 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     6301 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     6361 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6421 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6481 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6541 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6601 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     6661 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6721 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6781 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6841 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     6901 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     6961 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7021 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7081 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7141 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7201 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7261 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7321 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7381 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7441 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7501 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7561 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7621 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     7681 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     7741 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7801 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     7861 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     7921 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     7981 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8041 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8101 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8161 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     8221 aaaaataggc gtatcacgag gccctttcgt cttcactcga cgcggccagc ttggtcgagc
     8281 tggatacttc ccgtccgcca gggggacatg ccggcgatgc tgaaggtcgc gcgcattccc
     8341 gatgaagagg ccggttaccg cctgttgacc tggtgggacg ggcagggcgc
//
