LOCUS       sequence-208298-        8411 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To test the effect of sequence on TDP43 splicing activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8411)
  TITLE     Rajat Rohatgi TDP43 plasmids
REFERENCE   2  (bases 1 to 8411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHBS1187 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 N-Q]
FEATURES             Location/Qualifiers
     source          1..8411
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(268..287)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     537..555
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2177..2186
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        2339..2642
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2643..2846
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2797..2817
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2908..2917
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2914..3615
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     misc_feature    3616..3681
                     /label=MCS
                     /note="multiple cloning site"
     CDS             3706..3762
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(5008..5032)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          5060..5632
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(5764..5783)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5829..5884
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5883..5902)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6282..6299)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6453..7041)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6533..6552)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7212..8003,8004..8072))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7835..7854
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8073..8177)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8245..8263
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gctcgtccat gccgccggtg gagtggcggc cctcggcgcg ttcgtactgt tccacgatgg
       61 tgtagtcctc gttgtgggag gtgatgtcca acttgatgtt gacgttgtag gcgccgggca
      121 gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc agcgtcgtag tggccgccgt
      181 ccttcagctt cagcctctgc ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc
      241 gctcggagga ggcctcccag cccatggtct tcttctgcat tacggggccg tcggagggga
      301 agttggtgcc gcgcagcttc accttgtaga tgaactcgcc gtcctgcagg gaggagtcct
      361 gggtcacggt caccacgccg ccgtcctcga agttcatcac gcgctcccac ttgaagccct
      421 cggggaagga cagcttcaag tagtcgggga tgtcggcggg gtgcttcacg taggccttgg
      481 agccgtacat gaactgaggg gacaggatgt cccaggcgaa gggcaggggg ccacccttgg
      541 tcaccttcag cttggcggtc tgggtgccct cgtaggggcg gccctgagga aaaaaaagga
      601 aagggcatgc tcgccatgtg caagatacag tgttgaatgt ggtgcaaaat ataaagatgt
      661 ggaagaaaaa gccactgaaa ataatatgag gaaataaaaa actagagtaa attatcagtg
      721 gaacaatttc tattatacaa gacactacac ccatacattc tcctaatgct catgtaagaa
      781 tttctccaaa aataccttcc agcactacaa actagaaaaa aaaagagaca tggacaccaa
      841 attaagttct taatagtgaa gaacaaaaga actaccttgc ctgctccagt ggatccagca
      901 accgccaaca actgtcctct ttctatcttg aaattaatat ctttcaggac aggagtacca
      961 agaagtgaga aattactgaa gaagaggctg tcatcaccat tagaagtttt tctattgtta
     1021 ttgttttgtt ttgctttctc aaattattcc ccaaatccct gttaaaaaaa aacacacaca
     1081 cacacacaca cacacacatc aaaaataaaa gatgagtttg tcagatattt tcaatagatg
     1141 cttgttttat gtaccatact cacctcgccc tcgccctcga tctcgaactc gtggccgttc
     1201 acggagccct ccatgtgcac cttgaagcgc atgaactcct tgatgatggc catgttatcc
     1261 tcctcgccct tgctggttcc ggtgcctcca ctttcatctt cacctttagt gataccggct
     1321 gccgtcacga actccttcag gaccatgtga tcgcgcttct cgttcgggtc tttgctcagc
     1381 gtggtctggg tgctcagata gtggttgtcc ggcaggcgca ccgggccgtc gccgatcggg
     1441 gtgttctgct gtttgtggtc ggcttcctgc tggctgccgt cctcaacgtt gtggcggatc
     1501 ttgaagttag ccttgatgcc gttcttctgc ttgtcggctt cgatacggac gttgtggctg
     1561 ttgtggttgt actccagctt gtgacccagg atgttgccgt cctccttgaa gtcgttgccc
     1621 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctccgc gcgggtcttg
     1681 taggtgccgt cgtccttgaa ctcgatggtg cgctcctgga cgtagccttc cggcatggcg
     1741 gacttgaaga agtcgtgacg cttcatgtgg tccgggtagc ggctgaagca ctgcacgccg
     1801 taggtcaggg tggtcacgag ggtcggccac ggcaccggca gcttgccggt ggtgcagatg
     1861 aacttcaggg tcagcttgcc ctcggtggca tcgccctcgc cctcgccacg cacgctgaac
     1921 ttgtggccgt ttacgtcgcc gtccagctcg actttgatag gcacagtacc tgtaaatagt
     1981 tcctctcctt tactggtacc atcatcaggg aatgagctta catttctcct cttcagcatc
     2041 tggtcatcct tcttagcttt cctcagctcc acattgacct ctattctgcg acgcctcatt
     2101 tctgtactgt cttttccctt gttcttgaat ctgtgaagac gggcagctgg tgtattagca
     2161 ttctcgttgg tggatcccat ggtggcaccg gtgaattctc caggcgatct gacggttcac
     2221 taaacgagct ctgcttatat aggcctccca ccgtacacgc cacctcgaca tactcgagta
     2281 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     2341 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     2401 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     2461 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     2521 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     2581 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     2641 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     2701 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     2761 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     2821 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagggatcct
     2881 ctagtcagct gacgcgttac caccggtgcc accatgggaa gcgagctgat taaggagaac
     2941 atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa gtgcacatcc
     3001 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc
     3061 ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg cagcaagacc
     3121 ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc tgagggcttc
     3181 acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc
     3241 agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa cttcacatcc
     3301 aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga gacgctgtac
     3361 cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt gggcgggagc
     3421 catctgatcg caaacgccaa gaccacatat agatccaaga aacccgctaa gaacctcaag
     3481 atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc caacaacgag
     3541 acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc tagcaaactg
     3601 gggcacaagc ttaattccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac
     3661 ggtaccgcgg gcccgggatc caccggatct agaggaggaa gcggagctac taacttcagc
     3721 ctgctgaagc aggctggaga cgtggaggag aaccctggac ctatgtctga atatattcgg
     3781 gtaaccgaag atgagaacga tgagcccatt gaaataccat cggaagacga tgggacggtg
     3841 ctgctctcca cggttacagc ccagtttcca ggggcgtgtg ggcttcgcta caggaatcca
     3901 gtgtctcagt gtatgagagg tgtccggctg gtagaaggaa ttctgcatgc cccagatgct
     3961 ggctggggaa atctggtgta tgttgtcaac tatccaaaag ataacaaaag aaaaatggat
     4021 gagacagatg cttcatcagc agtgaaagtg aaaagagcag tccagaaaac atccgattta
     4081 atagtgttgg gtctcccatg gaaaacaacc gaacaggacc tgaaagagta ttttagtacc
     4141 tttggagaag ttcttatggt gcaggtcaag aaagatctta agactggtca ttcaaagggg
     4201 tttggctttg ttcgttttac ggaatatgaa acacaagtga aagtaatgtc acagcgacat
     4261 atgatagatg gacgatggtg tgactgcaaa cttcctaatt ctaagcaaag ccaagatgag
     4321 cctttgagaa gcagaaaagt gtttgtgggg cgctgtacag aggacatgac tgaggatgag
     4381 ctgcgggagt tcttctctca gtacggggat gtgatggatg tcttcatccc caagccattc
     4441 agggcctttg cctttgttac atttgcagat gatcagattg cgcagtctct ttgtggagag
     4501 gacttgatca ttaaaggaat cagcgttcat atatccaatg ccgaacctaa acaccaatct
     4561 caacgccagc tcgaaagatc cggaaggttc ggcggccagc cgggggggtt cggtcagcag
     4621 ggaggatttg gtcaaagtcg cggtggaggc gcgggattgg gtcaacagca aggtagtcag
     4681 atgggtggcg gcatgcagtt tggcgccttc agtatccaac cggccatgat ggcggctgct
     4741 caggcggcgc ttcaatcgag ttggggtatg atggggatgt tggccagcca gcaaaatcag
     4801 tcagggcctt cgggacaaca gcagcaacag ggtcagatgc aacgtgagcc tcagcaagcc
     4861 tttggtagcg ggcaacagtc ttactcgggg tcccagtccg gagcggcgat tggttggggt
     4921 tcggccagtc aggcgggctc tgggtcaggg ttccagggcg gattcgggtc cagcatggat
     4981 tcaaagtcaa gcggatgggg tatgtagacc cagctttctt gtacaaagtg gttgatcctc
     5041 tagagctgag aacttcaggg tgagtttggg gacccttgat tgttctttct ttttcgctat
     5101 tgtaaaattc atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag
     5161 atgtcccttg tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg
     5221 ttgacaacca ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt
     5281 tagcttgcat ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta
     5341 ctttctctaa tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca
     5401 gttttagaga acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc
     5461 tggctggcgt ggaaatattc ttattggtag aaacaactac accctggtca tcatcctgcc
     5521 tttctcttta tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc
     5581 caaaccgggc ccctctgcta accatgttca tgccttcttc tctttcctac agctcctggg
     5641 caacgtgctg gttgttgtgc tgtctcatca ttttggcaaa gaattcactc ctcaggtgca
     5701 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
     5761 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     5821 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     5881 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     5941 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
     6001 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     6061 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
     6121 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
     6181 catagctgtc cctcttctct tatggagatc cctcgactgc attaatgaat cggccaacgc
     6241 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     6301 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     6361 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     6421 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6481 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6541 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6601 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     6661 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     6721 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     6781 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     6841 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     6901 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     6961 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7021 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     7081 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     7141 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7201 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7261 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     7321 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     7381 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     7441 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     7501 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     7561 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     7621 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     7681 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     7741 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     7801 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
     7861 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     7921 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     7981 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     8041 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     8101 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     8161 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     8221 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcactcg
     8281 acgcggccag cttggtcgag ctggatactt cccgtccgcc agggggacat gccggcgatg
     8341 ctgaaggtcg cgcgcattcc cgatgaagag gccggttacc gcctgttgac ctggtgggac
     8401 gggcagggcg c
//
