LOCUS       sequence-208299-        6408 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To test the effect of sequence on TDP43 splicing activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6408)
  TITLE     Rajat Rohatgi TDP43 plasmids
REFERENCE   2  (bases 1 to 6408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHBS1335 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G2...
FEATURES             Location/Qualifiers
     source          1..6408
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      175..184
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        337..640
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        641..844
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     795..815
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      906..915
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             912..1613
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     misc_feature    1614..1679
                     /label=MCS
                     /note="multiple cloning site"
     CDS             1704..1760
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(3006..3030)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          3057..3629
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(3761..3780)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3826..3881
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3880..3899)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4279..4296)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4450..5038)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4530..4549)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5209..6000,6001..6069))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5832..5851
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6070..6174)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6242..6260
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catcagggaa tgagcttaca tttctcctct tcagcatctg gtcatccttc ttagctttcc
       61 tcagctccac attgacctct attctgcgac gcctcatttc tgtactgtct tttcccttgt
      121 tcttgaatct gtgaagacgg gcagctggtg tattagcatt ctcgttggtg gatcccatgg
      181 tggcaccggt gaattctcca ggcgatctga cggttcacta aacgagctct gcttatatag
      241 gcctcccacc gtacacgcca cctcgacata ctcgagtagt tattaatagt aatcaattac
      301 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      361 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      421 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      481 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      541 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      601 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      661 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      721 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      781 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      841 agctggttta gtgaaccgtc agatccgcta gggatcctct agtcagctga cgcgttacca
      901 ccggtgccac catgggaagc gagctgatta aggagaacat gcacatgaag ctgtacatgg
      961 agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg
     1021 agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg
     1081 acatcctggc tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca
     1141 tccccgactt cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat
     1201 acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca
     1261 tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga
     1321 aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag
     1381 gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca aacgccaaga
     1441 ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg
     1501 actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg
     1561 tggcagtggc cagatactgc gacctcccta gcaaactggg gcacaagctt aattccggac
     1621 tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatcca
     1681 ccggatctag aggaggaagc ggagctacta acttcagcct gctgaagcag gctggagacg
     1741 tggaggagaa ccctggacct atgtctgaat atattcgggt aaccgaagat gagaacgatg
     1801 agcccattga aataccatcg gaagacgatg ggacggtgct gctctccacg gttacagccc
     1861 agtttccagg ggcgtgtggg cttcgctaca ggaatccagt gtctcagtgt atgagaggtg
     1921 tccggctggt agaaggaatt ctgcatgccc cagatgctgg ctggggaaat ctggtgtatg
     1981 ttgtcaacta tccaaaagat aacaaaagaa aaatggatga gacagatgct tcatcagcag
     2041 tgaaagtgaa aagagcagtc cagaaaacat ccgatttaat agtgttgggt ctcccatgga
     2101 aaacaaccga acaggacctg aaagagtatt ttagtacctt tggagaagtt cttatggtgc
     2161 aggtcaagaa agatcttaag actggtcatt caaaggggtt tggctttgtt cgttttacgg
     2221 aatatgaaac acaagtgaaa gtaatgtcac agcgacatat gatagatgga cgatggtgtg
     2281 actgcaaact tcctaattct aagcaaagcc aagatgagcc tttgagaagc agaaaagtgt
     2341 ttgtggggcg ctgtacagag gacatgactg aggatgagct gcgggagttc ttctctcagt
     2401 acggggatgt gatggatgtc ttcatcccca agccattcag ggcctttgcc tttgttacat
     2461 ttgcagatga tcagattgcg cagtctcttt gtggagagga cttgatcatt aaaggaatca
     2521 gcgttcatat atccaatgcc gaacctaagc acaatagcaa tagacagtta gaaagaagtg
     2581 gaagatttgg tggtaatcca ggtggctttg ggaatcaggg tggatttggt aatagcagag
     2641 ggtctggagc tggtttggga aacaatcaag gtagtaatat gggtggtggg atgaactttg
     2701 gtgcgttcag cattaatcca gccatgatgg ctgccgccca ggcagcacta cagagcagtt
     2761 ggggtatgat gggcatgtta gccagccagc agaaccagtc aggcccatcg ggtaataacc
     2821 aaaaccaagg caacatgcag agggagccaa accaggcctt cggttctgga aataactctt
     2881 atagtggctc taattctggt gcagcaattg gttggggatc agcatccaat gcagggtcgg
     2941 gcagtggttt taatggaggc tttggctcaa gcatggattc taagtcttct ggctggggaa
     3001 tgtagaccca gctttcttgt acaaagtggt tgatcctcta gactgagaac ttcagggtga
     3061 gtttggggac ccttgattgt tctttctttt tcgctattgt aaaattcatg ttatatggag
     3121 ggggcaaagt tttcagggtg ttgtttagaa tgggaagatg tcccttgtat caccatggac
     3181 cctcatgata attttgtttc tttcactttc tactctgttg acaaccattg tctcctctta
     3241 ttttcttttc attttctgta actttttcgt taaactttag cttgcatttg taacgaattt
     3301 ttaaattcac ttttgtttat ttgtcagatt gtaagtactt tctctaatca cttttttttc
     3361 aaggcaatca gggtatatta tattgtactt cagcacagtt ttagagaaca attgttataa
     3421 ttaaatgata aggtagaata tttctgcata taaattctgg ctggcgtgga aatattctta
     3481 ttggtagaaa caactacacc ctggtcatca tcctgccttt ctctttatgg ttacaatgat
     3541 atacactgtt tgagatgagg ataaaatact ctgagtccaa accgggcccc tctgctaacc
     3601 atgttcatgc cttcttctct ttcctacagc tcctgggcaa cgtgctggtt gttgtgctgt
     3661 ctcatcattt tggcaaagaa ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg
     3721 ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa
     3781 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta
     3841 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     3901 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc
     3961 atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc
     4021 cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta
     4081 tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac
     4141 tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat
     4201 ggagatccct cgactgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     4261 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     4321 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     4381 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     4441 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     4501 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     4561 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4621 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4681 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4741 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     4801 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     4861 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     4921 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     4981 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5041 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     5101 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     5161 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     5221 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5281 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5341 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5401 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     5461 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5521 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     5581 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     5641 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     5701 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     5761 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     5821 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     5881 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     5941 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6001 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6061 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     6121 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     6181 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     6241 aaataggcgt atcacgaggc cctttcgtct tcactcgacg cggccagctt ggtcgagctg
     6301 gatacttccc gtccgccagg gggacatgcc ggcgatgctg aaggtcgcgc gcattcccga
     6361 tgaagaggcc ggttaccgcc tgttgacctg gtgggacggg cagggcgc
//
