LOCUS       sequence-208301-        8386 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To test the effect of sequence on TDP43 splicing activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8386)
  TITLE     Rajat Rohatgi TDP43 plasmids
REFERENCE   2  (bases 1 to 8386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHBS1339 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G3...
FEATURES             Location/Qualifiers
     source          1..8386
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(244..263)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     513..531
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2153..2162
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        2315..2618
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2619..2822
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2773..2793
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2884..2893
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2890..3591
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     misc_feature    3592..3657
                     /label=MCS
                     /note="multiple cloning site"
     CDS             3682..3738
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(4984..5008)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          5035..5607
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(5739..5758)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5804..5859
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5858..5877)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6257..6274)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6428..7016)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6508..6527)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7187..7978,7979..8047))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7810..7829
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8048..8152)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8220..8238
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggcggccctc ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga
       61 tgtccaactt gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc ttggccttgt
      121 aggtggtctt gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga
      181 tctcgccctt cagggcgccg tcctcggggt acatccgctc ggaggaggcc tcccagccca
      241 tggtcttctt ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct
      301 tgtagatgaa ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc acgccgccgt
      361 cctcgaagtt catcacgcgc tcccacttga agccctcggg gaaggacagc ttcaagtagt
      421 cggggatgtc ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac tgaggggaca
      481 ggatgtccca ggcgaagggc agggggccac ccttggtcac cttcagcttg gcggtctggg
      541 tgccctcgta ggggcggccc tgaggaaaaa aaaggaaagg gcatgctcgc catgtgcaag
      601 atacagtgtt gaatgtggtg caaaatataa agatgtggaa gaaaaagcca ctgaaaataa
      661 tatgaggaaa taaaaaacta gagtaaatta tcagtggaac aatttctatt atacaagaca
      721 ctacacccat acattctcct aatgctcatg taagaatttc tccaaaaata ccttccagca
      781 ctacaaacta gaaaaaaaaa gagacatgga caccaaatta agttcttaat agtgaagaac
      841 aaaagaacta ccttgcctgc tccagtggat ccagcaaccg ccaacaactg tcctctttct
      901 atcttgaaat taatatcttt caggacagga gtaccaagaa gtgagaaatt actgaagaag
      961 aggctgtcat caccattaga agtttttcta ttgttattgt tttgttttgc tttctcaaat
     1021 tattccccaa atccctgtta aaaaaaaaca cacacacaca cacacacaca cacatcaaaa
     1081 ataaaagatg agtttgtcag atattttcaa tagatgcttg ttttatgtac catactcacc
     1141 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg
     1201 aagcgcatga actccttgat gatggccatg ttatcctcct cgcccttgct ggttccggtg
     1261 cctccacttt catcttcacc tttagtgata ccggctgccg tcacgaactc cttcaggacc
     1321 atgtgatcgc gcttctcgtt cgggtctttg ctcagcgtgg tctgggtgct cagatagtgg
     1381 ttgtccggca ggcgcaccgg gccgtcgccg atcggggtgt tctgctgttt gtggtcggct
     1441 tcctgctggc tgccgtcctc aacgttgtgg cggatcttga agttagcctt gatgccgttc
     1501 ttctgcttgt cggcttcgat acggacgttg tggctgttgt ggttgtactc cagcttgtga
     1561 cccaggatgt tgccgtcctc cttgaagtcg ttgcccttca gctcgatgcg gttcaccagg
     1621 gtgtcgccct cgaacttcac ctccgcgcgg gtcttgtagg tgccgtcgtc cttgaactcg
     1681 atggtgcgct cctggacgta gccttccggc atggcggact tgaagaagtc gtgacgcttc
     1741 atgtggtccg ggtagcggct gaagcactgc acgccgtagg tcagggtggt cacgagggtc
     1801 ggccacggca ccggcagctt gccggtggtg cagatgaact tcagggtcag cttgccctcg
     1861 gtggcatcgc cctcgccctc gccacgcacg ctgaacttgt ggccgtttac gtcgccgtcc
     1921 agctcgactt tgataggcac agtacctgta aatagttcct ctcctttact ggtaccatca
     1981 tcagggaatg agcttacatt tctcctcttc agcatctggt catccttctt agctttcctc
     2041 agctccacat tgacctctat tctgcgacgc ctcatttctg tactgtcttt tcccttgttc
     2101 ttgaatctgt gaagacgggc agctggtgta ttagcattct cgttggtgga tcccatggtg
     2161 gcaccggtga attctccagg cgatctgacg gttcactaaa cgagctctgc ttatataggc
     2221 ctcccaccgt acacgccacc tcgacatact cgagtagtta ttaatagtaa tcaattacgg
     2281 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     2341 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     2401 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     2461 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     2521 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     2581 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     2641 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     2701 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     2761 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     2821 ctggtttagt gaaccgtcag atccgctagg gatcctctag tcagctgacg cgttaccacc
     2881 ggtgccacca tgggaagcga gctgattaag gagaacatgc acatgaagct gtacatggag
     2941 ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
     3001 ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
     3061 atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc
     3121 cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
     3181 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
     3241 tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa
     3301 acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc
     3361 agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa cgccaagacc
     3421 acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac
     3481 tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg
     3541 gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa ttccggactc
     3601 agatctcgag ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc
     3661 ggatctagag gaggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg
     3721 gaggagaacc ctggacctat gtctgaatat attcgggtaa ccgaagatga gaacgatgag
     3781 cccattgaaa taccatcgga agacgatggg acggtgctgc tctccacggt tacagcccag
     3841 tttccagggg cgtgtgggct tcgctacagg aatccagtgt ctcagtgtat gagaggtgtc
     3901 cggctggtag aaggaattct gcatgcccca gatgctggct ggggaaatct ggtgtatgtt
     3961 gtcaactatc caaaagataa caaaagaaaa atggatgaga cagatgcttc atcagcagtg
     4021 aaagtgaaaa gagcagtcca gaaaacatcc gatttaatag tgttgggtct cccatggaaa
     4081 acaaccgaac aggacctgaa agagtatttt agtacctttg gagaagttct tatggtgcag
     4141 gtcaagaaag atcttaagac tggtcattca aaggggtttg gctttgttcg ttttacggaa
     4201 tatgaaacac aagtgaaagt aatgtcacag cgacatatga tagatggacg atggtgtgac
     4261 tgcaaacttc ctaattctaa gcaaagccaa gatgagcctt tgagaagcag aaaagtgttt
     4321 gtggggcgct gtacagagga catgactgag gatgagctgc gggagttctt ctctcagtac
     4381 ggggatgtga tggatgtctt catccccaag ccattcaggg cctttgcctt tgttacattt
     4441 gcagatgatc agattgcgca gtctctttgt ggagaggact tgatcattaa aggaatcagc
     4501 gttcatatat ccaatgccga acctaagcac aatagcaata gacagttaga aagaagtgga
     4561 agatttggtg gtaatccagg tggctttggg aatcagggtg gatttggtaa tagcagaggg
     4621 ggtggagctg gtttgggaaa caatcaaggt agtaatatgg gtggtgggat gaactttggt
     4681 gcgttcagca ttaatccagc catgatggct gccgcccagg cagcactaca gagcagttgg
     4741 ggtatgatgg gcatgttagc cagccagcag aaccagtcaa gcccatcggg taataaccaa
     4801 aaccaaggca acatgcagag ggagccaaac caggccttcg gttctggaaa taactcttat
     4861 agtggctcta attctggtgc agcaattggt tggggatcag catccaatgc agggtcgggc
     4921 agtggtttta atggaggctt tggctcaagc atggattcta agtcttctgg ctggggaatg
     4981 tagacccagc tttcttgtac aaagtggttg atcctctaga ctgagaactt cagggtgagt
     5041 ttggggaccc ttgattgttc tttctttttc gctattgtaa aattcatgtt atatggaggg
     5101 ggcaaagttt tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca ccatggaccc
     5161 tcatgataat tttgtttctt tcactttcta ctctgttgac aaccattgtc tcctcttatt
     5221 ttcttttcat tttctgtaac tttttcgtta aactttagct tgcatttgta acgaattttt
     5281 aaattcactt ttgtttattt gtcagattgt aagtactttc tctaatcact tttttttcaa
     5341 ggcaatcagg gtatattata ttgtacttca gcacagtttt agagaacaat tgttataatt
     5401 aaatgataag gtagaatatt tctgcatata aattctggct ggcgtggaaa tattcttatt
     5461 ggtagaaaca actacaccct ggtcatcatc ctgcctttct ctttatggtt acaatgatat
     5521 acactgtttg agatgaggat aaaatactct gagtccaaac cgggcccctc tgctaaccat
     5581 gttcatgcct tcttctcttt cctacagctc ctgggcaacg tgctggttgt tgtgctgtct
     5641 catcattttg gcaaagaatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct
     5701 ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat
     5761 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
     5821 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     5881 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
     5941 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc
     6001 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata
     6061 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta
     6121 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg
     6181 agatccctcg actgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6241 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6301 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     6361 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6421 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6481 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     6541 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     6601 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     6661 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     6721 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     6781 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     6841 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     6901 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     6961 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7021 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7081 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7141 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7201 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7261 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7321 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7381 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     7441 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     7501 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     7561 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     7621 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     7681 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     7741 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     7801 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     7861 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     7921 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     7981 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8041 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8101 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8161 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     8221 ataggcgtat cacgaggccc tttcgtcttc actcgacgcg gccagcttgg tcgagctgga
     8281 tacttcccgt ccgccagggg gacatgccgg cgatgctgaa ggtcgcgcgc attcccgatg
     8341 aagaggccgg ttaccgcctg ttgacctggt gggacgggca gggcgc
//
