LOCUS       sequence-208304-        8398 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To test the effect of sequence on TDP43 splicing activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8398)
  TITLE     Rajat Rohatgi TDP43 plasmids
REFERENCE   2  (bases 1 to 8398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHBS1285 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G3...
FEATURES             Location/Qualifiers
     source          1..8398
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(251..270)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     520..538
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2160..2169
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        2322..2625
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2626..2829
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2780..2800
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2891..2900
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2897..3598
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     misc_feature    3599..3664
                     /label=MCS
                     /note="multiple cloning site"
     CDS             3689..3745
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(4996..5020)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          5047..5619
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(5751..5770)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5816..5871
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5870..5889)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6269..6286)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6440..7028)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6520..6539)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7199..7990,7991..8059))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7822..7841
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8060..8164)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8232..8250
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gtggagtggc ggccctcggc gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg
       61 gaggtgatgt ccaacttgat gttgacgttg taggcgccgg gcagctgcac gggcttcttg
      121 gccttgtagg tggtcttgac ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc
      181 tgcttgatct cgcccttcag ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc
      241 cagcccatgg tcttcttctg cattacgggg ccgtcggagg ggaagttggt gccgcgcagc
      301 ttcaccttgt agatgaactc gccgtcctgc agggaggagt cctgggtcac ggtcaccacg
      361 ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc
      421 aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct tggagccgta catgaactga
      481 ggggacagga tgtcccaggc gaagggcagg gggccaccct tggtcacctt cagcttggcg
      541 gtctgggtgc cctcgtaggg gcggccctga ggaaaaaaaa ggaaagggca tgctcgccat
      601 gtgcaagata cagtgttgaa tgtggtgcaa aatataaaga tgtggaagaa aaagccactg
      661 aaaataatat gaggaaataa aaaactagag taaattatca gtggaacaat ttctattata
      721 caagacacta cacccataca ttctcctaat gctcatgtaa gaatttctcc aaaaatacct
      781 tccagcacta caaactagaa aaaaaaagag acatggacac caaattaagt tcttaatagt
      841 gaagaacaaa agaactacct tgcctgctcc agtggatcca gcaaccgcca acaactgtcc
      901 tctttctatc ttgaaattaa tatctttcag gacaggagta ccaagaagtg agaaattact
      961 gaagaagagg ctgtcatcac cattagaagt ttttctattg ttattgtttt gttttgcttt
     1021 ctcaaattat tccccaaatc cctgttaaaa aaaaacacac acacacacac acacacacac
     1081 atcaaaaata aaagatgagt ttgtcagata ttttcaatag atgcttgttt tatgtaccat
     1141 actcacctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg
     1201 caccttgaag cgcatgaact ccttgatgat ggccatgtta tcctcctcgc ccttgctggt
     1261 tccggtgcct ccactttcat cttcaccttt agtgataccg gctgccgtca cgaactcctt
     1321 caggaccatg tgatcgcgct tctcgttcgg gtctttgctc agcgtggtct gggtgctcag
     1381 atagtggttg tccggcaggc gcaccgggcc gtcgccgatc ggggtgttct gctgtttgtg
     1441 gtcggcttcc tgctggctgc cgtcctcaac gttgtggcgg atcttgaagt tagccttgat
     1501 gccgttcttc tgcttgtcgg cttcgatacg gacgttgtgg ctgttgtggt tgtactccag
     1561 cttgtgaccc aggatgttgc cgtcctcctt gaagtcgttg cccttcagct cgatgcggtt
     1621 caccagggtg tcgccctcga acttcacctc cgcgcgggtc ttgtaggtgc cgtcgtcctt
     1681 gaactcgatg gtgcgctcct ggacgtagcc ttccggcatg gcggacttga agaagtcgtg
     1741 acgcttcatg tggtccgggt agcggctgaa gcactgcacg ccgtaggtca gggtggtcac
     1801 gagggtcggc cacggcaccg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt
     1861 gccctcggtg gcatcgccct cgccctcgcc acgcacgctg aacttgtggc cgtttacgtc
     1921 gccgtccagc tcgactttga taggcacagt acctgtaaat agttcctctc ctttactggt
     1981 accatcatca gggaatgagc ttacatttct cctcttcagc atctggtcat ccttcttagc
     2041 tttcctcagc tccacattga cctctattct gcgacgcctc atttctgtac tgtcttttcc
     2101 cttgttcttg aatctgtgaa gacgggcagc tggtgtatta gcattctcgt tggtggatcc
     2161 catggtggca ccggtgaatt ctccaggcga tctgacggtt cactaaacga gctctgctta
     2221 tataggcctc ccaccgtaca cgccacctcg acatactcga gtagttatta atagtaatca
     2281 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     2341 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     2401 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     2461 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     2521 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     2581 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     2641 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     2701 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     2761 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     2821 agcagagctg gtttagtgaa ccgtcagatc cgctagggat cctctagtca gctgacgcgt
     2881 taccaccggt gccaccatgg gaagcgagct gattaaggag aacatgcaca tgaagctgta
     2941 catggagggc accgtggaca accatcactt caagtgcaca tccgagggcg aaggcaagcc
     3001 ctacgagggc acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc
     3061 cttcgacatc ctggctacta gcttcctcta cggcagcaag accttcatca accacaccca
     3121 gggcatcccc gacttcttca agcagtcctt ccctgagggc ttcacatggg agagagtcac
     3181 cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg
     3241 cctcatctac aacgtcaaga tcagaggggt gaacttcaca tccaacggcc ctgtgatgca
     3301 gaagaaaaca ctcggctggg aggccttcac cgagacgctg taccccgctg acggcggcct
     3361 ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg agccatctga tcgcaaacgc
     3421 caagaccaca tatagatcca agaaacccgc taagaacctc aagatgcctg gcgtctacta
     3481 tgtggactac agactggaaa gaatcaagga ggccaacaac gagacctacg tcgagcagca
     3541 cgaggtggca gtggccagat actgcgacct ccctagcaaa ctggggcaca agcttaattc
     3601 cggactcaga tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg
     3661 atccaccgga tctagaggag gaagcggagc tactaacttc agcctgctga agcaggctgg
     3721 agacgtggag gagaaccctg gacctatgtc tgaatatatt cgggtaaccg aagatgagaa
     3781 cgatgagccc attgaaatac catcggaaga cgatgggacg gtgctgctct ccacggttac
     3841 agcccagttt ccaggggcgt gtgggcttcg ctacaggaat ccagtgtctc agtgtatgag
     3901 aggtgtccgg ctggtagaag gaattctgca tgccccagat gctggctggg gaaatctggt
     3961 gtatgttgtc aactatccaa aagataacaa aagaaaaatg gatgagacag atgcttcatc
     4021 agcagtgaaa gtgaaaagag cagtccagaa aacatccgat ttaatagtgt tgggtctccc
     4081 atggaaaaca accgaacagg acctgaaaga gtattttagt acctttggag aagttcttat
     4141 ggtgcaggtc aagaaagatc ttaagactgg tcattcaaag gggtttggct ttgttcgttt
     4201 tacggaatat gaaacacaag tgaaagtaat gtcacagcga catatgatag atggacgatg
     4261 gtgtgactgc aaacttccta attctaagca aagccaagat gagcctttga gaagcagaaa
     4321 agtgtttgtg gggcgctgta cagaggacat gactgaggat gagctgcggg agttcttctc
     4381 tcagtacggg gatgtgatgg atgtcttcat ccccaagcca ttcagggcct ttgcctttgt
     4441 tacatttgca gatgatcaga ttgcgcagtc tctttgtgga gaggacttga tcattaaagg
     4501 aatcagcgtt catatatcca atgccgaacc taagcacaat agcaatagac agttagaaag
     4561 aagtggaaga tttggtggta atccaggtgg ctttgggaat cagggtggat ttggtaatag
     4621 cagagggggt ggagctggtt tgggaaacaa tcaaggtagt aatatgggtt ttgggatgaa
     4681 ctttggtgcg ttcagcatta atccagccat gatggctgcc gcccaggcag cactacagag
     4741 cagttggggt atgatgggca tgttagccag ccagcagaac cagtcaggcc catcgggtaa
     4801 taaccaaaac caaggcaaca tgcagaggga gccaaaccag gccttcggtt ctggaaataa
     4861 ctcttatagt ggctctaatt ctggtgcagc aattggttgg ggatcagcat ccaatgcagg
     4921 gtcgggcagt ggttttaatg gaggctttgg ctcaagcatg gattctaagt cttctggctg
     4981 gggaatgtag actagaccca gctttcttgt acaaagtggt tgatcctcta gactgagaac
     5041 ttcagggtga gtttggggac ccttgattgt tctttctttt tcgctattgt aaaattcatg
     5101 ttatatggag ggggcaaagt tttcagggtg ttgtttagaa tgggaagatg tcccttgtat
     5161 caccatggac cctcatgata attttgtttc tttcactttc tactctgttg acaaccattg
     5221 tctcctctta ttttcttttc attttctgta actttttcgt taaactttag cttgcatttg
     5281 taacgaattt ttaaattcac ttttgtttat ttgtcagatt gtaagtactt tctctaatca
     5341 cttttttttc aaggcaatca gggtatatta tattgtactt cagcacagtt ttagagaaca
     5401 attgttataa ttaaatgata aggtagaata tttctgcata taaattctgg ctggcgtgga
     5461 aatattctta ttggtagaaa caactacacc ctggtcatca tcctgccttt ctctttatgg
     5521 ttacaatgat atacactgtt tgagatgagg ataaaatact ctgagtccaa accgggcccc
     5581 tctgctaacc atgttcatgc cttcttctct ttcctacagc tcctgggcaa cgtgctggtt
     5641 gttgtgctgt ctcatcattt tggcaaagaa ttcactcctc aggtgcaggc tgcctatcag
     5701 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     5761 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     5821 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     5881 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     5941 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     6001 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     6061 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     6121 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     6181 cttctcttat ggagatccct cgactgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6241 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6301 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6361 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6421 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6481 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6541 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6601 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6661 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6721 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6781 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6841 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     6901 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6961 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7021 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7081 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7141 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7201 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7261 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7321 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7381 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7441 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7501 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7561 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7621 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7681 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     7741 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     7801 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     7861 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     7921 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     7981 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8041 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8101 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8161 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     8221 taacctataa aaataggcgt atcacgaggc cctttcgtct tcactcgacg cggccagctt
     8281 ggtcgagctg gatacttccc gtccgccagg gggacatgcc ggcgatgctg aaggtcgcgc
     8341 gcattcccga tgaagaggcc ggttaccgcc tgttgacctg gtgggacggg cagggcgc
//
