LOCUS       sequence-208305-        8414 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To test the effect of sequence on TDP43 splicing activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8414)
  TITLE     Rajat Rohatgi TDP43 plasmids
REFERENCE   2  (bases 1 to 8414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHBS1338 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G3...
FEATURES             Location/Qualifiers
     source          1..8414
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(272..291)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     541..559
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2181..2190
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        2343..2646
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2647..2850
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2801..2821
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2912..2921
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2918..3619
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     misc_feature    3620..3685
                     /label=MCS
                     /note="multiple cloning site"
     CDS             3710..3766
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(5012..5036)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          5063..5635
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(5767..5786)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5832..5887
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5886..5905)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6285..6302)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6456..7044)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6536..6555)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7215..8006,8007..8075))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7838..7857
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8076..8180)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8248..8266
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tacagctcgt ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta ctgttccacg
       61 atggtgtagt cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt gtaggcgccg
      121 ggcagctgca cgggcttctt ggccttgtag gtggtcttga cctcagcgtc gtagtggccg
      181 ccgtccttca gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac
      241 atccgctcgg aggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcggag
      301 gggaagttgg tgccgcgcag cttcaccttg tagatgaact cgccgtcctg cagggaggag
      361 tcctgggtca cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag
      421 ccctcgggga aggacagctt caagtagtcg gggatgtcgg cggggtgctt cacgtaggcc
      481 ttggagccgt acatgaactg aggggacagg atgtcccagg cgaagggcag ggggccaccc
      541 ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg ggcggccctg aggaaaaaaa
      601 aggaaagggc atgctcgcca tgtgcaagat acagtgttga atgtggtgca aaatataaag
      661 atgtggaaga aaaagccact gaaaataata tgaggaaata aaaaactaga gtaaattatc
      721 agtggaacaa tttctattat acaagacact acacccatac attctcctaa tgctcatgta
      781 agaatttctc caaaaatacc ttccagcact acaaactaga aaaaaaaaga gacatggaca
      841 ccaaattaag ttcttaatag tgaagaacaa aagaactacc ttgcctgctc cagtggatcc
      901 agcaaccgcc aacaactgtc ctctttctat cttgaaatta atatctttca ggacaggagt
      961 accaagaagt gagaaattac tgaagaagag gctgtcatca ccattagaag tttttctatt
     1021 gttattgttt tgttttgctt tctcaaatta ttccccaaat ccctgttaaa aaaaaacaca
     1081 cacacacaca cacacacaca catcaaaaat aaaagatgag tttgtcagat attttcaata
     1141 gatgcttgtt ttatgtacca tactcacctc gccctcgccc tcgatctcga actcgtggcc
     1201 gttcacggag ccctccatgt gcaccttgaa gcgcatgaac tccttgatga tggccatgtt
     1261 atcctcctcg cccttgctgg ttccggtgcc tccactttca tcttcacctt tagtgatacc
     1321 ggctgccgtc acgaactcct tcaggaccat gtgatcgcgc ttctcgttcg ggtctttgct
     1381 cagcgtggtc tgggtgctca gatagtggtt gtccggcagg cgcaccgggc cgtcgccgat
     1441 cggggtgttc tgctgtttgt ggtcggcttc ctgctggctg ccgtcctcaa cgttgtggcg
     1501 gatcttgaag ttagccttga tgccgttctt ctgcttgtcg gcttcgatac ggacgttgtg
     1561 gctgttgtgg ttgtactcca gcttgtgacc caggatgttg ccgtcctcct tgaagtcgtt
     1621 gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct ccgcgcgggt
     1681 cttgtaggtg ccgtcgtcct tgaactcgat ggtgcgctcc tggacgtagc cttccggcat
     1741 ggcggacttg aagaagtcgt gacgcttcat gtggtccggg tagcggctga agcactgcac
     1801 gccgtaggtc agggtggtca cgagggtcgg ccacggcacc ggcagcttgc cggtggtgca
     1861 gatgaacttc agggtcagct tgccctcggt ggcatcgccc tcgccctcgc cacgcacgct
     1921 gaacttgtgg ccgtttacgt cgccgtccag ctcgactttg ataggcacag tacctgtaaa
     1981 tagttcctct cctttactgg taccatcatc agggaatgag cttacatttc tcctcttcag
     2041 catctggtca tccttcttag ctttcctcag ctccacattg acctctattc tgcgacgcct
     2101 catttctgta ctgtcttttc ccttgttctt gaatctgtga agacgggcag ctggtgtatt
     2161 agcattctcg ttggtggatc ccatggtggc accggtgaat tctccaggcg atctgacggt
     2221 tcactaaacg agctctgctt atataggcct cccaccgtac acgccacctc gacatactcg
     2281 agtagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     2341 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     2401 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     2461 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     2521 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     2581 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     2641 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     2701 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     2761 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     2821 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctaggga
     2881 tcctctagtc agctgacgcg ttaccaccgg tgccaccatg ggaagcgagc tgattaagga
     2941 gaacatgcac atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac
     3001 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga
     3061 gggcggccct ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa
     3121 gaccttcatc aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg
     3181 cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga
     3241 caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac
     3301 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct
     3361 gtaccccgct gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg
     3421 gagccatctg atcgcaaacg ccaagaccac atatagatcc aagaaacccg ctaagaacct
     3481 caagatgcct ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa
     3541 cgagacctac gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa
     3601 actggggcac aagcttaatt ccggactcag atctcgagct caagcttcga attctgcagt
     3661 cgacggtacc gcgggcccgg gatccaccgg atctagagga ggaagcggag ctactaactt
     3721 cagcctgctg aagcaggctg gagacgtgga ggagaaccct ggacctatgt ctgaatatat
     3781 tcgggtaacc gaagatgaga acgatgagcc cattgaaata ccatcggaag acgatgggac
     3841 ggtgctgctc tccacggtta cagcccagtt tccaggggcg tgtgggcttc gctacaggaa
     3901 tccagtgtct cagtgtatga gaggtgtccg gctggtagaa ggaattctgc atgccccaga
     3961 tgctggctgg ggaaatctgg tgtatgttgt caactatcca aaagataaca aaagaaaaat
     4021 ggatgagaca gatgcttcat cagcagtgaa agtgaaaaga gcagtccaga aaacatccga
     4081 tttaatagtg ttgggtctcc catggaaaac aaccgaacag gacctgaaag agtattttag
     4141 tacctttgga gaagttctta tggtgcaggt caagaaagat cttaagactg gtcattcaaa
     4201 ggggtttggc tttgttcgtt ttacggaata tgaaacacaa gtgaaagtaa tgtcacagcg
     4261 acatatgata gatggacgat ggtgtgactg caaacttcct aattctaagc aaagccaaga
     4321 tgagcctttg agaagcagaa aagtgtttgt ggggcgctgt acagaggaca tgactgagga
     4381 tgagctgcgg gagttcttct ctcagtacgg ggatgtgatg gatgtcttca tccccaagcc
     4441 attcagggcc tttgcctttg ttacatttgc agatgatcag attgcgcagt ctctttgtgg
     4501 agaggacttg atcattaaag gaatcagcgt tcatatatcc aatgccgaac ctaagcacaa
     4561 tagcaataga cagttagaaa gaagtggaag atttggtggt aatccaggtg gctttgggaa
     4621 tcagggtgga tttggtaata gcagaggggg tggagctggt ttgggaaaca atcaaggtag
     4681 taatatgggt ggtgggatga actttggtgc gttcagcatt aatccagcca tgatggctgc
     4741 cgcccaggca gcactacaga gcagttgggg tatgatgggc atgttagcca gccagcagaa
     4801 ccagtcagtc ccatcgggta ataaccaaaa ccaaggcaac atgcagaggg agccaaacca
     4861 ggccttcggt tctggaaata actcttatag tggctctaat tctggtgcag caattggttg
     4921 gggatcagca tccaatgcag ggtcgggcag tggttttaat ggaggctttg gctcaagcat
     4981 ggattctaag tcttctggct ggggaatgta gacccagctt tcttgtacaa agtggttgat
     5041 cctctagact gagaacttca gggtgagttt ggggaccctt gattgttctt tctttttcgc
     5101 tattgtaaaa ttcatgttat atggaggggg caaagttttc agggtgttgt ttagaatggg
     5161 aagatgtccc ttgtatcacc atggaccctc atgataattt tgtttctttc actttctact
     5221 ctgttgacaa ccattgtctc ctcttatttt cttttcattt tctgtaactt tttcgttaaa
     5281 ctttagcttg catttgtaac gaatttttaa attcactttt gtttatttgt cagattgtaa
     5341 gtactttctc taatcacttt tttttcaagg caatcagggt atattatatt gtacttcagc
     5401 acagttttag agaacaattg ttataattaa atgataaggt agaatatttc tgcatataaa
     5461 ttctggctgg cgtggaaata ttcttattgg tagaaacaac tacaccctgg tcatcatcct
     5521 gcctttctct ttatggttac aatgatatac actgtttgag atgaggataa aatactctga
     5581 gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc ttctctttcc tacagctcct
     5641 gggcaacgtg ctggttgttg tgctgtctca tcattttggc aaagaattca ctcctcaggt
     5701 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca
     5761 ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct
     5821 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     5881 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
     5941 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata
     6001 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc
     6061 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc
     6121 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc
     6181 agtcatagct gtccctcttc tcttatggag atccctcgac tgcattaatg aatcggccaa
     6241 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6301 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6361 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6421 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6481 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6541 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6601 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6661 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6721 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6781 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6841 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     6901 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6961 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7021 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7081 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7141 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7201 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7261 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7321 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7381 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7441 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7501 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7561 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7621 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7681 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7741 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7801 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     7861 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     7921 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     7981 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8041 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8101 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8161 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     8221 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcac
     8281 tcgacgcggc cagcttggtc gagctggata cttcccgtcc gccaggggga catgccggcg
     8341 atgctgaagg tcgcgcgcat tcccgatgaa gaggccggtt accgcctgtt gacctggtgg
     8401 gacgggcagg gcgc
//
