LOCUS       sequence-208309-        5779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To quickly insert CTD mutants into TDP43 splicing reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5779)
  TITLE     Rajat Rohatgi TDP43 plasmids
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHBS1178 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A...
FEATURES             Location/Qualifiers
     source          1..5779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(234..253)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     503..521
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2143..2152
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        2305..2608
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2609..2812
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2763..2783
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2874..2883
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2880..3581
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     misc_feature    3582..3647
                     /label=MCS
                     /note="multiple cloning site"
     CDS             3672..3728
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(4524..4548)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          4603..5175
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(5307..5326)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5372..5427
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5426..5445)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccaactt
       61 gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc ttggccttgt aggtggtctt
      121 gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga tctcgccctt
      181 cagggcgccg tcctcggggt acatccgctc ggaggaggcc tcccagccca tggtcttctt
      241 ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct tgtagatgaa
      301 ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt
      361 catcacgcgc tcccacttga agccctcggg gaaggacagc ttcaagtagt cggggatgtc
      421 ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac tgaggggaca ggatgtccca
      481 ggcgaagggc agggggccac ccttggtcac cttcagcttg gcggtctggg tgccctcgta
      541 ggggcggccc tgaggaaaaa aaaggaaagg gcatgctcgc catgtgcaag atacagtgtt
      601 gaatgtggtg caaaatataa agatgtggaa gaaaaagcca ctgaaaataa tatgaggaaa
      661 taaaaaacta gagtaaatta tcagtggaac aatttctatt atacaagaca ctacacccat
      721 acattctcct aatgctcatg taagaatttc tccaaaaata ccttccagca ctacaaacta
      781 gaaaaaaaaa gagacatgga caccaaatta agttcttaat agtgaagaac aaaagaacta
      841 ccttgcctgc tccagtggat ccagcaaccg ccaacaactg tcctctttct atcttgaaat
      901 taatatcttt caggacagga gtaccaagaa gtgagaaatt actgaagaag aggctgtcat
      961 caccattaga agtttttcta ttgttattgt tttgttttgc tttctcaaat tattccccaa
     1021 atccctgtta aaaaaaaaca cacacacaca cacacacaca cacatcaaaa ataaaagatg
     1081 agtttgtcag atattttcaa tagatgcttg ttttatgtac catactcacc tcgccctcgc
     1141 cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg aagcgcatga
     1201 actccttgat gatggccatg ttatcctcct cgcccttgct ggttccggtg cctccacttt
     1261 catcttcacc tttagtgata ccggctgccg tcacgaactc cttcaggacc atgtgatcgc
     1321 gcttctcgtt cgggtctttg ctcagcgtgg tctgggtgct cagatagtgg ttgtccggca
     1381 ggcgcaccgg gccgtcgccg atcggggtgt tctgctgttt gtggtcggct tcctgctggc
     1441 tgccgtcctc aacgttgtgg cggatcttga agttagcctt gatgccgttc ttctgcttgt
     1501 cggcttcgat acggacgttg tggctgttgt ggttgtactc cagcttgtga cccaggatgt
     1561 tgccgtcctc cttgaagtcg ttgcccttca gctcgatgcg gttcaccagg gtgtcgccct
     1621 cgaacttcac ctccgcgcgg gtcttgtagg tgccgtcgtc cttgaactcg atggtgcgct
     1681 cctggacgta gccttccggc atggcggact tgaagaagtc gtgacgcttc atgtggtccg
     1741 ggtagcggct gaagcactgc acgccgtagg tcagggtggt cacgagggtc ggccacggca
     1801 ccggcagctt gccggtggtg cagatgaact tcagggtcag cttgccctcg gtggcatcgc
     1861 cctcgccctc gccacgcacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgactt
     1921 tgataggcac agtacctgta aatagttcct ctcctttact ggtaccatca tcagggaatg
     1981 agcttacatt tctcctcttc agcatctggt catccttctt agctttcctc agctccacat
     2041 tgacctctat tctgcgacgc ctcatttctg tactgtcttt tcccttgttc ttgaatctgt
     2101 gaagacgggc agctggtgta ttagcattct cgttggtgga tcccatggtg gcaccggtga
     2161 attctccagg cgatctgacg gttcactaaa cgagctctgc ttatataggc ctcccaccgt
     2221 acacgccacc tcgacatact cgagtagtta ttaatagtaa tcaattacgg ggtcattagt
     2281 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     2341 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     2401 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     2461 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     2521 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     2581 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     2641 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     2701 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     2761 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
     2821 gaaccgtcag atccgctagg gatcctctag tcagctgacg cgttaccacc ggtgccacca
     2881 tgggaagcga gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg
     2941 acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
     3001 ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
     3061 ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct
     3121 tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg
     3181 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca
     3241 agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct
     3301 gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca
     3361 tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa cgccaagacc acatatagat
     3421 ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg
     3481 aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca
     3541 gatactgcga cctccctagc aaactggggc acaagcttaa ttccggactc agatctcgag
     3601 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc ggatctagag
     3661 gaggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc
     3721 ctggacctat gtctgaatat attcgggtaa ccgaagatga gaacgatgag cccattgaaa
     3781 taccatcgga agacgatggg acggtgctgc tctccacggt tacagcccag tttccagggg
     3841 cgtgtgggct tcgctacagg aatccagtgt ctcagtgtat gagaggtgtc cggctggtag
     3901 aaggaattct gcatgcccca gatgctggct ggggaaatct ggtgtatgtt gtcaactatc
     3961 caaaagataa caaaagaaaa atggatgaga cagatgcttc atcagcagtg aaagtgaaaa
     4021 gagcagtcca gaaaacatcc gatttaatag tgttgggtct cccatggaaa acaaccgaac
     4081 aggacctgaa agagtatttt agtacctttg gagaagttct tatggtgcag gtcaagaaag
     4141 atcttaagac tggtcattca aaggggtttg gctttgttcg ttttacggaa tatgaaacac
     4201 aagtgaaagt aatgtcacag cgacatatga tagatggacg atggtgtgac tgcaaacttc
     4261 ctaattctaa gcaaagccaa gatgagcctt tgagaagcag aaaagtgttt gtggggcgct
     4321 gtacagagga catgactgag gatgagctgc gggagttctt ctctcagtac ggggatgtga
     4381 tggatgtctt catccccaag ccattcaggg cctttgcctt tgttacattt gcagatgatc
     4441 agattgcgca gtctctttgt ggagaggact tgatcattaa aggaatcagc gttcatatat
     4501 ccaatgccga acctgatatc tagacccagc tttcttgtac aaagtggttg atcctctact
     4561 agtaagcttg catgcgatat ctctagagct gagaacttca gggtgagttt ggggaccctt
     4621 gattgttctt tctttttcgc tattgtaaaa ttcatgttat atggaggggg caaagttttc
     4681 agggtgttgt ttagaatggg aagatgtccc ttgtatcacc atggaccctc atgataattt
     4741 tgtttctttc actttctact ctgttgacaa ccattgtctc ctcttatttt cttttcattt
     4801 tctgtaactt tttcgttaaa ctttagcttg catttgtaac gaatttttaa attcactttt
     4861 gtttatttgt cagattgtaa gtactttctc taatcacttt tttttcaagg caatcagggt
     4921 atattatatt gtacttcagc acagttttag agaacaattg ttataattaa atgataaggt
     4981 agaatatttc tgcatataaa ttctggctgg cgtggaaata ttcttattgg tagaaacaac
     5041 tacaccctgg tcatcatcct gcctttctct ttatggttac aatgatatac actgtttgag
     5101 atgaggataa aatactctga gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc
     5161 ttctctttcc tacagctcct gggcaacgtg ctggttgttg tgctgtctca tcattttggc
     5221 aaagaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat
     5281 gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc
     5341 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
     5401 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
     5461 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc
     5521 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
     5581 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
     5641 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
     5701 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatgaac tcggcgccct
     5761 gcccgtccca ccaggtcaa
//
