LOCUS       sequence-208323-       13240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A tyrosine kinase biosensor based on fluorescence resonance energy 
            transfer (FRET) and those based on bioluminescence energy transfer 
            (BRET).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13240)
  AUTHORS   Komatsu N, Terai K, Imanishi A, Kamioka Y, Sumiyama K, Jin T, Okada 
            Y, Nagai T, Matsuda M
  TITLE     A platform of BRET-FRET hybrid biosensors for optogenetics, chemical
            screening, and in vivo imaging.
  JOURNAL   Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.1038/s41598-018-27174-x.
  PUBMED    29895862
REFERENCE   2  (bases 1 to 13240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-06-12"; volume: "8(1):8984. doi"; pages: " 
            10.1038/s41598-018-27174-x"
COMMENT     Sequence Label: pCSIIbsr-6085x
FEATURES             Location/Qualifiers
     source          1..13240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(52..71)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        243..622
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        624..822
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     777..797
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             840..1020
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1067..1192
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2102..2146
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2420..2537
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2593..3755
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2824..3746
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3703..3723
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3790..4500
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELY"
     primer_bind     complement(5266..5287)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5527..5546)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    join(6914..7488,7489..7491,7492..7500)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7081..7098)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7308..7327
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7501..7899
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7943..8531
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7996..8016)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8414..8425)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9050..9230
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9256..9273)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9262..9486
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9532..9960
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9619..9638)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9829..9850
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9969..9989)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9974..10303
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10154..10289
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10216..10235
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10351..10398
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10417..10791
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10921..11042
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10958..10977)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11012..11031
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11091..11107)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11091..11107)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11104..11126)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11115..11131
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11139..11145,11146..11163,11164..11169))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11184..11205
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11322..11339)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11493..12081)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11573..11592)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12252..13043,13044..13112))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12875..12894
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13113..13217)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
       61 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
      121 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
      181 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
      241 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
      301 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      361 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      421 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      481 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      541 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      601 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      661 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      721 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      781 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg
      841 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
      901 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
      961 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     1021 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     1081 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     1141 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     1201 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     1261 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     1321 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     1381 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     1441 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     1501 aaacaaaagt aagaccaccg cacagcaagg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctacaaa
     2401 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     2461 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     2521 caaaaattca aaattttcgg gtttattaca gggacagcag aaattcactt tgaattaatt
     2581 caagcttcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     2641 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
     2701 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     2761 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     2821 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
     2881 gtgccttgaa ttacttccac ctggctccag tacgtgattc ttgatcccga gctggagcca
     2941 ggggcgggcc ttgcgcttta ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
     3001 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
     3061 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
     3121 atagtcttgt aaatgcgggc caggatctgc acactggtat ttcggttttt ggggccgcgg
     3181 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
     3241 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
     3301 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
     3361 cgtgagcgga aagatggccg cttcccggcc ctgctccagg gggctcaaaa tggaggacgc
     3421 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaggggcc tttccgtcct
     3481 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
     3541 tctggagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     3601 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     3661 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     3721 ttcaaagttt ttttcttcca tttcaggtgt cgtgaacacg ctaccggtct cgacgaattc
     3781 gcccttagca tgtctaaagg tgaagaatta ttcactggtg ttgtcccaat tttggttgaa
     3841 ttagatggtg atgttaatgg tcacaaattt tctgtctccg gtgaaggtga aggtgatgct
     3901 acgtacggta aattgacctt aaaattactc tgtactactg gtaaattgcc agttccatgg
     3961 ccaaccttag tcactacttt aggttatggt gttcaatgtt ttgctagata cccagatcat
     4021 atgaaacaac atgacttttt caagtctgcc atgccagaag gttatgttca agaaagaact
     4081 atttttttca aagatgacgg taactacaag accagagctg aagtcaagtt tgaaggtgat
     4141 accttagtta atagaatcga attaaaaggt attgatttta aagaagatgg taacatttta
     4201 ggtcacaaat tggaatacaa ctataactct cacaatgttt acatcactgc tgacaaacaa
     4261 aagaatggta tcaaagctaa cttcaaaatt agacacaaca ttgaagatgg tggtgttcaa
     4321 ttagctgacc attatcaaca aaatactcca attggtgatg gtccagtctt gttaccagac
     4381 aaccattact tatcctatca atctgcctta ttcaaagatc caaacgaaaa gagagaccac
     4441 atggtcttgt tagaattttt gactgctgct ggtattaccg agggtatgaa tgaattgtac
     4501 ctcgacatgg cgggcaactt cgactcggag gagcggagta gctggtactg gggccgcttg
     4561 agccggcagg aggcggtggc gctattgcag ggccagcggc acggggtttt cctggtgcgg
     4621 gactcgagca ccagccccgg ggactatgtg ctcagcgtct ccgaaaactc gcgcgtctcc
     4681 cactacatca tcaacagcag cggcccgcgc cctccagtgc ctccgtcgcc cgctcagcct
     4741 ccgccgggag tgagtccctc caggctccga ataggagatc aagaatttga ttcattgcct
     4801 gctttactgg aattctacaa aatacactat ttggacacta caacactgat agaaccagtt
     4861 tccagatcaa ggcagggtag tggagtgatt ctcaggcagg aggaggcaga gtatgtgcgg
     4921 gccctctttg actttaatgg gaatgatgaa gaagatcttc cctttaagaa aggagacatc
     4981 ctgagaatcc gggataagcc tgaagagcag tggtggaatg cagaggacag cgaaggaaag
     5041 agggggatga ttcctgtccc ttacgtggag aagtatagac ccgcctccgc ctcagtatcg
     5101 gctctgattg gaggtaacca ggagggttcc cacccacagc cactgggtgg gccggagcct
     5161 gggccctatg cccaacccag cgtcaacact ccgctcccta acctccagaa tggcggccgc
     5221 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     5281 ggcgacgtca acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     5341 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     5401 ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag
     5461 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     5521 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     5581 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     5641 aagctggagt acaactactt cagcgggaac gtctatatca ccgccgacaa gcagaagaac
     5701 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc
     5761 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     5821 tacctgagca cccagtccgc cttaagcaaa gaccccaacg agaagcgcga tcacatggtc
     5881 ctgctggagt tcttgaccgc cgccgggggt accaaggtgt acgaccccga gcagaggaag
     5941 aggatgatca ccggccccca gtggtgggcc aggtgcaagc agatgaacgt gctggacagc
     6001 ttcatcaact actacgacag cgagaagcac gccgagaacg ccgtgatctt cctgcacggc
     6061 aacgccacta gcagctacct gtggaggcac gtggtgcccc acatcgagcc cgtggccagg
     6121 tgcatcatcc ccgatctgat cggcatgggc aagagcggca agagcggcaa cggcagctac
     6181 aggctgctgg accactacaa gtacctgacc gcctggttcg agcttctgaa cctgcccaag
     6241 aagatcatct tcgtgggcca cgactggggc gccgccctgg ccttccacta cgcctacgag
     6301 caccaggaca ggatcaaggc catcgtgcac atggagagcg tggtggacgt gatcgagagc
     6361 tgggacgagt ggccagacat cgaggaggac atcgccctga tcaagagcga ggagggcgag
     6421 aagatggtgc tggagaacaa cttcttcgtg gagaccgtgc tgcccagcaa gatcatgaga
     6481 aagctggagc ccgaggagtt cgccgcctac ctggagccct tcaaggagaa gggcgaggtg
     6541 agaagaccca ccctgagctg gcccagagag atccccctgg tgaagggcgg caagcccgac
     6601 gtggtgcaga tcgtgagaaa ctacaacgcc tacctgagag ccagcgacga cctgcccaag
     6661 ctgttcatcg agggcgaccc cggcttcttc agcaacgcca tcgtggaggg cgccaagaag
     6721 ttccccaaca ccgagttcgt gaaggtgaag ggcctgcact tcctccagga ggacgccccc
     6781 gacgagatgg gcaagtacat caagagcttc gtggagagag tgctgaagaa cgagcagtct
     6841 agaaagatga gcaaagatgg taaaaagaag aaaaagaagt caaagacaaa gtgtgtaatt
     6901 atgtaaggat ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg
     6961 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     7021 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     7081 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     7141 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     7201 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     7261 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     7321 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     7381 tggggcctcg gtacacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     7441 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacaacc
     7501 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
     7561 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc
     7621 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
     7681 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
     7741 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
     7801 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
     7861 cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag tcgagcctct agagttaaca
     7921 tcgagggatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     7981 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     8041 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     8101 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     8161 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     8221 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     8281 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     8341 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     8401 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     8461 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     8521 cgcctccccg catcgatacc gtcgagacct agaaaaacat ggagcaatca caagtagcaa
     8581 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     8641 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     8701 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     8761 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta
     8821 cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc
     8881 agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc
     8941 tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag
     9001 ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg
     9061 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     9121 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     9181 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt
     9241 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     9301 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     9361 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     9421 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     9481 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     9541 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     9601 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     9661 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     9721 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     9781 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     9841 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     9901 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     9961 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
    10021 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
    10081 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
    10141 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
    10201 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
    10261 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
    10321 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
    10381 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
    10441 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
    10501 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
    10561 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
    10621 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
    10681 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
    10741 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
    10801 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
    10861 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
    10921 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    10981 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    11041 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
    11101 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
    11161 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
    11221 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
    11281 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    11341 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    11401 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    11461 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    11521 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    11581 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    11641 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    11701 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    11761 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    11821 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    11881 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
    11941 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    12001 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    12061 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    12121 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    12181 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    12241 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    12301 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    12361 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    12421 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    12481 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    12541 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    12601 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    12661 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    12721 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    12781 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    12841 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    12901 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    12961 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    13021 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    13081 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    13141 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    13201 caaatagggg ttccgcgcac atttccccga aaagtgccac
//
