LOCUS       sequence-208332-         596 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial protein of E.coli DHFR C85A/C152S mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Liu CT, Layfield JP, Stewart RJ 3rd, French JB, Hanoian P, Asbury 
            JB, Hammes-Schiffer S, Benkovic SJ
  TITLE     Probing the electrostatics of active site microenvironments along 
            the catalytic cycle for Escherichia coli dihydrofolate reductase.
  JOURNAL   J Am Chem Soc. 2014 Jul 23;136(29):10349-60. doi: 10.1021/ja5038947.
            Epub 2014 Jul 11.
  PUBMED    24977791
REFERENCE   2  (bases 1 to 596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja5038947";
            journalName: "J Am Chem Soc"; date: "2014-07-23- 23"; volume: "136";
            issue: "29"; pages: "10349-60"
COMMENT     Sequence Label: pET22b-ecDHFR-dCys
FEATURES             Location/Qualifiers
     source          1..596
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             557..574
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacat atgatcagtc tgattgcggc gctagcggta
       61 gatcgcgtta tcggcatgga aaacgccatg ccatggaacc tgcctgccga tctcgcctgg
      121 tttaaacgca acaccttaaa taaacccgtg attatggggc gccatacctg ggaatcaatc
      181 ggtaggcctt tgcccggccg caaaaatatt atcctcagca gtcaacccgg gaccgatgat
      241 cgggttacct gggttaaatc ggtcgacgaa gccatcgcgg cggctggtga cgtaccagaa
      301 atcatggtga ttggcggcgg acgcgtttat gaacagttct tgccaaaagc gcaaaaactg
      361 tatctgacgc atatcgatgc agaagtggaa ggcgacaccc attttccgga ttacgagccg
      421 gatgactggg aatcggtatt cagcgaattc cacgatgctg atgcgcagaa ctcgcatagc
      481 tatagtttcg aaatcctcga gcgtcgttaa ggatccgaat tcgagctccg tcgacaagct
      541 tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta acaaag
//
