LOCUS       sequence-208336-         625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of E.coli DHFR C85A/C152S/T46C mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Liu CT, Layfield JP, Stewart RJ 3rd, French JB, Hanoian P, Asbury 
            JB, Hammes-Schiffer S, Benkovic SJ
  TITLE     Probing the electrostatics of active site microenvironments along 
            the catalytic cycle for Escherichia coli dihydrofolate reductase.
  JOURNAL   J Am Chem Soc. 2014 Jul 23;136(29):10349-60. doi: 10.1021/ja5038947.
            Epub 2014 Jul 11.
  PUBMED    24977791
REFERENCE   2  (bases 1 to 625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja5038947";
            journalName: "J Am Chem Soc"; date: "2014-07-23- 23"; volume: "136";
            issue: "29"; pages: "10349-60"
COMMENT     Sequence Label: pET22b-ecDHFR-dCys-T46C
FEATURES             Location/Qualifiers
     source          1..625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             554..571
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtttaacttt aagaaggaga tatacatatg atcagtctga ttgcggcgct agcggtagat
       61 cgcgttatcg gcatggaaaa cgccatgcca tggaacctgc ctgccgatct cgcctggttt
      121 aaacgcaaca ccttaaataa acccgtgatt atggggcgcc attgctggga atcaatcggt
      181 aggcctttgc ccggccgcaa aaatattatc ctcagcagtc aacccgggac cgatgatcgg
      241 gttacctggg ttaaatcggt cgacgaagcc atcgcggcgg ctggtgacgt accagaaatc
      301 atggtgattg gcggcggacg cgtttatgaa cagttcttgc caaaagcgca aaaactgtat
      361 ctgacgcata tcgatgcaga agtggaaggc gacacccatt ttccggatta cgagccggat
      421 gactgggaat cggtattcag cgaattccac gatgctgatg cgcagaactc gcatagctat
      481 agtttcgaaa tcctcgagcg tcgttaagga tccgaattcg agctccgtcg acaagcttgc
      541 ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa
      601 ggaagctgag ttggctgctg ccacc
//
