LOCUS       sequence-208339-        8234 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one dox-repressible expression of cDNA with optional N-term 
            GFP tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8234)
  TITLE     pCW57.1 N-term GFP tTA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCW57.1 N-term GFP tTA
FEATURES             Location/Qualifiers
     source          1..8234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      543..552
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(549..551,552..554,555..1265)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(549..551,552..554,555..1265)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(594..615)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(855..874)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1202..1223
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1311..1331
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     promoter        1371..1881
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1800..1818
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1891..2283
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             2293..2346
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2347..2964,2965..2970,2971..3006,3007..3009,3010..3045,
                     3046..3048,3049..3084,3085..3093)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3133..3153)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3551..3562)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3767..4000
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4072..4193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4109..4128)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4163..4182
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4233..4368
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4368..4439
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4440..4508,4509..5300)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4658..4677)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5471..6059
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5960..5979
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6213..6230
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6347..6368
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6383..6388,6389..6406,6407..6413)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6421..6437
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6426..6448
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6445..6461
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6445..6461
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6480..6500
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6482..6500
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6528..6754
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6755..6935
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6982..7107
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7600..7833
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8018..8062
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      601 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      661 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      721 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
      781 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      841 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      901 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      961 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     1021 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     1081 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     1141 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     1201 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     1261 acaagtccgg aggggatcgg tggagctcca ccggtggcgg ccgctctaga gagaatcttt
     1321 attttcaggg cgccggtgga ggtggagggt cgacgggaga attcggatcc ggggttgggg
     1381 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
     1441 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
     1501 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
     1561 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
     1621 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
     1681 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
     1741 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
     1801 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
     1861 atcaccgacc tctctcccca gcaattcacc atggttcctt tgtctcaaga agaatccacc
     1921 ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc
     1981 gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt
     2041 actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc
     2101 aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga
     2161 cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt
     2221 gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag
     2281 ggccccggtg ccgaaggtag aggttctctc ctcacttgtg gtgatgttga agaaaaccct
     2341 ggtccaatgt ctagactgga caagagcaaa gtcataaact ctgctctgga attactcaat
     2401 gaagtcggta tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag
     2461 cctaccctgt actggcacgt gaagaacaag cgggccctgc tcgatgccct ggcaatcgag
     2521 atgctggaca ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt
     2581 ctgcggaaca acgccaagtc attccgctgt gctctcctct cacatcgcga cggggctaaa
     2641 gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg
     2701 ttcctgtgtc agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc
     2761 cactttacac tgggctgcgt attggaggat caggagcatc aagtagcaaa agaggaaaga
     2821 gagacaccta ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac
     2881 catcagggag ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag
     2941 aaacagctaa agtgcgaaag cggcgggccg gccgacgccc ttgacgattt tgacttagac
     3001 atgctcccag ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt
     3061 gacgattttg accttgacat gctccccggg taactaagta aggatcgatc caagatatcg
     3121 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     3181 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     3241 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     3301 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     3361 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     3421 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     3481 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     3541 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     3601 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     3661 ctccccgcct gtttcgcctc ggcgtccgga ctagaggtac ctttaagacc aatgacttac
     3721 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     3781 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     3841 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     3901 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     3961 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     4021 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     4081 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     4141 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     4201 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4261 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4321 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggcctt tcaaatatgt
     4381 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     4441 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     4501 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     4561 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     4621 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     4681 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4741 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     4801 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     4861 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     4921 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     4981 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     5041 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     5101 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     5161 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     5221 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     5281 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     5341 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5401 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5461 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5521 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5581 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5641 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5701 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5761 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5821 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5881 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5941 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6001 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6061 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6121 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     6181 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     6241 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     6301 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     6361 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     6421 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     6481 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     6541 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     6601 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     6661 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     6721 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     6781 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6841 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6901 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     6961 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     7021 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     7081 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     7141 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     7201 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     7261 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     7321 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     7381 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     7441 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     7501 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     7561 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     7621 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     7681 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     7741 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     7801 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     7861 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     7921 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     7981 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     8041 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     8101 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     8161 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     8221 aaccccgagg ggac
//
