LOCUS       sequence-208357-        3504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CYP101A1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Iwata F, Hirakawa H, Nagamune T
  TITLE     A Stable Artificial Multienzymatic Complex Using a Heterotrimeric 
            Protein From Metallosphaera sedula.
  JOURNAL   Biotechnol J. 2018 Nov;13(11):e1700662. doi: 10.1002/biot.201700662.
            Epub 2018 Apr 30.
  PUBMED    29663675
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J."; date: "2018-11"; volume: "13(11):e1700662. doi"; pages: " 
            10.1002/biot.201700662. Epub 2018 Apr 30"
COMMENT     Sequence Label: pBsaGSCYP101A1
FEATURES             Location/Qualifiers
     source          1..3504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..24,25..31)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    39..55
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     44..66
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     63..79
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     63..79
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1425..1442)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1425..1441)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1434..1456)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1596..1613)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1767..2355)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1847..1866)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2498..2600
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2601..3260
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2667..2686)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    3469..3490
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
       61 cacaggaaac agctatgacc atgattacga attcgagctc gggtctcggg aggtagcatg
      121 actaccgaaa ccatccagtc taacgcgaac ctggctccgc tgccgccgca tgtgccggaa
      181 cacctggtgt tcgacttcga tatgtacaac ccgtctaacc tgtctgcggg cgttcaggaa
      241 gcgtgggcgg tgctgcagga atccaacgtt ccggatctgg tgtggacccg ctgcaacggc
      301 ggtcactgga tcgcgacccg tggccagctg attcgtgaag catacgaaga ttaccgtcat
      361 ttctcctctg aatgcccgtt catcccgcgt gaggctggtg aggcgtacga tttcattccg
      421 actagcatgg acccgccgga gcagcgccag tttcgtgcgc tggcgaacca ggttgttggt
      481 atgccggttg tggataaact ggagaaccgt atccaggagc tggcgtgctc tctgattgag
      541 tctctgcgtc cgcagggtca gtgcaacttc accgaagact acgcggaacc gtttccgatc
      601 cgtattttca tgctgctggc gggtctgccg gaggaagaca ttccgcacct gaagtatctg
      661 accgaccaga tgactcgccc ggatggctct atgaccttcg cggaggcgaa agaagcgctg
      721 tacgactacc tgatcccgat catcgaacag cgccgtcaga agccgggtac cgacgcgatc
      781 tctatcgttg cgaacggtca ggttaacggt cgtccgatta cctccgatga agcgaaacgt
      841 atgtgcggtc tgctgctggt gggtggtctg gacactgttg ttaacttcct gtctttctct
      901 atggaatttc tggctaaaag cccggaacat cgtcaggaac tgatcgagcg tccggagcgc
      961 attccggctg cgtgcgaaga actgctgcgt cgcttctctc tggttgcaga cggtcgcatc
     1021 ctgacctctg actacgagtt ccacggtgtg cagctgaaaa aaggtgatca gatcctgctg
     1081 ccgcagatgc tgagcggcct ggacgaacgt gaaaacgcgt gtccgatgca cgttgacttt
     1141 tctcgccaga aggtttctca cactactttc ggtcacggct ctcacctgtg cctgggccag
     1201 cacctggcgc gtcgcgagat cattgttacc ctgaaagaat ggctgactcg tatcccggat
     1261 ttcagcattg caccgggtgc gcagatccag cacaaatctg gtatcgttag cggcgtgcag
     1321 gctctgccgc tggtttggga cccggcaacc actaaagcgg tttgaggatc tgagaccgta
     1381 cccggggatc ctctagagtc gacctgcagg catgcaagct tggcactggc cgtcgtttta
     1441 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     1501 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     1561 cgcagcctga atggcgaatg agcttcttcc gcttcctcgc tcactgactc gctgcgctcg
     1621 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1681 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1741 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1801 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1861 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1921 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1981 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2041 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2101 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2161 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     2221 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2281 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2341 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2401 tccgtcgaac ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg
     2461 ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca
     2521 actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt
     2581 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
     2641 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
     2701 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
     2761 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaatttc
     2821 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
     2881 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
     2941 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
     3001 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
     3061 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
     3121 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
     3181 ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
     3241 agtggcaggg cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctggtt
     3301 gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcagct tggcccagtg
     3361 ccaagctcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     3421 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     3481 gctcactcat taggcacccc aggc
//
