LOCUS       sequence-208364-         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of E.coli DHFR Y100F mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Liu CT, Francis K, Layfield JP, Huang X, Hammes-Schiffer S, Kohen A,
            Benkovic SJ
  TITLE     Escherichia coli dihydrofolate reductase catalyzed proton and 
            hydride transfers: temporal order and the roles of Asp27 and Tyr100.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Dec 23;111(51):18231-6. doi: 
            10.1073/pnas.1415940111. Epub 2014 Dec 1.
  PUBMED    25453098
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1415940111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-12-23- 23"; volume: "111"; issue: "51"; pages: "18231-6"
COMMENT     Sequence Label: pET22b-ecDHFR-Y100F
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             562..579
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata tacatatgat cagtctgatt gcggcgctag
       61 cggtagatcg cgttatcggc atggaaaacg ccatgccatg gaacctgcct gccgatctcg
      121 cctggtttaa acgcaacacc ttaaataaac ccgtgattat ggggcgccat acctgggaat
      181 caatcggtag gcctttgccc ggccgcaaaa atattatcct cagcagtcaa cccgggaccg
      241 atgatcgggt tacctgggtt aaatcggtcg acgaagccat cgcggcgtgt ggtgacgtac
      301 cagaaatcat ggtgattggc ggcggacgcg tttttgaaca gttcttgcca aaagcgcaaa
      361 aactgtatct gacgcatatc gatgcagaag tggaaggcga cacccatttt ccggattacg
      421 agccggatga ctgggaatcg gtattcagcg aattccacga tgctgatgcg cagaactcgc
      481 atagctattg tttcgaaatc ctcgagcgtc gttaaggatc cgaattcgag ctccgtcgac
      541 aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa
      601 gcccgaaagg aagct
//
