LOCUS       sequence-208368-       11158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SCRIB - Proline to Lysine mutation at 305 a.a. in MSCV backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11158)
  AUTHORS   Zheng W, Umitsu M, Jagan I, Tran CW, Ishiyama N, BeGora M, Araki K, 
            Ohashi PS, Ikura M, Muthuswamy SK
  TITLE     An interaction between Scribble and the NADPH oxidase complex 
            controls M1 macrophage polarization and function.
  JOURNAL   Nat Cell Biol. 2016 Nov;18(11):1244-1252. doi: 10.1038/ncb3413. Epub
            2016 Oct 3.
  PUBMED    27694890
REFERENCE   2  (bases 1 to 11158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3413"; 
            journalName: "Nat Cell Biol"; date: "2016-11"; volume: "18"; issue: 
            "11"; pages: "1244-1252"
COMMENT     Sequence Label: MSCV Puro SCRIB P305L
FEATURES             Location/Qualifiers
     source          1..11158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     180..202
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     330..349
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             549..1065
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    1129..1470
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1537..1953
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1883..1905
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1921..1937
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             3434..3445
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6882..7381
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6917..6940)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7314..7333
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7402..8001
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7402..7421)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7898..7918
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8101..8615
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    8777..8793
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8801..8807,8808..8825,8826..8831))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8846..8867
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8984..9001)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9155..9743)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9235..9254)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9914..10705,10706..10774))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10537..10556
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10775..10879)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10947..10965
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11003..11025)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11125..11144
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
       61 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      121 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      181 ccagtcacga cgttgtaaaa cgacggcgca aggaatggtg catgcaagga gatggcgccc
      241 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
      301 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
      361 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggcg attagtccaa
      421 tttgttaaag acaggatatc agtggtccag gctctagttt tgactcaaca atatcaccag
      481 ctgaagccta tagagtacga gccatagata aaataaaaga ttttatttag tctccagaaa
      541 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt
      601 tgcaaggcat ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag
      661 agagacagca gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
      721 agggccaaga acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca
      781 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac
      841 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag
      901 cccacaaccc ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt
      961 attcccaata aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag
     1021 ggtctcctca gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga
     1081 gatttggaga cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc
     1141 ggtcgtttcg tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc
     1201 ctgcgtctgt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
     1261 gagttctgaa cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt
     1321 tgtggcccga cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt
     1381 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt
     1441 tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc
     1501 tgtctgactg tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta
     1561 agtttgacct taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat
     1621 gtcaagaaga gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga
     1681 tggccgcgag acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt
     1741 tcacctggcc cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg
     1801 gcttttgacc cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt
     1861 cctccatccg ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc
     1921 ctttatccag ccctcactcc ttctctaggc gccggaatta gatctcgcca ccatgctcaa
     1981 gtgcatcccg ctgtggcgct gcaaccggca cgtggagtcg gtggacaagc ggcactgttc
     2041 gctgcaggcc gtgccggagg agatctaccg ctacagccgc agcctggagg agctgctgct
     2101 cgacgccaac cagctgcgcg agctgcccaa gccttttttc cggctgctga acttgcgcaa
     2161 gctgggcctg agcgacaacg agatccagcg gttgcctccc gaggtggcca acttcatgca
     2221 gctggtggag ctggacgtgt cccggaacga tatccctgag atcccggaga gcatcaagtt
     2281 ctgcaaggct ctggagatcg cggacttcag tgggaacccc ctctccaggc tccctgatgg
     2341 cttcactcag ctgcgcagcc tggctcacct ggccctgaat gatgtgtctc tgcaggcact
     2401 gcccggggac gtgggcaacc tcgccaacct ggtgaccctg gagctccggg agaacctgct
     2461 caagtccctg ccagcgtccc tgtcatttct ggtcaagctg gaacagctgg atctgggagg
     2521 caacgatctg gaagtgctgc cagacactct gggggctctg cccaatcttc gggagctgtg
     2581 gcttgaccgg aaccagctgt cagcactgcc cccggagctc gggaacctgc ggcgcctggt
     2641 gtgcctggac gtgtcggaaa accggctgga ggagctgcct gctgagctcg gcgggctggt
     2701 gctgctcact gacctgctgc tgtcccagaa cctgctgcgg aggctgcccg acggcatcgg
     2761 tcagctgaag cagctatcca tcctaaaggt agaccagaat cggctgtgcg aggtgactga
     2821 ggccatcggg gactgtgaga acctctctga gctgatcctc acggagaacc tgctgatggc
     2881 cctgctccgc tccctgggaa agctgactaa gctgaccaac ctcaacgtgg accggaacca
     2941 cctcgaggcg ctgccgcccg agatcggggg ctgtgtggca ctcagcgtcc tctccttgag
     3001 ggacaaccgc ctggccgtcc tgccaccaga gctggcccac acgacagagc tgcacgtgct
     3061 ggacgtggcg gggaaccgcc tgcagagtct gccgttcgcg ctcacccacc tcaatctcaa
     3121 ggccctgtgg ctggcagaga accaggcgca gcccatgctc cggttccaga cggaggatga
     3181 tgcccggacc ggcgagaagg tgctcacctg ctacttgctg ccccagcagc ccccacccag
     3241 cctcgaggat gctgggcagc aggggagcct ctcggagacc tggagcgatg ccccgccgag
     3301 ccgcgtcagc gtcatccagt tcctggaggc ccccataggt gatgaggacg ctgaggaagc
     3361 tgcagctgag aagcggggcc tacagcgccg ggccacacct caccccagcg agctcaaggt
     3421 gatgaagagg agcatcgagg ggcggcggag cgaggcctgc ccttgccagc cagactctgg
     3481 gtcgcccttg cctgcagagg aggagaagcg gctgagtgcc gagtctggcc tgagtgaaga
     3541 ctctcgccca tctgccagca cagtctctga ggctgagccc gagggcccgt cggctgaggc
     3601 acagggtggg agccagcagg aagccacgac tgctggcggg gaggaagacg ccgaagagga
     3661 ctaccaggag cccacggtgc atttcgcaga ggacgcactg ctgcccgggg atgacaggga
     3721 gatcgaggag gggcagcctg aggccccctg gaccctgcca ggcgggaggc agcggctcat
     3781 ccgcaaggac acacctcact acaaaaagca cttcaagatc tccaagctgc cccagcccga
     3841 ggccgttgtg gctctgctgc agggcatgca gcctgatggg gagggccctg tggctcccgg
     3901 gggctggcac aatggccccc acgcaccctg ggctcctcgg gcccagaagg aggaggagga
     3961 ggaggaagag ggtagtcctc aggaggagga ggaagaggag gaggaggaaa acagggctga
     4021 agaggaagag gccagcactg aggaggagga caaggagggg gccgtggttt ctgcgccctc
     4081 tgtcaaggga gtgtcgtttg accaggccaa taacctgctg atagagcctg ctcgcattga
     4141 ggaggaagag ctgaccctca ccatcctgcg gcagactggg ggcctgggca tcagcattgc
     4201 gggcggcaag ggctccacac cctataaggg ggacgacgag ggcatattca tctctcgggt
     4261 gtccgaggaa ggccctgcgg cccgggctgg agtccgtgtg ggtgacaagc tcctggaggt
     4321 gaatggtgtg gctctgcagg gcgccgagca ccacgaggcc gtggaggcgc tccggggggc
     4381 cggcactgcc gtgcagatgc gagtgtggcg ggagcgcatg gtggagcctg agaacgcggt
     4441 caccatcacg ccgctgcggc ccgaggatga ttacagcccc cgagagcggc ggggaggggg
     4501 gctgcgcctg cccctgctcc cgcctgagag ccccgggccc ctccgtcagc gccacgtggc
     4561 ctgcctggca cgcagcgaga gggggctggg cttcagcatt gctggtggga aaggctccac
     4621 accctacagg gctggtgatg cgggcatctt cgtctcccgc attgccgagg gcggtgctgc
     4681 tcaccgcgcg ggcacactgc aggttggcga ccgcgtcctc tctattaatg gagtggacgt
     4741 gactgaggcc aggcatgacc acgccgtctc cctgctgacc gctgcctccc ccaccatcgc
     4801 cctgctgttg gagcgggagg ctgggggccc tcttcctccc agccctctgc cacattcctc
     4861 accccccacc gctgctgttg ccaccaccag cataaccact gccacccccg gggtgcctgg
     4921 gttgccgagc ctggccccca gcctgctggc tgccgcgttg gaagggccat acccagtgga
     4981 ggagatccgt ctgccaagag ctgggggccc tctggggctt agtattgtcg gaggctccga
     5041 ccattccagc cacccgtttg gtgtccagga gcctggtgtg ttcatctcca aggtgctccc
     5101 gcggggcctg gccgctcgca gcggcctgcg ggttggggac cgcatcctgg cagtgaacgg
     5161 gcaagacgtg cgggatgcca cgcaccaaga agcagtcagt gccctgctcc ggccctgcct
     5221 ggagctgtcg ctgctggtgc ggagggaccc ggcacccccg ggcctacggg aactgtgcat
     5281 ccagaaggca cctggggaga ggctgggcat cagcatccgc gggggtgcca ggggccacgc
     5341 tggcaacccc cgcgacccca cagacgaggg catcttcatc tccaaggtga gccccacggg
     5401 ggcagccggg cgcgacggtc ggctgcgtgt gggtttgcgg ctgttggagg tgaaccagca
     5461 gagcctgctg ggcctgacgc acggcgaggc ggtgcagctg ctccgcagtg tgggcgacac
     5521 cctcaccgtg ctggtctgtg acggcttcga ggccagcacc gacgcagccc tggaggtgtc
     5581 cccaggtgtc attgccaacc cctttgcggc aggcatcggc caccggaaca gcctggagag
     5641 catctcttcc atcgaccggg agctgagccc tgagggccca ggcaaggaga aggagctgcc
     5701 tggacagacc ctgcactggg ggcccgaggc cacagaagcc gcaggtcggg gtctgcagcc
     5761 cctgaagctg gactaccgcg ccctggccgc cgtgcccagc gctggcagcg tgcagagggt
     5821 accgtctgga gcagctggag ggaagatggc tgaatctccc tgctccccta gtggccagca
     5881 gccgccctcc ccgccttctc cggatgagct gcccgccaat gtgaagcagg cctacagggc
     5941 cttcgcggcc gtgcccactt ctcacccgcc tgaggatgcc cctgcccagc cccccacgcc
     6001 tgggcctgca gcctccccgg agcagctgtc cttccgggag cggcagaagt actttgagct
     6061 ggaggtgcgc gtgccccagg ccgagggccc ccctaagcgc gtgtccctgg tgggtgctga
     6121 cgacctgcgg aagatgcagg aggaggaagc cagaaaacta cagcagaaga gagcgcagat
     6181 gctgcgggag gcggcagagg ctggggccga agcgaggctc gccctggacg gggagacgct
     6241 gggcgaggag gaacaggagg atgagcagcc accctgggcc agcccgagcc ccacctcaag
     6301 gcagagcccg gcgtcccccc cgcccctggg aggtggcgcc ccggtgcgga cggccaaagc
     6361 tgaacggcgc caccaggagc ggctgcgcgt gcagagtccg gagccaccgg cacccgagcg
     6421 tgccctgtcc cctgccgagc tccgggccct ggaggccgag aagcgtgcgc tgtggagggc
     6481 agccaggatg aagtcattgg aacaggacgc tctccgagca cagatggtcc tcagcaggtc
     6541 ccaggaaggc cggggcacgc gggggcccct ggagcgactg gccgaggccc cttcccctgc
     6601 gcccaccccg tcgcccaccc ctgtggaaga ccttggcccc cagaccagca cctccccggg
     6661 acgcctgtca ccggactttg ctgaggagtt gaggtccctg gaaccatctc ccagccctgg
     6721 cccgcaggag gaggatggag aagtggctct ggtgcttctg ggcaggccct cacccggcgc
     6781 tgtgggccct gaagatgtgg cactgtgcag cagccgccgc cccgtaaggc ctgggcgccg
     6841 tggcctgggc cctgtgccct cctagggtta agaattctac cgggtagggg aggcgctttt
     6901 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
     6961 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
     7021 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
     7081 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
     7141 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
     7201 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
     7261 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
     7321 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc
     7381 gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     7441 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     7501 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     7561 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     7621 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     7681 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     7741 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     7801 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     7861 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     7921 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     7981 acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc
     8041 gcacgacccc atgcatcgat aaaataaaag attttattta gtctccagaa aaagggggga
     8101 atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca
     8161 tggaaaatac ataactgaga atagagaagt tcagatcaag gttaggaaca gagagacagc
     8221 agaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     8281 aacagatggt ccccagatgc ggtcccgccc tcagcagttt ctagagaacc atcagatgtt
     8341 tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
     8401 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc
     8461 cctcactcgg cgcgccagtc ctccgataga ctgcgtcgcc cgggtacccg tgtatccaat
     8521 aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct
     8581 gagtgattga ctacccgtca gcgggggtct ttcatgggta acagtttctt gaagttggag
     8641 aacaacattc tgagggtagg agtcgaatat taagtaatcc tgactcaatt agccactgtt
     8701 ttgaatccac atactccaat actcctgaaa tagttcatta tggacagcgc agaaagagct
     8761 ggggagaatt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     8821 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     8881 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8941 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     9001 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     9061 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     9121 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     9181 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     9241 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9301 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     9361 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     9421 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     9481 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     9541 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     9601 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9661 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9721 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9781 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     9841 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9901 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9961 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    10021 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    10081 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    10141 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    10201 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    10261 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    10321 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    10381 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    10441 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    10501 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    10561 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    10621 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    10681 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10741 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10801 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    10861 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
    10921 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
    10981 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
    11041 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
    11101 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcacca
//
