LOCUS       sequence-208387-        8583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV expression vector for BE3-2X construct (not codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8583)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 8583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194."
COMMENT     Sequence Label: pCMV-BE3-2X
FEATURES             Location/Qualifiers
     source          1..8583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             409..1095
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             1114..1134
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1144..1164
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1195..4593,4594..4596,4597..5193,5194,5195..5197,
                     5198..5199,5200..5202,5203..5295)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1195..1218,1219..1221,1222..5295)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5308..5556
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5569..5589
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5598..5615
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5638..5655)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5644..5868
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5939..5955)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5939..5955)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5952..5974)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5963..5979
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5987..5993,5994..6011,6012..6017))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6032..6053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6170..6187)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6341..6929)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6421..6440)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7100..7891,7892..7960))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7723..7742
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7961..8065)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8144..8163)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8335..8583,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gagctcagag
      421 actggcccag tggctgtgga ccccacattg agacggcgga tcgagcccca tgagtttgag
      481 gtattcttcg atccgagaga gctccgcaag gagacctgcc tgctttacga aattaattgg
      541 gggggccggc actccatttg gcgacataca tcacagaaca ctaacaagca cgtcgaagtc
      601 aacttcatcg agaagttcac gacagaaaga tatttctgtc cgaacacaag gtgcagcatt
      661 acctggtttc tcagctggag cccatgcggc gaatgtagta gggccatcac tgaattcctg
      721 tcaaggtatc cccacgtcac tctgtttatt tacatcgcaa ggctgtacca ccacgctgac
      781 ccccgcaatc gacaaggcct gcgggatttg atctcttcag gtgtgactat ccaaattatg
      841 actgagcagg agtcaggata ctgctggaga aactttgtga attatagccc gagtaatgaa
      901 gcccactggc ctaggtatcc ccatctgtgg gtacgactgt acgttcttga actgtactgc
      961 atcatactgg gcctgcctcc ttgtctcaac attctgagaa ggaagcagcc acagctgaca
     1021 ttctttacca tcgctcttca gtcttgtcat taccagcgac tgcccccaca cattctctgg
     1081 gccaccgggt tgaaaagcgg cagcgagact cccccaaaga agaaacggaa agtaggcggc
     1141 tcccccaaga agaagcggaa ggtagggacc tcagagtccg ccacacccga aagtgataaa
     1201 aagtattcta ttggtttagc catcggcact aattccgttg gatgggctgt cataaccgat
     1261 gaatacaaag taccttcaaa gaaatttaag gtgttgggga acacagaccg tcattcgatt
     1321 aaaaagaatc ttatcggtgc cctcctattc gatagtggcg aaacggcaga ggcgactcgc
     1381 ctgaaacgaa ccgctcggag aaggtataca cgtcgcaaga accgaatatg ttacttacaa
     1441 gaaattttta gcaatgagat ggccaaagtt gacgattctt tctttcaccg tttggaagag
     1501 tccttccttg tcgaagagga caagaaacat gaacggcacc ccatctttgg aaacatagta
     1561 gatgaggtgg catatcatga aaagtaccca acgatttatc acctcagaaa aaagctagtt
     1621 gactcaactg ataaagcgga cctgaggtta atctacttgg ctcttgccca tatgataaag
     1681 ttccgtgggc actttctcat tgagggtgat ctaaatccgg acaactcgga tgtcgacaaa
     1741 ctgttcatcc agttagtaca aacctataat cagttgtttg aagagaaccc tataaatgca
     1801 agtggcgtgg atgcgaaggc tattcttagc gcccgcctct ctaaatcccg acggctagaa
     1861 aacctgatcg cacaattacc cggagagaag aaaaatgggt tgttcggtaa ccttatagcg
     1921 ctctcactag gcctgacacc aaattttaag tcgaacttcg acttagctga agatgccaaa
     1981 ttgcagctta gtaaggacac gtacgatgac gatctcgaca atctactggc acaaattgga
     2041 gatcagtatg cggacttatt tttggctgcc aaaaacctta gcgatgcaat cctcctatct
     2101 gacatactga gagttaatac tgagattacc aaggcgccgt tatccgcttc aatgatcaaa
     2161 aggtacgatg aacatcacca agacttgaca cttctcaagg ccctagtccg tcagcaactg
     2221 cctgagaaat ataaggaaat attctttgat cagtcgaaaa acgggtacgc aggttatatt
     2281 gacggcggag cgagtcaaga ggaattctac aagtttatca aacccatatt agagaagatg
     2341 gatgggacgg aagagttgct tgtaaaactc aatcgcgaag atctactgcg aaagcagcgg
     2401 actttcgaca acggtagcat tccacatcaa atccacttag gcgaattgca tgctatactt
     2461 agaaggcagg aggattttta tccgttcctc aaagacaatc gtgaaaagat tgagaaaatc
     2521 ctaacctttc gcatacctta ctatgtggga cccctggccc gagggaactc tcggttcgca
     2581 tggatgacaa gaaagtccga agaaacgatt actccatgga attttgagga agttgtcgat
     2641 aaaggtgcgt cagctcaatc gttcatcgag aggatgacca actttgacaa gaatttaccg
     2701 aacgaaaaag tattgcctaa gcacagttta ctttacgagt atttcacagt gtacaatgaa
     2761 ctcacgaaag ttaagtatgt cactgagggc atgcgtaaac ccgcctttct aagcggagaa
     2821 cagaagaaag caatagtaga tctgttattc aagaccaacc gcaaagtgac agttaagcaa
     2881 ttgaaagagg actactttaa gaaaattgaa tgcttcgatt ctgtcgagat ctccggggta
     2941 gaagatcgat ttaatgcgtc acttggtacg tatcatgacc tcctaaagat aattaaagat
     3001 aaggacttcc tggataacga agagaatgaa gatatcttag aagatatagt gttgactctt
     3061 accctctttg aagatcggga aatgattgag gaaagactaa aaacatacgc tcacctgttc
     3121 gacgataagg ttatgaaaca gttaaagagg cgtcgctata cgggctgggg acgattgtcg
     3181 cggaaactta tcaacgggat aagagacaag caaagtggta aaactattct cgattttcta
     3241 aagagcgacg gcttcgccaa taggaacttt atgcagctga tccatgatga ctctttaacc
     3301 ttcaaagagg atatacaaaa ggcacaggtt tccggacaag gggactcatt gcacgaacat
     3361 attgcgaatc ttgctggttc gccagccatc aaaaagggca tactccagac agtcaaagta
     3421 gtggatgagc tagttaaggt catgggacgt cacaaaccgg aaaacattgt aatcgagatg
     3481 gcacgcgaaa atcaaacgac tcagaagggg caaaaaaaca gtcgagagcg gatgaagaga
     3541 atagaagagg gtattaaaga actgggcagc cagatcttaa aggagcatcc tgtggaaaat
     3601 acccaattgc agaacgagaa actttacctc tattacctac aaaatggaag ggacatgtat
     3661 gttgatcagg aactggacat aaaccgttta tctgattacg acgtcgatca cattgtaccc
     3721 caatcctttt tgaaggacga ttcaatcgac aataaagtgc ttacacgctc ggataagaac
     3781 cgagggaaaa gtgacaatgt tccaagcgag gaagtcgtaa agaaaatgaa gaactattgg
     3841 cggcagctcc taaatgcgaa actgataacg caaagaaagt tcgataactt aactaaagct
     3901 gagaggggtg gcttgtctga acttgacaag gccggattta ttaaacgtca gctcgtggaa
     3961 acccgccaaa tcacaaagca tgttgcacag atactagatt cccgaatgaa tacgaaatac
     4021 gacgagaacg ataagctgat tcgggaagtc aaagtaatca ctttaaagtc aaaattggtg
     4081 tcggacttca gaaaggattt tcaattctat aaagttaggg agataaataa ctaccaccat
     4141 gcgcacgacg cttatcttaa tgccgtcgta gggaccgcac tcattaagaa atacccgaag
     4201 ctagaaagtg agtttgtgta tggtgattac aaagtttatg acgtccgtaa gatgatcgcg
     4261 aaaagcgaac aggagatagg caaggctaca gccaaatact tcttttattc taacattatg
     4321 aatttcttta agacggaaat cactctggca aacggagaga tacgcaaacg acctttaatt
     4381 gaaaccaatg gggagacagg tgaaatcgta tgggataagg gccgggactt cgcgacggtg
     4441 agaaaagttt tgtccatgcc ccaagtcaac atagtaaaga aaactgaggt gcagaccgga
     4501 gggttttcaa aggaatcgat tcttccaaaa aggaatagtg ataagctcat cgctcgtaaa
     4561 aaggactggg acccgaaaaa gtacggtggc ttcgatagcc ctacagttgc ctattctgtc
     4621 ctagtagtgg caaaagttga gaagggaaaa tccaagaaac tgaagtcagt caaagaatta
     4681 ttggggataa cgattatgga gcgctcgtct tttgaaaaga accccatcga cttccttgag
     4741 gcgaaaggtt acaaggaagt aaaaaaggat ctcataatta aactaccaaa gtatagtctg
     4801 tttgagttag aaaatggccg aaaacggatg ttggctagcg ccggagagct tcaaaagggg
     4861 aacgaactcg cactaccgtc taaatacgtg aatttcctgt atttagcgtc ccattacgag
     4921 aagttgaaag gttcacctga agataacgaa cagaagcaac tttttgttga gcagcacaaa
     4981 cattatctcg acgaaatcat agagcaaatt tcggaattca gtaagagagt catcctagct
     5041 gatgccaatc tggacaaagt attaagcgca tacaacaagc acagggataa acccatacgt
     5101 gagcaggcgg aaaatattat ccatttgttt actcttacca acctcggcgc tccagccgca
     5161 ttcaagtatt ttgacacaac gatagatcgc aaacgataca cttctaccaa ggaggtgcta
     5221 gacgcgacac tgattcacca atccatcacg ggattatatg aaactcggat agatttgtca
     5281 cagcttgggg gtgactctgg tggttctact aatctgtcag atattattga aaaggagacc
     5341 ggtaagcaac tggttatcca ggaatccatc ctcatgctcc cagaggaggt ggaagaagtc
     5401 attgggaaca agccggaaag cgatatactc gtgcacaccg cctacgacga gagcaccgac
     5461 gagaatgtca tgcttctgac tagcgacgcc cctgaataca agccttgggc tctggtcata
     5521 caggatagca acggtgagaa caagattaag atgctctctg gtggttctcc caagaagaag
     5581 aggaaagtct aaccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct
     5641 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     5701 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     5761 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     5821 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     5881 aaagaaccag ctggggctcg ataccgtcga cctctagcta gagcttggcg taatcatggt
     5941 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6001 gaagcataaa gtgtaaagcc tagggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6061 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6121 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6181 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6241 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6301 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6361 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6421 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6481 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6541 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6601 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6661 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6721 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6781 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6841 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6901 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6961 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7021 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7081 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7141 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7201 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7261 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7321 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7381 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7441 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7501 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7561 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7621 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7681 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7741 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7801 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7861 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7921 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7981 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8041 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     8101 gatcgggaga tcgatctccc gatcccctag ggtcgactct cagtacaatc tgctctgatg
     8161 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     8221 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
     8281 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
     8341 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     8401 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     8461 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     8521 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     8581 atc
//
