LOCUS       sequence-208388-        8644 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV expression vector for BE3-FNLS construct (not codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8644)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 8644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194."
COMMENT     Sequence Label: pCMV-BE3-FNLS
FEATURES             Location/Qualifiers
     source          1..8644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     369..388
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        369..387
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      395..404
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             404..469
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             476..496
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             521..1207
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(1256..4654,4655..4657,4658..5254,5255,5256..5258,
                     5259..5260,5261..5263,5264..5356)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1256..1279,1280..1282,1283..5356)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5369..5617
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5630..5650
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5659..5676
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5699..5716)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5705..5929
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6000..6016)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6000..6016)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6013..6035)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6024..6040
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6048..6054,6055..6072,6073..6078))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6093..6114
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6231..6248)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6402..6990)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6482..6501)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7161..7952,7953..8021))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7784..7803
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8022..8126)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8205..8224)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8396..8644,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccta atacgactca ctatagggag agccgccacc atggactata aggaccacga
      421 cggagactac aaggatcatg atattgatta caaagacgat gacgataaga tggccccaaa
      481 gaagaagcgg aaggtcggta tccacggagt cccagcagcc atgagctcag agactggccc
      541 agtggctgtg gaccccacat tgagacggcg gatcgagccc catgagtttg aggtattctt
      601 cgatccgaga gagctccgca aggagacctg cctgctttac gaaattaatt gggggggccg
      661 gcactccatt tggcgacata catcacagaa cactaacaag cacgtcgaag tcaacttcat
      721 cgagaagttc acgacagaaa gatatttctg tccgaacaca aggtgcagca ttacctggtt
      781 tctcagctgg agcccatgcg gcgaatgtag tagggccatc actgaattcc tgtcaaggta
      841 tccccacgtc actctgttta tttacatcgc aaggctgtac caccacgctg acccccgcaa
      901 tcgacaaggc ctgcgggatt tgatctcttc aggtgtgact atccaaatta tgactgagca
      961 ggagtcagga tactgctgga gaaactttgt gaattatagc ccgagtaatg aagcccactg
     1021 gcctaggtat ccccatctgt gggtacgact gtacgttctt gaactgtact gcatcatact
     1081 gggcctgcct ccttgtctca acattctgag aaggaagcag ccacagctga cattctttac
     1141 catcgctctt cagtcttgtc attaccagcg actgccccca cacattctct gggccaccgg
     1201 gttgaaaagc ggcagcgaga ctcccgggac ctcagagtcc gccacacccg aaagtgataa
     1261 aaagtattct attggtttag ccatcggcac taattccgtt ggatgggctg tcataaccga
     1321 tgaatacaaa gtaccttcaa agaaatttaa ggtgttgggg aacacagacc gtcattcgat
     1381 taaaaagaat cttatcggtg ccctcctatt cgatagtggc gaaacggcag aggcgactcg
     1441 cctgaaacga accgctcgga gaaggtatac acgtcgcaag aaccgaatat gttacttaca
     1501 agaaattttt agcaatgaga tggccaaagt tgacgattct ttctttcacc gtttggaaga
     1561 gtccttcctt gtcgaagagg acaagaaaca tgaacggcac cccatctttg gaaacatagt
     1621 agatgaggtg gcatatcatg aaaagtaccc aacgatttat cacctcagaa aaaagctagt
     1681 tgactcaact gataaagcgg acctgaggtt aatctacttg gctcttgccc atatgataaa
     1741 gttccgtggg cactttctca ttgagggtga tctaaatccg gacaactcgg atgtcgacaa
     1801 actgttcatc cagttagtac aaacctataa tcagttgttt gaagagaacc ctataaatgc
     1861 aagtggcgtg gatgcgaagg ctattcttag cgcccgcctc tctaaatccc gacggctaga
     1921 aaacctgatc gcacaattac ccggagagaa gaaaaatggg ttgttcggta accttatagc
     1981 gctctcacta ggcctgacac caaattttaa gtcgaacttc gacttagctg aagatgccaa
     2041 attgcagctt agtaaggaca cgtacgatga cgatctcgac aatctactgg cacaaattgg
     2101 agatcagtat gcggacttat ttttggctgc caaaaacctt agcgatgcaa tcctcctatc
     2161 tgacatactg agagttaata ctgagattac caaggcgccg ttatccgctt caatgatcaa
     2221 aaggtacgat gaacatcacc aagacttgac acttctcaag gccctagtcc gtcagcaact
     2281 gcctgagaaa tataaggaaa tattctttga tcagtcgaaa aacgggtacg caggttatat
     2341 tgacggcgga gcgagtcaag aggaattcta caagtttatc aaacccatat tagagaagat
     2401 ggatgggacg gaagagttgc ttgtaaaact caatcgcgaa gatctactgc gaaagcagcg
     2461 gactttcgac aacggtagca ttccacatca aatccactta ggcgaattgc atgctatact
     2521 tagaaggcag gaggattttt atccgttcct caaagacaat cgtgaaaaga ttgagaaaat
     2581 cctaaccttt cgcatacctt actatgtggg acccctggcc cgagggaact ctcggttcgc
     2641 atggatgaca agaaagtccg aagaaacgat tactccatgg aattttgagg aagttgtcga
     2701 taaaggtgcg tcagctcaat cgttcatcga gaggatgacc aactttgaca agaatttacc
     2761 gaacgaaaaa gtattgccta agcacagttt actttacgag tatttcacag tgtacaatga
     2821 actcacgaaa gttaagtatg tcactgaggg catgcgtaaa cccgcctttc taagcggaga
     2881 acagaagaaa gcaatagtag atctgttatt caagaccaac cgcaaagtga cagttaagca
     2941 attgaaagag gactacttta agaaaattga atgcttcgat tctgtcgaga tctccggggt
     3001 agaagatcga tttaatgcgt cacttggtac gtatcatgac ctcctaaaga taattaaaga
     3061 taaggacttc ctggataacg aagagaatga agatatctta gaagatatag tgttgactct
     3121 taccctcttt gaagatcggg aaatgattga ggaaagacta aaaacatacg ctcacctgtt
     3181 cgacgataag gttatgaaac agttaaagag gcgtcgctat acgggctggg gacgattgtc
     3241 gcggaaactt atcaacggga taagagacaa gcaaagtggt aaaactattc tcgattttct
     3301 aaagagcgac ggcttcgcca ataggaactt tatgcagctg atccatgatg actctttaac
     3361 cttcaaagag gatatacaaa aggcacaggt ttccggacaa ggggactcat tgcacgaaca
     3421 tattgcgaat cttgctggtt cgccagccat caaaaagggc atactccaga cagtcaaagt
     3481 agtggatgag ctagttaagg tcatgggacg tcacaaaccg gaaaacattg taatcgagat
     3541 ggcacgcgaa aatcaaacga ctcagaaggg gcaaaaaaac agtcgagagc ggatgaagag
     3601 aatagaagag ggtattaaag aactgggcag ccagatctta aaggagcatc ctgtggaaaa
     3661 tacccaattg cagaacgaga aactttacct ctattaccta caaaatggaa gggacatgta
     3721 tgttgatcag gaactggaca taaaccgttt atctgattac gacgtcgatc acattgtacc
     3781 ccaatccttt ttgaaggacg attcaatcga caataaagtg cttacacgct cggataagaa
     3841 ccgagggaaa agtgacaatg ttccaagcga ggaagtcgta aagaaaatga agaactattg
     3901 gcggcagctc ctaaatgcga aactgataac gcaaagaaag ttcgataact taactaaagc
     3961 tgagaggggt ggcttgtctg aacttgacaa ggccggattt attaaacgtc agctcgtgga
     4021 aacccgccaa atcacaaagc atgttgcaca gatactagat tcccgaatga atacgaaata
     4081 cgacgagaac gataagctga ttcgggaagt caaagtaatc actttaaagt caaaattggt
     4141 gtcggacttc agaaaggatt ttcaattcta taaagttagg gagataaata actaccacca
     4201 tgcgcacgac gcttatctta atgccgtcgt agggaccgca ctcattaaga aatacccgaa
     4261 gctagaaagt gagtttgtgt atggtgatta caaagtttat gacgtccgta agatgatcgc
     4321 gaaaagcgaa caggagatag gcaaggctac agccaaatac ttcttttatt ctaacattat
     4381 gaatttcttt aagacggaaa tcactctggc aaacggagag atacgcaaac gacctttaat
     4441 tgaaaccaat ggggagacag gtgaaatcgt atgggataag ggccgggact tcgcgacggt
     4501 gagaaaagtt ttgtccatgc cccaagtcaa catagtaaag aaaactgagg tgcagaccgg
     4561 agggttttca aaggaatcga ttcttccaaa aaggaatagt gataagctca tcgctcgtaa
     4621 aaaggactgg gacccgaaaa agtacggtgg cttcgatagc cctacagttg cctattctgt
     4681 cctagtagtg gcaaaagttg agaagggaaa atccaagaaa ctgaagtcag tcaaagaatt
     4741 attggggata acgattatgg agcgctcgtc ttttgaaaag aaccccatcg acttccttga
     4801 ggcgaaaggt tacaaggaag taaaaaagga tctcataatt aaactaccaa agtatagtct
     4861 gtttgagtta gaaaatggcc gaaaacggat gttggctagc gccggagagc ttcaaaaggg
     4921 gaacgaactc gcactaccgt ctaaatacgt gaatttcctg tatttagcgt cccattacga
     4981 gaagttgaaa ggttcacctg aagataacga acagaagcaa ctttttgttg agcagcacaa
     5041 acattatctc gacgaaatca tagagcaaat ttcggaattc agtaagagag tcatcctagc
     5101 tgatgccaat ctggacaaag tattaagcgc atacaacaag cacagggata aacccatacg
     5161 tgagcaggcg gaaaatatta tccatttgtt tactcttacc aacctcggcg ctccagccgc
     5221 attcaagtat tttgacacaa cgatagatcg caaacgatac acttctacca aggaggtgct
     5281 agacgcgaca ctgattcacc aatccatcac gggattatat gaaactcgga tagatttgtc
     5341 acagcttggg ggtgactctg gtggttctac taatctgtca gatattattg aaaaggagac
     5401 cggtaagcaa ctggttatcc aggaatccat cctcatgctc ccagaggagg tggaagaagt
     5461 cattgggaac aagccggaaa gcgatatact cgtgcacacc gcctacgacg agagcaccga
     5521 cgagaatgtc atgcttctga ctagcgacgc ccctgaatac aagccttggg ctctggtcat
     5581 acaggatagc aacggtgaga acaagattaa gatgctctct ggtggttctc ccaagaagaa
     5641 gaggaaagtc taaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc
     5701 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     5761 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     5821 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     5881 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     5941 gaaagaacca gctggggctc gataccgtcg acctctagct agagcttggc gtaatcatgg
     6001 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     6061 ggaagcataa agtgtaaagc ctagggtgcc taatgagtga gctaactcac attaattgcg
     6121 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     6181 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     6241 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6301 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6361 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6421 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6481 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6541 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6601 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6661 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6721 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6781 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6841 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6901 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6961 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7021 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7081 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7141 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7201 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7261 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7321 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7381 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7441 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7501 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7561 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7621 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7681 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7741 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7801 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7861 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7921 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7981 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8041 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8101 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     8161 ggatcgggag atcgatctcc cgatccccta gggtcgactc tcagtacaat ctgctctgat
     8221 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     8281 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
     8341 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
     8401 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     8461 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     8521 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     8581 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     8641 tatc
//
