LOCUS       sequence-208394-       12419 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of BE3 (not codon 
            optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12419)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 12419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194."
COMMENT     Sequence Label: pLenti-BE3-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..12419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1685..1918
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2103..2147
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2445..2562
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2680..2891
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2942..3628
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(3677..7075,7076..7078,7079..7675,7676,7677..7679,
                     7680..7681,7682..7684,7685..7777)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(3677..3700,3701..3703,3704..7777)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7790..8038
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8051..8071
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8080..8097
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        8136..8635
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8171..8194)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8568..8587
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8656..9255
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8656..8675)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9152..9172
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9308..9896
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9361..9381)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9779..9790)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9899..9915)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9900..9916)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10425..10605
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(10667..11255)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10747..10766)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11426..12217,12218..12286))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12049..12068
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12287..12391)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct ggaggaggag
     1561 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     1621 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     1681 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     1741 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     1801 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1861 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1921 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1981 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     2041 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     2101 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     2161 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     2221 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     2281 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     2341 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
     2401 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     2461 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2521 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     2581 agcagagatc cagtttggtt agtaccgggc ccgggtcgac tagtgatcag tgtgagggag
     2641 tgtaaagctg gttttcgagt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
     2701 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
     2761 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2821 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2881 ccagaacaca ggtgtcgtga cgcggctaac tcgaacgcta gctgtgcgat cgtttgccac
     2941 catgagctca gagactggcc cagtggctgt ggaccccaca ttgagacggc ggatcgagcc
     3001 ccatgagttt gaggtattct tcgatccgag agagctccgc aaggagacct gcctgcttta
     3061 cgaaattaat tgggggggcc ggcactccat ttggcgacat acatcacaga acactaacaa
     3121 gcacgtcgaa gtcaacttca tcgagaagtt cacgacagaa agatatttct gtccgaacac
     3181 aaggtgcagc attacctggt ttctcagctg gagcccatgc ggcgaatgta gtagggccat
     3241 cactgaattc ctgtcaaggt atccccacgt cactctgttt atttacatcg caaggctgta
     3301 ccaccacgct gacccccgca atcgacaagg cctgcgggat ttgatctctt caggtgtgac
     3361 tatccaaatt atgactgagc aggagtcagg atactgctgg agaaactttg tgaattatag
     3421 cccgagtaat gaagcccact ggcctaggta tccccatctg tgggtacgac tgtacgttct
     3481 tgaactgtac tgcatcatac tgggcctgcc tccttgtctc aacattctga gaaggaagca
     3541 gccacagctg acattcttta ccatcgctct tcagtcttgt cattaccagc gactgccccc
     3601 acacattctc tgggccaccg ggttgaaaag cggcagcgag actcccggga cctcagagtc
     3661 cgccacaccc gaaagtgata aaaagtattc tattggttta gccatcggca ctaattccgt
     3721 tggatgggct gtcataaccg atgaatacaa agtaccttca aagaaattta aggtgttggg
     3781 gaacacagac cgtcattcga ttaaaaagaa tcttatcggt gccctcctat tcgatagtgg
     3841 cgaaacggca gaggcgactc gcctgaaacg aaccgctcgg agaaggtata cacgtcgcaa
     3901 gaaccgaata tgttacttac aagaaatttt tagcaatgag atggccaaag ttgacgattc
     3961 tttctttcac cgtttggaag agtccttcct tgtcgaagag gacaagaaac atgaacggca
     4021 ccccatcttt ggaaacatag tagatgaggt ggcatatcat gaaaagtacc caacgattta
     4081 tcacctcaga aaaaagctag ttgactcaac tgataaagcg gacctgaggt taatctactt
     4141 ggctcttgcc catatgataa agttccgtgg gcactttctc attgagggtg atctaaatcc
     4201 ggacaactcg gatgtcgaca aactgttcat ccagttagta caaacctata atcagttgtt
     4261 tgaagagaac cctataaatg caagtggcgt ggatgcgaag gctattctta gcgcccgcct
     4321 ctctaaatcc cgacggctag aaaacctgat cgcacaatta cccggagaga agaaaaatgg
     4381 gttgttcggt aaccttatag cgctctcact aggcctgaca ccaaatttta agtcgaactt
     4441 cgacttagct gaagatgcca aattgcagct tagtaaggac acgtacgatg acgatctcga
     4501 caatctactg gcacaaattg gagatcagta tgcggactta tttttggctg ccaaaaacct
     4561 tagcgatgca atcctcctat ctgacatact gagagttaat actgagatta ccaaggcgcc
     4621 gttatccgct tcaatgatca aaaggtacga tgaacatcac caagacttga cacttctcaa
     4681 ggccctagtc cgtcagcaac tgcctgagaa atataaggaa atattctttg atcagtcgaa
     4741 aaacgggtac gcaggttata ttgacggcgg agcgagtcaa gaggaattct acaagtttat
     4801 caaacccata ttagagaaga tggatgggac ggaagagttg cttgtaaaac tcaatcgcga
     4861 agatctactg cgaaagcagc ggactttcga caacggtagc attccacatc aaatccactt
     4921 aggcgaattg catgctatac ttagaaggca ggaggatttt tatccgttcc tcaaagacaa
     4981 tcgtgaaaag attgagaaaa tcctaacctt tcgcatacct tactatgtgg gacccctggc
     5041 ccgagggaac tctcggttcg catggatgac aagaaagtcc gaagaaacga ttactccatg
     5101 gaattttgag gaagttgtcg ataaaggtgc gtcagctcaa tcgttcatcg agaggatgac
     5161 caactttgac aagaatttac cgaacgaaaa agtattgcct aagcacagtt tactttacga
     5221 gtatttcaca gtgtacaatg aactcacgaa agttaagtat gtcactgagg gcatgcgtaa
     5281 acccgccttt ctaagcggag aacagaagaa agcaatagta gatctgttat tcaagaccaa
     5341 ccgcaaagtg acagttaagc aattgaaaga ggactacttt aagaaaattg aatgcttcga
     5401 ttctgtcgag atctccgggg tagaagatcg atttaatgcg tcacttggta cgtatcatga
     5461 cctcctaaag ataattaaag ataaggactt cctggataac gaagagaatg aagatatctt
     5521 agaagatata gtgttgactc ttaccctctt tgaagatcgg gaaatgattg aggaaagact
     5581 aaaaacatac gctcacctgt tcgacgataa ggttatgaaa cagttaaaga ggcgtcgcta
     5641 tacgggctgg ggacgattgt cgcggaaact tatcaacggg ataagagaca agcaaagtgg
     5701 taaaactatt ctcgattttc taaagagcga cggcttcgcc aataggaact ttatgcagct
     5761 gatccatgat gactctttaa ccttcaaaga ggatatacaa aaggcacagg tttccggaca
     5821 aggggactca ttgcacgaac atattgcgaa tcttgctggt tcgccagcca tcaaaaaggg
     5881 catactccag acagtcaaag tagtggatga gctagttaag gtcatgggac gtcacaaacc
     5941 ggaaaacatt gtaatcgaga tggcacgcga aaatcaaacg actcagaagg ggcaaaaaaa
     6001 cagtcgagag cggatgaaga gaatagaaga gggtattaaa gaactgggca gccagatctt
     6061 aaaggagcat cctgtggaaa atacccaatt gcagaacgag aaactttacc tctattacct
     6121 acaaaatgga agggacatgt atgttgatca ggaactggac ataaaccgtt tatctgatta
     6181 cgacgtcgat cacattgtac cccaatcctt tttgaaggac gattcaatcg acaataaagt
     6241 gcttacacgc tcggataaga accgagggaa aagtgacaat gttccaagcg aggaagtcgt
     6301 aaagaaaatg aagaactatt ggcggcagct cctaaatgcg aaactgataa cgcaaagaaa
     6361 gttcgataac ttaactaaag ctgagagggg tggcttgtct gaacttgaca aggccggatt
     6421 tattaaacgt cagctcgtgg aaacccgcca aatcacaaag catgttgcac agatactaga
     6481 ttcccgaatg aatacgaaat acgacgagaa cgataagctg attcgggaag tcaaagtaat
     6541 cactttaaag tcaaaattgg tgtcggactt cagaaaggat tttcaattct ataaagttag
     6601 ggagataaat aactaccacc atgcgcacga cgcttatctt aatgccgtcg tagggaccgc
     6661 actcattaag aaatacccga agctagaaag tgagtttgtg tatggtgatt acaaagttta
     6721 tgacgtccgt aagatgatcg cgaaaagcga acaggagata ggcaaggcta cagccaaata
     6781 cttcttttat tctaacatta tgaatttctt taagacggaa atcactctgg caaacggaga
     6841 gatacgcaaa cgacctttaa ttgaaaccaa tggggagaca ggtgaaatcg tatgggataa
     6901 gggccgggac ttcgcgacgg tgagaaaagt tttgtccatg ccccaagtca acatagtaaa
     6961 gaaaactgag gtgcagaccg gagggttttc aaaggaatcg attcttccaa aaaggaatag
     7021 tgataagctc atcgctcgta aaaaggactg ggacccgaaa aagtacggtg gcttcgatag
     7081 ccctacagtt gcctattctg tcctagtagt ggcaaaagtt gagaagggaa aatccaagaa
     7141 actgaagtca gtcaaagaat tattggggat aacgattatg gagcgctcgt cttttgaaaa
     7201 gaaccccatc gacttccttg aggcgaaagg ttacaaggaa gtaaaaaagg atctcataat
     7261 taaactacca aagtatagtc tgtttgagtt agaaaatggc cgaaaacgga tgttggctag
     7321 cgccggagag cttcaaaagg ggaacgaact cgcactaccg tctaaatacg tgaatttcct
     7381 gtatttagcg tcccattacg agaagttgaa aggttcacct gaagataacg aacagaagca
     7441 actttttgtt gagcagcaca aacattatct cgacgaaatc atagagcaaa tttcggaatt
     7501 cagtaagaga gtcatcctag ctgatgccaa tctggacaaa gtattaagcg catacaacaa
     7561 gcacagggat aaacccatac gtgagcaggc ggaaaatatt atccatttgt ttactcttac
     7621 caacctcggc gctccagccg cattcaagta ttttgacaca acgatagatc gcaaacgata
     7681 cacttctacc aaggaggtgc tagacgcgac actgattcac caatccatca cgggattata
     7741 tgaaactcgg atagatttgt cacagcttgg gggtgactct ggtggttcta ctaatctgtc
     7801 agatattatt gaaaaggaga ccggtaagca actggttatc caggaatcca tcctcatgct
     7861 cccagaggag gtggaagaag tcattgggaa caagccggaa agcgatatac tcgtgcacac
     7921 cgcctacgac gagagcaccg acgagaatgt catgcttctg actagcgacg cccctgaata
     7981 caagccttgg gctctggtca tacaggatag caacggtgag aacaagatta agatgctctc
     8041 tggtggttct cccaagaaga agaggaaagt ctaaccggtc atcatcacca tcaccattga
     8101 aaaccgctgt tcctaggaat cccgaggcct ctaccgggta ggggaggcgc ttttcccaag
     8161 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
     8221 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
     8281 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
     8341 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
     8401 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
     8461 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
     8521 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
     8581 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
     8641 cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     8701 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     8761 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     8821 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     8881 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     8941 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     9001 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     9061 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     9121 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     9181 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     9241 aagcccggtg cctgaaaacg ttgttgtttg gggttgaatt actcttctag accgcggcgc
     9301 gcccgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     9361 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     9421 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     9481 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     9541 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     9601 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     9661 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     9721 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     9781 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     9841 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     9901 ataccgtcga cctcgatcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     9961 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
    10021 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
    10081 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
    10141 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
    10201 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
    10261 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga
    10321 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc
    10381 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag
    10441 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
    10501 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
    10561 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatg tgagcaaaag
    10621 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    10681 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    10741 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    10801 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    10861 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    10921 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    10981 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    11041 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    11101 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    11161 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    11221 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    11281 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    11341 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    11401 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    11461 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    11521 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    11581 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    11641 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    11701 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    11761 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    11821 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    11881 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    11941 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    12001 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    12061 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    12121 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    12181 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    12241 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    12301 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    12361 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
