LOCUS       sequence-208401-       11188 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of Cas9-VRER (not 
            codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11188)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 11188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194."
COMMENT     Sequence Label: pLenti-VRER-P2A-Puro
FEATURES             Location/Qualifiers
     source          1..11188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2140..2149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2146..5547,5548..5550,5551..5796,5797..5799,5800..6147,
                     6148..6150,6151..6153,6154..6156,6157..6249)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/G1218R/R1335E/T1337R mutations"
                     /label=Cas9 VRER
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKEYRSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6256..6276
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6283..6348
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6373..6429
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6430..7029
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6430..6449)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6926..6946
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7045..7633
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7098..7118)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7516..7527)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7705..7938
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8016..8137
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8053..8072)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8107..8126
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8177..8312
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8332..8351)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8333..8351)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8361..8378)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8361..8377)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8370..8392)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8519..8974
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8606..8625)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8816..8837
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9000..9104
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9105..9173,9174..9965)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9323..9342)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10136..10724
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10625..10644
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10878..10895
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11012..11033
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11048..11053,11054..11071,11072..11078)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11086..11102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11091..11113
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11110..11126
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11110..11126
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11145..11165
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11147..11165
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgga taaaaagtat
     2161 tctattggtt tagacatcgg cactaattcc gttggatggg ctgtcataac cgatgaatac
     2221 aaagtacctt caaagaaatt taaggtgttg gggaacacag accgtcattc gattaaaaag
     2281 aatcttatcg gtgccctcct attcgatagt ggcgaaacgg cagaggcgac tcgcctgaaa
     2341 cgaaccgctc ggagaaggta tacacgtcgc aagaaccgaa tatgttactt acaagaaatt
     2401 tttagcaatg agatggccaa agttgacgat tctttctttc accgtttgga agagtccttc
     2461 cttgtcgaag aggacaagaa acatgaacgg caccccatct ttggaaacat agtagatgag
     2521 gtggcatatc atgaaaagta cccaacgatt tatcacctca gaaaaaagct agttgactca
     2581 actgataaag cggacctgag gttaatctac ttggctcttg cccatatgat aaagttccgt
     2641 gggcactttc tcattgaggg tgatctaaat ccggacaact cggatgtcga caaactgttc
     2701 atccagttag tacaaaccta taatcagttg tttgaagaga accctataaa tgcaagtggc
     2761 gtggatgcga aggctattct tagcgcccgc ctctctaaat cccgacggct agaaaacctg
     2821 atcgcacaat tacccggaga gaagaaaaat gggttgttcg gtaaccttat agcgctctca
     2881 ctaggcctga caccaaattt taagtcgaac ttcgacttag ctgaagatgc caaattgcag
     2941 cttagtaagg acacgtacga tgacgatctc gacaatctac tggcacaaat tggagatcag
     3001 tatgcggact tatttttggc tgccaaaaac cttagcgatg caatcctcct atctgacata
     3061 ctgagagtta atactgagat taccaaggcg ccgttatccg cttcaatgat caaaaggtac
     3121 gatgaacatc accaagactt gacacttctc aaggccctag tccgtcagca actgcctgag
     3181 aaatataagg aaatattctt tgatcagtcg aaaaacgggt acgcaggtta tattgacggc
     3241 ggagcgagtc aagaggaatt ctacaagttt atcaaaccca tattagagaa gatggatggg
     3301 acggaagagt tgcttgtaaa actcaatcgc gaagatctac tgcgaaagca gcggactttc
     3361 gacaacggta gcattccaca tcaaatccac ttaggcgaat tgcatgctat acttagaagg
     3421 caggaggatt tttatccgtt cctcaaagac aatcgtgaaa agattgagaa aatcctaacc
     3481 tttcgcatac cttactatgt gggacccctg gcccgaggga actctcggtt cgcatggatg
     3541 acaagaaagt ccgaagaaac gattactcca tggaattttg aggaagttgt cgataaaggt
     3601 gcgtcagctc aatcgttcat cgagaggatg accaactttg acaagaattt accgaacgaa
     3661 aaagtattgc ctaagcacag tttactttac gagtatttca cagtgtacaa tgaactcacg
     3721 aaagttaagt atgtcactga gggcatgcgt aaacccgcct ttctaagcgg agaacagaag
     3781 aaagcaatag tagatctgtt attcaagacc aaccgcaaag tgacagttaa gcaattgaaa
     3841 gaggactact ttaagaaaat tgaatgcttc gattctgtcg agatctccgg ggtagaagat
     3901 cgatttaatg cgtcacttgg tacgtatcat gacctcctaa agataattaa agataaggac
     3961 ttcctggata acgaagagaa tgaagatatc ttagaagata tagtgttgac tcttaccctc
     4021 tttgaagatc gggaaatgat tgaggaaaga ctaaaaacat acgctcacct gttcgacgat
     4081 aaggttatga aacagttaaa gaggcgtcgc tatacgggct ggggacgatt gtcgcggaaa
     4141 cttatcaacg ggataagaga caagcaaagt ggtaaaacta ttctcgattt tctaaagagc
     4201 gacggcttcg ccaataggaa ctttatgcag ctgatccatg atgactcttt aaccttcaaa
     4261 gaggatatac aaaaggcaca ggtttccgga caaggggact cattgcacga acatattgcg
     4321 aatcttgctg gttcgccagc catcaaaaag ggcatactcc agacagtcaa agtagtggat
     4381 gagctagtta aggtcatggg acgtcacaaa ccggaaaaca ttgtaatcga gatggcacgc
     4441 gaaaatcaaa cgactcagaa ggggcaaaaa aacagtcgag agcggatgaa gagaatagaa
     4501 gagggtatta aagaactggg cagccagatc ttaaaggagc atcctgtgga aaatacccaa
     4561 ttgcagaacg agaaacttta cctctattac ctacaaaatg gaagggacat gtatgttgat
     4621 caggaactgg acataaaccg tttatctgat tacgacgtcg atcacattgt accccaatcc
     4681 tttttgaagg acgattcaat cgacaataaa gtgcttacac gctcggataa gaaccgaggg
     4741 aaaagtgaca atgttccaag cgaggaagtc gtaaagaaaa tgaagaacta ttggcggcag
     4801 ctcctaaatg cgaaactgat aacgcaaaga aagttcgata acttaactaa agctgagagg
     4861 ggtggcttgt ctgaacttga caaggccgga tttattaaac gtcagctcgt ggaaacccgc
     4921 caaatcacaa agcatgttgc acagatacta gattcccgaa tgaatacgaa atacgacgag
     4981 aacgataagc tgattcggga agtcaaagta atcactttaa agtcaaaatt ggtgtcggac
     5041 ttcagaaagg attttcaatt ctataaagtt agggagataa ataactacca ccatgcgcac
     5101 gacgcttatc ttaatgccgt cgtagggacc gcactcatta agaaataccc gaagctagaa
     5161 agtgagtttg tgtatggtga ttacaaagtt tatgacgtcc gtaagatgat cgcgaaaagc
     5221 gaacaggaga taggcaaggc tacagccaaa tacttctttt attctaacat tatgaatttc
     5281 tttaagacgg aaatcactct ggcaaacgga gagatacgca aacgaccttt aattgaaacc
     5341 aatggggaga caggtgaaat cgtatgggat aagggccggg acttcgcgac ggtgagaaaa
     5401 gttttgtcca tgccccaagt caacatagta aagaaaactg aggtgcagac cggagggttt
     5461 tcaaaggaat cgattcttcc aaaaaggaat agtgataagc tcatcgctcg taaaaaggac
     5521 tgggacccga aaaagtacgg tggcttcgtg agccctacag ttgcctattc tgtcctagta
     5581 gtggcaaaag ttgagaaggg aaaatccaag aaactgaagt cagtcaaaga attattgggg
     5641 ataacgatta tggagcgctc gtcttttgaa aagaacccca tcgacttcct tgaggcgaaa
     5701 ggttacaagg aagtaaaaaa ggatctcata attaaactac caaagtatag tctgtttgag
     5761 ttagaaaatg gccgaaaacg gatgttggct agcgccagag agcttcaaaa ggggaacgaa
     5821 ctcgcactac cgtctaaata cgtgaatttc ctgtatttag cgtcccatta cgagaagttg
     5881 aaaggttcac ctgaagataa cgaacagaag caactttttg ttgagcagca caaacattat
     5941 ctcgacgaaa tcatagagca aatttcggaa ttcagtaaga gagtcatcct agctgatgcc
     6001 aatctggaca aagtattaag cgcatacaac aagcacaggg ataaacccat acgtgagcag
     6061 gcggaaaata ttatccattt gtttactctt accaacctcg gcgctccagc cgcattcaag
     6121 tattttgaca caacgataga tcgcaaagag tacagatcta ccaaggaggt gctagacgcg
     6181 acactgattc accaatccat cacgggatta tatgaaactc ggatagattt gtcacagctt
     6241 gggggtgacg gatcccccaa gaagaagagg aaagtctcga gcgactacaa agaccatgac
     6301 ggtgattata aagatcatga catcgattac aaggatgacg atgacaaggc tgcaggagct
     6361 agcggcagcg gcgccaccaa cttcagcctg ctgaagcagg ccggcgacgt ggaggagaac
     6421 cccggcccca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     6481 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     6541 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     6601 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     6661 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     6721 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     6781 cccaaggagc ccgcgtggtt cctggccacc gtcggagtct cgcccgacca ccagggcaag
     6841 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     6901 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     6961 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     7021 ggtgcctgaa cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     7081 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     7141 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     7201 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     7261 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     7321 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     7381 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     7441 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     7501 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     7561 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     7621 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7681 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     7741 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7801 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7861 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7921 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     7981 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     8041 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     8101 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     8161 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8221 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     8281 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     8341 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     8401 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     8461 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     8521 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     8581 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     8641 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     8701 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     8761 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     8821 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     8881 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     8941 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     9001 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     9061 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     9121 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     9181 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     9241 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     9301 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     9361 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     9421 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     9481 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     9541 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     9601 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     9661 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     9721 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     9781 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     9841 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     9901 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     9961 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    10021 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    10081 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    10141 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    10201 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    10261 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    10321 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    10381 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    10441 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    10501 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    10561 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    10621 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    10681 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    10741 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    10801 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    10861 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    10921 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    10981 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    11041 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    11101 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
    11161 aaagggaaca aaagctggag ctgcaagc
//
