LOCUS       sequence-208419-       13357 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of BE3-P2A-GFP (not 
            codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13357)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 13357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194."
COMMENT     Sequence Label: pLenti-BE3-P2A-GFP-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..13357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2146..2832
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(2881..6279,6280..6282,6283..6879,6880,6881..6883,
                     6884..6885,6886..6888,6889..6981)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(2881..2904,2905..2907,2908..6981)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6994..7242
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7255..7275
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7291..7347
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(7348..7350,7351..7353,7354..8067)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7393..7414)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7654..7673)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8001..8022
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        8079..8578
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8114..8137)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8511..8530
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8599..9198
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8599..8618)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9095..9115
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9214..9802
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9267..9287)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9685..9696)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9874..10107
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10185..10306
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10222..10241)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10276..10295
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10346..10481
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10501..10520)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10502..10520)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10530..10547)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10530..10546)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10539..10561)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10688..11143
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10775..10794)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10985..11006
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11169..11273
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11274..11342,11343..12134)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11492..11511)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12305..12893
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12794..12813
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13047..13064
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13181..13202
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13217..13222,13223..13240,13241..13247)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13255..13271
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13260..13282
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13279..13295
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13279..13295
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13314..13334
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13316..13334
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgag ctcagagact
     2161 ggcccagtgg ctgtggaccc cacattgaga cggcggatcg agccccatga gtttgaggta
     2221 ttcttcgatc cgagagagct ccgcaaggag acctgcctgc tttacgaaat taattggggg
     2281 ggccggcact ccatttggcg acatacatca cagaacacta acaagcacgt cgaagtcaac
     2341 ttcatcgaga agttcacgac agaaagatat ttctgtccga acacaaggtg cagcattacc
     2401 tggtttctca gctggagccc atgcggcgaa tgtagtaggg ccatcactga attcctgtca
     2461 aggtatcccc acgtcactct gtttatttac atcgcaaggc tgtaccacca cgctgacccc
     2521 cgcaatcgac aaggcctgcg ggatttgatc tcttcaggtg tgactatcca aattatgact
     2581 gagcaggagt caggatactg ctggagaaac tttgtgaatt atagcccgag taatgaagcc
     2641 cactggccta ggtatcccca tctgtgggta cgactgtacg ttcttgaact gtactgcatc
     2701 atactgggcc tgcctccttg tctcaacatt ctgagaagga agcagccaca gctgacattc
     2761 tttaccatcg ctcttcagtc ttgtcattac cagcgactgc ccccacacat tctctgggcc
     2821 accgggttga aaagcggcag cgagactccc gggacctcag agtccgccac acccgaaagt
     2881 gataaaaagt attctattgg tttagccatc ggcactaatt ccgttggatg ggctgtcata
     2941 accgatgaat acaaagtacc ttcaaagaaa tttaaggtgt tggggaacac agaccgtcat
     3001 tcgattaaaa agaatcttat cggtgccctc ctattcgata gtggcgaaac ggcagaggcg
     3061 actcgcctga aacgaaccgc tcggagaagg tatacacgtc gcaagaaccg aatatgttac
     3121 ttacaagaaa tttttagcaa tgagatggcc aaagttgacg attctttctt tcaccgtttg
     3181 gaagagtcct tccttgtcga agaggacaag aaacatgaac ggcaccccat ctttggaaac
     3241 atagtagatg aggtggcata tcatgaaaag tacccaacga tttatcacct cagaaaaaag
     3301 ctagttgact caactgataa agcggacctg aggttaatct acttggctct tgcccatatg
     3361 ataaagttcc gtgggcactt tctcattgag ggtgatctaa atccggacaa ctcggatgtc
     3421 gacaaactgt tcatccagtt agtacaaacc tataatcagt tgtttgaaga gaaccctata
     3481 aatgcaagtg gcgtggatgc gaaggctatt cttagcgccc gcctctctaa atcccgacgg
     3541 ctagaaaacc tgatcgcaca attacccgga gagaagaaaa atgggttgtt cggtaacctt
     3601 atagcgctct cactaggcct gacaccaaat tttaagtcga acttcgactt agctgaagat
     3661 gccaaattgc agcttagtaa ggacacgtac gatgacgatc tcgacaatct actggcacaa
     3721 attggagatc agtatgcgga cttatttttg gctgccaaaa accttagcga tgcaatcctc
     3781 ctatctgaca tactgagagt taatactgag attaccaagg cgccgttatc cgcttcaatg
     3841 atcaaaaggt acgatgaaca tcaccaagac ttgacacttc tcaaggccct agtccgtcag
     3901 caactgcctg agaaatataa ggaaatattc tttgatcagt cgaaaaacgg gtacgcaggt
     3961 tatattgacg gcggagcgag tcaagaggaa ttctacaagt ttatcaaacc catattagag
     4021 aagatggatg ggacggaaga gttgcttgta aaactcaatc gcgaagatct actgcgaaag
     4081 cagcggactt tcgacaacgg tagcattcca catcaaatcc acttaggcga attgcatgct
     4141 atacttagaa ggcaggagga tttttatccg ttcctcaaag acaatcgtga aaagattgag
     4201 aaaatcctaa cctttcgcat accttactat gtgggacccc tggcccgagg gaactctcgg
     4261 ttcgcatgga tgacaagaaa gtccgaagaa acgattactc catggaattt tgaggaagtt
     4321 gtcgataaag gtgcgtcagc tcaatcgttc atcgagagga tgaccaactt tgacaagaat
     4381 ttaccgaacg aaaaagtatt gcctaagcac agtttacttt acgagtattt cacagtgtac
     4441 aatgaactca cgaaagttaa gtatgtcact gagggcatgc gtaaacccgc ctttctaagc
     4501 ggagaacaga agaaagcaat agtagatctg ttattcaaga ccaaccgcaa agtgacagtt
     4561 aagcaattga aagaggacta ctttaagaaa attgaatgct tcgattctgt cgagatctcc
     4621 ggggtagaag atcgatttaa tgcgtcactt ggtacgtatc atgacctcct aaagataatt
     4681 aaagataagg acttcctgga taacgaagag aatgaagata tcttagaaga tatagtgttg
     4741 actcttaccc tctttgaaga tcgggaaatg attgaggaaa gactaaaaac atacgctcac
     4801 ctgttcgacg ataaggttat gaaacagtta aagaggcgtc gctatacggg ctggggacga
     4861 ttgtcgcgga aacttatcaa cgggataaga gacaagcaaa gtggtaaaac tattctcgat
     4921 tttctaaaga gcgacggctt cgccaatagg aactttatgc agctgatcca tgatgactct
     4981 ttaaccttca aagaggatat acaaaaggca caggtttccg gacaagggga ctcattgcac
     5041 gaacatattg cgaatcttgc tggttcgcca gccatcaaaa agggcatact ccagacagtc
     5101 aaagtagtgg atgagctagt taaggtcatg ggacgtcaca aaccggaaaa cattgtaatc
     5161 gagatggcac gcgaaaatca aacgactcag aaggggcaaa aaaacagtcg agagcggatg
     5221 aagagaatag aagagggtat taaagaactg ggcagccaga tcttaaagga gcatcctgtg
     5281 gaaaataccc aattgcagaa cgagaaactt tacctctatt acctacaaaa tggaagggac
     5341 atgtatgttg atcaggaact ggacataaac cgtttatctg attacgacgt cgatcacatt
     5401 gtaccccaat cctttttgaa ggacgattca atcgacaata aagtgcttac acgctcggat
     5461 aagaaccgag ggaaaagtga caatgttcca agcgaggaag tcgtaaagaa aatgaagaac
     5521 tattggcggc agctcctaaa tgcgaaactg ataacgcaaa gaaagttcga taacttaact
     5581 aaagctgaga ggggtggctt gtctgaactt gacaaggccg gatttattaa acgtcagctc
     5641 gtggaaaccc gccaaatcac aaagcatgtt gcacagatac tagattcccg aatgaatacg
     5701 aaatacgacg agaacgataa gctgattcgg gaagtcaaag taatcacttt aaagtcaaaa
     5761 ttggtgtcgg acttcagaaa ggattttcaa ttctataaag ttagggagat aaataactac
     5821 caccatgcgc acgacgctta tcttaatgcc gtcgtaggga ccgcactcat taagaaatac
     5881 ccgaagctag aaagtgagtt tgtgtatggt gattacaaag tttatgacgt ccgtaagatg
     5941 atcgcgaaaa gcgaacagga gataggcaag gctacagcca aatacttctt ttattctaac
     6001 attatgaatt tctttaagac ggaaatcact ctggcaaacg gagagatacg caaacgacct
     6061 ttaattgaaa ccaatgggga gacaggtgaa atcgtatggg ataagggccg ggacttcgcg
     6121 acggtgagaa aagttttgtc catgccccaa gtcaacatag taaagaaaac tgaggtgcag
     6181 accggagggt tttcaaagga atcgattctt ccaaaaagga atagtgataa gctcatcgct
     6241 cgtaaaaagg actgggaccc gaaaaagtac ggtggcttcg atagccctac agttgcctat
     6301 tctgtcctag tagtggcaaa agttgagaag ggaaaatcca agaaactgaa gtcagtcaaa
     6361 gaattattgg ggataacgat tatggagcgc tcgtcttttg aaaagaaccc catcgacttc
     6421 cttgaggcga aaggttacaa ggaagtaaaa aaggatctca taattaaact accaaagtat
     6481 agtctgtttg agttagaaaa tggccgaaaa cggatgttgg ctagcgccgg agagcttcaa
     6541 aaggggaacg aactcgcact accgtctaaa tacgtgaatt tcctgtattt agcgtcccat
     6601 tacgagaagt tgaaaggttc acctgaagat aacgaacaga agcaactttt tgttgagcag
     6661 cacaaacatt atctcgacga aatcatagag caaatttcgg aattcagtaa gagagtcatc
     6721 ctagctgatg ccaatctgga caaagtatta agcgcataca acaagcacag ggataaaccc
     6781 atacgtgagc aggcggaaaa tattatccat ttgtttactc ttaccaacct cggcgctcca
     6841 gccgcattca agtattttga cacaacgata gatcgcaaac gatacacttc taccaaggag
     6901 gtgctagacg cgacactgat tcaccaatcc atcacgggat tatatgaaac tcggatagat
     6961 ttgtcacagc ttgggggtga ctctggtggt tctactaatc tgtcagatat tattgaaaag
     7021 gagaccggta agcaactggt tatccaggaa tccatcctca tgctcccaga ggaggtggaa
     7081 gaagtcattg ggaacaagcc ggaaagcgat atactcgtgc acaccgccta cgacgagagc
     7141 accgacgaga atgtcatgct tctgactagc gacgcccctg aatacaagcc ttgggctctg
     7201 gtcatacagg atagcaacgg tgagaacaag attaagatgc tctctggtgg ttctcccaag
     7261 aagaagagga aagtcatgca tggcagcggc gccaccaact tcagcctgct gaagcaggcc
     7321 ggcgacgtgg aggagaaccc cggccccatg gtgagcaagg gcgaggagct gttcaccggg
     7381 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtct
     7441 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     7501 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     7561 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     7621 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     7681 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     7741 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     7801 tatatcatgg ccgacaagca gaagaacggc atcaaggcga acttcaagat ccgccacaac
     7861 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     7921 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     7981 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     8041 ctcggcatgg acgagctgta caagtaagct agcctaccgg gtaggggagg cgcttttccc
     8101 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
     8161 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
     8221 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
     8281 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
     8341 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
     8401 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
     8461 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
     8521 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
     8581 ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     8641 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     8701 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     8761 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     8821 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     8881 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     8941 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     9001 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     9061 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     9121 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     9181 cgcaagcccg gtgcctgatt ctagaaagtc gacaatcaac ctctggatta caaaatttgt
     9241 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     9301 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     9361 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     9421 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     9481 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     9541 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     9601 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     9661 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     9721 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     9781 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     9841 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     9901 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     9961 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
    10021 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
    10081 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
    10141 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
    10201 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    10261 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
    10321 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
    10381 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
    10441 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
    10501 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
    10561 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
    10621 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
    10681 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
    10741 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
    10801 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
    10861 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
    10921 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
    10981 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
    11041 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
    11101 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
    11161 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
    11221 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
    11281 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
    11341 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
    11401 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
    11461 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
    11521 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
    11581 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
    11641 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
    11701 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
    11761 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
    11821 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
    11881 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
    11941 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    12001 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    12061 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    12121 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    12181 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    12241 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    12301 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    12361 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    12421 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    12481 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    12541 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    12601 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    12661 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    12721 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    12781 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    12841 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    12901 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    12961 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    13021 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    13081 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
    13141 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
    13201 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
    13261 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
    13321 accctcacta aagggaacaa aagctggagc tgcaagc
//
