LOCUS       sequence-208422-       12106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12106)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 12106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194."
COMMENT     Sequence Label: pLenti-xBE3-P2A-Puro
FEATURES             Location/Qualifiers
     source          1..12106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2146..2832
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             2881..6981
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 mutant with broad PAM
                     compatibility (Hu et al., 2018)"
                     /label=xCas9-3.7
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDTKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKLYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFIQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6994..7242
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7255..7275
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7291..7347
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7348..7947
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7348..7367)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7844..7864
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7963..8551
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8016..8036)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8434..8445)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8623..8856
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8934..9055
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8971..8990)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9025..9044
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9095..9230
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9250..9269)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9251..9269)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9279..9296)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9279..9295)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9288..9310)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9437..9892
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9524..9543)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9734..9755
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9918..10022
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10023..10091,10092..10883)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10241..10260)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11054..11642
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11543..11562
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11796..11813
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11930..11951
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11966..11971,11972..11989,11990..11996)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12004..12020
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12009..12031
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12028..12044
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12028..12044
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12063..12083
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12065..12083
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgag ctcagagact
     2161 ggcccagtgg ctgtggaccc cacattgaga cggcggatcg agccccatga gtttgaggta
     2221 ttcttcgatc cgagagagct ccgcaaggag acctgcctgc tttacgaaat taattggggg
     2281 ggccggcact ccatttggcg acatacatca cagaacacta acaagcacgt cgaagtcaac
     2341 ttcatcgaga agttcacgac agaaagatat ttctgtccga acacaaggtg cagcattacc
     2401 tggtttctca gctggagccc atgcggcgaa tgtagtaggg ccatcactga attcctgtca
     2461 aggtatcccc acgtcactct gtttatttac atcgcaaggc tgtaccacca cgctgacccc
     2521 cgcaatcgac aaggcctgcg ggatttgatc tcttcaggtg tgactatcca aattatgact
     2581 gagcaggagt caggatactg ctggagaaac tttgtgaatt atagcccgag taatgaagcc
     2641 cactggccta ggtatcccca tctgtgggta cgactgtacg ttcttgaact gtactgcatc
     2701 atactgggcc tgcctccttg tctcaacatt ctgagaagga agcagccaca gctgacattc
     2761 tttaccatcg ctcttcagtc ttgtcattac cagcgactgc ccccacacat tctctgggcc
     2821 accgggttga aaagcggcag cgagactccc gggacctcag agtccgccac acccgaaagt
     2881 gacaagaagt actccattgg gctcgctatc ggcacaaaca gcgtcggctg ggccgtcatt
     2941 acggacgagt acaaggtgcc gagcaaaaaa ttcaaagttc tgggcaatac cgatcgccac
     3001 agcataaaga agaacctcat tggcgccctc ctgttcgact ccggggagac ggccgaagcc
     3061 acgcggctca aaagaacagc acggcgcaga tatacccgca gaaagaatcg gatctgctac
     3121 ctgcaggaga tctttagtaa tgagatggct aaggtggatg actctttctt ccataggctg
     3181 gaggagtcct ttttggtgga ggaggataaa aagcacgagc gccacccaat ctttggcaat
     3241 atcgtggacg aggtggcgta ccatgaaaag tacccaacca tatatcatct gaggaagaag
     3301 cttgtagaca gtactgataa ggctgacttg cggttgatct atctcgcgct ggcgcatatg
     3361 atcaaatttc ggggacactt cctcatcgag ggggacctga acccagacaa cagcgatgtc
     3421 gacaaactct ttatccaact ggttcagact tacaatcagc ttttcgaaga gaacccgatc
     3481 aacgcatccg gagttgacgc caaagcaatc ctgagcgcta ggctgtccaa atcccggcgg
     3541 ctcgaaaacc tcatcgcaca gctccctggg gagaagaaga acggcctgtt tggtaatctt
     3601 atcgccctgt ccctcgggct gacccccaac tttaaatcta acttcgacct ggccgaagat
     3661 accaagcttc aactgagcaa agacacctac gatgatgatc tcgacaatct gctggcccag
     3721 atcggcgacc agtacgcaga cctttttttg gcggcaaaga acctgtcaga cgccattctg
     3781 ctgagtgata ttctgcgagt gaacacggag atcaccaaag ctccgctgag cgctagtatg
     3841 atcaagctct atgatgagca ccaccaagac ttgactttgc tgaaggccct tgtcagacag
     3901 caactgcctg agaagtacaa ggaaattttc ttcgatcagt ctaaaaatgg ctacgccgga
     3961 tacattgacg gcggagcaag ccaggaggaa ttttacaaat ttattaagcc catcttggaa
     4021 aaaatggacg gcaccgagga gctgctggta aagcttaaca gagaagatct gttgcgcaaa
     4081 cagcgcactt tcgacaatgg aatcatcccc caccagattc acctgggcga actgcacgct
     4141 atcctcaggc ggcaagagga tttctacccc tttttgaaag ataacaggga aaagattgag
     4201 aaaatcctca catttcggat accctactat gtaggccccc tcgcccgggg aaattccaga
     4261 ttcgcgtgga tgactcgcaa atcagaagag accatcactc cctggaactt cgagaaagtc
     4321 gtggataagg gggcctctgc ccagtccttc atcgaaagga tgactaactt tgataaaaat
     4381 ctgcctaacg aaaaggtgct tcctaaacac tctctgctgt acgagtactt cacagtttat
     4441 aacgagctca ccaaggtcaa atacgtcaca gaagggatga gaaagccagc attcctgtct
     4501 ggagatcaga agaaagctat tgtggacctc ctcttcaaga cgaaccggaa agttaccgtg
     4561 aaacagctca aagaagacta tttcaaaaag attgaatgtt tcgactctgt tgaaatcagc
     4621 ggagtggagg atcgcttcaa cgcatccctg ggaacgtatc acgatctcct gaaaatcatt
     4681 aaagacaagg acttcctgga caatgaggag aacgaggaca ttcttgagga cattgtcctc
     4741 acccttacgt tgtttgaaga tagggagatg attgaagaac gcttgaaaac ttacgctcat
     4801 ctcttcgacg acaaagtcat gaagcagctc aagaggcgcc gatatacagg atgggggcgg
     4861 ctgtcaagaa aactgatcaa tgggatccga gacaagcaga gtggaaagac aatcctggat
     4921 tttcttaagt ccgatggatt tgccaaccgg aacttcattc agttgatcca tgatgactct
     4981 ctcaccttta aggaggacat ccagaaagca caagtttctg gccaggggga cagtcttcac
     5041 gagcacatcg ctaatcttgc aggtagccca gctatcaaaa agggaatact gcagaccgtt
     5101 aaggtcgtgg atgaactcgt caaagtaatg ggaaggcata agcccgagaa tatcgttatc
     5161 gagatggccc gagagaacca aaccacccag aagggacaga agaacagtag ggaaaggatg
     5221 aagaggattg aagagggtat aaaagaactg gggtcccaaa tccttaagga acacccagtt
     5281 gaaaacaccc agcttcagaa tgagaagctc tacctgtact acctgcagaa cggcagggac
     5341 atgtacgtgg atcaggaact ggacatcaat cggctctccg actacgacgt ggatcatatc
     5401 gtgccccagt cttttctcaa agatgattct attgataata aagtgttgac aagatccgat
     5461 aaaaacagag ggaagagtga taacgtcccc tcagaagaag ttgtcaagaa aatgaaaaat
     5521 tattggcggc agctgctgaa cgccaaactg atcacacaac ggaagttcga taatctgact
     5581 aaggctgaac gaggtggcct gtctgagttg gataaagccg gtttcatcaa aaggcagctt
     5641 gttgagacac gccagatcac caagcacgtg gcccaaattc tcgattcacg catgaacacc
     5701 aagtacgatg aaaatgacaa actgattcga gaggtgaaag ttattactct gaagtctaag
     5761 ctggtctcag atttcagaaa ggactttcag ttttataagg tgagagagat caacaattac
     5821 caccatgcgc atgatgccta cctgaatgca gtggtaggca ctgcacttat caaaaaatat
     5881 cccaagcttg aatctgaatt tgtttacgga gactataaag tgtacgatgt taggaaaatg
     5941 atcgcaaagt ctgagcagga aataggcaag gccaccgcta agtacttctt ttacagcaat
     6001 attatgaatt ttttcaagac cgagattaca ctggccaatg gagagattcg gaagcgacca
     6061 cttatcgaaa caaacggaga aacaggagaa atcgtgtggg acaagggtag ggatttcgcg
     6121 acagtccgga aggtcctgtc catgccgcag gtgaacatcg ttaaaaagac cgaagtacag
     6181 accggaggct tctccaagga aagtatcctc ccgaaaagga acagcgacaa gctgatcgca
     6241 cgcaaaaaag attgggaccc caagaaatac ggcggattcg attctcctac agtcgcttac
     6301 agtgtactgg ttgtggctaa agtggagaaa gggaagtcta aaaaactcaa aagcgtcaag
     6361 gaactgctgg gcatcacaat catggagcga tcaagcttcg aaaaaaaccc catcgacttt
     6421 ctcgaggcga aaggatataa agaggtcaaa aaagacctca tcattaagct tcccaagtac
     6481 tctctctttg agcttgaaaa cggccggaaa cgaatgctcg ctagtgcggg cgtgctgcag
     6541 aaaggtaacg agctggcact gccctctaaa tacgttaatt tcttgtatct ggccagccac
     6601 tatgaaaagc tcaaagggtc tcccgaagat aatgagcaga agcagctgtt cgtggaacaa
     6661 cacaaacact accttgatga gatcatcgag caaataagcg aattctccaa aagagtgatc
     6721 ctcgccgacg ctaacctcga taaggtgctt tctgcttaca ataagcacag ggataagccc
     6781 atcagggagc aggcagaaaa cattatccac ttgtttactc tgaccaactt gggcgcgcct
     6841 gcagccttca agtacttcga cactaccata gacagaaagc ggtacacctc tacaaaggag
     6901 gtcctggacg ccacactgat tcatcagtca attacggggc tctatgaaac aagaatcgac
     6961 ctctctcagc tcggtggaga ctctggtggt tctactaatc tgtcagatat tattgaaaag
     7021 gagaccggta agcaactggt tatccaggaa tccatcctca tgctcccaga ggaggtggaa
     7081 gaagtcattg ggaacaagcc ggaaagcgat atactcgtgc acaccgccta cgacgagagc
     7141 accgacgaga atgtcatgct tctgactagc gacgcccctg aatacaagcc ttgggctctg
     7201 gtcatacagg atagcaacgg tgagaacaag attaagatgc tctctggtgg ttctcccaag
     7261 aagaagagga aagtcgctag cggcagcggc gccaccaact tcagcctgct gaagcaggcc
     7321 ggcgacgtgg aggagaaccc cggccccatg accgagtaca agcccacggt gcgcctcgcc
     7381 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     7441 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     7501 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     7561 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     7621 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     7681 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggagtctcg
     7741 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     7801 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     7861 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     7921 tgcatgaccc gcaagcccgg tgcctgaacg cgttaagtcg acaatcaacc tctggattac
     7981 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     8041 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     8101 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     8161 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     8221 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     8281 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     8341 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     8401 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     8461 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     8521 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     8581 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     8641 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     8701 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     8761 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     8821 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     8881 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     8941 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     9001 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     9061 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     9121 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     9181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     9241 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     9301 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     9361 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     9421 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     9481 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     9541 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     9601 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     9661 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     9721 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     9781 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     9841 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     9901 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     9961 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    10021 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
    10081 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
    10141 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
    10201 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
    10261 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
    10321 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
    10381 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    10441 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    10501 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    10561 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    10621 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    10681 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    10741 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    10801 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    10861 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    10921 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    10981 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    11041 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    11101 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    11161 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    11221 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    11281 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    11341 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    11401 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    11461 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    11521 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    11581 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    11641 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    11701 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    11761 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    11821 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    11881 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    11941 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    12001 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
    12061 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagc
//
