LOCUS       sequence-208429-        9504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  W1.2 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1, PRha-sgRNA2 
            (sgRNA1-G19T-G20C) and is part of CAMERA 1.2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Tang W, Liu DR
  TITLE     Rewritable multi-event analog recording in bacterial and mammalian 
            cells.
  JOURNAL   Science. 2018 Feb 15. pii: science.aap8992. doi: 
            10.1126/science.aap8992.
  PUBMED    29449507
REFERENCE   2  (bases 1 to 9504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992."
COMMENT     Sequence Label: pWT019b
FEATURES             Location/Qualifiers
     source          1..9504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..951)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTVKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      999..1221
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      1716..1762
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    1796..1814
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        1815..1869
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    1820..1838
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    1850..1868
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             1926..6029
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1926..6026
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1926..6026
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_RNA        complement(6143..6218)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(6239..6357)
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     CDS             6503..7339
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     CDS             7413..8261
                     /codon_start=1
                     /gene="E. coli rhaR"
                     /product="transcriptional activator of rhaR and rhaS"
                     /label=rhaR
                     /note="needed for rhamnose-dependent activation of the rhaB
                     promoter"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
                     RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI
                     PGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHR
                     YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
                     QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
                     D"
     terminator      8269..8296
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(8356..8401)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     misc_RNA        complement(8409..8484)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    8517..8533
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    8540..8556
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(8614..9405)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(9406..9477)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
       61 ccatgagaac gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgcctc
      121 aaaactggtg agctgaattt ttacagttaa agcatcgtgt agtgtttttc ttagtccgtt
      181 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
      241 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
      301 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
      361 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
      421 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt
      481 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc
      541 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg
      601 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt
      661 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat
      721 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat
      781 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat
      841 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc
      901 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa
      961 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact
     1021 agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact
     1081 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta
     1141 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga
     1201 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc
     1261 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac
     1321 cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga
     1381 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg
     1441 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag
     1501 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct
     1561 gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta
     1621 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca
     1681 ggcttacccg tcttactgtc aagaggacat ccggtcaaat aaaacgaaag gctcagtcga
     1741 aagactgggc ctttcgtttt attctagagc acagctccat cgaatggcca gatgatccct
     1801 atcagtgata gagattgaca ctctatcatt gatagagtta ttttaccact ccctatcagt
     1861 gatagagaaa agtgaaatga atagttcgac aaaaatctag acctgcaggt gcagtaagga
     1921 aattggacaa gaagtactcc attgggctcg atatcggcac aaacagcgtc ggctgggccg
     1981 tcattacgga cgagtacaag gtgccgagca aaaaattcaa agttctgggc aataccgatc
     2041 gccacagcat aaagaagaac ctcattggcg ccctcctgtt cgactccggg gagacggccg
     2101 aagccacgcg gctcaaaaga acagcacggc gcagatatac ccgcagaaag aatcggatct
     2161 gctacctgca ggagatcttt agtaatgaga tggctaaggt ggatgactct ttcttccata
     2221 ggctggagga gtcctttttg gtggaggagg ataaaaagca cgagcgccac ccaatctttg
     2281 gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc aaccatatat catctgagga
     2341 agaagcttgt agacagtact gataaggctg acttgcggtt gatctatctc gcgctggcgc
     2401 atatgatcaa atttcgggga cacttcctca tcgaggggga cctgaaccca gacaacagcg
     2461 atgtcgacaa actctttatc caactggttc agacttacaa tcagcttttc gaagagaacc
     2521 cgatcaacgc atccggagtt gacgccaaag caatcctgag cgctaggctg tccaaatccc
     2581 ggcggctcga aaacctcatc gcacagctcc ctggggagaa gaagaacggc ctgtttggta
     2641 atcttatcgc cctgtcactc gggctgaccc ccaactttaa atctaacttc gacctggccg
     2701 aagatgccaa gcttcaactg agcaaagaca cctacgatga tgatctcgac aatctgctgg
     2761 cccagatcgg cgaccagtac gcagaccttt ttttggcggc aaagaacctg tcagacgcca
     2821 ttctgctgag tgatattctg cgagtgaaca cggagatcac caaagctccg ctgagcgcta
     2881 gtatgatcaa gcgctatgat gagcaccacc aagacttgac tttgctgaag gcccttgtca
     2941 gacagcaact gcctgagaag tacaaggaaa ttttcttcga tcagtctaaa aatggctacg
     3001 ccggatacat tgacggcgga gcaagccagg aggaatttta caaatttatt aagcccatct
     3061 tggaaaaaat ggacggcacc gaggagctgc tggtaaagct taacagagaa gatctgttgc
     3121 gcaaacagcg cactttcgac aatggaagca tcccccacca gattcacctg ggcgaactgc
     3181 acgctatcct caggcggcaa gaggatttct accccttttt gaaagataac agggaaaaga
     3241 ttgagaaaat cctcacattt cggataccct actatgtagg ccccctcgcc cggggaaatt
     3301 ccagattcgc gtggatgact cgcaaatcag aagagaccat cactccctgg aacttcgagg
     3361 aagtcgtgga taagggggcc tctgcccagt ccttcatcga aaggatgact aactttgata
     3421 aaaatctgcc taacgaaaag gtgcttccta aacactctct gctgtacgag tacttcacag
     3481 tttataacga gctcaccaag gtcaaatacg tcacagaagg gatgagaaag ccagcattcc
     3541 tgtctggaga gcagaagaaa gctatcgtgg acctcctctt caagacgaac cggaaagtta
     3601 ccgtgaaaca gctcaaagaa gactatttca aaaagattga atgtttcgac tctgttgaaa
     3661 tcagcggagt ggaggatcgc ttcaacgcat ccctgggaac gtatcacgat ctcctgaaaa
     3721 tcattaaaga caaggacttc ctggacaatg aggagaacga ggacattctt gaggacattg
     3781 tcctcaccct tacgttgttt gaagataggg agatgattga agaacgcttg aaaacttacg
     3841 ctcatctctt cgacgacaaa gtcatgaaac agctcaagag gcgccgatat acaggatggg
     3901 ggcggctgtc aagaaaactg atcaatggga tccgagacaa gcagagtgga aagacaatcc
     3961 tggattttct taagtccgat ggatttgcca accggaactt catgcagttg atccatgatg
     4021 actctctcac ctttaaggag gacatccaga aagcacaagt ttctggccag ggggacagtc
     4081 ttcacgagca catcgctaat cttgcaggta gcccagctat caaaaaggga atactgcaga
     4141 ccgttaaggt cgtggatgaa ctcgtcaaag taatgggaag gcataagccc gagaatatcg
     4201 ttatcgagat ggcccgagag aaccaaacta cccagaaggg acagaagaac agtagggaaa
     4261 ggatgaagag gattgaagag ggtataaaag aactggggtc ccaaatcctt aaggaacacc
     4321 cagttgaaaa cacccagctt cagaatgaga agctctacct gtactacctg cagaacggca
     4381 gggacatgta cgtggatcag gaactggaca tcaatcggct ctccgactac gacgtggacc
     4441 atatcgtgcc ccagtctttt ctcaaagatg attctattga taataaagtg ttgacaagat
     4501 ccgataaaaa cagagggaag agtgataacg tcccctcaga agaagttgtc aagaaaatga
     4561 aaaattattg gcggcagctg ctgaacgcca aactgatcac acaacggaag ttcgataatc
     4621 tgactaaggc tgaacgaggt ggcctgtctg agttggataa agccggcttc atcaaaaggc
     4681 agcttgttga gacacgccag atcaccaagc acgtggccca aattctcgat tcacgcatga
     4741 acaccaagta cgatgaaaat gacaaactga ttcgagaggt gaaagttatt actctgaagt
     4801 ctaagctggt ctcagatttc agaaaggact ttcagtttta taaggtgaga gagatcaaca
     4861 attaccacca tgcgcatgat gcctacctga atgcagtggt aggcactgca cttatcaaaa
     4921 aatatcccaa gcttgaatct gaatttgttt acggagacta taaagtgtac gatgttagga
     4981 aaatgatcgc aaagtctgag caggaaatag gcaaggccac cgctaagtac ttcttttaca
     5041 gcaatattat gaattttttc aagaccgaga ttacactggc caatggagag attcggaagc
     5101 gaccacttat cgaaacaaac ggagaaacag gagaaatcgt gtgggacaag ggtagggatt
     5161 tcgcgacagt ccggaaggtc ctgtccatgc cgcaggtgaa catcgttaaa aagaccgaag
     5221 tacagaccgg aggcttctcc aaggaaagta tcctcccgaa aaggaacagc gacaagctga
     5281 tcgcacgcaa aaaagattgg gaccccaaga aatacggcgg attcgattct cctacagtcg
     5341 cttacagtgt actggttgtg gccaaagtgg agaaagggaa gtctaaaaaa ctcaaaagcg
     5401 tcaaggaact gctgggcatc acaatcatgg agcgatcaag cttcgaaaaa aaccccatcg
     5461 actttctcga ggcgaaagga tataaagagg tcaaaaaaga cctcatcatt aagcttccca
     5521 agtactctct ctttgagctt gaaaacggcc ggaaacgaat gctcgctagt gcgggcgagc
     5581 tgcagaaagg taacgagctg gcactgccct ctaaatacgt taatttcttg tatctggcca
     5641 gccactatga aaagctcaaa gggtctcccg aagataatga gcagaagcag ctgttcgtgg
     5701 aacaacacaa acactacctt gatgagatca tcgagcaaat aagcgaattc tccaaaagag
     5761 tgatcctcgc cgacgctaac ctcgataagg tgctttctgc ttacaataag cacagggata
     5821 agcccatcag ggagcaggca gaaaacatta tccacttgtt tactctgacc aacttgggcg
     5881 cgcctgcagc cttcaagtac ttcgacacca ccatagacag aaagcggtac acctctacaa
     5941 aggaggtcct ggacgccaca ctgattcatc agtcaattac ggggctctat gaaacaagaa
     6001 tcgacctctc tcagctcggt ggagactgaa cggagccaat gtacgcaaaa accccgcttc
     6061 agcggggttt tttcgccaaa aaaaaccccg ccctgtcagg ggcggggttt ttttttatgt
     6121 tgaaaatctc cttctaaaaa aagcaccgac tcggtgccac tttttcaagt tgataacgga
     6181 ctagccttat tttaacttgc tatttctagc tctaaaacga ggcaagctgc ccgtgcccta
     6241 cgaccagtct aaaaagcgcc tgaattcgcg accttctcgt tactgacagg aaaatgggcc
     6301 attggcaacc agggaaagat gaacgtgatg atgttcacaa tttgctgaat tgtggtgatg
     6361 tgatgctcac cgcatttcct gaaaattcac gctgtatctt gaaaaatcga cgttttttac
     6421 gtggttttcc gtcgaaaatt taaggtaaga acctgacctc gtgattacta tttcgccgtg
     6481 ttgacgacat caggaggcca gtatgaccgt attacatagt gtggattttt ttccgtctgg
     6541 taacgcgtcc gtggcgatag aaccccggct cccgcaggcg gattttcctg aacatcatca
     6601 tgattttcat gaaattgtga ttgtcgaaca tggcacgggt attcatgtgt ttaatgggca
     6661 gccctatacc atcaccggtg gcacggtctg tttcgtacgc gatcatgatc ggcatctgta
     6721 tgaacatacc gataatctgt gtctgaccaa tgtgctgtat cgctcgccgg atcgatttca
     6781 gtttctcgcc gggctgaatc agttgctgcc acaagagctg gatgggcagt atccgtctca
     6841 ctggcgcgtt aaccacagcg tattgcagca ggtgcgacag ctggttgcac agatggaaca
     6901 gcaggaaggg gaaaatgatt taccctcgac cgccagtcgc gagatcttgt ttatgcaatt
     6961 actgctcttg ctgcgtaaaa gcagtttgca ggagaacctg gaaaacagcg catcacgtct
     7021 caacttgctt ctggcctggc tggaggacca ttttgccgat gaggtgaatt gggatgccgt
     7081 ggcggatcaa ttttctcttt cactgcgtac gctacatcgg cagcttaagc agcaaacggg
     7141 actgacgcct cagcgatacc tgaaccgcct gcgactgatg aaagcccgac atctgctacg
     7201 ccacagcgag gccagcgtta ctgacatcgc ctatcgctgt ggattcagcg acagtaacca
     7261 cttttcgacg ctttttcgcc gagagtttaa ctggtcaccg cgtgatattc gccagggacg
     7321 ggatggcttt ctgcaataac gcgaatcttc tcaacgtatt tgtacgccat attgcgaata
     7381 atcaacttcg ttctctggcc gaggtagcca cggtggcgca tcagttaaaa cttctcaaag
     7441 atgatttttt tgccagcgac cagcaggcag tcgctgtggc tgaccgttat ccgcaagatg
     7501 tctttgctga acatacacat gatttttgtg agctggtgat tgtctggcgc ggtaatggcc
     7561 tgcatgtact caacgatcgc ccttatcgca ttacccgtgg cgatctcttt tacattcatg
     7621 ctgacgataa acactcctac gcttccgtta acgatctggt tttgcagaat attatttatt
     7681 gcccggagcg tctgaagctg aatcttgact ggcagggggc gattccggga tttaacgcca
     7741 gcgcagggca accacactgg cgcttaggta gcatggggat ggcgcaggcg cggcaggtta
     7801 tcggtcagct tgagcatgaa agtagtcagc atgtgccgtt tgctaacgaa atggctgagt
     7861 tgctgttcgg gcagttggtg atgttgctga atcgccatcg ttacaccagt gattcgttgc
     7921 cgccaacatc cagcgaaacg ttgctggata agctgattac ccggctggcg gctagcctga
     7981 aaagtccctt tgcgctggat aaattttgtg atgaggcatc gtgcagtgag cgcgttttgc
     8041 gtcagcaatt tcgccagcag actggaatga ccatcaatca atatctgcga caggtcagag
     8101 tgtgtcatgc gcaatatctt ctccagcata gccgcctgtt aatcagtgat atttcgaccg
     8161 aatgtggctt tgaagatagt aactattttt cggtggtgtt tacccgggaa accgggatga
     8221 cgcccagcca gtggcgtcat ctcaattcgc agaaagatta atcacactgg ctcaccttcg
     8281 ggtgggcctt tctgcgttta tatactagag agagaatata aaaagccaga ttattaatcc
     8341 ggctttttta ttatttaaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
     8401 gaaaaaaagc accgactcgg tgccactttt tcaagttgat aacggactag ccttatttta
     8461 acttgctatt tctagctcta aaacccggca agctgcccgt gcccgtgctc agtatcttgt
     8521 tatccgctca caatgtcaat tgttatccgc tcacaattaa atataatgac cctcttgata
     8581 acccaagagg gcatttttta atgcccatgg cgtttatttg ccgactacct tggtgatctc
     8641 gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc
     8701 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag
     8761 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
     8821 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
     8881 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
     8941 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
     9001 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
     9061 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
     9121 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
     9181 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
     9241 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga
     9301 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
     9361 ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatactct tcctttttca
     9421 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttaaatgtat
     9481 ttagaaaaat aggccaaatg ccgt
//
